##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938264_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 990515 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.988134455308604 33.0 31.0 34.0 30.0 34.0 2 32.08287305088767 33.0 31.0 34.0 30.0 34.0 3 32.098250909880214 34.0 31.0 34.0 30.0 34.0 4 35.626773950924516 37.0 35.0 37.0 33.0 37.0 5 35.637290702311425 37.0 35.0 37.0 35.0 37.0 6 35.68143642448625 37.0 35.0 37.0 35.0 37.0 7 35.62260036445687 37.0 35.0 37.0 33.0 37.0 8 35.61621479735289 37.0 35.0 37.0 33.0 37.0 9 37.24420326799695 39.0 37.0 39.0 34.0 39.0 10-11 37.15021983513627 39.0 37.0 39.0 34.0 39.0 12-13 36.99632211526327 39.0 37.0 39.0 33.0 39.0 14-15 38.149366743562695 40.0 38.0 41.0 33.0 41.0 16-17 37.98447423814884 40.0 38.0 41.0 33.0 41.0 18-19 38.1864338248285 40.0 38.0 41.0 33.5 41.0 20-21 38.203260425132385 40.0 38.0 41.0 33.5 41.0 22-23 38.081590384799824 40.0 38.0 41.0 33.0 41.0 24-25 37.94950404587512 40.0 38.0 41.0 33.0 41.0 26-27 37.75971943887775 40.0 38.0 41.0 32.5 41.0 28-29 37.57911692402437 40.0 37.0 41.0 32.0 41.0 30-31 37.34876503636997 40.0 37.0 41.0 32.0 41.0 32-33 37.03098792042523 40.0 36.0 41.0 31.0 41.0 34-35 36.77328662362508 39.0 36.0 41.0 30.5 41.0 36-37 36.49653311661106 39.0 35.0 41.0 30.0 41.0 38-39 36.20453249067404 39.0 35.0 40.5 30.0 41.0 40-41 36.08480638859583 39.0 35.0 40.5 29.5 41.0 42-43 35.82914292060191 39.0 35.0 40.5 28.5 41.0 44-45 35.460268143339576 39.0 35.0 40.0 26.5 41.0 46-47 35.011381453082485 38.0 34.5 40.0 24.5 41.0 48-49 34.56663806201824 38.0 34.0 40.0 23.5 41.0 50-51 33.83370569855075 37.5 33.5 39.5 22.5 40.5 52-53 33.913544469291224 38.0 33.5 40.0 23.0 40.5 54-55 34.004369948965945 38.0 34.0 40.0 21.5 41.0 56-57 33.68933837448196 38.0 33.0 40.0 19.5 41.0 58-59 33.2811850401054 37.5 33.0 40.0 17.0 41.0 60-61 32.76353210198735 37.0 32.0 40.0 11.5 41.0 62-63 32.21759337314427 36.0 31.0 39.0 8.0 41.0 64-65 31.674150820532752 35.5 31.0 39.0 4.5 40.0 66-67 31.094344861006647 35.0 30.5 38.5 2.0 40.0 68-69 30.401906079160838 35.0 29.0 38.0 2.0 40.0 70-71 29.80161431174692 34.5 28.5 37.5 2.0 39.5 72-73 29.093374658637174 34.0 27.5 37.0 2.0 39.0 74-75 28.41413254721029 34.0 26.0 36.0 2.0 39.0 76-77 27.662531612343074 33.5 25.5 35.5 2.0 38.0 78-79 26.88510724219219 33.0 24.5 35.0 2.0 37.0 80-81 26.128489725042023 32.0 21.5 35.0 2.0 36.5 82-83 25.38234403315447 32.0 19.0 35.0 2.0 36.0 84-85 24.63716803884848 31.0 15.5 34.5 2.0 35.5 86-87 23.880698929344838 31.0 7.0 34.0 2.0 35.0 88-89 22.992906215453576 30.0 2.0 34.0 2.0 35.0 90-91 22.065742063472033 29.0 2.0 34.0 2.0 35.0 92-93 21.118797797105547 29.0 2.0 34.0 2.0 35.0 94-95 20.03057247997254 27.5 2.0 34.0 2.0 35.0 96-97 18.7602615810967 25.5 2.0 33.0 2.0 35.0 98-99 17.206902974715174 21.0 2.0 33.0 2.0 34.5 100-101 13.36106368909103 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10796188954723007 1101 2 0.03396129743063625 1101 3 0.06618546961481542 1101 4 -0.10230336853069133 1101 5 7.047366240016117E-4 1101 6 -0.02033076821219737 1101 7 0.13324414039045251 1101 8 0.1655156222319647 1101 9 0.16891052604736245 1101 10-11 0.07924741386300838 1101 12-13 0.18222333202673013 1101 14-15 0.10199506490038601 1101 16-17 0.40706183296538256 1101 18-19 0.2835793235924484 1101 20-21 0.16058800946553475 1101 22-23 0.12097681758032763 1101 24-25 0.1622559299256281 1101 26-27 0.11864604039808313 1101 28-29 0.1784678127303394 1101 30-31 0.021611765862111554 1101 32-33 0.28341471214506697 1101 34-35 0.18522338928557502 1101 36-37 0.10327080120737975 1101 38-39 0.37821699280234355 1101 40-41 0.5001690815730768 1101 42-43 0.3427665654940171 1101 44-45 0.29451512528384427 1101 46-47 0.3343799909867258 1101 48-49 0.5163488883527307 1101 50-51 0.45170349137723775 1101 52-53 0.5130349482996053 1101 54-55 0.36934623012234624 1101 56-57 0.4296453178343427 1101 58-59 0.3577516550082791 1101 60-61 0.37844831255793565 1101 62-63 0.15236556122962952 1101 64-65 0.31228476822777296 1101 66-67 0.32106113276813275 1101 68-69 0.2926384605478134 1101 70-71 0.21745152248179878 1101 72-73 -0.058829837778585414 1101 74-75 0.09775827818272731 1101 76-77 -0.4741501759010518 1101 78-79 -0.5868654046407009 1101 80-81 -0.4548962431596557 1101 82-83 -0.5353937730722755 1101 84-85 -0.6455703758047839 1101 86-87 -0.6210055265455559 1101 88-89 -0.5999151926542119 1101 90-91 -0.4045168583590417 1101 92-93 -0.5448900695703287 1101 94-95 -0.567097991713176 1101 96-97 -0.43036394301824643 1101 98-99 -0.5433725630822295 1101 100-101 -0.5705552817826902 1102 1 0.05839895481387103 1102 2 0.09059545070551422 1102 3 0.020702254875409665 1102 4 -0.09960058179544262 1102 5 -0.15703407246198253 1102 6 -0.09672835546979286 1102 7 0.07944380459640854 1102 8 -0.0039071318122196885 1102 9 0.16498626626002988 1102 10-11 0.02084878724289041 1102 12-13 -0.010296367002311513 1102 14-15 0.11416426778928468 1102 16-17 0.15145749251640694 1102 18-19 -0.03439121003581391 1102 20-21 0.1447488352701498 1102 22-23 0.21125144913963823 1102 24-25 0.11287177442988394 1102 26-27 0.07280859847357846 1102 28-29 0.1015771945317212 1102 30-31 0.1630672287337731 1102 32-33 0.23129667787275565 1102 34-35 0.1948452656458457 1102 36-37 0.1191777116356505 1102 38-39 -0.19969966276001827 1102 40-41 0.23508388937984392 1102 42-43 0.27701022938016706 1102 44-45 0.2137380019195092 1102 46-47 0.09980497477445027 1102 48-49 -0.16025945852785384 1102 50-51 -0.1843863735056388 1102 52-53 -0.3047201618994748 1102 54-55 -0.12641414795687922 1102 56-57 -0.407548980773619 1102 58-59 -0.4215981215731972 1102 60-61 -0.18321942012158132 1102 62-63 -0.14195947497039185 1102 64-65 -0.4157664714563296 1102 66-67 -0.6392976249715616 1102 68-69 -0.5844036203092635 1102 70-71 -0.5779650664547624 1102 72-73 -0.4446087822481992 1102 74-75 -0.702855123375425 1102 76-77 -0.7753143507626916 1102 78-79 -0.5861747171512484 1102 80-81 -0.7227475166008297 1102 82-83 -0.8248740029757116 1102 84-85 -0.9984095233498458 1102 86-87 -0.7049440442546953 1102 88-89 -1.0023383731035374 1102 90-91 -1.0073004844017355 1102 92-93 -1.169481431312903 1102 94-95 -1.2217020567754986 1102 96-97 -1.3769889019323394 1102 98-99 -1.2030768333520179 1102 100-101 -1.1005146844601583 1103 1 0.3073529297092641 1103 2 0.2631895929649275 1103 3 0.2372294515281297 1103 4 0.14412326757693705 1103 5 0.1337609066175176 1103 6 0.24741390394443386 1103 7 0.20496681714870846 1103 8 0.07559077613757381 1103 9 0.30933773069517656 1103 10-11 0.2504512977031368 1103 12-13 0.2574860597759283 1103 14-15 0.2595617573290312 1103 16-17 0.13158301552896745 1103 18-19 0.27366318326962613 1103 20-21 0.28805427460068955 1103 22-23 0.22537278805177152 1103 24-25 0.030235791166283832 1103 26-27 0.13609311730202478 1103 28-29 0.1387110857451077 1103 30-31 0.2572094881479998 1103 32-33 0.17742638498572205 1103 34-35 -0.16446435778511415 1103 36-37 -0.20352103313422276 1103 38-39 -0.01089213137927203 1103 40-41 0.024832843354744227 1103 42-43 0.1901901456981605 1103 44-45 0.3021271232584226 1103 46-47 0.3770016274941099 1103 48-49 0.5290710854051994 1103 50-51 0.5023291076659078 1103 52-53 0.4316815117406918 1103 54-55 0.48969463865400087 1103 56-57 0.5678694292682209 1103 58-59 0.32997091608370965 1103 60-61 0.1755671907570786 1103 62-63 -0.14980466325490482 1103 64-65 -0.26461584384126624 1103 66-67 -0.21618047015984487 1103 68-69 -0.3924580136147 1103 70-71 -0.40223284888154254 1103 72-73 -0.3488974851770692 1103 74-75 -0.6097588890658052 1103 76-77 -0.543096777540935 1103 78-79 -0.5343964744734251 1103 80-81 -0.5841491902409999 1103 82-83 -0.6831376013020716 1103 84-85 -0.8289534564042391 1103 86-87 -0.8723080609910951 1103 88-89 -0.6032797956976772 1103 90-91 -0.7850201496737022 1103 92-93 -0.911113230476083 1103 94-95 -1.0872878308340717 1103 96-97 -1.6390181906352659 1103 98-99 -1.5796458710089212 1103 100-101 -1.269447738853465 1104 1 0.1759024415642152 1104 2 0.1538211439912729 1104 3 0.3786781428202417 1104 4 0.23095832503344838 1104 5 0.2086554484627996 1104 6 0.08576772995773041 1104 7 0.23464697732531192 1104 8 0.2215444344445956 1104 9 0.2120702306310065 1104 10-11 0.3128256249528434 1104 12-13 0.41274057116028473 1104 14-15 0.35899296780896606 1104 16-17 0.35360879609044105 1104 18-19 0.3825952536720365 1104 20-21 0.1895011690398576 1104 22-23 0.41347548247554045 1104 24-25 0.4228748975314218 1104 26-27 0.24269355710317342 1104 28-29 0.15237465497956748 1104 30-31 0.411644720155941 1104 32-33 0.42541776009161225 1104 34-35 0.33020925671292645 1104 36-37 0.27676278406593724 1104 38-39 0.08931985448548829 1104 40-41 0.3321021830540616 1104 42-43 0.2833837095420506 1104 44-45 0.5740925808997517 1104 46-47 0.18866470045490047 1104 48-49 0.05219808012586924 1104 50-51 0.11883256019049071 1104 52-53 0.012680005806799954 1104 54-55 -0.024320814281296066 1104 56-57 -0.1442576167911156 1104 58-59 0.002646344547571289 1104 60-61 -0.07556546832857691 1104 62-63 -0.05983794781651852 1104 64-65 -0.021312329710301725 1104 66-67 -0.099601079207428 1104 68-69 -0.10184592849521223 1104 70-71 -0.021597361891615208 1104 72-73 -0.10410942825961556 1104 74-75 -0.2427260066569943 1104 76-77 -0.4777191389479576 1104 78-79 -0.21688507301369597 1104 80-81 -0.58353847415232 1104 82-83 -0.5255888937527224 1104 84-85 -0.7642025772297636 1104 86-87 -0.024002022709574078 1104 88-89 -0.4346943467839175 1104 90-91 -0.7818467790342787 1104 92-93 -0.9519114182215489 1104 94-95 -1.0938912653901234 1104 96-97 -1.2089970562221133 1104 98-99 -1.13652054479455 1104 100-101 -1.107225074548376 1105 1 -0.043449128851182905 1105 2 0.06337230292241003 1105 3 0.03885243313420261 1105 4 0.07415298444009721 1105 5 0.08957985843041882 1105 6 0.176179276380239 1105 7 0.13574157519578733 1105 8 0.12473064039806303 1105 9 0.11877145700329095 1105 10-11 0.050675339959589394 1105 12-13 0.22762082426660868 1105 14-15 0.1437173914512826 1105 16-17 0.11555085264155451 1105 18-19 0.22498963212567702 1105 20-21 0.11952553487547135 1105 22-23 0.137014930887716 1105 24-25 0.07827081128133528 1105 26-27 0.015199322233925727 1105 28-29 -0.0279900245040281 1105 30-31 -0.10294214839907312 1105 32-33 0.05301828470296499 1105 34-35 0.04717396976169397 1105 36-37 0.0803163422859896 1105 38-39 0.13943608946662067 1105 40-41 -0.10465542039195697 1105 42-43 0.07063329153122311 1105 44-45 0.2514892281110477 1105 46-47 0.3947782253584009 1105 48-49 0.24046300886201522 1105 50-51 0.15465717590024042 1105 52-53 0.26880098963957266 1105 54-55 0.02283360420193503 1105 56-57 0.20069766071213024 1105 58-59 0.12713742801920347 1105 60-61 0.3877878797982959 1105 62-63 0.42524419364207233 1105 64-65 0.15225695441896292 1105 66-67 0.12854891986020434 1105 68-69 0.11038702629746311 1105 70-71 0.020149332870349923 1105 72-73 0.014139463778601424 1105 74-75 -0.08029849812584544 1105 76-77 -0.222238379877588 1105 78-79 -0.43382492296490227 1105 80-81 -0.5431841128542167 1105 82-83 -0.5831531803993748 1105 84-85 -0.6561102605484912 1105 86-87 -0.5703856851681586 1105 88-89 -0.7298721490104079 1105 90-91 -1.187354576723255 1105 92-93 -1.592973756222925 1105 94-95 -1.4751575429493116 1105 96-97 -1.093361897315841 1105 98-99 -1.1558329558312792 1105 100-101 -1.6589076846763575 1106 1 0.2352712778561319 1106 2 0.35570277659494565 1106 3 0.3184888905120644 1106 4 0.37980695948850496 1106 5 0.33675167188700783 1106 6 0.35553576277436605 1106 7 0.44600937613461866 1106 8 0.23651400160859026 1106 9 0.4421061692087065 1106 10-11 0.40121879541302263 1106 12-13 0.46880293729815037 1106 14-15 0.5290048263295475 1106 16-17 0.08294532396401166 1106 18-19 0.4840479224441907 1106 20-21 0.6826335125497351 1106 22-23 0.5241596465103342 1106 24-25 0.303548534104074 1106 26-27 0.4149349384998189 1106 28-29 0.3397713466943344 1106 30-31 0.48433805639340477 1106 32-33 0.5531957395449112 1106 34-35 0.649318415605002 1106 36-37 0.48073659851639405 1106 38-39 0.569160974510659 1106 40-41 0.6497362871912387 1106 42-43 0.4538584133815533 1106 44-45 0.56231936905575 1106 46-47 0.5656234739450596 1106 48-49 0.5273163628184818 1106 50-51 0.5267571980820946 1106 52-53 0.4503187081458293 1106 54-55 0.48264727661789664 1106 56-57 0.5318601697797192 1106 58-59 0.4539193204312113 1106 60-61 0.4773132480470679 1106 62-63 0.598253072379265 1106 64-65 0.39175624400008857 1106 66-67 0.3048724871821342 1106 68-69 0.01452064499736494 1106 70-71 -0.044165457631020644 1106 72-73 0.041893161254062505 1106 74-75 -0.0783632811333419 1106 76-77 -0.6396145661572099 1106 78-79 -0.5394477235140549 1106 80-81 -0.7415078840094118 1106 82-83 -0.46391611631779384 1106 84-85 -0.05182736395790144 1106 86-87 -0.24946819567456657 1106 88-89 -0.35891719016674273 1106 90-91 -0.6096029775313916 1106 92-93 -0.26947957446360604 1106 94-95 -0.9633579949795497 1106 96-97 -0.9871749582320071 1106 98-99 -0.9959341530519517 1106 100-101 -1.0679183139815862 1107 1 0.0903168559786316 1107 2 -0.02044991149408304 1107 3 0.2039077515277441 1107 4 -0.01040573841650172 1107 5 0.0535221048378105 1107 6 0.13758906684358863 1107 7 0.069288684825068 1107 8 0.11180586151583327 1107 9 0.08893059003843717 1107 10-11 0.11685156754446524 1107 12-13 0.07332325030652953 1107 14-15 0.05260232711538748 1107 16-17 0.16229647678996884 1107 18-19 0.19724875845670198 1107 20-21 0.17632261074812305 1107 22-23 0.2134833918022494 1107 24-25 0.3492700360589964 1107 26-27 0.13096145266956682 1107 28-29 -0.014747036206010478 1107 30-31 -0.29926319431660175 1107 32-33 -0.21702159061937465 1107 34-35 -0.0859452095790516 1107 36-37 0.01883582812755691 1107 38-39 -0.060504234464893614 1107 40-41 -0.05166883145525958 1107 42-43 -0.18203442702056805 1107 44-45 -0.3991810417815316 1107 46-47 -0.19931969815991835 1107 48-49 -0.31870789036756975 1107 50-51 -0.3412901391960119 1107 52-53 -0.48559233041772387 1107 54-55 -0.31794885103393256 1107 56-57 -0.43883858447340884 1107 58-59 -0.591699058608917 1107 60-61 -0.5663673025300326 1107 62-63 -0.3966685376617072 1107 64-65 -0.529262632456458 1107 66-67 -0.5031959158464794 1107 68-69 -0.49715264837353956 1107 70-71 -0.7636623060403345 1107 72-73 -0.7506759228269537 1107 74-75 -0.8953598124534814 1107 76-77 -0.8788630372359201 1107 78-79 -0.9998264658556586 1107 80-81 -0.8648031850235469 1107 82-83 -0.8664313228117777 1107 84-85 -1.107137642610354 1107 86-87 -1.079108588975437 1107 88-89 -1.5596655206232946 1107 90-91 -1.5200614057617052 1107 92-93 -1.5576595928823522 1107 94-95 -1.2953961339142452 1107 96-97 -1.434892191890885 1107 98-99 -1.9575134225887805 1107 100-101 -2.00911178249601 1108 1 0.11144571964893046 1108 2 0.2207860854096637 1108 3 0.1854040125058063 1108 4 0.1948603011036809 1108 5 0.3170060116823592 1108 6 0.3277883154349084 1108 7 0.26094938724973105 1108 8 0.131615726629569 1108 9 0.2746570862050177 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4835.0 13 6199.0 14 7018.0 15 8845.0 16 10822.0 17 13753.0 18 13212.0 19 10889.0 20 10848.0 21 11588.0 22 12606.0 23 14310.0 24 16119.0 25 17844.0 26 20207.0 27 23158.0 28 26118.0 29 30101.0 30 35681.0 31 41868.0 32 50658.0 33 62391.0 34 80463.0 35 106150.0 36 127662.0 37 135657.0 38 68249.0 39 3759.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.83078649527112 18.548024283843965 14.424421337835582 22.196767883049333 2 15.512236215318604 20.249754548429717 40.47354981555779 23.76445942069389 3 19.841819540403055 24.7730781792058 29.551757993199168 25.833344287191974 4 12.722659428750498 16.836242146709328 36.79459380228156 33.64650462225862 5 13.62327783285116 37.508142992979835 33.52343765805499 15.345141516114023 6 31.392562305335343 35.898717223757004 17.385305277681958 15.323415193225696 7 28.354515631889132 33.33390637451005 20.31275913259094 17.998818861009873 8 26.519686980346357 34.14181253169698 19.337833714875707 20.000666773080955 9 26.612985558461094 17.246555389220045 20.404245834676875 35.73621321764199 10-11 25.242805769027683 26.67915076468469 26.394418334848368 21.68362513143926 12-13 26.56962041954959 24.290378387341725 27.203029687172968 21.936971505935716 14-15 23.093545580869414 25.41673006223971 26.516754881290634 24.972969475600244 16-17 21.833750347511817 28.87815603912351 26.884878813152373 22.403214800212297 18-19 21.570857405694472 27.04262513836869 29.908562936903877 21.47795451903296 20-21 22.625356355769423 25.752087587698902 30.110697749651226 21.511858306880445 22-23 22.25300750833909 25.828737036165965 30.10629016228027 21.811965293214676 24-25 22.158312832583874 26.070376884968667 30.1479645702532 21.62334571219427 26-27 21.766721787094944 26.240323382166565 30.29043629394573 21.70251853679276 28-29 21.422242664247317 26.799208489280996 30.205332841376904 21.573216005094782 30-31 21.673940207903527 26.155088371378575 30.073958195959662 22.097013224758236 32-33 21.52525068736859 26.350173863820153 30.13439066796054 21.99018478085072 34-35 22.2847453413764 26.216527155957092 29.846618218968572 21.652109283697936 36-37 22.060747094584457 25.99967362545034 30.009230438114887 21.93034884185031 38-39 21.638958276305463 26.465410050171055 29.97021251236095 21.92541916116253 40-41 21.872503038023016 26.390798351032828 29.88399449266783 21.85270411827632 42-43 21.74717237108674 26.43169469840729 29.487246185169603 22.33388674533636 44-45 21.878724220817258 26.399448562829498 29.474114771648452 22.24771244470479 46-47 22.22188553157813 26.88447857814194 28.990882585703343 21.902753304576585 48-49 22.13143604818221 26.815581802747456 28.84630243006757 22.206679719002764 50-51 21.88567849982071 27.1359524855684 28.868956530961654 22.109412483649237 52-53 22.342482263523323 27.178477604147993 28.39901379585089 22.08002633647779 54-55 21.853574184581696 26.93001523164627 28.678725389982667 22.53768519378937 56-57 22.146962430076137 27.16183963319224 28.47824363673268 22.21295429999894 58-59 22.489960042179945 26.72260020766754 28.42560532663195 22.36183442352057 60-61 22.073268522651755 26.865343790839113 29.048513077605172 22.012874608903964 62-63 21.801825825389034 27.034457959350235 28.933211315349823 22.230504899910912 64-65 21.711926674999052 27.226451539597775 28.641846254844776 22.419775530558397 66-67 21.80550071048908 27.091852553569463 28.42703676061416 22.675609975327294 68-69 21.813475035800245 26.90647990766441 28.647015888490124 22.63302916804522 70-71 21.980059908152953 26.99861769840977 28.455615088854668 22.565707304582606 72-73 22.07961495532896 26.861360686454244 28.500628778351846 22.55839557986495 74-75 21.787388162622417 27.563870460792465 28.109062684480858 22.539678692104257 76-77 22.062357021286694 27.07647628755944 28.04561383844611 22.815552852707754 78-79 22.239309956236102 27.2690782923396 27.99612042241148 22.49549132901282 80-81 22.262099093126576 27.33717706424659 27.98391954521517 22.416804297411666 82-83 22.27654336446182 27.178775406339874 28.11126684331276 22.43341438588555 84-85 22.270354326335664 27.261569375864607 27.949471388325104 22.518604909474618 86-87 22.267624094711458 27.080396489116342 27.969982590993364 22.681996825178835 88-89 22.236623708033505 27.124755143872864 27.690841294840617 22.94777985325301 90-91 22.281864407293572 27.04738841683857 27.794544070813277 22.876203105054582 92-93 22.62916357654838 26.817494751008873 27.747106739926885 22.80623493251586 94-95 22.390751410046246 26.716745514752127 27.80290420273346 23.089598872468166 96-97 22.304338667955744 26.663969300538614 27.94006593898396 23.09162609252168 98-99 22.4867142304456 26.724271164046552 27.32822946981168 23.46078513569617 100-101 23.327562720662755 25.615808614471685 27.432869241286582 23.623759423578978 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1288.0 1 857.0 2 704.5 3 911.0 4 636.0 5 501.0 6 914.5 7 1898.0 8 3038.0 9 3463.5 10 2703.0 11 1850.5 12 1666.0 13 1692.5 14 1817.0 15 1895.0 16 1922.5 17 1960.0 18 2100.0 19 2196.0 20 2285.5 21 2606.5 22 3081.5 23 3592.5 24 4172.5 25 5027.0 26 6063.5 27 7119.0 28 8141.0 29 9403.0 30 10993.5 31 12598.5 32 14405.5 33 15784.0 34 16954.5 35 18282.0 36 19827.0 37 21762.0 38 23321.0 39 24495.0 40 25755.5 41 27790.5 42 29689.0 43 31519.5 44 33683.5 45 35285.5 46 35973.5 47 37667.5 48 41660.5 49 42267.5 50 39327.5 51 36508.5 52 34413.5 53 33600.0 54 32524.5 55 30268.0 56 27497.0 57 25093.5 58 23166.0 59 20861.5 60 17899.0 61 14762.5 62 11450.5 63 8678.0 64 6719.0 65 5371.5 66 4271.0 67 3370.5 68 2669.5 69 1949.0 70 1379.5 71 1010.0 72 812.0 73 652.0 74 488.5 75 407.0 76 298.0 77 180.0 78 113.5 79 71.0 80 39.0 81 18.0 82 11.5 83 12.5 84 11.0 85 9.5 86 7.0 87 4.5 88 2.0 89 1.5 90 1.5 91 0.5 92 1.5 93 2.5 94 1.0 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1393214640868639 2 0.05078166408383518 3 0.1532536104955503 4 0.12922570581969983 5 0.19535292246962438 6 0.19747303170572886 7 0.16546947799881878 8 0.06794445313801406 9 0.12276442052871486 10-11 0.09989752805358827 12-13 0.15163828917280406 14-15 0.1368480033114087 16-17 0.13528316077999827 18-19 0.13225443329984907 20-21 0.13477837286664007 22-23 0.1966148922530199 24-25 0.21569587537795995 26-27 0.1482562101533041 28-29 0.12796373603630434 30-31 0.09787837640015547 32-33 0.12468261459947602 34-35 0.09343624276260329 36-37 0.08626825439291681 38-39 0.05068070650116353 40-41 0.05673816146146197 42-43 0.06012024048096192 44-45 0.03785909350186519 46-47 0.04911586396975311 48-49 0.04023159669464874 50-51 0.050478791335820256 52-53 0.025592747207260867 54-55 0.04755102143834268 56-57 0.024381256215201184 58-59 0.04755102143834268 60-61 0.03523419635240254 62-63 0.05017591858780533 64-65 0.040635427025335305 66-67 0.03553706910041746 68-69 0.06537003477988723 70-71 0.05042831254448443 72-73 0.050478791335820256 74-75 0.04093829977335023 76-77 0.020342952908335564 78-79 0.02029247411699974 80-81 0.024684128963216107 82-83 0.021302049943716147 84-85 0.018677152794253495 86-87 0.022513540935775834 88-89 0.06542051357122305 90-91 0.11064951060811799 92-93 0.08086702371998404 94-95 0.07496100513369308 96-97 0.06668248335461856 98-99 0.05512284013871572 100-101 0.13200203934316995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 990515.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.45643861962593 #Duplication Level Percentage of deduplicated Percentage of total 1 84.66271675263484 54.57057205736982 2 9.1145139357946 11.749782161005395 3 2.495044261167892 4.824650018196546 4 1.1150153538369696 2.874796748581325 5 0.6317997023722383 2.036177936792705 6 0.38320593490804516 1.482005389324607 7 0.2823425904388064 1.2739158495227574 8 0.19974898974609687 1.0300086797521266 9 0.15794507943284797 0.9162519585961775 >10 0.8673779041659441 10.263052900457179 >50 0.059103311341852295 2.538224845123288 >100 0.028208609285449583 3.2515948009844857 >500 0.001723859137840444 0.7752714308426776 >1k 9.402868024584241E-4 1.1710386576884901 >5k 3.13428934152808E-4 1.242656565762426 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6755 0.6819684709469316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5547 0.5600117110795899 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3356 0.3388136474460256 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2840 0.28671953478745904 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1659 0.1674886296522516 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1526 0.15406127115692342 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1150 0.11610122007238657 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1062 0.10721695279728222 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0095758267164051E-4 0.0 10-11 0.0 0.0 0.0 1.0095758267164051E-4 0.0 12-13 0.0 0.0 0.0 1.0095758267164051E-4 0.0 14-15 0.0 0.0 0.0 1.0095758267164051E-4 5.0478791335820256E-5 16-17 0.0 0.0 0.0 3.028727480149215E-4 1.0095758267164051E-4 18-19 0.0 0.0 0.0 4.0383033068656205E-4 1.0095758267164051E-4 20-21 0.0 0.0 0.0 6.05745496029843E-4 1.0095758267164051E-4 22-23 0.0 0.0 0.0 8.581394527089443E-4 1.0095758267164051E-4 24-25 0.0 0.0 0.0 0.0013124485747313267 1.0095758267164051E-4 26-27 0.0 0.0 0.0 0.002372503192783552 1.0095758267164051E-4 28-29 0.0 0.0 0.0 0.0055526670469402285 1.0095758267164051E-4 30-31 0.0 0.0 0.0 0.015093158609410257 1.0095758267164051E-4 32-33 0.0 0.0 0.0 0.032912171950954805 1.0095758267164051E-4 34-35 0.0 0.0 0.0 0.06148316784702908 1.0095758267164051E-4 36-37 0.0 0.0 0.0 0.10398631015178972 1.0095758267164051E-4 38-39 0.0 0.0 0.0 0.17001256921904262 1.0095758267164051E-4 40-41 0.0 0.0 0.0 0.24270202874262378 1.0095758267164051E-4 42-43 0.0 0.0 0.0 0.30787014835716775 1.0095758267164051E-4 44-45 0.0 0.0 0.0 0.3915135056006219 1.5143637400746076E-4 46-47 0.0 0.0 0.0 0.49100720332352366 2.0191516534328103E-4 48-49 0.0 0.0 0.0 0.5892894100543656 2.0191516534328103E-4 50-51 0.0 0.0 0.0 0.695900617355618 2.0191516534328103E-4 52-53 0.0 0.0 0.0 0.7956971878265346 2.0191516534328103E-4 54-55 0.0 0.0 0.0 0.9080629773400706 2.0191516534328103E-4 56-57 0.0 0.0 0.0 1.040216453057248 2.0191516534328103E-4 58-59 0.0 0.0 0.0 1.1841314871556716 2.0191516534328103E-4 60-61 0.0 0.0 0.0 1.33788988556458 2.0191516534328103E-4 62-63 5.0478791335820256E-5 0.0 0.0 1.4871556715446004 2.0191516534328103E-4 64-65 1.0095758267164051E-4 0.0 0.0 1.636724330272636 2.0191516534328103E-4 66-67 1.0095758267164051E-4 0.0 0.0 1.8031024265154996 2.0191516534328103E-4 68-69 1.0095758267164051E-4 0.0 0.0 1.9907320939107433 2.0191516534328103E-4 70-71 1.0095758267164051E-4 0.0 0.0 2.1869936346244128 2.0191516534328103E-4 72-73 1.0095758267164051E-4 0.0 0.0 2.3847695390781567 2.0191516534328103E-4 74-75 1.0095758267164051E-4 0.0 0.0 2.574418358126833 2.0191516534328103E-4 76-77 1.0095758267164051E-4 0.0 0.0 2.7804727843596515 2.523939566791013E-4 78-79 1.0095758267164051E-4 0.0 0.0 3.020549915952812 3.028727480149215E-4 80-81 1.0095758267164051E-4 0.0 0.0 3.2706218482304656 3.028727480149215E-4 82-83 1.0095758267164051E-4 0.0 0.0 3.5288208659131866 3.028727480149215E-4 84-85 1.0095758267164051E-4 0.0 0.0 3.77273438564787 3.028727480149215E-4 86-87 1.0095758267164051E-4 0.0 0.0 4.025835045405673 3.028727480149215E-4 88-89 1.0095758267164051E-4 0.0 0.0 4.311545004366415 3.028727480149215E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAACG 65 0.005825505 29.249413 8 GGTATCA 6105 0.0 28.08878 1 GTAAGCG 145 0.0 26.205057 94-95 GTATCAA 8205 0.0 24.441032 1 GTACATG 4810 0.0 23.866463 1 TACATGG 4790 0.0 23.233494 2 GTATAGG 230 3.5088306E-9 22.781427 1 GAGTACT 4925 0.0 22.438162 12-13 CGCTCCG 120 5.973561E-9 21.758308 48-49 ACATGGG 4995 0.0 21.327831 3 TACCTGG 670 0.0 21.295145 2 GTACTTT 5190 0.0 21.201427 14-15 ATTCGCG 135 1.0131771E-9 21.094702 80-81 ATCAACG 9525 0.0 20.821177 3 TATCAAC 9630 0.0 20.742313 2 TCAACGC 9645 0.0 20.611433 4 CAACGCA 9650 0.0 20.551472 5 TACACCG 140 4.8105267E-4 20.382498 5 CAATTCG 140 1.5952537E-9 20.33824 78-79 AACGCAG 9885 0.0 20.058832 6 >>END_MODULE