FastQCFastQC Report
Thu 26 May 2016
SRR938264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990515
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66360.6699545186090065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58910.5947411195186343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35310.3564812244135626No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29100.2937865655744739No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16290.16445990217210238No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15590.15739287138508756No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11810.11923090513520744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.1148897290803269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGC601.5046317E-731.67175378-79
GGTATCA59400.027.8341561
ATTCGCG605.3775366E-627.71278480-81
GTATCAA82250.027.2063481
CGCGGAA806.236951E-826.7230482-83
GGACCGA1658.42756E-825.9027846
TACCTGG8000.024.9377232
GTACCTG9400.024.2604391
GAGTACT49800.023.46169712-13
ATCAACG95200.023.2976263
TATCAAC95900.023.1806032
TCAACGC96150.023.065114
GTACATG51850.022.9990671
CAACGCA96500.022.9814535
AACGCAG97800.022.6274196
TACATGG52000.022.380012
GTACTTT53550.021.68349314-15
ACATGGG51950.021.6667883
AGTACTT50700.020.18798412-13
ACGCAGA110550.020.0197647
Produced by FastQC (version 0.11.5)