##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938264_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 990515 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47156681120427 33.0 31.0 34.0 31.0 34.0 2 32.68761906684907 34.0 31.0 34.0 31.0 34.0 3 32.73354164247891 34.0 31.0 34.0 31.0 34.0 4 36.20270869194308 37.0 35.0 37.0 35.0 37.0 5 36.180281974528405 37.0 35.0 37.0 35.0 37.0 6 36.21812491481704 37.0 37.0 37.0 35.0 37.0 7 36.17846978591945 37.0 37.0 37.0 35.0 37.0 8 36.17571667263999 37.0 37.0 37.0 35.0 37.0 9 37.84449099710756 39.0 38.0 39.0 35.0 39.0 10-11 37.822031468478514 39.0 38.0 39.0 35.0 39.0 12-13 37.734359398898555 39.0 38.0 39.0 35.0 39.0 14-15 38.9673922151608 40.0 38.0 41.0 35.5 41.0 16-17 38.85493051594372 40.0 38.0 41.0 35.0 41.0 18-19 39.12690014790286 40.0 38.5 41.0 36.0 41.0 20-21 39.12536660222207 40.0 39.0 41.0 36.0 41.0 22-23 39.017939152864926 40.0 38.5 41.0 35.0 41.0 24-25 38.905081195135864 40.0 38.0 41.0 35.0 41.0 26-27 38.723080417762475 40.0 38.0 41.0 35.0 41.0 28-29 38.59732411927129 40.0 38.0 41.0 34.5 41.0 30-31 38.34280500547695 40.0 38.0 41.0 34.0 41.0 32-33 38.10893474606644 40.0 37.0 41.0 34.0 41.0 34-35 37.93379908431473 40.0 37.0 41.0 33.0 41.0 36-37 37.9204358338844 40.0 37.0 41.0 33.0 41.0 38-39 37.76678243136146 40.0 37.0 41.0 33.0 41.0 40-41 37.58860491764385 40.0 37.0 41.0 33.0 41.0 42-43 37.32843167443198 40.0 36.5 41.0 32.5 41.0 44-45 37.01345158831517 40.0 36.0 41.0 31.5 41.0 46-47 36.555461552828575 39.5 36.0 41.0 30.5 41.0 48-49 36.10975401684982 39.0 35.0 41.0 29.5 41.0 50-51 35.70313725688152 39.0 35.0 40.0 28.0 41.0 52-53 35.17644053850775 38.0 34.5 40.0 27.0 41.0 54-55 34.85694663886968 38.0 34.0 40.0 26.0 41.0 56-57 34.49189563005103 38.0 34.0 40.0 25.0 41.0 58-59 34.04467019681681 37.5 33.5 40.0 23.5 41.0 60-61 33.53814631782457 37.0 33.0 40.0 22.5 41.0 62-63 33.079439988288925 36.0 32.0 39.0 21.5 40.5 64-65 32.58041826726501 36.0 31.5 39.0 19.5 40.0 66-67 31.996161592706827 35.0 31.0 38.5 17.5 40.0 68-69 31.40308627330227 35.0 30.5 38.0 12.0 40.0 70-71 30.766569410862026 34.5 29.5 37.0 8.0 39.5 72-73 30.156158664936928 34.0 29.0 36.5 7.0 39.0 74-75 29.53409135651656 34.0 28.5 36.0 2.0 39.0 76-77 28.195251460099037 32.5 27.0 34.5 2.0 36.5 78-79 28.885734188780585 34.0 28.5 35.0 2.0 37.0 80-81 28.52420508523344 34.0 27.5 35.0 2.0 37.0 82-83 27.93570162996017 33.0 26.5 35.0 2.0 36.0 84-85 27.314784733194347 33.0 26.0 35.0 2.0 36.0 86-87 26.631915720609985 32.5 25.0 35.0 2.0 35.5 88-89 25.855029454374744 32.0 23.5 34.0 2.0 35.0 90-91 25.269828826418582 31.5 20.0 34.0 2.0 35.0 92-93 24.405438080190606 31.0 16.0 34.0 2.0 35.0 94-95 23.531575998344294 31.0 4.5 34.0 2.0 35.0 96-97 22.42297138357319 30.0 2.0 34.0 2.0 35.0 98-99 20.92464980338511 29.0 2.0 34.0 2.0 35.0 100-101 16.707377980141644 14.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18835590800981095 1101 2 0.05884554337263381 1101 3 0.017378491137577612 1101 4 0.011129127220158352 1101 5 0.05058650768606299 1101 6 0.0647669211293902 1101 7 0.054091101119695395 1101 8 0.053805326195508485 1101 9 -0.053643104547489884 1101 10-11 0.06786253010802312 1101 12-13 0.16943907772490974 1101 14-15 0.08577914053309854 1101 16-17 0.1709244149217355 1101 18-19 0.27508676332336535 1101 20-21 0.2714108229486527 1101 22-23 0.10355773966352189 1101 24-25 0.2324824411746036 1101 26-27 0.287288264896425 1101 28-29 0.06263946791285946 1101 30-31 0.18255097635627493 1101 32-33 0.19841995341769803 1101 34-35 0.14847039016902386 1101 36-37 0.23464897229879966 1101 38-39 0.21529297041295337 1101 40-41 0.20992036071609022 1101 42-43 0.3336939751070034 1101 44-45 0.10769551387964071 1101 46-47 -0.044526819397532336 1101 48-49 0.12781731617941006 1101 50-51 0.1815682114343531 1101 52-53 0.05438559323919634 1101 54-55 0.030555644685847483 1101 56-57 0.10424749235140496 1101 58-59 0.07658201956467536 1101 60-61 0.2271145598690154 1101 62-63 0.13598904652720023 1101 64-65 0.1455198066257708 1101 66-67 0.017241585263853665 1101 68-69 0.21809227667540654 1101 70-71 0.20504228440757544 1101 72-73 0.003432808240585672 1101 74-75 -0.02815731203085292 1101 76-77 -0.14680078297297783 1101 78-79 -0.06474108296293224 1101 80-81 -0.2840795001090264 1101 82-83 -0.4261785259527002 1101 84-85 -0.37735794158684044 1101 86-87 -0.4412862548523009 1101 88-89 -0.5457844097988058 1101 90-91 -0.770769113858421 1101 92-93 -0.5383853792637048 1101 94-95 -0.9183328884067592 1101 96-97 -1.1922905855222865 1101 98-99 -1.3705503718358507 1101 100-101 -1.36836080068524 1102 1 0.20174547352073802 1102 2 0.16617971174936486 1102 3 0.18522665537462046 1102 4 0.14824947118696485 1102 5 0.18215025969593768 1102 6 0.02292813780538694 1102 7 0.15319698284556438 1102 8 0.20218320998233708 1102 9 0.2864105514323967 1102 10-11 0.28343031519983697 1102 12-13 0.23883762439407263 1102 14-15 0.24828280495225385 1102 16-17 0.2767850426256331 1102 18-19 0.1949814501376821 1102 20-21 0.15944060912423907 1102 22-23 0.22586383636865293 1102 24-25 0.370729871639476 1102 26-27 0.12335214571618991 1102 28-29 0.09057233575391876 1102 30-31 0.22505712813873657 1102 32-33 0.04206696103080532 1102 34-35 0.14668037693269298 1102 36-37 0.1756840263545314 1102 38-39 0.2077220583501358 1102 40-41 0.13717164659408354 1102 42-43 0.2607265062656765 1102 44-45 -0.015672153950760048 1102 46-47 -0.03180129105491858 1102 48-49 0.07123868440690018 1102 50-51 0.31970681975457893 1102 52-53 0.26247463196211385 1102 54-55 0.42991292777443846 1102 56-57 0.34378835173542655 1102 58-59 0.3838191363552639 1102 60-61 0.03272933836043279 1102 62-63 0.1274631646236628 1102 64-65 0.08383541749554269 1102 66-67 0.22931262613656145 1102 68-69 0.3041472740547917 1102 70-71 0.09438904061932263 1102 72-73 0.2379478612303032 1102 74-75 0.0018742685996890884 1102 76-77 0.06832505095998798 1102 78-79 -0.30589206591560725 1102 80-81 -0.24594622185302129 1102 82-83 -0.19672148182865357 1102 84-85 -0.19352624724825418 1102 86-87 -0.2443481546648627 1102 88-89 -0.5405731615866003 1102 90-91 -0.6548235604645001 1102 92-93 -0.6488565125241941 1102 94-95 -0.7902247947042333 1102 96-97 -0.57366001227388 1102 98-99 -0.6420205368013399 1102 100-101 -1.33293141959453 1103 1 0.14839819318601144 1103 2 0.1818700883183979 1103 3 0.0952684963788073 1103 4 -0.004470194085001822 1103 5 0.08173184965409774 1103 6 0.08255156876772674 1103 7 0.1082179033476578 1103 8 0.062015845965603944 1103 9 0.09812603260394326 1103 10-11 0.11815834867263675 1103 12-13 -0.03155986514568099 1103 14-15 0.06993552461752728 1103 16-17 0.06914905936203297 1103 18-19 0.1411111572506485 1103 20-21 0.23161634134181952 1103 22-23 0.30379270666153246 1103 24-25 0.32575079214156943 1103 26-27 0.24102996998396975 1103 28-29 0.3295890721555921 1103 30-31 0.3019399733270163 1103 32-33 0.27846863467097194 1103 34-35 0.11373058613772002 1103 36-37 0.2802865368147849 1103 38-39 0.21242917132084216 1103 40-41 0.15076997295391692 1103 42-43 0.023278807520902944 1103 44-45 -0.09621608700514628 1103 46-47 -0.13797283917207182 1103 48-49 -0.2064809808650665 1103 50-51 -0.46062790827888733 1103 52-53 -0.4969395939793131 1103 54-55 -0.5763632228531748 1103 56-57 -0.17294804993820634 1103 58-59 -0.1951557590422226 1103 60-61 -0.477730912685594 1103 62-63 -0.6099845341211108 1103 64-65 -0.626938641081864 1103 66-67 -0.5980932316040253 1103 68-69 -0.5206435209242883 1103 70-71 -0.6587490346944875 1103 72-73 -0.9022195027864335 1103 74-75 -0.9144437230321074 1103 76-77 -1.0221561205881287 1103 78-79 -0.766143111940714 1103 80-81 -0.719272581685658 1103 82-83 -0.9273700173935069 1103 84-85 -1.2081705987126874 1103 86-87 -1.491210079351056 1103 88-89 -1.6488367599129603 1103 90-91 -1.6150746064896069 1103 92-93 -1.378982035536744 1103 94-95 -1.3074842089301733 1103 96-97 -1.65263490767137 1103 98-99 -1.5949494070942265 1103 100-101 -1.7267598714773769 1104 1 0.09872804404722046 1104 2 0.11537583396958695 1104 3 0.025460759643429753 1104 4 -0.0077772591396296775 1104 5 -0.032535162692845176 1104 6 0.04709794896469788 1104 7 0.02917348685426191 1104 8 0.02609376093167981 1104 9 0.038329333679492095 1104 10-11 0.05106300423304333 1104 12-13 0.04567486374343588 1104 14-15 0.17354762684990277 1104 16-17 0.11390567135303087 1104 18-19 0.04415596734040861 1104 20-21 0.135669742099914 1104 22-23 -0.054981069553264206 1104 24-25 0.09626593276670548 1104 26-27 0.04334833809924277 1104 28-29 -0.1939090582617098 1104 30-31 -0.12515485772602375 1104 32-33 -0.05822951970436918 1104 34-35 0.04456993037196355 1104 36-37 0.01727111810527049 1104 38-39 0.03317490807332746 1104 40-41 0.12587714239715098 1104 42-43 -0.14083483059291524 1104 44-45 -1.6467134172160058E-4 1104 46-47 -0.26728742533972394 1104 48-49 -0.26045395106297775 1104 50-51 -0.21105679996288274 1104 52-53 -0.25969618030098474 1104 54-55 -0.16545076381714097 1104 56-57 -0.13976081786181282 1104 58-59 -0.33841413793861364 1104 60-61 -0.550124834188793 1104 62-63 -0.4878370381640309 1104 64-65 -0.5041975419212505 1104 66-67 -0.5233195495100915 1104 68-69 -0.5195889393771118 1104 70-71 -0.7146365089182041 1104 72-73 -1.0767548879546958 1104 74-75 -1.0958927137997065 1104 76-77 -0.9000310852928024 1104 78-79 -0.9739181373961294 1104 80-81 -0.8854237226872748 1104 82-83 -0.8409467387358411 1104 84-85 -0.9628274425832792 1104 86-87 -1.1422195256636556 1104 88-89 -1.3311809684848015 1104 90-91 -0.8269053857248316 1104 92-93 -0.8844250025687721 1104 94-95 -1.163953093426752 1104 96-97 -1.2748358813945337 1104 98-99 -1.3359255688452158 1104 100-101 -1.4766475681686018 1105 1 -0.01586908053554481 1105 2 0.04629060948689556 1105 3 0.03169518135886307 1105 4 -0.01862067756691488 1105 5 0.021846647464968783 1105 6 0.0010887869132432115 1105 7 -0.01020655180785468 1105 8 0.05671577347511203 1105 9 0.05368851220274706 1105 10-11 0.034010027187896696 1105 12-13 0.14342413183599945 1105 14-15 0.15459573072792665 1105 16-17 0.13196249596252585 1105 18-19 -0.013097530586627215 1105 20-21 0.08618440821959439 1105 22-23 0.0974141791087888 1105 24-25 0.12873403030463493 1105 26-27 0.2343839482707395 1105 28-29 0.04806948488509022 1105 30-31 0.023310467576528993 1105 32-33 0.1438313202953907 1105 34-35 0.41453593008603207 1105 36-37 0.2780943352657772 1105 38-39 0.33255301984538477 1105 40-41 0.46663988007281887 1105 42-43 0.2922943145516328 1105 44-45 0.08567517301528227 1105 46-47 0.11801133585642987 1105 48-49 0.26405173947848226 1105 50-51 0.21248606712521223 1105 52-53 0.17701480483709986 1105 54-55 0.3574372714387266 1105 56-57 0.29682585178841947 1105 58-59 0.11119924089418731 1105 60-61 -0.10664953256834053 1105 62-63 0.10698460018979006 1105 64-65 -0.08108045537902342 1105 66-67 -0.24905627416993426 1105 68-69 -0.13101135718903478 1105 70-71 -0.27905834866310997 1105 72-73 -0.35057373542580805 1105 74-75 -0.4141061759238873 1105 76-77 -0.009211162492722025 1105 78-79 -0.2649375865542041 1105 80-81 -0.2723199379022496 1105 82-83 -0.13899933661874897 1105 84-85 -0.2563969131857782 1105 86-87 -0.6771179909889575 1105 88-89 -0.563537492872392 1105 90-91 -0.6466572520110923 1105 92-93 -0.5384192591932333 1105 94-95 -0.6731664250196694 1105 96-97 -1.038325665769321 1105 98-99 -1.5228261351022638 1105 100-101 -1.7638831646653905 1106 1 0.1099416063226002 1106 2 0.1416593978180387 1106 3 0.19371864616464762 1106 4 0.1060197665497924 1106 5 0.15276995220169454 1106 6 0.09044689198329792 1106 7 0.0997135335623085 1106 8 0.0701723659824367 1106 9 0.07840629305800917 1106 10-11 0.02825713305529831 1106 12-13 0.06314440065610682 1106 14-15 0.1230327492467751 1106 16-17 0.08992967880696057 1106 18-19 0.2232123679163962 1106 20-21 0.35177615417140373 1106 22-23 0.28688918855106493 1106 24-25 0.342206809571195 1106 26-27 0.39680848499968846 1106 28-29 0.28679240953272966 1106 30-31 0.2589093476925939 1106 32-33 0.2800704766654718 1106 34-35 0.38443755056049156 1106 36-37 0.48189642515625053 1106 38-39 0.3716806134737922 1106 40-41 0.31703225910677446 1106 42-43 0.27286720592649516 1106 44-45 0.14253326309759018 1106 46-47 0.1410552859052956 1106 48-49 0.2412703746349223 1106 50-51 0.2752817002972776 1106 52-53 0.1895493515778668 1106 54-55 0.030073854713613457 1106 56-57 -0.08942139796128856 1106 58-59 -0.09406145585587211 1106 60-61 -0.061623363974248946 1106 62-63 -0.10326521546100764 1106 64-65 -0.2775049801177829 1106 66-67 -0.04748975558213786 1106 68-69 -0.2955215878203781 1106 70-71 -0.6653637508441257 1106 72-73 -0.6812290593708177 1106 74-75 -0.5553668247131753 1106 76-77 -0.509146415455799 1106 78-79 -0.5624238427965977 1106 80-81 -0.6934772312363009 1106 82-83 -0.6244888062326055 1106 84-85 -0.8213208054419106 1106 86-87 -0.9502857397704574 1106 88-89 -0.8454251088025657 1106 90-91 -1.0600221195494441 1106 92-93 -0.9906077689922199 1106 94-95 -1.0212255126859624 1106 96-97 -0.9391925566525785 1106 98-99 -0.9739762942720631 1106 100-101 -1.77714671508115 1107 1 -0.02607255515574991 1107 2 -0.04479565548051312 1107 3 -0.015797337887143215 1107 4 0.023937235130397028 1107 5 0.027122125227606375 1107 6 -0.009239327596823443 1107 7 -0.019958678948100328 1107 8 0.017941436828429858 1107 9 0.04134821886465545 1107 10-11 -0.0012795858713374741 1107 12-13 -0.007554905056458949 1107 14-15 0.024223350172121627 1107 16-17 -0.038564230323551385 1107 18-19 -0.05316972729859515 1107 20-21 -0.05325977341094301 1107 22-23 -0.002445034470163421 1107 24-25 -0.04727024144431624 1107 26-27 0.019733756731326935 1107 28-29 0.03702374676672093 1107 30-31 -0.04802415181384845 1107 32-33 -0.09781379405698942 1107 34-35 0.05076874606939441 1107 36-37 -0.0511674283320076 1107 38-39 -0.036081729567058574 1107 40-41 -0.20438918273907092 1107 42-43 -0.11252849379281571 1107 44-45 -0.27827661023216876 1107 46-47 -0.15275513336931823 1107 48-49 -0.3135474507399394 1107 50-51 -0.5219714802450213 1107 52-53 -0.48743714318050735 1107 54-55 -0.48759049398522336 1107 56-57 -0.5166326124404037 1107 58-59 -0.3128369953210708 1107 60-61 -0.4597798350750182 1107 62-63 -0.41851417182402884 1107 64-65 -0.8255630157089797 1107 66-67 -1.063777661525215 1107 68-69 -1.1455621200890285 1107 70-71 -1.05123315422016 1107 72-73 -1.1023968298754596 1107 74-75 -1.1555681074078272 1107 76-77 -1.041821490040686 1107 78-79 -1.200327855766922 1107 80-81 -1.049845760549129 1107 82-83 -1.1465697935655825 1107 84-85 -1.4589485372340967 1107 86-87 -1.5522075759097973 1107 88-89 -1.5413818082075394 1107 90-91 -1.7805176609998945 1107 92-93 -1.528597258175342 1107 94-95 -1.8311777011527361 1107 96-97 -2.0227513449859025 1107 98-99 -2.327302973328184 1107 100-101 -2.0946599638941326 1108 1 0.025718519592075495 1108 2 -0.027575730278115884 1108 3 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54-55 0.37050184777318407 2316 56-57 0.4995300857776499 2316 58-59 0.35019100221237665 2316 60-61 0.6305425167037768 2316 62-63 0.5359359679709428 2316 64-65 0.8600489639138971 2316 66-67 0.8581565717747139 2316 68-69 0.9243064586271039 2316 70-71 0.8534237875284312 2316 72-73 1.1772351484861012 2316 74-75 1.3867623517783478 2316 76-77 1.1442542830359947 2316 78-79 1.1790880360128533 2316 80-81 1.256908718846006 2316 82-83 1.3237566224278048 2316 84-85 1.6306078327215445 2316 86-87 1.893990631631425 2316 88-89 2.076986689925022 2316 90-91 2.140076870529299 2316 92-93 2.3028827991441005 2316 94-95 2.666085961265189 2316 96-97 3.0870542734404047 2316 98-99 3.045392949844146 2316 100-101 3.130847011839702 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 9.0 5 26.0 6 54.0 7 123.0 8 259.0 9 451.0 10 868.0 11 1410.0 12 2144.0 13 3350.0 14 4356.0 15 6200.0 16 8581.0 17 11979.0 18 12194.0 19 9196.0 20 8162.0 21 8781.0 22 9499.0 23 10355.0 24 11628.0 25 13221.0 26 15416.0 27 17745.0 28 21377.0 29 25637.0 30 30544.0 31 37371.0 32 45976.0 33 59102.0 34 79097.0 35 111512.0 36 149083.0 37 178902.0 38 92057.0 39 3845.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97401141922489 19.041633221766194 14.933034474750992 22.051320884257926 2 15.822087593083427 20.538684841600404 39.89084942572258 23.748378139593587 3 19.932574672187506 24.678295970735913 29.861201560132024 25.527927796944557 4 13.027983451016341 17.27971538009559 36.44683467371486 33.24546649517322 5 13.516135708552707 37.6981438761036 33.60750324329509 15.178217172048603 6 31.388075041343004 36.17730142736856 17.204849297421287 15.229774233867142 7 28.252171849997225 33.325997082325856 20.18465141870643 18.237179648970486 8 26.392432219602934 34.5615159790614 19.18961348389474 19.856438317440926 9 26.37173591515525 17.229622973907514 20.391715420765966 36.00692569017128 10-11 25.23858800724875 26.798584574690942 26.299803637501707 21.6630237805586 12-13 26.482333551235097 24.289871606222064 27.2252355484436 22.002559294099235 14-15 22.93943049827615 25.50350070417914 26.421356567038355 25.135712230506353 16-17 21.730698073328316 29.013604875564177 26.896774880728287 22.358922170379223 18-19 21.517190552389415 27.089493849159275 29.898638586997674 21.49467701145364 20-21 22.51384380852385 25.755036521405533 30.12220915382402 21.6089105162466 22-23 22.19380827145475 25.9322675577856 30.097323109695463 21.776601061064195 24-25 22.155201352869167 26.061661555547143 30.245964739585308 21.537172351998386 26-27 21.7571502211622 26.258095102018352 30.335800003332203 21.648954673487246 28-29 21.31861408310776 26.854285278696832 30.34623690871514 21.480863729480266 30-31 21.651197751395536 26.129476486676673 30.162870362985256 22.056455398942536 32-33 21.535766747600995 26.30157039519846 30.163551283928058 21.99911157327249 34-35 22.221773485159034 26.25505586439884 29.96418741682939 21.55898323361274 36-37 22.132431035387903 25.95395494029326 30.089105792179367 21.82450823213947 38-39 21.598587773967104 26.4575822343015 30.073054529411113 21.87077546232028 40-41 21.883318113693488 26.45678202800313 29.86547655645426 21.794423301849122 42-43 21.76758146733583 26.32563309637967 29.59492735406451 22.31185808221999 44-45 21.767413921041072 26.42024603362897 29.522167761215123 22.290172284114828 46-47 22.210225090092067 26.825654748113227 29.029862763240722 21.93425739855398 48-49 22.10254207300175 26.81193182707301 28.91867375459422 22.166852345331016 50-51 21.87553162543483 27.108618932402738 28.920827500702945 22.09502194145949 52-53 22.333222607798376 27.158769310920334 28.393022473918077 22.114985607363213 54-55 21.907625950271623 26.94154772548174 28.66037889201825 22.490447432228386 56-57 22.1447244461812 27.10867157199821 28.49020195869949 22.256402023121097 58-59 22.503626530703954 26.770890609292504 28.417358269929004 22.30812459007454 60-61 22.07649550494131 26.927998796538837 28.97206546546031 22.023440233059542 62-63 21.80328282700784 27.076203795800286 28.89983388955928 22.220679487632598 64-65 21.619228132109594 27.233542596730885 28.57934825744548 22.56788101371404 66-67 21.76672857902307 27.175923144535147 28.35567411853145 22.701674157910332 68-69 21.701076712006426 27.104784382399146 28.541982578099873 22.65215632749456 70-71 21.890264682494603 27.202696050588816 28.295505320156007 22.611533946760574 72-73 21.997513292978613 26.930087993889746 28.46173999827354 22.6106587148581 74-75 21.702046019665016 27.68606661870322 27.9347192245068 22.67716813712497 76-77 21.801234772352732 27.09940583901509 28.239813229069057 22.859546159563124 78-79 21.972226962931245 27.27825852494494 28.222523607889393 22.526990904234424 80-81 21.96991994571838 27.482240590958817 28.078318602611795 22.469520860711004 82-83 21.87227312390925 27.36238365328679 28.28406777196044 22.481275450843512 84-85 21.79463940845163 27.405614054575334 28.159338370616467 22.640408166356572 86-87 21.936748215359646 27.324627557159744 28.006923955157898 22.731700272322712 88-89 21.75549162308476 27.334564633674685 27.79584784520757 23.114095898032982 90-91 21.815972951574594 27.260347153775307 28.044058718027436 22.879621176622663 92-93 22.092110883674337 27.080028234923446 27.960854843612253 22.86700603778996 94-95 21.788664514585115 27.034388539913305 28.02832104317727 23.14862590232431 96-97 21.662016800104205 27.021934647412888 28.136791905092235 23.179256647390673 98-99 21.769410144451225 27.039534896635566 27.64267291058644 23.54838204832677 100-101 22.060208124982015 26.426056969077088 27.722180965646203 23.791553940294694 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1308.0 1 861.0 2 685.5 3 905.5 4 628.0 5 461.0 6 883.0 7 1911.5 8 3051.0 9 3474.0 10 2693.5 11 1771.5 12 1631.0 13 1714.5 14 1813.0 15 1859.5 16 1939.0 17 2049.5 18 2107.0 19 2180.5 20 2346.0 21 2644.5 22 3014.5 23 3525.0 24 4170.0 25 4951.0 26 5963.0 27 7229.0 28 8258.0 29 9372.5 30 11140.5 31 12674.0 32 14286.5 33 15814.5 34 16986.0 35 18159.0 36 19710.5 37 21786.0 38 23660.5 39 24915.0 40 26234.0 41 28261.0 42 29986.0 43 31764.5 44 33697.0 45 35279.0 46 35942.5 47 38294.5 48 43182.5 49 43394.0 50 39490.5 51 36506.5 52 34644.5 53 33894.5 54 32763.5 55 30259.5 56 27104.0 57 24511.5 58 22842.0 59 20833.0 60 17738.0 61 14068.5 62 10946.5 63 8246.0 64 6124.5 65 4882.0 66 3836.0 67 3090.0 68 2356.0 69 1648.5 70 1193.5 71 885.5 72 704.5 73 588.0 74 485.5 75 395.5 76 260.5 77 140.5 78 72.0 79 36.5 80 21.5 81 12.5 82 6.5 83 5.0 84 4.0 85 1.0 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00807660661373124 2 0.014134061574029673 3 0.008682352109761085 4 1.009575826716405E-4 5 0.001009575826716405 6 0.002120109236104451 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.571818700373038E-4 14-15 0.0 16-17 0.0024734607754551925 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002776333523470114 26-27 0.01802092850688783 28-29 0.006663200456328273 30-31 0.0034325578108357776 32-33 0.0 34-35 0.008177564196402882 36-37 7.067030787014836E-4 38-39 0.002221066818776091 40-41 0.0018677152794253494 42-43 0.0026248971494626534 44-45 0.0 46-47 5.047879133582025E-5 48-49 4.543091220223823E-4 50-51 0.0038868669328581593 52-53 0.00787469144838796 54-55 0.007521339909037219 56-57 0.016557043558149045 58-59 0.02458317138054447 60-61 0.0038868669328581593 62-63 0.021201092361044508 64-65 0.021806837857074348 66-67 0.01378071003467893 68-69 0.03276073557694734 70-71 0.01791997092421619 72-73 0.004896442759574565 74-75 0.01791997092421619 76-77 0.021302049943716147 78-79 0.027662377652029498 80-81 0.012569219042619243 82-83 0.03745526317117863 84-85 0.023472637971156417 86-87 0.033265523490305544 88-89 0.02362407434516388 90-91 0.02937865655744739 92-93 0.025441310833253405 94-95 0.01574938289677592 96-97 0.016557043558149045 98-99 0.011256770467887917 100-101 0.01014623705849987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 990515.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.546603645802065 #Duplication Level Percentage of deduplicated Percentage of total 1 83.3842171795263 52.15399582243312 2 9.900713176681972 12.385119657453943 3 2.698933247923745 5.064273243730912 4 1.1814984978613032 2.955948730153658 5 0.6746446369992496 2.1098365356079034 6 0.44360003440393125 1.664740531747611 7 0.2931499905434631 1.2834875387104827 8 0.21299475698094605 1.0657678914816933 9 0.16500417766871964 0.928840581049222 >10 0.9482443744376506 10.981805694107862 >50 0.06338069236043051 2.661249532876578 >100 0.030386616207692996 3.430242822190742 >500 0.001939571042749628 0.8403998341352562 >1k 9.69785521374814E-4 1.2078788906830313 >5k 3.232618404582713E-4 1.2664126936379592 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6636 0.6699545186090065 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5891 0.5947411195186343 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3531 0.3564812244135626 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2910 0.2937865655744739 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1629 0.16445990217210238 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1559 0.15739287138508756 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1181 0.11923090513520744 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1138 0.1148897290803269 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.0478791335820256E-5 0.0 14-15 0.0 0.0 0.0 1.0095758267164051E-4 0.0 16-17 0.0 0.0 0.0 2.523939566791013E-4 0.0 18-19 0.0 0.0 0.0 4.0383033068656205E-4 0.0 20-21 0.0 0.0 0.0 6.05745496029843E-4 0.0 22-23 0.0 0.0 0.0 9.086182440447646E-4 0.0 24-25 0.0 0.0 0.0 0.0013124485747313267 0.0 26-27 0.0 0.0 0.0 0.002422981984119372 0.0 28-29 0.0 0.0 0.0 0.005653624629611869 0.0 30-31 0.0 0.0 0.0 0.015295073774753538 0.0 32-33 0.0 0.0 0.0 0.03326552349030555 0.0 34-35 0.0 0.0 0.0 0.06183651938637982 0.0 36-37 0.0 0.0 0.0 0.10474349202182703 0.0 38-39 0.0 0.0 0.0 0.1706687935064083 0.0 40-41 0.0 0.0 0.0 0.2437116045693402 0.0 42-43 0.0 0.0 0.0 0.3097883424279289 0.0 44-45 0.0 0.0 0.0 0.393734572419398 0.0 46-47 0.0 0.0 0.0 0.4929253973942848 0.0 48-49 0.0 0.0 0.0 0.5916114344558134 0.0 50-51 0.0 0.0 0.0 0.6983740781310732 0.0 52-53 0.0 0.0 0.0 0.7984735213500047 0.0 54-55 0.0 0.0 0.0 0.9111421836115556 0.0 56-57 0.0 0.0 0.0 1.0436490108680836 0.0 58-59 0.0 0.0 0.0 1.1882202692538728 0.0 60-61 0.0 0.0 0.0 1.3444016496469007 0.0 62-63 0.0 0.0 0.0 1.4944246174969587 0.0 64-65 0.0 0.0 0.0 1.6444475853470164 0.0 66-67 0.0 0.0 0.0 1.8125924392866337 0.0 68-69 0.0 0.0 0.0 2.0008783309692433 0.0 70-71 0.0 0.0 0.0 2.1959283806908525 0.0 72-73 0.0 0.0 0.0 2.394410988223298 0.0 74-75 0.0 0.0 0.0 2.585725607386057 0.0 76-77 0.0 0.0 0.0 2.794707803516353 0.0 78-79 0.0 0.0 0.0 3.038368929294357 0.0 80-81 0.0 0.0 0.0 3.2919238981741823 0.0 82-83 0.0 0.0 0.0 3.5534545160850666 1.0095758267164051E-4 84-85 0.0 0.0 0.0 3.8026178301186757 1.0095758267164051E-4 86-87 0.0 0.0 0.0 4.05980727197468 1.0095758267164051E-4 88-89 0.0 0.0 0.0 4.353543358757818 1.0095758267164051E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATTCGC 60 1.5046317E-7 31.671753 78-79 GGTATCA 5940 0.0 27.834156 1 ATTCGCG 60 5.3775366E-6 27.712784 80-81 GTATCAA 8225 0.0 27.206348 1 CGCGGAA 80 6.236951E-8 26.72304 82-83 GGACCGA 165 8.42756E-8 25.902784 6 TACCTGG 800 0.0 24.937723 2 GTACCTG 940 0.0 24.260439 1 GAGTACT 4980 0.0 23.461697 12-13 ATCAACG 9520 0.0 23.297626 3 TATCAAC 9590 0.0 23.180603 2 TCAACGC 9615 0.0 23.06511 4 GTACATG 5185 0.0 22.999067 1 CAACGCA 9650 0.0 22.981453 5 AACGCAG 9780 0.0 22.627419 6 TACATGG 5200 0.0 22.38001 2 GTACTTT 5355 0.0 21.683493 14-15 ACATGGG 5195 0.0 21.666788 3 AGTACTT 5070 0.0 20.187984 12-13 ACGCAGA 11055 0.0 20.019764 7 >>END_MODULE