FastQCFastQC Report
Thu 26 May 2016
SRR938263_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938263_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575381
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124740.4843555186591809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99430.3860787976613946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61900.24035278663622975No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39750.15434609481082606No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37090.14401752595052927No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34760.13497032089620914No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33780.13116505868452086No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC30870.11986575966818112No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28910.11225523524480456No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27400.10639202510230525No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA27040.10499417367760343No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26350.10231495844692494No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA25840.10033466892859735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53150.056.5320431
GTATCAA102100.044.003031
ATCAACG128850.034.4500273
TATCAAC129250.034.343412
TCAACGC130800.033.9364364
CAACGCA132750.033.3663335
AACGCAG137000.032.3249666
TGGTATC23150.028.741152
ACGCAGA154950.028.3891537
CGCAGAG155400.028.3375158
TAAGGTG17250.025.3469585
GTGGTAT27650.025.1834451
GCAGAGT170950.025.1475479
TACATGG96150.024.6154162
GTACATG101150.024.37711
GAGTACT107450.023.29718612-13
TAGACCG2303.608875E-922.7296075
GGACCGA6150.022.4060656
ACATGGG101500.022.2410183
CAGAGTA161150.022.01902210-11
Produced by FastQC (version 0.11.5)