FastQCFastQC Report
Thu 26 May 2016
SRR938263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575381
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120500.46789193521269284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99040.3845644586179676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63040.2447793161477855No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40330.15659818877284565No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37690.1463472783250323No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35190.136639976764603No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35110.13632934311466924No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC34700.13473734565875886No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA29150.11318713619460577No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28510.11070206699513588No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA27580.10709095081465617No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26690.10363515145914333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.055.5386351
GTATCAA101750.041.2752271
ATCAACG129900.032.0321543
TATCAAC130350.031.9271562
TCAACGC131750.031.5811374
CAACGCA133850.031.1554995
AACGCAG138550.030.0984216
TGGTATC25050.028.1007042
ACGCAGA158450.026.2583587
CGCAGAG159300.026.0586198
TAAGGTG17650.024.2466245
GTAAGGT17450.023.9804634
GTACATG99550.023.3153421
GTGGTAT30300.023.2317711
GCAGAGT175700.023.2207979
TACATGG94500.023.2023682
GAGTACT111050.022.17613812-13
ACATGGG96700.021.8345243
CAGAGTA166900.021.21519510-11
GTACTTT115700.021.0796214-15
Produced by FastQC (version 0.11.5)