##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938263_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2575381 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.484925143114744 33.0 31.0 34.0 31.0 34.0 2 32.711990187082996 34.0 31.0 34.0 31.0 34.0 3 32.77282895229871 34.0 31.0 34.0 31.0 34.0 4 36.232404836410616 37.0 35.0 37.0 35.0 37.0 5 36.15882737350318 37.0 35.0 37.0 35.0 37.0 6 36.21115982450752 37.0 37.0 37.0 35.0 37.0 7 36.18428263623907 37.0 37.0 37.0 35.0 37.0 8 36.17730968738218 37.0 37.0 37.0 35.0 37.0 9 37.87396427946001 39.0 38.0 39.0 35.0 39.0 10-11 37.84749091493647 39.0 38.0 39.0 35.0 39.0 12-13 37.76542558169063 39.0 38.0 39.0 35.0 39.0 14-15 39.01064560932926 40.0 38.0 41.0 36.0 41.0 16-17 38.923117977495366 40.0 38.0 41.0 35.5 41.0 18-19 39.17580039613556 40.0 39.0 41.0 36.0 41.0 20-21 39.15026961059354 40.0 39.0 41.0 36.0 41.0 22-23 39.044773181133195 40.0 39.0 41.0 35.5 41.0 24-25 38.931569154233884 40.0 38.0 41.0 35.0 41.0 26-27 38.77060462121915 40.0 38.0 41.0 35.0 41.0 28-29 38.64617138202075 40.0 38.0 41.0 34.5 41.0 30-31 38.39638542802017 40.0 38.0 41.0 34.0 41.0 32-33 38.15960123958358 40.0 38.0 41.0 34.0 41.0 34-35 38.06004237819569 40.0 38.0 41.0 34.0 41.0 36-37 37.99226541626268 40.0 38.0 41.0 33.0 41.0 38-39 37.79657747727424 40.0 37.0 41.0 33.0 41.0 40-41 37.571805686226625 40.0 37.0 41.0 33.0 41.0 42-43 37.29790368881342 40.0 37.0 41.0 32.0 41.0 44-45 37.011753600729364 40.0 36.0 41.0 31.0 41.0 46-47 36.62786321713176 39.0 36.0 41.0 30.5 41.0 48-49 36.19116666621366 39.0 35.0 41.0 30.0 41.0 50-51 35.79710613691722 39.0 35.0 40.0 28.5 41.0 52-53 35.26474082863856 38.0 35.0 40.0 27.0 41.0 54-55 34.88032935709319 38.0 34.0 40.0 26.0 41.0 56-57 34.497527162000495 38.0 34.0 40.0 25.5 41.0 58-59 33.98936603943261 37.5 33.0 40.0 23.5 41.0 60-61 33.50897420614659 37.0 33.0 39.5 22.5 41.0 62-63 32.95223133975128 36.0 32.0 39.0 21.0 40.5 64-65 32.38099624871039 35.5 31.0 39.0 18.5 40.0 66-67 31.812782069915094 35.0 31.0 38.0 15.0 40.0 68-69 31.191135991140726 35.0 30.0 38.0 9.5 40.0 70-71 30.566029841798166 34.5 29.5 37.0 7.5 39.5 72-73 29.898035086847344 34.0 29.0 36.5 4.0 39.0 74-75 29.245469116996667 34.0 28.0 36.0 2.0 39.0 76-77 27.947089382114726 32.0 26.5 34.5 2.0 36.5 78-79 28.60688224383111 33.5 28.0 35.0 2.0 37.0 80-81 28.333002184919437 34.0 27.5 35.0 2.0 37.0 82-83 27.778169909617255 33.0 26.0 35.0 2.0 36.0 84-85 27.12728077903813 33.0 25.5 35.0 2.0 36.0 86-87 26.42748762221978 32.5 24.5 35.0 2.0 35.5 88-89 25.618135141945988 32.0 21.5 34.5 2.0 35.0 90-91 24.963267182603275 31.5 19.0 34.0 2.0 35.0 92-93 24.022970775974507 31.0 10.0 34.0 2.0 35.0 94-95 23.12612095064769 30.5 2.0 34.0 2.0 35.0 96-97 22.07929603425668 30.0 2.0 34.0 2.0 35.0 98-99 20.663937491190623 29.0 2.0 34.0 2.0 35.0 100-101 16.80040914334617 21.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1793326029843385 1101 2 0.157200619637635 1101 3 0.08221272138512603 1101 4 0.044568237284217105 1101 5 -0.1052792787390544 1101 6 -0.011565005459310385 1101 7 0.012241633752488212 1101 8 0.07671913388485763 1101 9 -0.07386697563338629 1101 10-11 0.07679848967150349 1101 12-13 0.25317358790452715 1101 14-15 0.2806885074015284 1101 16-17 0.29393947423163524 1101 18-19 0.26502721901129433 1101 20-21 0.15069159270800725 1101 22-23 0.2240483509307225 1101 24-25 0.30039680232576416 1101 26-27 0.3595280837518544 1101 28-29 0.3485624217971335 1101 30-31 0.2683576902450895 1101 32-33 0.3440589000592027 1101 34-35 0.3269962130351587 1101 36-37 0.3272008699627591 1101 38-39 0.2881903203453433 1101 40-41 0.4967559106408572 1101 42-43 0.4601727974317811 1101 44-45 0.43415659791524064 1101 46-47 0.48005001400502323 1101 48-49 0.4772869881217616 1101 50-51 0.5674931335213671 1101 52-53 0.5414039428728756 1101 54-55 0.7754853471432028 1101 56-57 0.7067476197157845 1101 58-59 0.9991360502644469 1101 60-61 0.8694404623040697 1101 62-63 0.8339906163695687 1101 64-65 0.8898134370524318 1101 66-67 1.1363568714025938 1101 68-69 1.382399330636165 1101 70-71 1.5752683719895408 1101 72-73 1.6960271407840075 1101 74-75 1.673356174333005 1101 76-77 1.6657736585889573 1101 78-79 1.7727519270600958 1101 80-81 1.8589363861613286 1101 82-83 2.038425085506315 1101 84-85 2.184152048259339 1101 86-87 2.3417886834392228 1101 88-89 2.7112434804284753 1101 90-91 2.9826105945775723 1101 92-93 3.2424631951183365 1101 94-95 3.47994341190946 1101 96-97 3.769749903768478 1101 98-99 4.1984661906714 1101 100-101 4.083740662732296 1102 1 0.22413665558708118 1102 2 0.22256687977282752 1102 3 0.22022793204214253 1102 4 0.1286653707840557 1102 5 0.014045248769136265 1102 6 0.0529362719197124 1102 7 0.11209176890324812 1102 8 0.19772941766797203 1102 9 0.2113841975859998 1102 10-11 0.21380258148202813 1102 12-13 0.27000324078163374 1102 14-15 0.309429088936092 1102 16-17 0.3570682557402094 1102 18-19 0.4013660511104078 1102 20-21 0.23437454370586153 1102 22-23 0.30531910750260494 1102 24-25 0.3444251837630645 1102 26-27 0.3305652279958764 1102 28-29 0.36375622125063956 1102 30-31 0.45370670431211835 1102 32-33 0.3618007591960719 1102 34-35 0.5028193644750232 1102 36-37 0.579504662445494 1102 38-39 0.6044585159073605 1102 40-41 0.7927489237951164 1102 42-43 0.7805563594548772 1102 44-45 0.5752787355345745 1102 46-47 0.5267938027188066 1102 48-49 0.6720066859270659 1102 50-51 0.8069271257807316 1102 52-53 0.7246080274491646 1102 54-55 0.7502543762056462 1102 56-57 0.9756236244153556 1102 58-59 0.9441491366452865 1102 60-61 0.9904801072726386 1102 62-63 1.2802858822747325 1102 64-65 1.2437246818729122 1102 66-67 1.2362624913790974 1102 68-69 1.461842660422338 1102 70-71 1.486269371832801 1102 72-73 1.782355612738602 1102 74-75 1.6730441419629116 1102 76-77 1.6627988011414203 1102 78-79 1.9241380876039322 1102 80-81 1.993185215398018 1102 82-83 2.224874530010549 1102 84-85 2.253272521110624 1102 86-87 2.393783100571685 1102 88-89 2.60594455676711 1102 90-91 2.782285478059439 1102 92-93 2.8538268223478376 1102 94-95 3.132759227741815 1102 96-97 3.452329811965093 1102 98-99 3.623894138322303 1102 100-101 3.341685486924664 1103 1 0.2611002283023254 1103 2 0.23919437869896143 1103 3 0.21817794880382024 1103 4 0.09569716481518498 1103 5 0.0636285217289938 1103 6 0.06131882571355618 1103 7 0.09790907432846296 1103 8 0.15535152284900278 1103 9 0.1975062997662178 1103 10-11 0.2612233407560325 1103 12-13 0.29008617464974407 1103 14-15 0.4053468272498577 1103 16-17 0.31180679499640007 1103 18-19 0.3061194229513049 1103 20-21 0.36832350075224696 1103 22-23 0.4028986525966616 1103 24-25 0.4249986989398806 1103 26-27 0.4217470420467109 1103 28-29 0.3974156856828728 1103 30-31 0.4405976573785537 1103 32-33 0.38485256778542265 1103 34-35 0.4919583543674264 1103 36-37 0.5354409543507614 1103 38-39 0.5308376676378401 1103 40-41 0.6296788016792121 1103 42-43 0.6550939129014921 1103 44-45 0.7040510776511439 1103 46-47 0.8137445148114821 1103 48-49 0.9216920293614166 1103 50-51 1.018908982014402 1103 52-53 1.0579755128802262 1103 54-55 1.1699727971612575 1103 56-57 1.17301060312073 1103 58-59 1.3594445751539297 1103 60-61 1.3507164340730569 1103 62-63 1.4333015696621558 1103 64-65 1.382562390247152 1103 66-67 1.6678077551976251 1103 68-69 1.6623817777206433 1103 70-71 1.796084556112664 1103 72-73 1.8895133208384856 1103 74-75 1.8452742425072586 1103 76-77 1.8633130545694208 1103 78-79 1.9825043084071012 1103 80-81 2.093320594884979 1103 82-83 2.2640576318383836 1103 84-85 2.437757294562182 1103 86-87 2.5557499956144305 1103 88-89 2.782743179137224 1103 90-91 2.999504394149021 1103 92-93 3.036518610668846 1103 94-95 3.088140583783005 1103 96-97 3.4084930938587945 1103 98-99 3.8272045054416353 1103 100-101 3.6085899179235668 1104 1 0.22757757273744517 1104 2 0.19652444015023462 1104 3 0.19906629156741928 1104 4 0.0668558923522724 1104 5 0.060142644401842915 1104 6 0.0848706141228277 1104 7 0.15127485323942835 1104 8 0.19283505218008656 1104 9 0.1885781522604404 1104 10-11 0.20193542555010424 1104 12-13 0.25546855185575623 1104 14-15 0.36297759127381113 1104 16-17 0.3028318782983135 1104 18-19 0.3152389081939546 1104 20-21 0.28886457059599735 1104 22-23 0.3787908079117699 1104 24-25 0.3992677299430909 1104 26-27 0.4063063098744948 1104 28-29 0.3921095635651639 1104 30-31 0.42899047720171524 1104 32-33 0.44753190886938654 1104 34-35 0.5028424889182332 1104 36-37 0.5566828924985572 1104 38-39 0.6164826097272353 1104 40-41 0.636260677795434 1104 42-43 0.6616468753087901 1104 44-45 0.6430615145869965 1104 46-47 0.6955195040677538 1104 48-49 0.8021382054270347 1104 50-51 0.8463621123850018 1104 52-53 0.8461602757411413 1104 54-55 0.9333568008394906 1104 56-57 0.9192469512848049 1104 58-59 0.9523195676972378 1104 60-61 1.1351742705809542 1104 62-63 1.1997576295494454 1104 64-65 1.3126139398393377 1104 66-67 1.365133369010298 1104 68-69 1.4607180930549752 1104 70-71 1.6129569936986208 1104 72-73 1.8540719109695125 1104 74-75 1.924340960738398 1104 76-77 1.9047654723439074 1104 78-79 1.853362800048913 1104 80-81 1.9385505836563155 1104 82-83 2.1666442015119003 1104 84-85 2.444107759555493 1104 86-87 2.4254571129318556 1104 88-89 2.558526395713276 1104 90-91 2.7777846392074146 1104 92-93 3.051622929301569 1104 94-95 3.2919042153286107 1104 96-97 3.760990974534657 1104 98-99 4.202019812785231 1104 100-101 3.7909541584223163 1105 1 0.18585322129071358 1105 2 0.1681342143487825 1105 3 0.1494421678296476 1105 4 0.06885933630129415 1105 5 0.034621320530554556 1105 6 0.03154472951523957 1105 7 0.12305693665721407 1105 8 0.09838761897272974 1105 9 0.08885973346670539 1105 10-11 0.19940806843449366 1105 12-13 0.22224724124762218 1105 14-15 0.37505991372639613 1105 16-17 0.2698420208846599 1105 18-19 0.21331269603119551 1105 20-21 0.22085374920446554 1105 22-23 0.294288410857682 1105 24-25 0.19322818935485486 1105 26-27 0.27359877878804895 1105 28-29 0.2862646671965834 1105 30-31 0.2789994000250644 1105 32-33 0.4429036832408073 1105 34-35 0.4501705410826631 1105 36-37 0.5260330874221992 1105 38-39 0.5628549511356908 1105 40-41 0.638908930784865 1105 42-43 0.5498509773486404 1105 44-45 0.7418167714152801 1105 46-47 0.78251665919597 1105 48-49 0.9221595866726346 1105 50-51 0.8810912683715983 1105 52-53 1.0109328760046168 1105 54-55 1.0089697187033124 1105 56-57 1.0336967837816218 1105 58-59 1.140852305331208 1105 60-61 1.0322292918019897 1105 62-63 1.2162423128432351 1105 64-65 1.3089841762811218 1105 66-67 1.4238353012579417 1105 68-69 1.45559557281749 1105 70-71 1.6592073960639233 1105 72-73 1.7824357036053335 1105 74-75 1.7230572477411066 1105 76-77 1.7944317811041408 1105 78-79 1.935467304752116 1105 80-81 2.099757035062506 1105 82-83 2.0737503454811907 1105 84-85 2.2161030779605753 1105 86-87 2.206596183050717 1105 88-89 2.4870824295536913 1105 90-91 2.7369176425515143 1105 92-93 2.8657894854419617 1105 94-95 3.1710645050018336 1105 96-97 3.485196191275442 1105 98-99 3.5866154803539914 1105 100-101 3.36657918450155 1106 1 0.19648282943213502 1106 2 0.11443618085925067 1106 3 0.12174925048749685 1106 4 0.1419035827868953 1106 5 -0.00918690583467452 1106 6 0.01802045904509697 1106 7 0.12899581771164037 1106 8 0.12604687745059806 1106 9 0.1614206109012457 1106 10-11 0.19561832321475237 1106 12-13 0.18704405968684767 1106 14-15 0.1045817912941871 1106 16-17 0.14423893731629533 1106 18-19 0.08992901551419408 1106 20-21 0.0653853635529984 1106 22-23 -0.008043315640797744 1106 24-25 0.04127993101023719 1106 26-27 -0.026371795730597114 1106 28-29 0.07303246544882569 1106 30-31 0.10114578763083415 1106 32-33 0.21024268282996417 1106 34-35 0.228987213393836 1106 36-37 0.3203073329084347 1106 38-39 0.026560632474378565 1106 40-41 0.09351544131141054 1106 42-43 0.13019329840840044 1106 44-45 0.16402020559103647 1106 46-47 0.3921137383005444 1106 48-49 0.4020071224273707 1106 50-51 0.5604836460615346 1106 52-53 0.5654253627909185 1106 54-55 0.6112230929177116 1106 56-57 0.5483193048236075 1106 58-59 0.6514718505132038 1106 60-61 0.9409773347298866 1106 62-63 0.8469157399679759 1106 64-65 0.9306004194244295 1106 66-67 1.130816843708363 1106 68-69 1.1066652307046319 1106 70-71 1.0057993146053121 1106 72-73 1.317522987633403 1106 74-75 1.5108674297904479 1106 76-77 1.4886935693406151 1106 78-79 1.7550717541069751 1106 80-81 1.6890409895696017 1106 82-83 2.001858481349185 1106 84-85 2.111737870261095 1106 86-87 2.3799818414597453 1106 88-89 2.533220925023276 1106 90-91 2.372075506095012 1106 92-93 2.499230052909599 1106 94-95 2.9045888379317226 1106 96-97 3.2481583240121203 1106 98-99 3.634356935860442 1106 100-101 3.391692340745305 1107 1 0.21584729815979387 1107 2 0.18768054639511433 1107 3 0.19742501268749635 1107 4 0.11396836578532543 1107 5 0.04646209799633283 1107 6 0.13762910122461136 1107 7 0.11602047649574843 1107 8 0.1760574153925205 1107 9 0.16957884543581514 1107 10-11 0.28042607914001394 1107 12-13 0.23473791780079978 1107 14-15 0.45602582281730264 1107 16-17 0.36861022902787255 1107 18-19 0.3373145387830405 1107 20-21 0.22018739697561074 1107 22-23 0.3354075535393193 1107 24-25 0.3582970731737447 1107 26-27 0.37629380335807383 1107 28-29 0.38869918369360335 1107 30-31 0.3808909628541315 1107 32-33 0.4494986507732719 1107 34-35 0.48320022254984707 1107 36-37 0.4385078592719722 1107 38-39 0.4086177187734563 1107 40-41 0.395317429380853 1107 42-43 0.5938795838772464 1107 44-45 0.668701752987765 1107 46-47 0.7754198708491842 1107 48-49 0.947494682320233 1107 50-51 0.9429550802354143 1107 52-53 1.0698598021190477 1107 54-55 1.1001612552073539 1107 56-57 1.063285016494845 1107 58-59 1.1144011382550403 1107 60-61 1.1654119874846884 1107 62-63 1.121697077672998 1107 64-65 1.3585053898648312 1107 66-67 1.6174668386394835 1107 68-69 1.7617775664994788 1107 70-71 1.74940351751005 1107 72-73 1.875113507220636 1107 74-75 1.7526646757860327 1107 76-77 1.6460710624309236 1107 78-79 1.6528955690553815 1107 80-81 1.7917240629670346 1107 82-83 2.105027767383614 1107 84-85 2.103171457216458 1107 86-87 2.4334383864554248 1107 88-89 2.5847181131268755 1107 90-91 2.7981480084693864 1107 92-93 2.9752275558175363 1107 94-95 2.918643151017978 1107 96-97 3.339712030166279 1107 98-99 3.875401019139531 1107 100-101 3.8682766002614635 1108 1 0.1914312600261212 1108 2 0.21671766987581975 1108 3 0.14103319242391166 1108 4 0.10343026280733625 1108 5 0.007731587857065847 1108 6 0.05223451995302497 1108 7 0.12789938314192284 1108 8 0.1233036802250993 1108 9 0.16966692012314866 1108 10-11 0.18084963831819323 1108 12-13 0.23224864022674296 1108 14-15 0.36810476576092555 1108 16-17 0.3321160319487433 1108 18-19 0.2791185218940555 1108 20-21 0.22989512913247978 1108 22-23 0.2505158987493914 1108 24-25 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-0.27348411198777 2316 50-51 -0.15502547674885392 2316 52-53 -0.03143046083582135 2316 54-55 0.225194740698754 2316 56-57 0.3373590756864786 2316 58-59 0.3682218328244957 2316 60-61 0.47059808645172296 2316 62-63 0.43188456963648747 2316 64-65 0.6148508904863235 2316 66-67 0.7223802317645074 2316 68-69 0.9144251973340012 2316 70-71 1.089912121941925 2316 72-73 1.1370182691253987 2316 74-75 1.5380179879029576 2316 76-77 1.6074609514198244 2316 78-79 1.613331121539197 2316 80-81 1.6705688670903065 2316 82-83 1.841232414634053 2316 84-85 2.054909488162405 2316 86-87 2.1979838142124812 2316 88-89 2.5103963952408 2316 90-91 2.8052342081607797 2316 92-93 2.8785750334224396 2316 94-95 3.24167969574388 2316 96-97 3.692371849719663 2316 98-99 4.029305695321433 2316 100-101 4.743141988586014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 14.0 5 30.0 6 99.0 7 251.0 8 618.0 9 1214.0 10 2223.0 11 3613.0 12 5568.0 13 8367.0 14 11216.0 15 15931.0 16 20876.0 17 29012.0 18 30106.0 19 24529.0 20 23170.0 21 24062.0 22 26733.0 23 29472.0 24 32889.0 25 37103.0 26 41637.0 27 48463.0 28 58364.0 29 69827.0 30 84534.0 31 100379.0 32 123323.0 33 153787.0 34 200656.0 35 274380.0 36 379555.0 37 461628.0 38 241364.0 39 10375.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27565513607502 18.73528615765978 14.871585990577705 23.117472715687505 2 15.873186008146071 19.937483106691147 39.75314021368676 24.436190671476016 3 19.428693245690095 23.712639629959977 29.648788932038308 27.20987819231162 4 12.946398408905408 16.76900937897764 36.45663047977466 33.82796173234229 5 13.637740164915543 37.3733792524881 34.09287316213559 14.89600742046077 6 32.660319121962246 36.05508160158175 16.801371759263397 14.483227517192615 7 29.02296786378404 32.92242972981473 19.781810924286543 18.272791482114688 8 26.915578376862985 34.50947455725796 19.19071077526306 19.38423629061599 9 26.41205320688473 16.720322158158346 19.91546105217053 36.95216358278639 10-11 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29.238841230425226 21.914951826021074 36-37 22.45123843682649 25.97012033446929 29.285846444589282 22.292794784114943 38-39 21.872074252960065 26.405047846824925 29.206037441019078 22.516840459195937 40-41 22.459020754222774 26.194990552847266 29.28427066058616 22.061718032343805 42-43 22.117329370436433 26.206416110775756 29.020408155340483 22.655846363447324 44-45 22.041689779008795 26.573559765361754 29.24767312026035 22.137077335369096 46-47 22.61139225613608 26.87720768305738 28.3668319367115 22.144568124095038 48-49 22.305573427776316 26.89139975793873 28.29538619722674 22.507640617058215 50-51 22.48859489139665 27.06174348572114 28.271312089356876 22.178349533525328 52-53 22.854501916415472 26.728977188229624 28.1999440082846 22.216576887070303 54-55 22.223643802606293 27.013925318234467 28.399720274398234 22.362710604761006 56-57 22.411484438901358 27.145328622314256 28.061717482135162 22.38146945664922 58-59 22.324308519787948 26.70156376862297 28.109413713932035 22.864713997657045 60-61 21.948558290986846 27.078750678055012 28.388168585541322 22.584522445416813 62-63 22.133462971109903 27.011013127766336 28.528109044836476 22.327414856287284 64-65 21.832750183370926 27.052482720032494 28.314470752094543 22.800296344502037 66-67 21.786314682432362 27.07792110719173 28.27741376468448 22.85835044569142 68-69 22.42534424393695 26.666508759572473 28.388621525429127 22.519525471061456 70-71 22.1328999476194 26.880605238603533 27.95646547054591 23.030029343231156 72-73 22.10575179308525 27.018522901187964 27.945085374796218 22.930639930930568 74-75 22.17335998052327 27.33436334274424 27.713938248360147 22.778338428372347 76-77 22.19840481854918 26.908212804241394 28.123838764050834 22.769543613158596 78-79 22.26062862155153 27.089253978343397 28.00240042152986 22.647716978575207 80-81 22.44846926502705 27.18376954395671 27.6893841511963 22.678377039819942 82-83 22.35041767608776 27.291238645590198 27.747453618403302 22.610890059918745 84-85 22.251710018192302 27.409082172125977 27.678219523039377 22.660988286642343 86-87 22.349249543451073 27.393382282989275 27.472091590141574 22.78527658341808 88-89 22.152877574230764 27.5162005155742 27.201742188825655 23.12917972136938 90-91 22.17193067882517 27.329917054871306 27.474637102927524 23.023515163376004 92-93 22.292390912257254 27.032582751833612 27.58436518713154 23.090661148777595 94-95 21.97443134108973 26.845655174315088 27.7689365780849 23.410976906510285 96-97 22.26082283503014 26.511541128064188 27.731925798033803 23.49571023887187 98-99 21.875015774365036 26.78922070171365 27.40332401302953 23.932439510891786 100-101 22.243155478742757 26.143335685088925 27.67215802244406 23.94135081372426 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 941.0 1 633.0 2 498.0 3 693.0 4 579.5 5 546.5 6 1082.0 7 2370.0 8 3812.0 9 4302.5 10 3534.0 11 2717.5 12 2612.5 13 2755.5 14 2965.5 15 3189.0 16 3380.0 17 3613.5 18 3916.0 19 4366.5 20 5009.0 21 5723.5 22 6799.5 23 8361.0 24 10243.0 25 12873.5 26 16429.0 27 20258.0 28 23604.0 29 26936.0 30 30923.5 31 36441.5 32 44919.5 33 48906.5 34 49240.5 35 52335.0 36 57395.0 37 62307.0 38 65447.0 39 68466.5 40 71341.5 41 75193.5 42 77691.5 43 79792.5 44 82748.0 45 87859.5 46 91185.0 47 92956.5 48 93130.0 49 90869.0 50 90744.5 51 88855.5 52 87088.0 53 88684.5 54 91137.0 55 87156.5 56 79596.5 57 73098.0 58 67834.0 59 60030.0 60 48285.0 61 39644.0 62 33494.5 63 25248.0 64 17627.0 65 13350.0 66 10137.0 67 7672.0 68 5795.0 69 4464.0 70 3391.5 71 2383.5 72 1697.0 73 1357.5 74 1035.0 75 737.5 76 533.5 77 342.5 78 215.0 79 119.5 80 70.0 81 45.5 82 35.0 83 24.0 84 10.0 85 6.5 86 6.0 87 3.0 88 1.5 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015415194877961747 3 0.0035722869742379863 4 0.0018249726933607104 5 0.11780781173737012 6 7.765841248343449E-5 7 0.0 8 3.882920624171725E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.9414603120858622E-5 18-19 0.0 20-21 0.0 22-23 2.1356063432944483E-4 24-25 5.436088873840415E-4 26-27 0.005649649508169859 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011163396794493708 36-37 6.795111092300517E-4 38-39 0.007144573948475973 40-41 0.010347983463417646 42-43 0.0062320876017956175 44-45 0.003785847608567431 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.9414603120858622E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.9414603120858622E-5 68-69 0.0010483885685263657 70-71 0.0 72-73 5.8243809362575866E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.9414603120858622E-5 82-83 0.002329752374503035 84-85 0.004504187924039201 86-87 0.0031839949118208136 88-89 0.0 90-91 0.0024850691994699036 92-93 0.0 94-95 5.8243809362575866E-5 96-97 1.7473142808772758E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2575381.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.02109331942495 #Duplication Level Percentage of deduplicated Percentage of total 1 73.93134294629816 37.72057947693487 2 14.631401365091733 14.930201888846137 3 4.946577786778883 7.571394206131197 4 2.214000194937288 4.518428422204815 5 1.1535568871124267 2.942786679331423 6 0.69362719256458 2.1233770632436904 7 0.43339883452927647 1.5478737666733755 8 0.30311049111063765 1.2372022922442054 9 0.2413931202607581 1.1084526823942178 >10 1.2918047198051066 12.202569946533464 >50 0.10041907719473839 3.5207270904571244 >100 0.0521374444987719 4.911738576813297 >500 0.0035769175940207868 1.2743425419788317 >1k 0.0034247083346954574 3.2930837820335244 >5k 1.522092593197981E-4 0.6293471440436671 >10k+ 7.610462965989905E-5 0.4678944401361172 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12050 0.46789193521269284 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9904 0.3845644586179676 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6304 0.2447793161477855 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4033 0.15659818877284565 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3769 0.1463472783250323 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3519 0.136639976764603 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3511 0.13632934311466924 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3470 0.13473734565875886 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2915 0.11318713619460577 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2851 0.11070206699513588 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2758 0.10709095081465617 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2669 0.10363515145914333 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 3.8829206241717244E-5 0.0 7 0.0 0.0 0.0 3.8829206241717244E-5 0.0 8 0.0 0.0 0.0 3.8829206241717244E-5 0.0 9 0.0 0.0 0.0 7.765841248343449E-5 0.0 10-11 0.0 0.0 0.0 1.5531682496686898E-4 7.765841248343449E-5 12-13 0.0 0.0 0.0 2.7180444369202073E-4 1.747314280877276E-4 14-15 0.0 0.0 0.0 3.1063364993373795E-4 1.9414603120858622E-4 16-17 0.0 0.0 0.0 3.494628561754552E-4 2.3297523745030346E-4 18-19 0.0 0.0 0.0 3.6887745929631386E-4 2.3297523745030346E-4 20-21 0.0 0.0 0.0 4.659504749006069E-4 2.3297523745030346E-4 22-23 0.0 0.0 0.0 4.659504749006069E-4 2.7180444369202073E-4 24-25 0.0 0.0 0.0 6.212672998674759E-4 2.7180444369202073E-4 26-27 0.0 0.0 0.0 0.001281363805976669 3.494628561754552E-4 28-29 0.0 0.0 0.0 0.004038237449138594 3.494628561754552E-4 30-31 0.0 0.0 0.0 0.011396372031944012 3.6887745929631386E-4 32-33 0.0 0.0 0.0 0.028209418334607578 4.271212686588897E-4 34-35 0.0 0.0 0.0 0.05492391222890904 5.047796811423242E-4 36-37 0.0 0.0 0.0 0.0985291108383575 5.047796811423242E-4 38-39 0.0 0.0 0.0 0.16733446429868049 5.047796811423242E-4 40-41 0.0 0.0 0.0 0.23784830283363897 5.047796811423242E-4 42-43 0.0 0.0 0.0 0.307876776290576 5.241942842631828E-4 44-45 0.0 0.0 0.0 0.3949124420813852 5.436088873840415E-4 46-47 0.0 0.0 0.0 0.49466467291635685 5.436088873840415E-4 48-49 0.0 0.0 0.0 0.5986881164379174 6.212672998674759E-4 50-51 0.0 0.0 0.0 0.7136419815165213 6.212672998674759E-4 52-53 0.0 0.0 0.0 0.8184614237660369 6.600965061091932E-4 54-55 0.0 0.0 0.0 0.9333182158290365 6.600965061091932E-4 56-57 0.0 0.0 0.0 1.0665412224443684 6.600965061091932E-4 58-59 0.0 0.0 0.0 1.2080930937985488 6.600965061091932E-4 60-61 0.0 0.0 0.0 1.3578185130666103 6.600965061091932E-4 62-63 0.0 0.0 0.0 1.5054665698007401 6.989257123509104E-4 64-65 0.0 0.0 0.0 1.6556967687499442 7.377549185926277E-4 66-67 0.0 0.0 0.0 1.8283896635099817 7.765841248343449E-4 68-69 0.0 0.0 0.0 2.019157553775538 7.765841248343449E-4 70-71 0.0 0.0 0.0 2.2159633856116825 8.154133310760621E-4 72-73 0.0 0.0 0.0 2.4196031577463684 9.513155529220725E-4 74-75 0.0 0.0 0.0 2.619670642906817 0.0010095593622846484 76-77 0.0 1.9414603120858622E-5 0.0 2.8340466905673374 0.0010095593622846484 78-79 0.0 3.8829206241717244E-5 0.0 3.0758555724376313 0.0010483885685263657 80-81 0.0 3.8829206241717244E-5 0.0 3.333720331088876 0.0010678031716472242 82-83 0.0 3.8829206241717244E-5 0.0 3.597991908770003 0.001087217774768083 84-85 0.0 3.8829206241717244E-5 0.0 3.860865635026429 0.001087217774768083 86-87 0.0 3.8829206241717244E-5 0.0 4.1342814907774805 0.001087217774768083 88-89 0.0 3.8829206241717244E-5 0.0 4.426296536318316 0.001087217774768083 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5635 0.0 55.538635 1 GTATCAA 10175 0.0 41.275227 1 ATCAACG 12990 0.0 32.032154 3 TATCAAC 13035 0.0 31.927156 2 TCAACGC 13175 0.0 31.581137 4 CAACGCA 13385 0.0 31.155499 5 AACGCAG 13855 0.0 30.098421 6 TGGTATC 2505 0.0 28.100704 2 ACGCAGA 15845 0.0 26.258358 7 CGCAGAG 15930 0.0 26.058619 8 TAAGGTG 1765 0.0 24.246624 5 GTAAGGT 1745 0.0 23.980463 4 GTACATG 9955 0.0 23.315342 1 GTGGTAT 3030 0.0 23.231771 1 GCAGAGT 17570 0.0 23.220797 9 TACATGG 9450 0.0 23.202368 2 GAGTACT 11105 0.0 22.176138 12-13 ACATGGG 9670 0.0 21.834524 3 CAGAGTA 16690 0.0 21.215195 10-11 GTACTTT 11570 0.0 21.07962 14-15 >>END_MODULE