FastQCFastQC Report
Thu 26 May 2016
SRR938262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560640
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125020.4882373156710822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98600.38505998500374905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63260.24704761309672585No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39310.1535163084228943No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37410.14609628842789305No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35660.1392620594851287No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33610.13125624843789052No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC32050.12516402149462635No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28430.11102693076730817No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27690.10813702824293928No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA26980.10536428392901775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56050.055.9523471
GTATCAA100350.043.048671
TATCAAC130250.033.0571062
ATCAACG130400.032.946193
TGGTATC23550.032.893572
TCAACGC132600.032.399574
CAACGCA132950.032.2427865
AACGCAG139000.030.938966
GTGGTAT27750.028.3824231
ACGCAGA155700.027.4593357
CGCAGAG157050.027.0720548
GTACATG101350.026.4692171
TACATGG98950.026.1755052
ACATGGG100100.024.972733
GCAGAGT169450.024.556889
TAAGGTG15500.023.3021625
GAGTACT109000.023.01030212-13
GTAAGGT16150.022.3643044
CAGAGTA162350.021.82651710-11
GTACTTT115150.021.55699214-15