##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938262_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2560640 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.894889168332917 33.0 31.0 34.0 30.0 34.0 2 32.08448395713572 34.0 31.0 34.0 30.0 34.0 3 32.16812749937515 34.0 31.0 34.0 30.0 34.0 4 35.68863994938765 37.0 35.0 37.0 35.0 37.0 5 35.69791536490877 37.0 35.0 37.0 35.0 37.0 6 35.72471569607598 37.0 35.0 37.0 35.0 37.0 7 35.66915263371657 37.0 35.0 37.0 33.0 37.0 8 35.637312546863285 37.0 35.0 37.0 33.0 37.0 9 37.282439936890775 39.0 37.0 39.0 34.0 39.0 10-11 37.19942377686829 39.0 37.0 39.0 34.0 39.0 12-13 37.100666434953766 39.0 37.0 39.0 33.0 39.0 14-15 38.292631529617594 40.0 38.0 41.0 33.5 41.0 16-17 38.14645635466134 40.0 38.0 41.0 33.0 41.0 18-19 38.29760255248688 40.0 38.0 41.0 33.5 41.0 20-21 38.28367849443889 40.0 38.0 41.0 34.0 41.0 22-23 38.19936090196201 40.0 38.0 41.0 33.5 41.0 24-25 38.09434809266433 40.0 38.0 41.0 33.0 41.0 26-27 37.92074989065234 40.0 38.0 41.0 33.0 41.0 28-29 37.70741259997501 40.0 38.0 41.0 32.5 41.0 30-31 37.4860368892152 40.0 37.0 41.0 32.0 41.0 32-33 37.22604231754561 40.0 36.5 41.0 31.5 41.0 34-35 36.9958754452012 40.0 36.0 41.0 31.0 41.0 36-37 36.735664326105976 39.0 36.0 41.0 30.0 41.0 38-39 36.439613534116475 39.0 35.0 41.0 30.0 41.0 40-41 36.21911299518871 39.0 35.0 40.5 30.0 41.0 42-43 36.034652274431394 39.0 35.0 41.0 29.0 41.0 44-45 35.76188882466883 39.0 35.0 40.5 27.5 41.0 46-47 35.37477525149963 39.0 35.0 40.0 26.5 41.0 48-49 34.97157292708073 38.0 34.5 40.0 24.5 41.0 50-51 34.25363190452387 37.5 33.5 39.5 23.5 40.5 52-53 34.36311800956011 38.0 34.0 40.0 24.0 40.5 54-55 34.4649626655836 38.0 34.0 40.0 23.5 41.0 56-57 34.170291216258434 38.0 34.0 40.0 23.0 41.0 58-59 33.843342875218696 38.0 33.5 40.0 22.0 41.0 60-61 33.41936488534117 37.0 33.0 40.0 19.5 41.0 62-63 32.889438773119224 36.5 32.5 39.5 17.5 41.0 64-65 32.37267987690578 36.0 32.0 39.0 13.0 40.5 66-67 31.84156792833042 35.5 31.0 39.0 8.0 40.0 68-69 31.243897033554113 35.0 30.5 38.0 6.5 40.0 70-71 30.701641972319422 35.0 30.0 37.5 2.0 40.0 72-73 30.06805115127468 34.5 29.0 37.0 2.0 39.0 74-75 29.399867220694826 34.0 28.5 36.0 2.0 39.0 76-77 28.74853864658835 34.0 27.5 36.0 2.0 38.5 78-79 28.029091359972504 34.0 26.0 35.0 2.0 37.0 80-81 27.380229747250688 33.0 25.5 35.0 2.0 37.0 82-83 26.6519034303924 32.5 24.5 35.0 2.0 36.0 84-85 26.00833951668333 32.0 22.5 35.0 2.0 36.0 86-87 25.34744087415646 32.0 19.5 34.5 2.0 35.0 88-89 24.545055923519122 31.0 16.5 34.0 2.0 35.0 90-91 23.72607043551612 31.0 7.0 34.0 2.0 35.0 92-93 22.837856746750813 30.0 2.0 34.0 2.0 35.0 94-95 21.894633568170455 29.5 2.0 34.0 2.0 35.0 96-97 20.708922378780304 29.0 2.0 34.0 2.0 35.0 98-99 19.222717953324167 26.5 2.0 34.0 2.0 35.0 100-101 15.534677658710322 12.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40959485074565904 1101 2 0.347249371540137 1101 3 0.35050470639427544 1101 4 0.2350759913483529 1101 5 0.3322875192454475 1101 6 0.37565922319716805 1101 7 0.24802034148075336 1101 8 0.23030255180665193 1101 9 0.31888142738772984 1101 10-11 0.37323490646060264 1101 12-13 0.4264652569207783 1101 14-15 0.5148876341236175 1101 16-17 0.6239611352394618 1101 18-19 0.5729920985003716 1101 20-21 0.5261733076558457 1101 22-23 0.6400872386941288 1101 24-25 0.6861451487029129 1101 26-27 0.6613390352610011 1101 28-29 0.6646915357168126 1101 30-31 0.7456498451623617 1101 32-33 0.6284323498588122 1101 34-35 0.8042915272914044 1101 36-37 0.8543344769587975 1101 38-39 0.8581359499805714 1101 40-41 0.8703610665836621 1101 42-43 0.9422707140896023 1101 44-45 0.8797294146359036 1101 46-47 0.8126586250875647 1101 48-49 0.8909559762742489 1101 50-51 0.9146774436185083 1101 52-53 1.044426601643572 1101 54-55 1.0415866093785908 1101 56-57 1.1424512782638274 1101 58-59 1.174473084452245 1101 60-61 1.35177266775964 1101 62-63 1.2382521680449088 1101 64-65 1.0140575114761958 1101 66-67 1.3033718037845823 1101 68-69 1.3420969774202547 1101 70-71 1.6104126986260958 1101 72-73 1.834360247862687 1101 74-75 1.8914601012739638 1101 76-77 2.094897080351071 1101 78-79 2.1252708646177503 1101 80-81 2.3409518382000023 1101 82-83 2.4674722177955637 1101 84-85 2.636638481754485 1101 86-87 2.9704584948924726 1101 88-89 3.284693490393149 1101 90-91 3.6012826222687053 1101 92-93 3.882615551097853 1101 94-95 3.7930807994279725 1101 96-97 4.06465158512216 1101 98-99 4.163091783864303 1101 100-101 4.093444031494073 1102 1 0.3651936554613435 1102 2 0.25677777389511647 1102 3 0.237713384548762 1102 4 0.15503155570683447 1102 5 0.2722491691052227 1102 6 0.24709439682053613 1102 7 0.01763244818370424 1102 8 0.17274720101482188 1102 9 0.2847983143667676 1102 10-11 0.2180280711796243 1102 12-13 0.262085676648141 1102 14-15 0.3982584277361383 1102 16-17 0.42193110386467936 1102 18-19 0.40403403966045914 1102 20-21 0.478590141530276 1102 22-23 0.5145608571848683 1102 24-25 0.45533385378286795 1102 26-27 0.43038636725667345 1102 28-29 0.49487662681275424 1102 30-31 0.5755708484663273 1102 32-33 0.5071794542222889 1102 34-35 0.5085684577875256 1102 36-37 0.549964685157569 1102 38-39 0.5583912826881345 1102 40-41 0.7328682070008625 1102 42-43 0.7277869339599121 1102 44-45 0.6609262203218336 1102 46-47 0.7716912887287108 1102 48-49 0.8023667217768562 1102 50-51 0.8293605813259433 1102 52-53 0.7573437957681932 1102 54-55 0.8146017169226027 1102 56-57 0.7763208246356257 1102 58-59 0.70195041452466 1102 60-61 0.7963077727775882 1102 62-63 0.7902875098807272 1102 64-65 0.8428468124759974 1102 66-67 1.0026942176618228 1102 68-69 1.0891625888839656 1102 70-71 1.2991859534220147 1102 72-73 1.3397166372084648 1102 74-75 1.3616131573725276 1102 76-77 1.5602714391487815 1102 78-79 1.8368044683540212 1102 80-81 1.8850028301080108 1102 82-83 2.168106459074206 1102 84-85 2.2512568892476885 1102 86-87 2.316450819123393 1102 88-89 2.71545871347557 1102 90-91 3.0001125985295083 1102 92-93 3.1412041453757595 1102 94-95 3.621792596401871 1102 96-97 3.4519786722051116 1102 98-99 3.631381155191832 1102 100-101 3.450192192208714 1103 1 0.46158612628910944 1103 2 0.3249794367021437 1103 3 0.4325694531757591 1103 4 0.21094635969498654 1103 5 0.1872071208689121 1103 6 0.23416988960955365 1103 7 0.30128966753053277 1103 8 0.3625983976425218 1103 9 0.5203436619238104 1103 10-11 0.5358979037318932 1103 12-13 0.4488670919041766 1103 14-15 0.6433987314732974 1103 16-17 0.5824448502141948 1103 18-19 0.5022055284112739 1103 20-21 0.5328148609157708 1103 22-23 0.7011134361105533 1103 24-25 0.65816017579602 1103 26-27 0.681170245930538 1103 28-29 0.8175298768510686 1103 30-31 0.734453213234957 1103 32-33 0.8565499609805158 1103 34-35 0.9709820026485758 1103 36-37 0.9528059114109695 1103 38-39 0.9925493407499886 1103 40-41 0.9663547306149454 1103 42-43 1.1296439179322277 1103 44-45 1.2042740079560943 1103 46-47 1.3363781520469828 1103 48-49 1.4397258169488723 1103 50-51 1.3851864519936896 1103 52-53 1.2763618172443572 1103 54-55 1.5365047634336761 1103 56-57 1.5442435250589952 1103 58-59 1.6607524932443596 1103 60-61 1.5466958454484185 1103 62-63 1.7232345984217545 1103 64-65 1.8542991744465667 1103 66-67 1.9920591282996547 1103 68-69 2.01548924202433 1103 70-71 2.1000044914847713 1103 72-73 2.2079575291006996 1103 74-75 2.0817554647457683 1103 76-77 2.295791477618593 1103 78-79 2.45496872995437 1103 80-81 2.448757826895161 1103 82-83 2.649704292053915 1103 84-85 2.660909775116359 1103 86-87 2.7428182480154 1103 88-89 2.8193451347284544 1103 90-91 3.100299323350832 1103 92-93 3.3205931980706858 1103 94-95 3.6426043994353066 1103 96-97 3.9442091339412855 1103 98-99 4.162928932421245 1103 100-101 3.9633409660537424 1104 1 0.42135223330337723 1104 2 0.3233690658369994 1104 3 0.3022774196384148 1104 4 0.21678813942457253 1104 5 0.31266868160853534 1104 6 0.23400226260650925 1104 7 0.22786989325970097 1104 8 0.17767851524587286 1104 9 0.2709127619971312 1104 10-11 0.4129200377473339 1104 12-13 0.3737636899290777 1104 14-15 0.5529995787360988 1104 16-17 0.63046380187577 1104 18-19 0.5243919006344981 1104 20-21 0.5188186459590938 1104 22-23 0.41975620334659425 1104 24-25 0.4992170405909704 1104 26-27 0.5552397134610132 1104 28-29 0.5447635279932541 1104 30-31 0.6258397657958312 1104 32-33 0.4636819567953836 1104 34-35 0.5389161512548242 1104 36-37 0.5480101583029366 1104 38-39 0.6008755190121349 1104 40-41 0.5482016005752044 1104 42-43 0.5961379245204412 1104 44-45 0.6286868122705513 1104 46-47 0.6538361845154341 1104 48-49 0.8724602569616522 1104 50-51 0.7631366126042423 1104 52-53 0.7494924455982286 1104 54-55 1.0321725864280964 1104 56-57 1.1275683277560447 1104 58-59 1.1527403063773676 1104 60-61 1.3008027070230668 1104 62-63 1.4127857632588103 1104 64-65 1.403564238803817 1104 66-67 1.4265336048491442 1104 68-69 1.6120637223699532 1104 70-71 1.7770797073845124 1104 72-73 1.821312090922433 1104 74-75 1.8533276311563043 1104 76-77 1.671674878322623 1104 78-79 1.880443385220694 1104 80-81 1.8788815723051435 1104 82-83 2.156626690135205 1104 84-85 2.379990270626898 1104 86-87 2.5821349696406912 1104 88-89 2.759144647714429 1104 90-91 2.733280746610724 1104 92-93 2.9928263181807644 1104 94-95 3.229731626624492 1104 96-97 3.461783630921655 1104 98-99 3.6395267312611637 1104 100-101 3.383900268691285 1105 1 0.2547662269585338 1105 2 0.1761060852169436 1105 3 0.04381377760371663 1105 4 0.10970050393637365 1105 5 0.07923088014871382 1105 6 0.254885805363962 1105 7 0.24161694899532904 1105 8 0.23301669990727447 1105 9 0.3145776417774755 1105 10-11 0.22060501495754892 1105 12-13 0.19411965563803335 1105 14-15 0.3335753698414834 1105 16-17 0.38513071702826807 1105 18-19 0.2640250978210119 1105 20-21 0.393866212781937 1105 22-23 0.30636502875668015 1105 24-25 0.3199346767751976 1105 26-27 0.3117140052601357 1105 28-29 0.35462698437910944 1105 30-31 0.3927579430546899 1105 32-33 0.493001170669487 1105 34-35 0.5211025793750892 1105 36-37 0.5341245489499826 1105 38-39 0.6327999311837829 1105 40-41 0.7159049800475401 1105 42-43 0.6805715557115235 1105 44-45 0.6781660653299468 1105 46-47 0.776126827072467 1105 48-49 0.9485250545205091 1105 50-51 0.9627891227339518 1105 52-53 0.7969784455032638 1105 54-55 0.9912947159126233 1105 56-57 0.9283921363412944 1105 58-59 1.0437382892188296 1105 60-61 1.0618289404917647 1105 62-63 1.0721629159624655 1105 64-65 1.0760685336407079 1105 66-67 1.1846585060565111 1105 68-69 1.2963779242458315 1105 70-71 1.3104307989983717 1105 72-73 1.3770836621920104 1105 74-75 1.5875972421849127 1105 76-77 1.7138223357986995 1105 78-79 1.8555383725069383 1105 80-81 2.0479989467336104 1105 82-83 2.197709951752408 1105 84-85 2.301104963750152 1105 86-87 2.5921119684392444 1105 88-89 2.734418844022141 1105 90-91 2.740025152914974 1105 92-93 3.2155361342166877 1105 94-95 3.318562383706176 1105 96-97 3.1553653134829567 1105 98-99 3.4083937659730736 1105 100-101 3.3964183928704514 1106 1 0.17432853712142915 1106 2 0.16468433274506822 1106 3 0.07364982684343602 1106 4 0.17477826028166277 1106 5 0.16394629768527125 1106 6 -0.0503788028496146 1106 7 0.10533075831675376 1106 8 0.2116609107220171 1106 9 0.13827958798244566 1106 10-11 0.18720738415578353 1106 12-13 0.21986915706020227 1106 14-15 0.3912035716763711 1106 16-17 0.2623266779640474 1106 18-19 0.28988186547984895 1106 20-21 0.2505688735762064 1106 22-23 0.14173657627063818 1106 24-25 0.1897170461913973 1106 26-27 0.14006833257317197 1106 28-29 0.14391538421284622 1106 30-31 0.1734397453142904 1106 32-33 0.2417971110549928 1106 34-35 0.2689070465701562 1106 36-37 0.2856525090077042 1106 38-39 0.016292918400573342 1106 40-41 0.4185744926166848 1106 42-43 0.2772275424597126 1106 44-45 0.382632949020028 1106 46-47 0.5619305532119583 1106 48-49 0.6218457307492784 1106 50-51 0.6511473110087707 1106 52-53 0.7061167037424454 1106 54-55 0.2773631047550751 1106 56-57 0.6718186153776671 1106 58-59 0.7298590657080695 1106 60-61 0.810115327332106 1106 62-63 0.5557233921685025 1106 64-65 0.869479755650687 1106 66-67 0.9258741952615388 1106 68-69 0.6059591033026024 1106 70-71 0.8314601967973125 1106 72-73 1.1934219135810977 1106 74-75 1.217264919240364 1106 76-77 1.4660918184765244 1106 78-79 1.7154029741817816 1106 80-81 1.8420078394004271 1106 82-83 1.854228749810094 1106 84-85 2.0839034448976044 1106 86-87 2.105120218131379 1106 88-89 2.339678703875453 1106 90-91 2.550235061575208 1106 92-93 2.9210223585230004 1106 94-95 2.7390728090814136 1106 96-97 2.782803051582089 1106 98-99 3.2051999098841932 1106 100-101 3.292216094041354 1107 1 0.31172670242782274 1107 2 0.20307922064716166 1107 3 0.13761781540145535 1107 4 0.07861574613125555 1107 5 0.18482213168748274 1107 6 0.1772315109592526 1107 7 0.2106974522332976 1107 8 0.16266646109300353 1107 9 0.22096691114875 1107 10-11 0.27066569109233996 1107 12-13 0.21801947849507997 1107 14-15 0.2977737361697166 1107 16-17 0.3783602917585256 1107 18-19 0.39654364596052716 1107 20-21 0.32673328681104863 1107 22-23 0.4007770341873709 1107 24-25 0.23954256780906036 1107 26-27 0.4038497509221841 1107 28-29 0.5340344330682711 1107 30-31 0.5764161892490165 1107 32-33 0.4896822283160276 1107 34-35 0.6396921547808674 1107 36-37 0.6581291376580651 1107 38-39 0.700971016170783 1107 40-41 0.7659267534915415 1107 42-43 0.8803723075889565 1107 44-45 0.8175611918179726 1107 46-47 0.984602728212586 1107 48-49 0.9140334463435806 1107 50-51 0.841860862311492 1107 52-53 0.7853536278784716 1107 54-55 0.884281706732196 1107 56-57 0.9915809627647221 1107 58-59 1.0360299773724506 1107 60-61 0.9998741436090484 1107 62-63 1.0801108261641943 1107 64-65 1.2019172305212038 1107 66-67 1.349111941345054 1107 68-69 1.4598887986034086 1107 70-71 1.4831414058019412 1107 72-73 1.6451663264895338 1107 74-75 1.8642602115880962 1107 76-77 1.9574198274497014 1107 78-79 2.001628380667455 1107 80-81 2.26181944428998 1107 82-83 2.3376929913855022 1107 84-85 2.4717878054921947 1107 86-87 2.4447190217081847 1107 88-89 2.6851199368560223 1107 90-91 2.760571051153981 1107 92-93 3.0759962535303345 1107 94-95 3.2489615494394783 1107 96-97 3.2614399837298755 1107 98-99 3.4932198769766245 1107 100-101 3.624715214972973 1108 1 0.11140780066559941 1108 2 0.07610661090743776 1108 3 0.1179562887921648 1108 4 0.09033757072359805 1108 5 0.0022373741786267942 1108 6 0.10740070868948237 1108 7 0.06404132841770149 1108 8 0.09378056324176498 1108 9 0.12797328695742038 1108 10-11 0.13786264535506376 1108 12-13 0.12155684438545222 1108 14-15 0.1709013224802689 1108 16-17 0.1934424970513291 1108 18-19 0.0950747440313151 1108 20-21 0.1834270509428606 1108 22-23 0.08483467363522124 1108 24-25 -0.004885975151111666 1108 26-27 0.017777669883621172 1108 28-29 0.18651598307890538 1108 30-31 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-2.842653080639808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6256.0 3 1929.0 4 2084.0 5 2191.0 6 2736.0 7 3304.0 8 4171.0 9 5345.0 10 6875.0 11 8882.0 12 11172.0 13 13818.0 14 16372.0 15 20417.0 16 25006.0 17 30662.0 18 29972.0 19 24647.0 20 24517.0 21 25795.0 22 28315.0 23 31159.0 24 35107.0 25 38944.0 26 44327.0 27 50970.0 28 59149.0 29 69568.0 30 81433.0 31 99410.0 32 121278.0 33 153433.0 34 203017.0 35 276142.0 36 364826.0 37 407897.0 38 216503.0 39 13011.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.08678000786227 18.28421245079215 14.532528379108959 23.09647916223662 2 15.609116331839104 19.628023781599342 40.363670317460446 24.39918956910111 3 19.413682258123124 23.745520798615573 29.474952223805534 27.36584471945576 4 12.699852883426232 16.428642600505235 36.75937159927243 34.1121329167961 5 13.669052657251424 37.27945481535216 34.03081281321501 15.020679714181414 6 32.46405348564621 35.98028291330431 17.024982089765743 14.530681511283742 7 28.922986279169173 32.94714600623724 19.999835903270448 18.130031811323143 8 26.896041406534472 34.32006457269038 19.34957704262704 19.434316978148114 9 26.57732595165443 16.828626015338994 19.970619146783147 36.62342888622342 10-11 25.224560125107814 26.496658875104153 26.654450225981364 21.624330773806673 12-13 26.300765989002155 23.89570781654162 27.184356016200812 22.619170178255416 14-15 22.852204141254752 25.270084804264943 25.87078982005025 26.006921234430052 16-17 22.13924852513814 28.43453187774171 26.799052134738137 22.627167462382012 18-19 22.124650679398655 26.636432099693973 29.377176385780835 21.861740835126536 20-21 22.57831267866716 25.766989107652776 29.932385860110937 21.722312353569123 22-23 22.355889401170124 25.802701461417836 29.78712555917415 22.054283578237886 24-25 22.08417244019733 26.04658696559875 29.852183677309345 22.01705691689457 26-27 21.583863733960502 26.369484715904672 29.691618061533376 22.35503348860145 28-29 21.89022984688595 26.840689501588184 29.449527843002425 21.81955280852344 30-31 21.8508819653973 26.279277158566234 29.42782378859789 22.442017087438572 32-33 21.516224513795933 26.338433504380898 29.46976036931896 22.67558161250421 34-35 22.4296036455405 26.489733339949467 29.198023287480158 21.88263972702988 36-37 22.542752553158778 25.947094490756196 29.185350956651927 22.3248019994331 38-39 21.89836009028947 26.39179824440618 29.147162184517626 22.56267948078673 40-41 22.44277188676017 26.194073635419752 29.263290336239823 22.099864141580255 42-43 22.087892750134227 26.22226093688752 29.012197267972912 22.677649045005342 44-45 22.055380615768954 26.56738844626649 29.231839440428768 22.145391497535783 46-47 22.59052946553435 26.91641210340867 28.316191004476664 22.17686742658031 48-49 22.345903119907124 26.890370429399347 28.26123811889929 22.50248833179424 50-51 22.491058431009993 27.155575293342444 28.215635061340123 22.13773121430745 52-53 22.846308459545853 26.73818232097716 28.230840017623404 22.184669201853584 54-55 22.22144321022036 27.02928612077932 28.453790939304895 22.295479729695423 56-57 22.427215504444593 27.17812523130097 28.09903265173881 22.295626612515633 58-59 22.388783150040368 26.676576998474 28.14555064248556 22.78908920900007 60-61 21.97842029396193 26.976585497511063 28.51701380677006 22.527980401756952 62-63 22.165484609561812 26.91090552162192 28.65166981352428 22.271940055291996 64-65 21.879082905863058 26.986013294966792 28.41917306228339 22.715730736886755 66-67 21.852375211052408 27.00490512552224 28.370794525662763 22.77192513776259 68-69 22.475694520389574 26.631744057792595 28.458775594376974 22.433785827440857 70-71 22.18629158887266 26.789444492184145 28.09005616792569 22.934207751017507 72-73 22.217077323338472 26.924490547599085 28.089377244166485 22.76905488489596 74-75 22.245895524574124 27.29449306042421 27.942714080103425 22.516897334898246 76-77 22.423789489800065 26.833633545903623 28.04724871973529 22.695328244561022 78-79 22.513939903354167 27.076963577364317 27.864144334438944 22.544952184842572 80-81 22.760929649026323 27.12357120090993 27.58851679874009 22.526982351323653 82-83 22.71386499808613 27.220243256544258 27.61317209970862 22.452719645660988 84-85 22.6312976274846 27.347210746242773 27.623018322661082 22.39847330361155 86-87 22.842062406453294 27.317483238480754 27.3115860737087 22.528868281357244 88-89 22.65742190429761 27.386622165554137 27.158979755743896 22.79697617440436 90-91 22.697200789187946 27.276186286951194 27.248020535606333 22.77859238825453 92-93 22.877064581388932 26.88950953516172 27.46674181603287 22.76668406741648 94-95 22.622599296904514 26.688114764184867 27.630191618717387 23.059094320193232 96-97 23.02946163885322 26.30797277940041 27.5229465310767 23.139619050669673 98-99 22.71989897119679 26.572383793829996 27.14526054447119 23.562456690502025 100-101 23.660290178388095 25.569286474990037 27.297943047690488 23.472480298931377 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 970.0 1 673.5 2 557.5 3 737.0 4 608.0 5 558.0 6 1039.5 7 2315.5 8 3806.0 9 4382.0 10 3590.5 11 2681.5 12 2605.5 13 2793.5 14 2906.5 15 3066.0 16 3339.0 17 3625.0 18 3934.5 19 4314.0 20 4849.0 21 5632.5 22 6751.5 23 8269.0 24 10116.0 25 12620.0 26 16161.5 27 20068.5 28 23486.5 29 26762.0 30 30280.0 31 35617.5 32 44167.5 33 48459.0 34 49193.5 35 52238.5 36 56949.0 37 61999.0 38 65196.5 39 67862.0 40 71064.0 41 75131.0 42 77199.0 43 79116.0 44 81958.0 45 86623.0 46 89609.0 47 91807.0 48 92898.0 49 90548.5 50 89860.5 51 87872.5 52 86481.5 53 87680.0 54 89549.0 55 86191.5 56 79051.0 57 72624.0 58 67815.5 59 60152.5 60 48341.5 61 39918.5 62 33771.5 63 25698.5 64 18329.0 65 14050.0 66 10655.5 67 7988.0 68 6186.0 69 4817.0 70 3634.5 71 2560.0 72 1855.5 73 1464.5 74 1071.5 75 765.5 76 544.5 77 358.5 78 230.5 79 148.0 80 94.0 81 55.5 82 42.5 83 34.0 84 26.5 85 20.5 86 12.0 87 10.5 88 9.0 89 4.5 90 1.5 91 2.0 92 3.0 93 2.5 94 2.0 95 1.0 96 0.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45918207948012996 2 0.0395994751312172 3 0.05119813796550862 4 0.05643120469882529 5 0.08177643089227693 6 0.07994095226193453 7 0.04584791302174456 8 0.04077105723569108 9 0.04491064733816546 10-11 0.04871828917770558 12-13 0.04446154086478381 14-15 0.038310734816295926 16-17 0.03879889402649338 18-19 0.047156179705073735 20-21 0.05514246438390402 22-23 0.04582838665333667 24-25 0.0632459072731817 26-27 0.05510341164708823 28-29 0.043348537865533616 30-31 0.04520354286428393 32-33 0.043660959760059986 34-35 0.04838634091477131 36-37 0.0435242751812047 38-39 0.025442858035491126 40-41 0.02429080229942514 42-43 0.023412115721069732 44-45 0.015386778305423643 46-47 0.027336915771057235 48-49 0.027024493876530865 50-51 0.01589446388402899 52-53 0.01593351662084479 54-55 0.01628499125218695 56-57 0.015367251937015746 58-59 0.013629405148712822 60-61 0.016031148462884278 62-63 0.016167833041739566 64-65 0.01651930767308173 66-67 0.010036553361659584 68-69 0.012184453886528368 70-71 0.017964258935266184 72-73 0.015269620094976257 74-75 0.012692139465133716 76-77 0.009060234941264685 78-79 0.014215196200949761 80-81 0.017495626093476633 82-83 0.0148400399900025 84-85 0.013297456885778556 86-87 0.0034171144713821544 88-89 0.027493126718320417 90-91 0.030988346663334165 92-93 0.0158358847788053 94-95 0.031906085978505375 96-97 0.026048175456135966 98-99 0.022572481879530117 100-101 0.1483613471632092 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2560640.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.84591309409098 #Duplication Level Percentage of deduplicated Percentage of total 1 75.46225110455626 39.87871563755851 2 13.802996404799078 14.588638968921245 3 4.6872655509970045 7.431084838707426 4 2.0692010388674937 4.3739527309677735 5 1.0822153643867496 2.8595329547736092 6 0.6435603886833201 2.040572182269489 7 0.41054810092265076 1.5187052483611745 8 0.29795020052031673 1.259636032245091 9 0.211155488603022 1.0042834140050054 >10 1.1868068667561933 11.6160183369356 >50 0.0910431661983734 3.2707532140246696 >100 0.0484183408146931 4.688935684858475 >500 0.003182958511005595 1.2112813005312009 >1k 0.003182958511001962 3.1356792846923893 >5k 1.48044581907068E-4 0.6331599912928022 >10k+ 7.4022290953534E-5 0.489050179855592 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12502 0.4882373156710822 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9860 0.38505998500374905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6326 0.24704761309672585 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3931 0.1535163084228943 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3741 0.14609628842789305 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3566 0.1392620594851287 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3361 0.13125624843789052 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3205 0.12516402149462635 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2843 0.11102693076730817 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2769 0.10813702824293928 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2698 0.10536428392901775 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.905273681579605E-5 0.0 3 0.0 0.0 0.0 3.905273681579605E-5 0.0 4 0.0 0.0 0.0 3.905273681579605E-5 0.0 5 0.0 0.0 0.0 3.905273681579605E-5 0.0 6 0.0 0.0 0.0 3.905273681579605E-5 0.0 7 0.0 0.0 0.0 3.905273681579605E-5 0.0 8 0.0 0.0 0.0 3.905273681579605E-5 0.0 9 0.0 0.0 0.0 3.905273681579605E-5 3.905273681579605E-5 10-11 0.0 0.0 0.0 7.81054736315921E-5 3.905273681579605E-5 12-13 0.0 0.0 0.0 7.81054736315921E-5 1.9526368407898025E-4 14-15 0.0 0.0 0.0 7.81054736315921E-5 3.124218945263684E-4 16-17 0.0 0.0 0.0 7.81054736315921E-5 3.905273681579605E-4 18-19 0.0 0.0 0.0 1.1715821044738816E-4 3.905273681579605E-4 20-21 0.0 0.0 0.0 1.1715821044738816E-4 4.2958010497375657E-4 22-23 0.0 0.0 0.0 2.3431642089477632E-4 5.272119470132467E-4 24-25 0.0 0.0 0.0 4.6863284178955264E-4 5.857910522369408E-4 26-27 0.0 0.0 0.0 0.0010739502624343914 6.443701574606348E-4 28-29 0.0 0.0 0.0 0.0038466945763559112 6.638965258685328E-4 30-31 0.0 0.0 0.0 0.012164927518120469 6.638965258685328E-4 32-33 0.0 0.0 0.0 0.029191920769807547 7.02949262684329E-4 34-35 0.0 0.0 0.0 0.055825887278180455 7.42001999500125E-4 36-37 0.0 0.0 0.0 0.10013121719570107 7.42001999500125E-4 38-39 0.0 0.0 0.0 0.16878592851787053 7.42001999500125E-4 40-41 0.0 0.0 0.0 0.23980333041739565 7.42001999500125E-4 42-43 0.0 0.0 0.0 0.3137496875781055 7.42001999500125E-4 44-45 0.0 0.0 0.0 0.4016183454136466 7.61528367908023E-4 46-47 0.0 0.0 0.0 0.5049714133966509 8.005811047238191E-4 48-49 0.0 0.0 0.0 0.6132060734816296 8.591602099475131E-4 50-51 0.0 0.0 0.0 0.7298175456135966 8.982129467633091E-4 52-53 0.0 0.0 0.0 0.8383841539615096 8.982129467633091E-4 54-55 3.905273681579605E-5 0.0 0.0 0.9531601474631342 8.982129467633091E-4 56-57 3.905273681579605E-5 0.0 0.0 1.0851779242689328 8.982129467633091E-4 58-59 3.905273681579605E-5 0.0 0.0 1.227193201699575 8.982129467633091E-4 60-61 3.905273681579605E-5 0.0 0.0 1.3787568732816795 9.372656835791053E-4 62-63 3.905273681579605E-5 0.0 0.0 1.5278016433391652 9.372656835791053E-4 64-65 3.905273681579605E-5 0.0 0.0 1.673761247188203 9.372656835791053E-4 66-67 3.905273681579605E-5 0.0 0.0 1.8480536115971007 9.567920519870033E-4 68-69 3.905273681579605E-5 0.0 0.0 2.032987846788303 9.763184203949013E-4 70-71 3.905273681579605E-5 0.0 0.0 2.2304384841289675 0.0010153711572106973 72-73 3.905273681579605E-5 0.0 0.0 2.4340789802549363 0.0010739502624343914 74-75 3.905273681579605E-5 0.0 0.0 2.635591102224444 0.0011520557360659837 76-77 3.905273681579605E-5 0.0 0.0 2.8537592164458885 0.0011715821044738816 78-79 3.905273681579605E-5 0.0 0.0 3.09740924143964 0.0011715821044738816 80-81 3.905273681579605E-5 0.0 0.0 3.3503538177955514 0.0011715821044738816 82-83 3.905273681579605E-5 0.0 0.0 3.607477036990752 0.0012106348412896776 84-85 3.905273681579605E-5 0.0 0.0 3.8732504373906522 0.0012106348412896776 86-87 3.905273681579605E-5 0.0 0.0 4.139746313421645 0.0012887403149212696 88-89 3.905273681579605E-5 0.0 0.0 4.424245501124719 0.001366845788552862 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5605 0.0 55.952347 1 GTATCAA 10035 0.0 43.04867 1 TATCAAC 13025 0.0 33.057106 2 ATCAACG 13040 0.0 32.94619 3 TGGTATC 2355 0.0 32.89357 2 TCAACGC 13260 0.0 32.39957 4 CAACGCA 13295 0.0 32.242786 5 AACGCAG 13900 0.0 30.93896 6 GTGGTAT 2775 0.0 28.382423 1 ACGCAGA 15570 0.0 27.459335 7 CGCAGAG 15705 0.0 27.072054 8 GTACATG 10135 0.0 26.469217 1 TACATGG 9895 0.0 26.175505 2 ACATGGG 10010 0.0 24.97273 3 GCAGAGT 16945 0.0 24.55688 9 TAAGGTG 1550 0.0 23.302162 5 GAGTACT 10900 0.0 23.010302 12-13 GTAAGGT 1615 0.0 22.364304 4 CAGAGTA 16235 0.0 21.826517 10-11 GTACTTT 11515 0.0 21.556992 14-15 >>END_MODULE