FastQCFastQC Report
Thu 26 May 2016
SRR938262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560640
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120380.47011684578855284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99940.39029305173706574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63570.2482582479380155No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40380.15769495126218444No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37650.14703355411147212No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35430.13836384653836542No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34440.1344976255936016No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33000.12887403149212695No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28670.11196419645088727No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28390.110870719820045No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26150.10212290677330668No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA25950.10134185203699077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57950.053.605091
GTATCAA104350.040.9667741
ATCAACG130600.032.4034163
TATCAAC131800.032.0742382
TCAACGC133400.031.9006824
CAACGCA135250.031.39415
AACGCAG139900.030.3506246
TGGTATC26100.028.208042
ACGCAGA157800.026.7272197
CGCAGAG157650.026.5417618
GTGGTAT30650.025.260321
TAAGGTG17450.024.2238065
GTGTAAG11700.023.9523351
GCAGAGT171950.023.6991589
TACATGG94450.023.384752
GTACATG97750.023.1298031
GTAAGGT17200.022.9190944
GAGTACT109050.022.8655412-13
GTACTTT112500.021.9743514-15
ACATGGG96100.021.9440023
Produced by FastQC (version 0.11.5)