##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938262_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2560640 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49925253061735 33.0 31.0 34.0 31.0 34.0 2 32.73012957698076 34.0 31.0 34.0 31.0 34.0 3 32.69011809547613 34.0 31.0 34.0 31.0 34.0 4 36.21439054298925 37.0 35.0 37.0 35.0 37.0 5 36.17710377093227 37.0 35.0 37.0 35.0 37.0 6 36.23535795738565 37.0 37.0 37.0 35.0 37.0 7 36.20230840727318 37.0 37.0 37.0 35.0 37.0 8 36.19363518495376 37.0 37.0 37.0 35.0 37.0 9 37.90284694451387 39.0 38.0 39.0 35.0 39.0 10-11 37.87020276180955 39.0 38.0 39.0 35.0 39.0 12-13 37.81095253530367 39.0 38.0 39.0 35.0 39.0 14-15 39.08679861284679 40.0 38.5 41.0 36.0 41.0 16-17 39.02609992033241 40.0 38.0 41.0 36.0 41.0 18-19 39.02853681110972 40.0 38.5 41.0 36.0 41.0 20-21 39.03384778805299 40.0 38.5 41.0 35.5 41.0 22-23 39.05794313140465 40.0 39.0 41.0 35.5 41.0 24-25 39.00407847256936 40.0 38.5 41.0 35.0 41.0 26-27 38.83557450481129 40.0 38.0 41.0 35.0 41.0 28-29 38.75792672925519 40.0 38.0 41.0 35.0 41.0 30-31 38.53387200074981 40.0 38.0 41.0 34.0 41.0 32-33 38.324742642464386 40.0 38.0 41.0 34.0 41.0 34-35 38.1182471960135 40.0 38.0 41.0 34.0 41.0 36-37 38.02691729411397 40.0 38.0 41.0 33.0 41.0 38-39 37.96710529398901 40.0 38.0 41.0 33.0 41.0 40-41 37.83625714665084 40.0 37.5 41.0 33.0 41.0 42-43 37.63402762590602 40.0 37.0 41.0 33.0 41.0 44-45 37.37380498625343 40.0 37.0 41.0 32.5 41.0 46-47 37.00761918895276 40.0 36.5 41.0 31.5 41.0 48-49 36.617747320982254 40.0 36.0 41.0 30.5 41.0 50-51 36.30728353067983 39.0 35.5 41.0 30.0 41.0 52-53 35.840887043864036 39.0 35.0 40.0 29.0 41.0 54-55 35.5223018464134 38.5 35.0 40.0 28.0 41.0 56-57 35.242964649462635 38.0 35.0 40.0 27.5 41.0 58-59 34.85886692389403 38.0 34.0 40.0 26.5 41.0 60-61 34.4363495063734 37.5 34.0 40.0 26.0 41.0 62-63 34.00520065296176 37.0 33.5 40.0 25.5 41.0 64-65 33.56802459541365 36.0 33.0 39.0 24.0 41.0 66-67 33.051427182266934 36.0 32.5 39.0 22.5 40.0 68-69 32.519047386590856 35.0 32.0 38.5 21.5 40.0 70-71 31.91922820076231 35.0 31.0 37.5 19.5 40.0 72-73 31.452188515371155 35.0 31.0 37.0 18.0 39.0 74-75 30.904637121969508 34.5 30.0 36.5 15.0 39.0 76-77 29.692418692201947 33.5 28.5 35.0 13.5 37.5 78-79 30.38907050581105 34.0 30.0 36.0 12.5 37.5 80-81 30.209072731817045 34.0 30.0 35.0 8.5 37.0 82-83 29.751791349037738 34.0 30.0 35.0 6.5 37.0 84-85 29.249579987815544 34.0 29.0 35.0 2.0 36.0 86-87 28.71849381404649 34.0 29.0 35.0 2.0 36.0 88-89 28.094554486378406 33.0 27.5 35.0 2.0 35.0 90-91 27.561384263309172 33.0 26.5 35.0 2.0 35.0 92-93 26.936096444638842 33.0 25.5 35.0 2.0 35.0 94-95 26.197333869657584 32.0 24.5 34.5 2.0 35.0 96-97 25.37763898869033 32.0 21.5 34.0 2.0 35.0 98-99 24.36259528867783 31.5 10.0 34.0 2.0 35.0 100-101 20.85332514527618 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05471610271677463 1101 2 0.05686978092381878 1101 3 0.16848780251305584 1101 4 0.08929967071119194 1101 5 0.07140919652322708 1101 6 0.08496101112745436 1101 7 0.07656585021385354 1101 8 0.11626483926826836 1101 9 0.1547822183221399 1101 10-11 0.2159749731234868 1101 12-13 0.22081732588205938 1101 14-15 0.21194243948374947 1101 16-17 0.28777503040480923 1101 18-19 0.32336119405413655 1101 20-21 0.2425985854070305 1101 22-23 0.23599835722678364 1101 24-25 0.21344797921977232 1101 26-27 0.32896867711452416 1101 28-29 0.2781934055644655 1101 30-31 0.26655523458774866 1101 32-33 0.2514710718455646 1101 34-35 0.28348768969007665 1101 36-37 0.34426708814318374 1101 38-39 0.41026533898931206 1101 40-41 0.3494807477734341 1101 42-43 0.5058607470566869 1101 44-45 0.48138060771606916 1101 46-47 0.41590747562197095 1101 48-49 0.5076345301597556 1101 50-51 0.5718214578735683 1101 52-53 0.5164150782372516 1101 54-55 0.5117734432097052 1101 56-57 0.5677534077130773 1101 58-59 0.522103940480612 1101 60-61 0.6332777674968568 1101 62-63 0.7211216627064658 1101 64-65 0.6846220129499017 1101 66-67 0.7179775659486225 1101 68-69 0.7064692710266627 1101 70-71 0.7204738272795979 1101 72-73 0.6333933274179664 1101 74-75 0.7542476605313126 1101 76-77 0.7074147417420562 1101 78-79 0.8317999859863221 1101 80-81 1.0000175608517345 1101 82-83 1.076254499934791 1101 84-85 1.179551324172806 1101 86-87 1.3310330595236657 1101 88-89 1.5799915548971555 1101 90-91 1.7603739504965255 1101 92-93 1.9401103260028556 1101 94-95 1.9504704594096332 1101 96-97 2.215918441382602 1101 98-99 2.4964380601505844 1101 100-101 2.7249067865002132 1102 1 0.05511510492420513 1102 2 0.08459899908043411 1102 3 0.25804083392515054 1102 4 0.10231030024481669 1102 5 0.02324331494363463 1102 6 0.023966778001081934 1102 7 0.10743398141956106 1102 8 0.10984348719618708 1102 9 0.08934298578422784 1102 10-11 0.1639633130733742 1102 12-13 0.12430113184641556 1102 14-15 0.13127868926487452 1102 16-17 0.06793270803973428 1102 18-19 0.12146607709481572 1102 20-21 0.18657035097744767 1102 22-23 0.1785707445517133 1102 24-25 0.09224675293384621 1102 26-27 0.11132947844873797 1102 28-29 0.05454190385219704 1102 30-31 0.09353036727750919 1102 32-33 0.06770543827885689 1102 34-35 0.047906309027617056 1102 36-37 0.07686456380834272 1102 38-39 0.1534291968526773 1102 40-41 0.1909003344760407 1102 42-43 0.13185071746163146 1102 44-45 0.2568550328227772 1102 46-47 0.2439152776939153 1102 48-49 0.21145720094849452 1102 50-51 0.35426693953238697 1102 52-53 0.28665152292664686 1102 54-55 0.3453005366077946 1102 56-57 0.4015158837083632 1102 58-59 0.3698322113657895 1102 60-61 0.5323689610301514 1102 62-63 0.4256332581698956 1102 64-65 0.45380282410129524 1102 66-67 0.40068254256824076 1102 68-69 0.5201036644428427 1102 70-71 0.6900664144501008 1102 72-73 0.5186687107040733 1102 74-75 0.7593758435785958 1102 76-77 0.7479034189779767 1102 78-79 0.8469218825959501 1102 80-81 0.8598077545830982 1102 82-83 0.8035967720657773 1102 84-85 0.7939652071532137 1102 86-87 0.9288266347269705 1102 88-89 0.9465590451158619 1102 90-91 1.0475615303328496 1102 92-93 1.1650908782333929 1102 94-95 1.265322670714312 1102 96-97 1.5667928016155734 1102 98-99 1.9936787733024417 1102 100-101 2.2730030579824643 1103 1 0.2709116311113746 1103 2 0.30535751242464926 1103 3 0.37870401637414375 1103 4 0.24705367313062965 1103 5 0.10539872117557536 1103 6 0.07826672161805703 1103 7 0.1848270610714735 1103 8 0.22446271900914638 1103 9 0.3054783725281709 1103 10-11 0.26374544401415534 1103 12-13 0.27947360624557405 1103 14-15 0.4010289166951466 1103 16-17 0.36258848336252925 1103 18-19 0.3742469130539732 1103 20-21 0.3385366561459904 1103 22-23 0.43095296238703185 1103 24-25 0.5211391147919358 1103 26-27 0.504255518265559 1103 28-29 0.49800737874206646 1103 30-31 0.45655477508002207 1103 32-33 0.5766602854611875 1103 34-35 0.4658967815623356 1103 36-37 0.44627494643956567 1103 38-39 0.4432860880250047 1103 40-41 0.5352993631695142 1103 42-43 0.49168969542444785 1103 44-45 0.6196105315734926 1103 46-47 0.6306279365963121 1103 48-49 0.6390029084569946 1103 50-51 0.5938234258999202 1103 52-53 0.601306749885893 1103 54-55 0.7494859566050707 1103 56-57 0.6487617628968394 1103 58-59 0.762675996649115 1103 60-61 0.8711226572114299 1103 62-63 0.9451542977547192 1103 64-65 0.905624515957534 1103 66-67 0.8497139075576214 1103 68-69 0.9841331242203708 1103 70-71 1.183708209314318 1103 72-73 1.0164218261824125 1103 74-75 1.0510462011538166 1103 76-77 0.7950141757618674 1103 78-79 1.149890638527772 1103 80-81 1.3176839425283795 1103 82-83 1.5493149666279784 1103 84-85 1.595725805685003 1103 86-87 1.4969224914887853 1103 88-89 1.5687565909780368 1103 90-91 1.6511765819282367 1103 92-93 1.9200859078098738 1103 94-95 1.8248289044831019 1103 96-97 2.0930450038576787 1103 98-99 2.333057111817112 1103 100-101 2.561279537263463 1104 1 0.16406647794166673 1104 2 0.21426887100951575 1104 3 0.24071052439656881 1104 4 0.14268389734560571 1104 5 0.06112535864187407 1104 6 0.09609105960869613 1104 7 0.13259525467938005 1104 8 0.11287794431817844 1104 9 0.130073988378264 1104 10-11 0.1372647921370529 1104 12-13 0.10105342890218338 1104 14-15 0.2699127170594906 1104 16-17 0.17535711843086688 1104 18-19 0.19344967390909318 1104 20-21 0.1967709168021372 1104 22-23 0.17525620347432636 1104 24-25 0.2250710130163185 1104 26-27 0.2150095795948559 1104 28-29 0.1669470334474994 1104 30-31 0.20833650510159174 1104 32-33 0.23963382641314723 1104 34-35 0.36153862876926013 1104 36-37 0.3577211835972278 1104 38-39 0.39857979569972457 1104 40-41 0.4735024742072653 1104 42-43 0.4434928799962776 1104 44-45 0.3260254847552275 1104 46-47 0.27506024621064284 1104 48-49 0.24304350831427968 1104 50-51 0.27412876722987534 1104 52-53 0.29946394050850245 1104 54-55 0.3067154362661171 1104 56-57 0.48506557041839216 1104 58-59 0.491511701286079 1104 60-61 0.4958070769852725 1104 62-63 0.4069576273473672 1104 64-65 0.41272157881680727 1104 66-67 0.36242711481667556 1104 68-69 0.663551279376442 1104 70-71 0.7266336246816678 1104 72-73 0.6561285898253466 1104 74-75 0.6932841112161725 1104 76-77 0.6441054070781718 1104 78-79 0.799194474626951 1104 80-81 0.8542426241356047 1104 82-83 1.012229198331152 1104 84-85 1.2303602529914777 1104 86-87 1.2447763966647933 1104 88-89 1.3248193141874651 1104 90-91 1.296132459344701 1104 92-93 1.5270243648139719 1104 94-95 1.7533797071946502 1104 96-97 1.8572490464409093 1104 98-99 1.8650427325765833 1104 100-101 2.5030388573035047 1105 1 0.1125508769994994 1105 2 0.09746817160647225 1105 3 0.13034085944263296 1105 4 0.058723657680374686 1105 5 0.06820232615203281 1105 6 0.04373210296297003 1105 7 0.03203947904257376 1105 8 0.05690651108183431 1105 9 0.05071724143505918 1105 10-11 0.0855592491467192 1105 12-13 0.0068049326642665164 1105 14-15 0.027909309811377625 1105 16-17 0.06003676360350596 1105 18-19 0.1122100938891819 1105 20-21 0.09267031516719726 1105 22-23 0.11105985673642493 1105 24-25 0.09405441975705031 1105 26-27 0.1007365079782474 1105 28-29 0.11006654537449378 1105 30-31 0.03145427372486154 1105 32-33 0.0013219298613122987 1105 34-35 0.13967542086923146 1105 36-37 0.10091693253806966 1105 38-39 0.03448772194236227 1105 40-41 0.12186965560386653 1105 42-43 0.14516108506344239 1105 44-45 0.17517604700320533 1105 46-47 0.05540007335036279 1105 48-49 0.01959148722030335 1105 50-51 0.006640601204914276 1105 52-53 0.1467760653810899 1105 54-55 0.15215090768342066 1105 56-57 0.12676071213011397 1105 58-59 0.162948710453378 1105 60-61 0.1415033609512406 1105 62-63 0.181968669355129 1105 64-65 0.22217701756667196 1105 66-67 0.2938897466458954 1105 68-69 0.36033904710853193 1105 70-71 0.5307435282730637 1105 72-73 0.47989554394198564 1105 74-75 0.32072286628915236 1105 76-77 0.34375157496482345 1105 78-79 0.5806250049490487 1105 80-81 0.7791280246824002 1105 82-83 0.6745967999420159 1105 84-85 0.7975119223674199 1105 86-87 1.0138309341006142 1105 88-89 0.9543783298973629 1105 90-91 0.9000806845249016 1105 92-93 1.3235694360454922 1105 94-95 1.458012341710372 1105 96-97 1.5486603809073891 1105 98-99 1.596319617995345 1105 100-101 2.12612423170075 1106 1 0.008859766396959401 1106 2 0.054107546877943946 1106 3 0.10985316392306288 1106 4 0.04162893801266421 1106 5 0.06911587952905052 1106 6 0.0440839486739506 1106 7 0.06365375027825593 1106 8 -0.007593035099475287 1106 9 -0.0329846736331163 1106 10-11 0.008620478084630179 1106 12-13 -0.017416920068065167 1106 14-15 0.021062563527941336 1106 16-17 0.02948238865118924 1106 18-19 0.001814142917162087 1106 20-21 0.028322249462348736 1106 22-23 0.030568922990831027 1106 24-25 0.10133178782658803 1106 26-27 0.060501160561749145 1106 28-29 -0.07872312094811207 1106 30-31 0.03927739185627388 1106 32-33 0.1389278533239704 1106 34-35 -0.012208276998059375 1106 36-37 0.13805362459679316 1106 38-39 0.19349061299318038 1106 40-41 0.19312066949554207 1106 42-43 0.162658481249494 1106 44-45 0.26574191143051706 1106 46-47 -0.06350081358105797 1106 48-49 0.08191099114898748 1106 50-51 0.12067698765604717 1106 52-53 0.05198525011098809 1106 54-55 0.11607309996242776 1106 56-57 0.21559482621842818 1106 58-59 0.1781307750404224 1106 60-61 0.1755559541008367 1106 62-63 0.2748301713626802 1106 64-65 0.1465959661707359 1106 66-67 0.26443286564914104 1106 68-69 -0.00880992218591814 1106 70-71 0.22589700928818957 1106 72-73 0.26900163787856357 1106 74-75 0.40818875204516303 1106 76-77 0.48797406600132476 1106 78-79 0.8430633735681496 1106 80-81 0.861538852812 1106 82-83 0.8297435554108041 1106 84-85 0.8995228140160663 1106 86-87 1.108238689029175 1106 88-89 1.1833121128459254 1106 90-91 1.0945842629203284 1106 92-93 1.097210042776954 1106 94-95 1.4267345034753305 1106 96-97 1.7053046602230637 1106 98-99 1.9587164968436568 1106 100-101 2.1668042124582136 1107 1 0.07144973181284797 1107 2 0.05265860480150053 1107 3 0.14858350728642478 1107 4 0.04078740622303201 1107 5 0.06317664132896539 1107 6 0.01014671638180431 1107 7 -0.002049229949015796 1107 8 0.05211032197735932 1107 9 0.011085954503890605 1107 10-11 0.07183172600565513 1107 12-13 0.07693411975484565 1107 14-15 0.19598114770114705 1107 16-17 0.14994932603629252 1107 18-19 0.0906569476868313 1107 20-21 0.10787719605801982 1107 22-23 0.172219108670852 1107 24-25 0.06947108566978955 1107 26-27 0.130920472470045 1107 28-29 0.020615537734485656 1107 30-31 0.00828003128690824 1107 32-33 0.031230107448173783 1107 34-35 0.060080817064481096 1107 36-37 -0.03308474299177533 1107 38-39 0.07513470083176799 1107 40-41 0.03909950657339323 1107 42-43 0.009378480537336031 1107 44-45 0.07134717782903266 1107 46-47 0.0514319988888019 1107 48-49 0.1698953504082894 1107 50-51 0.08140629542494082 1107 52-53 0.1861617225319776 1107 54-55 0.19357021053512824 1107 56-57 0.24162437478528176 1107 58-59 0.3255023976745406 1107 60-61 0.3806677552785587 1107 62-63 0.4617480760197381 1107 64-65 0.3813276240566097 1107 66-67 0.3889725973144422 1107 68-69 0.5370132048049712 1107 70-71 0.6698180845260566 1107 72-73 0.47156599532779353 1107 74-75 0.55599849939275 1107 76-77 0.596937373612942 1107 78-79 0.7961682282510978 1107 80-81 0.7926489715196112 1107 82-83 1.0429615539835204 1107 84-85 1.067354104102698 1107 86-87 1.0799311293378402 1107 88-89 1.2048341548364618 1107 90-91 1.2889666079632 1107 92-93 1.3977416380523913 1107 94-95 1.4381437724910242 1107 96-97 1.5860874835978258 1107 98-99 1.6305434309053695 1107 100-101 2.080748314094837 1108 1 0.07921158584056798 1108 2 0.047844741230669285 1108 3 0.1032047161183769 1108 4 0.05071739144968035 1108 5 -0.11668389690195369 1108 6 -0.03629025640606187 1108 7 0.021255814699458142 1108 8 0.00870594964438709 1108 9 0.008443840322698293 1108 10-11 0.010382879985833426 1108 12-13 -0.00425515726384873 1108 14-15 0.055736736135855836 1108 16-17 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-2.7727742208197093 2316 98-99 -3.240308460703652 2316 100-101 -3.6931971961103365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 11.0 5 38.0 6 96.0 7 200.0 8 528.0 9 1051.0 10 1924.0 11 3171.0 12 4850.0 13 7075.0 14 9870.0 15 13691.0 16 17825.0 17 25188.0 18 25067.0 19 18961.0 20 16823.0 21 16774.0 22 17413.0 23 18774.0 24 21428.0 25 25034.0 26 29149.0 27 35000.0 28 42439.0 29 51459.0 30 62955.0 31 76986.0 32 97030.0 33 126952.0 34 177017.0 35 263833.0 36 405714.0 37 561759.0 38 360265.0 39 24276.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33084697575606 18.7522260059985 14.848944013996501 23.067983004248937 2 15.822723258765004 19.983432153261564 39.750451202379644 24.443393385593783 3 19.691432133269075 23.713382756473997 29.570931007305678 27.02425410295125 4 12.991947325668582 16.771549300174957 36.448895588602845 33.78760778555361 5 13.661961050179878 37.37130110269825 34.10862491545043 14.85811293167145 6 32.61326482951892 36.14535607559996 16.78149490985833 14.459884185022783 7 28.951394963759057 32.972108535366154 19.819459197700574 18.257037303174208 8 26.878290926600744 34.47264530455094 19.2202805627814 19.428783206066925 9 26.373953386653337 16.74112721819545 19.893229817545613 36.9916895776056 10-11 25.18247391277181 26.585658272931767 26.567713540364906 21.66415427393152 12-13 26.192533897775554 23.855930548612847 27.23877624343914 22.712759310172455 14-15 22.749449356410896 25.301213759060236 25.84867455011247 26.1006623344164 16-17 22.065464506034527 28.532227984454668 26.803929252829224 22.598378256681585 18-19 22.096585228692827 26.63576293426643 29.420750281179703 21.846901555861034 20-21 22.49023681579605 25.755982879280182 29.966609910022495 21.787170394901274 22-23 22.286303424143963 25.793805454886275 29.846073637840544 22.07381748312922 24-25 22.076386319235414 26.041019642837966 29.886618481392563 21.99597555653406 26-27 21.568548179978585 26.415500058884568 29.71940282649833 22.29654893463852 28-29 21.796403116650627 26.85520985334293 29.504905719619774 21.843481310386668 30-31 21.803159993720314 26.272280580074188 29.48690912186728 22.437650304338217 32-33 21.426320235480933 26.329706113576513 29.577879257830457 22.666094393112097 34-35 22.406183990150865 26.4510449085705 29.24070315722374 21.902067944054895 36-37 22.460055119550997 25.948115446811858 29.26930956445088 22.322519869186266 38-39 21.877499300317975 26.33948825657208 29.247353073122582 22.535659369987364 40-41 22.432151872775304 26.191608521359548 29.239278721503105 22.136960884362043 42-43 22.098110837408118 26.248815745762315 29.02540490216317 22.627668514666393 44-45 22.043525731494007 26.547110824957183 29.301765529400072 22.107597914148744 46-47 22.597654873209713 26.861912300170292 28.3876369730138 22.152795853606193 48-49 22.297032370939966 26.861924604491865 28.31381339748421 22.527229627083962 50-51 22.45807688702824 27.066944201449637 28.321064265183704 22.153914646338414 52-53 22.860085759810048 26.74438812171957 28.215328980254938 22.180197138215448 54-55 22.213372438140468 27.03103520994751 28.430490033741563 22.325102318170455 56-57 22.352517339415144 27.15149337665584 28.10096304048988 22.39502624343914 58-59 22.30028430392402 26.719726318420395 28.171316545863533 22.80867283179205 60-61 21.93127499375156 27.02418145463634 28.45856895151212 22.585974600099977 62-63 22.09191842664334 27.006685828542864 28.585724662584354 22.315671082229443 64-65 21.762000906023495 27.055306485878532 28.404422331917022 22.778270276180955 66-67 21.758594908009925 27.091638989553157 28.301497737177517 22.848268365259404 68-69 22.362892761846926 26.721073797196592 28.393629308383765 22.522404132572717 70-71 22.11083947763059 26.857582479380156 28.023306673331668 23.008271369657585 72-73 22.075657272333725 27.011045482974076 27.996111908763417 22.917185335928778 74-75 22.11513527868033 27.364408897775554 27.78668614096476 22.733769682579354 76-77 22.21138074856286 26.920965071232196 28.095378499125218 22.77227568107973 78-79 22.225361628342917 27.15914771307173 27.98362909272682 22.631861565858536 80-81 22.369177957811246 27.191887949668736 27.73435104964198 22.704583042878035 82-83 22.288458842279603 27.334813194246195 27.80950644008965 22.567221523384546 84-85 22.225416030400194 27.40598552632093 27.75443173430293 22.614166708975947 86-87 22.310070196150487 27.395009900959167 27.519003768827787 22.77591613406256 88-89 22.11313269851783 27.504755648882956 27.338000397557018 23.0441112550422 90-91 22.15068927566887 27.376515027842068 27.499791057576722 22.973004638912343 92-93 22.238617990955316 27.106111019987345 27.639536518499426 23.01573447055791 94-95 21.911887176533188 26.861045345153308 27.873488535569756 23.35357894274375 96-97 22.288471083885184 26.527992514345094 27.812539174914306 23.370997226855412 98-99 21.866470621545048 26.825357772583754 27.499917012772222 23.808254593098976 100-101 22.135958691921253 26.408278330813385 27.76963829655531 23.686124680710055 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 996.0 1 682.0 2 505.5 3 688.0 4 604.5 5 547.5 6 1068.5 7 2376.0 8 3873.5 9 4554.5 10 3820.0 11 2763.0 12 2592.0 13 2811.0 14 3010.0 15 3209.0 16 3439.5 17 3631.5 18 3870.5 19 4229.5 20 4757.0 21 5579.0 22 6628.0 23 8008.5 24 9855.0 25 12519.0 26 16010.5 27 19798.0 28 23281.0 29 26934.0 30 30658.5 31 35780.0 32 44630.5 33 48743.0 34 48976.5 35 52190.5 36 57349.0 37 62201.0 38 65167.5 39 68064.0 40 71218.5 41 75373.0 42 77493.5 43 79543.5 44 82743.5 45 87633.0 46 90838.0 47 93188.0 48 93564.0 49 90785.5 50 90433.5 51 88428.5 52 86736.5 53 88680.0 54 91703.0 55 87231.5 56 78963.5 57 72424.0 58 67427.0 59 59639.0 60 47335.0 61 38830.0 62 32811.5 63 24479.5 64 16833.5 65 12683.5 66 9566.5 67 7304.0 68 5571.0 69 4267.5 70 3277.0 71 2173.0 72 1529.5 73 1236.0 74 941.0 75 647.5 76 456.0 77 292.0 78 175.0 79 106.0 80 66.5 81 40.0 82 23.0 83 16.0 84 13.5 85 8.0 86 3.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010114658835291177 3 0.006482754311422145 4 0.0 5 4.686328417895526E-4 6 1.562109472631842E-4 7 0.0 8 3.905273681579605E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.9526368407898025E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001464477630592352 26-27 0.02134232066983254 28-29 2.538427893026743E-4 30-31 7.81054736315921E-5 32-33 0.0036319045238690324 34-35 0.016245938515371157 36-37 0.008025337415646087 38-39 0.021420426143464132 40-41 0.02352927393151712 42-43 0.018921050987253187 44-45 0.009626499625093727 46-47 0.002089321419645089 48-49 3.9052736815796055E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.1005373656585856E-4 68-69 2.9289552611847034E-4 70-71 0.0 72-73 1.9526368407898025E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.9526368407898027E-4 82-83 0.001523056735816046 84-85 0.005076855786053486 86-87 0.0011520557360659835 88-89 3.905273681579605E-5 90-91 0.004920644838790302 92-93 7.810547363159211E-4 94-95 9.763184203949014E-5 96-97 3.514746313421645E-4 98-99 1.9526368407898027E-4 100-101 0.002948481629592602 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2560640.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.95255367744179 #Duplication Level Percentage of deduplicated Percentage of total 1 73.68887506643304 37.546363622527316 2 14.760867107350448 15.042077472257173 3 4.991325280229032 7.6296230778732586 4 2.2519466631311884 4.589697329277112 5 1.1676570330853107 2.9747553827560855 6 0.7074880974217258 2.162899515601899 7 0.45411297684840574 1.619675107994389 8 0.31843694509406256 1.2980140430228646 9 0.2388381285824543 1.095247131013552 >10 1.2598269461349585 11.985336355222646 >50 0.10053217974340307 3.509579331972483 >100 0.0528900060412708 4.923635459825135 >500 0.0035251512299170743 1.2463750860537097 >1k 0.0034485175075221796 3.268220385418962 >5k 1.5326744477876352E-4 0.638454856893494 >10k+ 7.663372238938176E-5 0.47004584229000557 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12038 0.47011684578855284 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9994 0.39029305173706574 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6357 0.2482582479380155 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4038 0.15769495126218444 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3765 0.14703355411147212 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3543 0.13836384653836542 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3444 0.1344976255936016 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3300 0.12887403149212695 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2867 0.11196419645088727 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2839 0.110870719820045 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2615 0.10212290677330668 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2595 0.10134185203699077 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.905273681579605E-5 0.0 5 0.0 0.0 0.0 3.905273681579605E-5 0.0 6 0.0 0.0 0.0 3.905273681579605E-5 0.0 7 0.0 0.0 0.0 3.905273681579605E-5 0.0 8 0.0 0.0 0.0 3.905273681579605E-5 0.0 9 0.0 0.0 0.0 3.905273681579605E-5 0.0 10-11 0.0 0.0 0.0 3.905273681579605E-5 0.0 12-13 0.0 0.0 0.0 7.81054736315921E-5 1.1715821044738816E-4 14-15 0.0 0.0 0.0 7.81054736315921E-5 2.1479005248687826E-4 16-17 0.0 0.0 0.0 1.562109472631842E-4 3.905273681579605E-4 18-19 0.0 0.0 0.0 3.124218945263684E-4 3.905273681579605E-4 20-21 0.0 0.0 0.0 3.124218945263684E-4 4.2958010497375657E-4 22-23 0.0 0.0 0.0 4.1005373656585856E-4 4.4910647338165463E-4 24-25 0.0 0.0 0.0 6.638965258685328E-4 4.6863284178955264E-4 26-27 0.0 0.0 0.0 0.0012692139465133715 5.076855786053487E-4 28-29 0.0 0.0 0.0 0.004002905523619095 5.076855786053487E-4 30-31 0.0 0.0 0.0 0.012301612096975757 5.076855786053487E-4 32-33 0.0 0.0 0.0 0.029484816295926017 5.467383154211447E-4 34-35 0.0 0.0 0.0 0.05633357285678581 5.467383154211447E-4 36-37 0.0 0.0 0.0 0.10097085103724068 5.467383154211447E-4 38-39 0.0 0.0 0.0 0.1700160897275681 5.467383154211447E-4 40-41 0.0 0.0 0.0 0.24167786178455386 5.857910522369408E-4 42-43 0.0 0.0 0.0 0.3160733254186453 6.053174206448388E-4 44-45 0.0 0.0 0.0 0.4043910897275681 6.443701574606348E-4 46-47 0.0 0.0 0.0 0.5079198950262435 6.638965258685328E-4 48-49 0.0 0.0 0.0 0.6160569232691827 7.02949262684329E-4 50-51 0.0 0.0 0.0 0.7325707635591102 7.02949262684329E-4 52-53 0.0 0.0 0.0 0.8410397400649837 7.22475631092227E-4 54-55 0.0 0.0 0.0 0.9558157335666084 8.201074731317171E-4 56-57 0.0 0.0 0.0 1.088380248687828 8.201074731317171E-4 58-59 0.0 0.0 0.0 1.2313327918020494 8.591602099475131E-4 60-61 0.0 0.0 0.0 1.3840094663834042 8.982129467633091E-4 62-63 0.0 0.0 0.0 1.534147713071732 9.372656835791053E-4 64-65 0.0 0.0 0.0 1.6806540552361908 9.372656835791053E-4 66-67 0.0 0.0 0.0 1.8560789490127467 9.372656835791053E-4 68-69 0.0 0.0 0.0 2.041989502624344 9.372656835791053E-4 70-71 0.0 1.9526368407898025E-5 0.0 2.2396744563859032 9.763184203949013E-4 72-73 0.0 3.905273681579605E-5 0.0 2.4449356410897276 0.0010544238940264933 74-75 0.0 3.905273681579605E-5 0.0 2.648966664583854 0.0010934766308422893 76-77 0.0 3.905273681579605E-5 0.0 2.869516995751062 0.0010934766308422893 78-79 0.0 3.905273681579605E-5 0.0 3.1167793989002748 0.0010934766308422893 80-81 0.0 3.905273681579605E-5 0.0 3.374097881779555 0.0011130029992501875 82-83 0.0 3.905273681579605E-5 0.0 3.6358683766558357 0.0012301612096975757 84-85 0.0 3.905273681579605E-5 0.0 3.9068553174206446 0.0012496875781054736 86-87 0.0 3.905273681579605E-5 0.0 4.179189577605598 0.0013277930517370659 88-89 0.0 3.905273681579605E-5 0.0 4.472690421144714 0.001405898525368658 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5795 0.0 53.60509 1 GTATCAA 10435 0.0 40.966774 1 ATCAACG 13060 0.0 32.403416 3 TATCAAC 13180 0.0 32.074238 2 TCAACGC 13340 0.0 31.900682 4 CAACGCA 13525 0.0 31.3941 5 AACGCAG 13990 0.0 30.350624 6 TGGTATC 2610 0.0 28.20804 2 ACGCAGA 15780 0.0 26.727219 7 CGCAGAG 15765 0.0 26.541761 8 GTGGTAT 3065 0.0 25.26032 1 TAAGGTG 1745 0.0 24.223806 5 GTGTAAG 1170 0.0 23.952335 1 GCAGAGT 17195 0.0 23.699158 9 TACATGG 9445 0.0 23.38475 2 GTACATG 9775 0.0 23.129803 1 GTAAGGT 1720 0.0 22.919094 4 GAGTACT 10905 0.0 22.86554 12-13 GTACTTT 11250 0.0 21.97435 14-15 ACATGGG 9610 0.0 21.944002 3 >>END_MODULE