FastQCFastQC Report
Thu 26 May 2016
SRR938261_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938261_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583702
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129840.5025347350429732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100300.3882026642391421No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63980.24762917704905596No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40230.15570681138923917No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37100.14359241119912436No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35620.1378641964127442No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34150.13217468578032607No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC31630.12242123898189496No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28190.1091070100189573No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28100.10875867263329905No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA27660.1070556898589698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53100.056.383391
GTATCAA101350.044.476071
ATCAACG129300.034.39843
TATCAAC130150.034.1372342
TCAACGC131400.033.812494
CAACGCA133200.033.3555685
AACGCAG136800.032.4083146
CGCAGAG156100.028.2025138
ACGCAGA156650.028.1338447
TGGTATC24200.027.2935682
GTGGTAT26200.026.7913251
TAAGGTG17600.026.4590535
GCAGAGT171000.025.2719699
TACATGG96050.025.0330642
GTACATG100900.024.7034441
GTAAGGT18400.023.759154
GAGTACT108250.023.6469112-13
ACATGGG98900.023.2066123
GTGTAAG10850.022.4449461
AGGTAAG18200.022.1926122
Produced by FastQC (version 0.11.5)