##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938261_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2583702 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93041264046705 33.0 31.0 34.0 30.0 34.0 2 32.116925249119284 34.0 31.0 34.0 30.0 34.0 3 32.18844239776878 34.0 31.0 34.0 30.0 34.0 4 35.706644187294046 37.0 35.0 37.0 35.0 37.0 5 35.72961084521357 37.0 35.0 37.0 35.0 37.0 6 35.758741139651555 37.0 36.0 37.0 35.0 37.0 7 35.702690557966825 37.0 35.0 37.0 35.0 37.0 8 35.681826309690514 37.0 35.0 37.0 33.0 37.0 9 37.352419899818166 39.0 37.0 39.0 34.0 39.0 10-11 37.27960093695016 39.0 37.0 39.0 34.0 39.0 12-13 37.18637211257335 39.0 37.0 39.0 34.0 39.0 14-15 38.44250304408171 40.0 38.0 41.0 34.0 41.0 16-17 38.368731571984696 40.0 38.0 41.0 34.0 41.0 18-19 38.32357446795335 40.0 38.0 41.0 33.5 41.0 20-21 38.22417639495576 40.0 38.0 41.0 33.5 41.0 22-23 38.07968507978087 40.0 38.0 41.0 33.0 41.0 24-25 38.028553409023175 40.0 38.0 41.0 33.0 41.0 26-27 38.03361513827833 40.0 38.0 41.0 33.0 41.0 28-29 37.9437011311676 40.0 38.0 41.0 33.0 41.0 30-31 37.74684445032747 40.0 37.5 41.0 33.0 41.0 32-33 37.519170941540466 40.0 37.0 41.0 32.5 41.0 34-35 37.31430404899636 40.0 37.0 41.0 31.5 41.0 36-37 37.09457205204006 40.0 36.5 41.0 31.0 41.0 38-39 36.84771753863255 40.0 36.0 41.0 30.5 41.0 40-41 36.61034747815344 39.0 36.0 41.0 30.0 41.0 42-43 36.31489177157428 39.0 35.0 41.0 30.0 41.0 44-45 36.08048954562097 39.0 35.0 41.0 29.0 41.0 46-47 35.925007024803946 39.0 35.0 41.0 28.5 41.0 48-49 35.655799507837976 39.0 35.0 41.0 27.0 41.0 50-51 34.99062469278577 38.5 34.5 40.5 26.0 40.5 52-53 35.05019948120952 38.5 35.0 40.0 26.0 41.0 54-55 35.18312618870133 39.0 35.0 40.5 26.0 41.0 56-57 34.97221544899528 39.0 35.0 40.5 26.0 41.0 58-59 34.70865951259085 38.0 34.5 40.0 25.0 41.0 60-61 34.333740307512244 38.0 34.0 40.0 23.5 41.0 62-63 33.919279197059105 37.0 34.0 40.0 22.5 41.0 64-65 33.49853969227101 37.0 33.5 40.0 22.0 41.0 66-67 33.070969678391705 36.0 33.0 39.0 20.0 41.0 68-69 32.591288198097146 35.5 32.5 39.0 18.5 40.5 70-71 32.108366018991354 35.0 32.0 38.5 16.0 40.0 72-73 31.640034144804623 35.0 31.5 37.5 11.5 40.0 74-75 31.12776589560251 35.0 31.0 37.0 7.5 39.0 76-77 30.60336602286177 35.0 30.5 36.5 6.0 39.0 78-79 30.053405733323736 34.0 30.0 36.0 2.0 38.5 80-81 29.540435971331057 34.0 29.0 35.0 2.0 37.0 82-83 29.015377160369113 34.0 29.0 35.0 2.0 37.0 84-85 28.54718694338589 34.0 28.5 35.0 2.0 36.0 86-87 28.070131539937655 34.0 27.5 35.0 2.0 36.0 88-89 27.535923454020626 33.5 26.5 35.0 2.0 35.5 90-91 26.94613949286721 33.0 25.5 35.0 2.0 35.0 92-93 26.356168397129387 33.0 24.5 35.0 2.0 35.0 94-95 25.779726144888226 32.0 23.5 35.0 2.0 35.0 96-97 24.964405337767282 32.0 19.0 34.0 2.0 35.0 98-99 23.916360903850368 31.5 2.0 34.0 2.0 35.0 100-101 21.040808692333712 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2862688495972918 1101 2 0.3009976386454696 1101 3 0.204477453077466 1101 4 0.12840191926468236 1101 5 0.14350927186644924 1101 6 0.1312674547377739 1101 7 0.18384599233413468 1101 8 0.20313420633372203 1101 9 0.23518262054425776 1101 10-11 0.1976783398018611 1101 12-13 0.1337703018237164 1101 14-15 0.16259105935937868 1101 16-17 0.32895103302976025 1101 18-19 0.2430516655254351 1101 20-21 0.3670937769707905 1101 22-23 0.31301799530022834 1101 24-25 0.2980292345429021 1101 26-27 0.32465325326773353 1101 28-29 0.2869593031206108 1101 30-31 0.3006728249288031 1101 32-33 0.333789380360038 1101 34-35 0.3928330793330872 1101 36-37 0.3699148519037294 1101 38-39 0.3439451365914721 1101 40-41 0.44931820796651323 1101 42-43 0.39907105078543736 1101 44-45 0.3832928095761261 1101 46-47 0.540732008346744 1101 48-49 0.6711374299429522 1101 50-51 0.7834244076562555 1101 52-53 0.5400143924940508 1101 54-55 0.4808697021430959 1101 56-57 0.4581181728548316 1101 58-59 0.38878141258284415 1101 60-61 0.4025042121461553 1101 62-63 0.46285243035288204 1101 64-65 0.37669460807260435 1101 66-67 0.2501852857834237 1101 68-69 0.3349541117210961 1101 70-71 0.42530260063492165 1101 72-73 0.5352833107399704 1101 74-75 0.5485415979479527 1101 76-77 0.5621435952628282 1101 78-79 0.5669934149066229 1101 80-81 0.47982522407602346 1101 82-83 0.5308107680424534 1101 84-85 0.483536661148392 1101 86-87 0.6979325789984365 1101 88-89 0.6923543818201843 1101 90-91 0.7489301138571989 1101 92-93 0.8274674362193188 1101 94-95 0.9615154671447748 1101 96-97 1.0406605764761032 1101 98-99 1.0798088284563718 1101 100-101 0.9342810570486186 1102 1 0.46591214603025577 1102 2 0.4146282249513291 1102 3 0.31998872819021784 1102 4 0.2721284465299547 1102 5 0.22358471262085544 1102 6 0.3423605256684894 1102 7 0.23213791525336092 1102 8 0.35311247611641505 1102 9 0.303030509023138 1102 10-11 0.4056231942504027 1102 12-13 0.2781224953456558 1102 14-15 0.35848630331181397 1102 16-17 0.3421761252806945 1102 18-19 0.3680022600313819 1102 20-21 0.46373235835660864 1102 22-23 0.5105544856651321 1102 24-25 0.3039648638991963 1102 26-27 0.38718625859778655 1102 28-29 0.29877705629813534 1102 30-31 0.434023173432287 1102 32-33 0.4221505939721766 1102 34-35 0.47799093091160216 1102 36-37 0.4211641343965553 1102 38-39 0.4380638327784325 1102 40-41 0.6205666704511401 1102 42-43 0.6365919609945365 1102 44-45 0.6431683733317612 1102 46-47 0.572124792274586 1102 48-49 0.4960345189138451 1102 50-51 0.8066738951511354 1102 52-53 0.7341765491156096 1102 54-55 0.6857885213312827 1102 56-57 0.7808772954460537 1102 58-59 0.7047344671133899 1102 60-61 0.6780771928759606 1102 62-63 0.7026894537231243 1102 64-65 0.6627575437019502 1102 66-67 0.872044244373015 1102 68-69 0.7902716598965824 1102 70-71 0.6989618872277958 1102 72-73 0.754016193068793 1102 74-75 0.7176658291902669 1102 76-77 0.6152163709905842 1102 78-79 0.7070301102735748 1102 80-81 0.811479795621743 1102 82-83 0.8517619775545491 1102 84-85 0.824418989971683 1102 86-87 0.7467739074117219 1102 88-89 0.7888578668550323 1102 90-91 1.0643686132421948 1102 92-93 1.2145082316272742 1102 94-95 1.1511384683747856 1102 96-97 1.209993089801241 1102 98-99 1.3319666800348884 1102 100-101 1.406981549053537 1103 1 0.24412555978869932 1103 2 0.27572641059928316 1103 3 0.19995751559028463 1103 4 0.12075097513353228 1103 5 0.14792268954651178 1103 6 0.10067083252855724 1103 7 0.18618147885143088 1103 8 0.20998585444951345 1103 9 0.19428159811309342 1103 10-11 0.2012697067689544 1103 12-13 0.1692877019137029 1103 14-15 0.2311692464876458 1103 16-17 0.16034329460425312 1103 18-19 0.17973130402582882 1103 20-21 0.28861799194366 1103 22-23 0.3128521542945535 1103 24-25 0.2651788042505814 1103 26-27 0.12074612264106577 1103 28-29 0.27605284738436353 1103 30-31 0.38388894010373775 1103 32-33 0.4625887193012943 1103 34-35 0.48950656114761415 1103 36-37 0.41931043149072167 1103 38-39 0.39677329708979414 1103 40-41 0.42792700364086045 1103 42-43 0.5781915588690225 1103 44-45 0.47778352963412374 1103 46-47 0.5259621229270834 1103 48-49 0.5746106115941956 1103 50-51 0.4925890658151175 1103 52-53 0.5536114670149885 1103 54-55 0.6854403955234432 1103 56-57 0.5892634316900143 1103 58-59 0.6162897354364318 1103 60-61 0.5568741673601352 1103 62-63 0.5585633586491596 1103 64-65 0.5211270204600638 1103 66-67 0.5375900340230757 1103 68-69 0.571619163367302 1103 70-71 0.7506838461535423 1103 72-73 0.7947241254734081 1103 74-75 0.65506378983941 1103 76-77 0.7067606287338926 1103 78-79 0.8304581345925932 1103 80-81 0.8926891402528874 1103 82-83 0.7626097921938317 1103 84-85 0.9373845699420045 1103 86-87 0.6819156566577362 1103 88-89 0.7688606434360103 1103 90-91 0.8664346767205799 1103 92-93 0.9611844727110999 1103 94-95 1.0408374486993566 1103 96-97 0.9552249343127031 1103 98-99 1.1493926937263055 1103 100-101 0.8856878538072372 1104 1 0.2921902223703441 1104 2 0.16074553697190908 1104 3 0.17411816643375033 1104 4 0.10849627529299255 1104 5 0.15591414167786155 1104 6 0.05780841506263812 1104 7 0.03435297198020493 1104 8 0.06994183627442396 1104 9 0.011496633684387803 1104 10-11 0.18366003224590344 1104 12-13 0.09765076689955521 1104 14-15 0.25727763122509373 1104 16-17 0.34778753495410797 1104 18-19 0.33124749451761915 1104 20-21 0.2898452608415596 1104 22-23 0.2651612561396135 1104 24-25 0.3371889693605965 1104 26-27 0.3282201109702143 1104 28-29 0.42995366738628604 1104 30-31 0.25566636850830804 1104 32-33 0.23725178362813892 1104 34-35 0.33024622465098474 1104 36-37 0.36616169996543135 1104 38-39 0.3330433008781952 1104 40-41 0.34364094356675423 1104 42-43 0.4346024336416434 1104 44-45 0.4953186650041701 1104 46-47 0.3157200336507273 1104 48-49 0.37413217175478053 1104 50-51 0.2894949874298476 1104 52-53 0.33652664787084063 1104 54-55 0.35911964989680456 1104 56-57 0.37942315920977876 1104 58-59 0.35628203038563555 1104 60-61 0.31389594258888565 1104 62-63 0.3378761699970596 1104 64-65 0.4284421700397445 1104 66-67 0.38893616481764326 1104 68-69 0.4624664143864905 1104 70-71 0.4735188666958905 1104 72-73 0.4001634531854634 1104 74-75 0.30107191986134296 1104 76-77 0.34550675728428004 1104 78-79 0.3122127844731999 1104 80-81 0.4707603982582782 1104 82-83 0.5341743610004173 1104 84-85 0.5405083749333279 1104 86-87 0.6472269871967562 1104 88-89 0.8199562086859089 1104 90-91 0.7868509062007156 1104 92-93 0.7334545363445599 1104 94-95 0.8377976939500904 1104 96-97 0.7719319993598255 1104 98-99 0.7986915893178796 1104 100-101 0.8155316386222289 1105 1 0.2600156358016221 1105 2 0.22199284588995738 1105 3 0.14426309642126256 1105 4 0.1252419643799385 1105 5 0.10555502950813889 1105 6 0.1643934177663624 1105 7 0.15191007730711448 1105 8 0.06937240391425092 1105 9 0.14191057320284273 1105 10-11 0.2101237959351252 1105 12-13 0.1182147536689584 1105 14-15 0.17984129729496345 1105 16-17 0.21132093073596536 1105 18-19 0.3176195885512314 1105 20-21 0.3384744361744936 1105 22-23 0.2135837676547041 1105 24-25 0.3128802269156381 1105 26-27 0.2458240443980273 1105 28-29 0.2890369840959721 1105 30-31 0.28621009681776144 1105 32-33 0.28983771791921953 1105 34-35 0.257112471224481 1105 36-37 0.3766338495991235 1105 38-39 0.34686206783659657 1105 40-41 0.21228347751090837 1105 42-43 0.18285670227011508 1105 44-45 0.244898016332165 1105 46-47 0.2704384705738292 1105 48-49 0.0743786825720889 1105 50-51 0.07917048560440776 1105 52-53 0.09535264217618789 1105 54-55 0.15295097620492726 1105 56-57 0.22680027980224793 1105 58-59 0.2644560876508919 1105 60-61 0.29260988441225777 1105 62-63 0.20387276700029844 1105 64-65 0.15684498854752604 1105 66-67 0.2334890899406048 1105 68-69 0.13773094589738122 1105 70-71 0.26536381261888664 1105 72-73 0.3039865099560757 1105 74-75 0.20752623833587336 1105 76-77 0.19588865338127803 1105 78-79 0.23063861408424557 1105 80-81 0.25148541134576874 1105 82-83 0.22015347374387773 1105 84-85 0.22614622623321523 1105 86-87 0.4856074413066267 1105 88-89 0.4372678147090241 1105 90-91 0.48171558476245124 1105 92-93 0.576457690111031 1105 94-95 0.8448464507253668 1105 96-97 0.7774523758020422 1105 98-99 0.6541263270465194 1105 100-101 0.6254540721181208 1106 1 0.14922570870325558 1106 2 0.12411023365041984 1106 3 0.09276373807554705 1106 4 0.12607704281307264 1106 5 0.11344473634564878 1106 6 0.1473364054628732 1106 7 0.2911240757911244 1106 8 0.11013422384279181 1106 9 0.23018906971906716 1106 10-11 -0.35046221500226693 1106 12-13 -0.18587513217510576 1106 14-15 -0.05562441238608784 1106 16-17 0.13345093745941483 1106 18-19 0.06859063488310113 1106 20-21 0.05542709936095491 1106 22-23 -0.20132530872000132 1106 24-25 -0.04131864737007618 1106 26-27 0.025838690389008434 1106 28-29 -0.21270811570660442 1106 30-31 -0.01409624135616383 1106 32-33 0.029241143596529184 1106 34-35 -0.10898020392429686 1106 36-37 -0.23361720210008485 1106 38-39 -0.06814336374193175 1106 40-41 0.15554096859270317 1106 42-43 -0.0012830884321104463 1106 44-45 0.10542064695769682 1106 46-47 0.2655957294038913 1106 48-49 0.2665509285619265 1106 50-51 0.3876948520510908 1106 52-53 0.2794260548491039 1106 54-55 -0.009344732919409182 1106 56-57 -0.1354303360288327 1106 58-59 0.17992545169227725 1106 60-61 -0.14039255801371553 1106 62-63 0.01579740152857312 1106 64-65 0.03851080586090916 1106 66-67 -0.11057892684090831 1106 68-69 -0.39863779047906434 1106 70-71 -0.17129513135662933 1106 72-73 0.005826394729531614 1106 74-75 0.0758572091823595 1106 76-77 0.28324642239430275 1106 78-79 0.3199870102340334 1106 80-81 0.2562018201373597 1106 82-83 0.17370741053201044 1106 84-85 0.15700815564981596 1106 86-87 0.19237928503133261 1106 88-89 0.012684675870453077 1106 90-91 0.052877114626141974 1106 92-93 0.18841886824253962 1106 94-95 0.15831205477256916 1106 96-97 0.299407087428758 1106 98-99 0.10809637556672058 1106 100-101 -0.22166376529996157 1107 1 0.21141636858366297 1107 2 0.17137271907287754 1107 3 0.13783406682244248 1107 4 -0.001016776189032953 1107 5 0.14328929947298263 1107 6 0.15950641074879712 1107 7 0.08916987418696465 1107 8 0.1315572517415191 1107 9 0.108212492795289 1107 10-11 0.1706935701404504 1107 12-13 0.0970163519444256 1107 14-15 0.11508905211128706 1107 16-17 0.20926405374567025 1107 18-19 0.23880173014232042 1107 20-21 0.3096730770186298 1107 22-23 0.38734219886267596 1107 24-25 0.34937354083246674 1107 26-27 0.39739606154529383 1107 28-29 0.37301765904693696 1107 30-31 0.38800495849604033 1107 32-33 0.3825806320579517 1107 34-35 0.383633421390158 1107 36-37 0.2221167755623128 1107 38-39 0.4085774939806228 1107 40-41 0.41845539374970286 1107 42-43 0.4261637444023876 1107 44-45 0.4054636208893072 1107 46-47 0.46782490818781497 1107 48-49 0.32220209632731667 1107 50-51 0.3664987005824969 1107 52-53 0.32250259647444324 1107 54-55 0.42426393116734573 1107 56-57 0.3947087244905987 1107 58-59 0.34855430483487027 1107 60-61 0.3632800410296255 1107 62-63 0.34051856450304996 1107 64-65 0.4305551923192823 1107 66-67 0.4500473905892761 1107 68-69 0.4547637943559195 1107 70-71 0.5507452950767728 1107 72-73 0.576693457516555 1107 74-75 0.5741869419572794 1107 76-77 0.7561863991409403 1107 78-79 0.5446005689587885 1107 80-81 0.521544707030916 1107 82-83 0.4686190731001716 1107 84-85 0.5509114556116899 1107 86-87 0.4973163813312418 1107 88-89 0.4863165520678159 1107 90-91 0.6113810325169098 1107 92-93 0.6715905300210494 1107 94-95 0.8056337490503935 1107 96-97 0.7622903244427341 1107 98-99 0.5171441928681375 1107 100-101 0.6221117030525427 1108 1 0.20767174949990164 1108 2 0.15466126914794387 1108 3 0.128477782300088 1108 4 0.04493472492731598 1108 5 0.11748971055559565 1108 6 0.17041339638306852 1108 7 0.22339111640990694 1108 8 0.1467894690945286 1108 9 0.21233079815986144 1108 10-11 0.26832042679893675 1108 12-13 0.050592549042470125 1108 14-15 0.10538262714494095 1108 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-0.7836786906783431 2316 64-65 -0.7934168752871003 2316 66-67 -0.8963869103759023 2316 68-69 -1.0323377486789127 2316 70-71 -0.9800284926194749 2316 72-73 -0.9073323473788228 2316 74-75 -0.9672412421178755 2316 76-77 -0.8790284635493997 2316 78-79 -0.9178035040607817 2316 80-81 -0.7267342941002326 2316 82-83 -0.9045043452990988 2316 84-85 -0.9687028322373159 2316 86-87 -0.9633349887501339 2316 88-89 -0.9389183240934287 2316 90-91 -0.9880536817097081 2316 92-93 -1.0334959999175695 2316 94-95 -1.1064501461248675 2316 96-97 -1.256859764838584 2316 98-99 -1.287885480367109 2316 100-101 -1.1601203055532245 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6235.0 3 1674.0 4 1703.0 5 1936.0 6 2351.0 7 2873.0 8 3497.0 9 4503.0 10 5687.0 11 7228.0 12 9261.0 13 11676.0 14 13870.0 15 17233.0 16 21171.0 17 28105.0 18 25124.0 19 19730.0 20 19100.0 21 19326.0 22 20571.0 23 22286.0 24 24448.0 25 27402.0 26 31334.0 27 36366.0 28 42591.0 29 50526.0 30 60582.0 31 74782.0 32 92996.0 33 121497.0 34 167141.0 35 248836.0 36 375302.0 37 519859.0 38 396421.0 39 48475.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06662715026036 18.323672038719856 14.504776973447775 23.10492383757201 2 15.58501404318711 19.664334332020207 40.35055109780045 24.400100526992233 3 19.438468762404515 23.764389237963385 29.444297069387833 27.352844930244263 4 12.717410669331288 16.50187065740246 36.752724653173146 34.027994020093104 5 13.695076026082909 37.305615789192345 34.034367647525926 14.964940537198823 6 32.53125300286105 35.924331001304985 17.052724815488716 14.491691180345248 7 29.00422253396967 32.92879325076243 19.909236047472714 18.15774816779518 8 26.86590225856532 34.30399143710091 19.338977649021047 19.49112865531272 9 26.566649550527078 16.77536577035407 19.992541700259995 36.665442978858856 10-11 25.148417403928125 26.503337479117757 26.690440048355 21.65780506859911 12-13 26.29797215824729 23.89426906874886 27.187826440055918 22.619932332947933 14-15 22.85875938569923 25.251224415268368 25.894577332275343 25.995438866757052 16-17 22.113095762534797 28.456619876330375 26.807898079157006 22.622386281977825 18-19 22.163022959008313 26.59524802146122 29.421057024091013 21.82067199543945 20-21 22.557558219591282 25.78261187010156 29.945457654684958 21.71437225562219 22-23 22.342685008286107 25.776723225458387 29.81249658699172 22.068095179263782 24-25 22.09091874042664 26.052653191197585 29.852730049252685 22.00369801912309 26-27 21.586990764068364 26.40792089484779 29.706860739873765 22.29822760121008 28-29 21.861710697747917 26.84533943927956 29.493575433274827 21.799374429697703 30-31 21.840006909776676 26.279034908703025 29.43619088490458 22.44476729661572 32-33 21.457195626480402 26.31329432910001 29.54296646618431 22.686543578235273 34-35 22.39278043191683 26.507538393995077 29.184374644751088 21.915306529337002 36-37 22.519508159101758 25.964505268824805 29.188234006892124 22.327752565181314 38-39 21.914589953640586 26.382118810662085 29.172957047556245 22.530334188141083 40-41 22.483847841057838 26.218587484963983 29.2330698792027 22.06449479477547 42-43 22.10679915967639 26.236144408500582 29.01271269215065 22.644343739672376 44-45 22.057333081441175 26.55691807878547 29.276675921638827 22.10907291813453 46-47 22.59471764733211 26.92218944303778 28.330565169922643 22.152527739707466 48-49 22.28428324838174 26.93260764906987 28.27812786313589 22.50498123941251 50-51 22.48381428168241 27.130759852837045 28.29472235051899 22.090703514961554 52-53 22.811460879213538 26.81484762175026 28.22449983797991 22.149191661056296 54-55 22.250614979245174 27.037426045697003 28.429849780140565 22.282109194917254 56-57 22.41954781583951 27.14725146585971 28.139686543823068 22.293514174477714 58-59 22.362420326808884 26.69412172555742 28.17739947710733 22.766058470526364 60-61 21.969620711869865 27.028828344710533 28.509019165727175 22.49253177769243 62-63 22.140251257759864 26.944669405426836 28.682305885340615 22.23277345147269 64-65 21.840859260962635 26.958830724359284 28.5234021788132 22.67690783586488 66-67 21.855040395592475 27.063546919024358 28.39724697180641 22.684165713576757 68-69 22.4287003376023 26.60879825426977 28.53900775660736 22.423493651520566 70-71 22.172322392951184 26.7870573993023 28.162850997930978 22.87776920981554 72-73 22.16396561700882 26.99768713770175 28.113889544418203 22.724457700871227 74-75 22.243019456415922 27.353757469146306 27.947533669354176 22.455689405083596 76-77 22.44647115219155 26.923969553831583 28.014125572733555 22.61543372124331 78-79 22.43282445052967 27.153558269906856 27.98005733738086 22.433559942182615 80-81 22.66533965637479 27.213988732412904 27.677339073388353 22.443332537823952 82-83 22.58081312521292 27.37716404967636 27.680847192542352 22.36117563256837 84-85 22.56282538228093 27.450675254426514 27.671582446888305 22.31491691640425 86-87 22.731473408386556 27.457836993902156 27.378002059644537 22.432687538066748 88-89 22.564890373166836 27.502076375780454 27.249871739025217 22.683161512027493 90-91 22.607704973643976 27.482794621295792 27.294999248741057 22.61450115631917 92-93 22.761804189021895 27.068042809755603 27.571135840732374 22.599017160490124 94-95 22.57514618750726 26.869438097819774 27.727103744723696 22.82831196994927 96-97 22.913617390361576 26.518539599934947 27.662318301853183 22.90552470785029 98-99 22.577489704111038 26.790663873344982 27.31584422804934 23.316002194494637 100-101 23.128686524038525 26.23191375241988 27.547911606969265 23.091488116572325 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 993.0 1 686.0 2 556.0 3 747.0 4 629.5 5 582.5 6 1098.0 7 2378.5 8 3977.0 9 4582.5 10 3773.0 11 2925.5 12 2817.0 13 2889.0 14 2969.5 15 3191.5 16 3495.5 17 3738.0 18 4001.5 19 4405.5 20 4919.0 21 5730.5 22 6822.0 23 8243.5 24 10222.5 25 13062.5 26 16711.5 27 20497.5 28 23744.5 29 27087.0 30 30809.0 31 36143.0 32 45029.0 33 49142.5 34 49340.5 35 52586.0 36 57737.0 37 62894.5 38 65803.5 39 68901.0 40 71982.5 41 75570.5 42 78335.5 43 80361.5 44 82991.0 45 87866.0 46 91259.0 47 93242.0 48 93701.0 49 91251.5 50 90443.5 51 88176.5 52 86866.5 53 88739.5 54 91373.5 55 87425.0 56 79441.0 57 72856.5 58 68024.0 59 60347.0 60 48070.0 61 39308.5 62 33153.0 63 25120.5 64 17754.5 65 13711.0 66 10384.5 67 7773.5 68 5915.0 69 4550.5 70 3519.0 71 2410.5 72 1710.0 73 1379.0 74 1042.5 75 722.5 76 520.0 77 340.0 78 215.5 79 148.5 80 98.0 81 64.5 82 44.0 83 31.5 84 24.5 85 16.5 86 10.5 87 8.0 88 7.5 89 4.0 90 3.0 91 3.5 92 5.5 93 5.0 94 1.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4883690146928709 2 0.05074114584421888 3 0.057166035401915546 4 0.06664855312261243 5 0.08081427347271473 6 0.1095327557125396 7 0.07121564329013176 8 0.05414711139287735 9 0.0520183829249658 10-11 0.07597625422746121 12-13 0.1095327557125396 14-15 0.05608231909097876 16-17 0.06117191533698546 18-19 0.060243015641896776 20-21 0.07533763568708775 22-23 0.06459723296262494 24-25 0.08979363719190527 26-27 0.06660984896865041 28-29 0.06726781958600489 30-31 0.07171879729163812 32-33 0.07142851613692291 34-35 0.07297668229540404 36-37 0.06848700043580877 38-39 0.040832882429939675 40-41 0.04460653744123742 42-43 0.04356152528426266 44-45 0.022680634221748486 46-47 0.029492565319065432 48-49 0.029434509088122392 50-51 0.027673470082850114 52-53 0.02666716207983738 54-55 0.026628457925875353 56-57 0.025602797845881608 58-59 0.022119423989299076 60-61 0.02933774870321732 62-63 0.020822834831571133 64-65 0.023164436146273837 66-67 0.016933067358387306 68-69 0.018771514671583642 70-71 0.041935950817857476 72-73 0.03760108557411033 74-75 0.03198898324961624 76-77 0.02266128214476747 78-79 0.01532684496896314 80-81 0.0174749255138557 82-83 0.02252581760590037 84-85 0.018984387518374796 86-87 0.009540573951639934 88-89 0.04168437381710429 90-91 0.053024690927978534 92-93 0.03404030340960374 94-95 0.05426322385476344 96-97 0.04342606074539556 98-99 0.03386613471677461 100-101 0.16606017257408168 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2583702.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.676714457491826 #Duplication Level Percentage of deduplicated Percentage of total 1 75.38835744103719 39.712109783408444 2 13.859936151332503 14.601917980856207 3 4.717833770899431 7.455599472227438 4 2.05088129366849 4.321347531711459 5 1.0742049443447375 2.8292793561036818 6 0.6497378729790839 2.0535633844282373 7 0.40887985257377346 1.5076913069015014 8 0.2879100139185435 1.213292287411169 9 0.2133849148368264 1.0116374605556142 >10 1.2001485776858623 11.685977091112587 >50 0.09269050263896893 3.3239525663198237 >100 0.049686969910709755 4.847499827163912 >500 0.0028786054974917485 1.1116824715940452 >1k 0.0032476574843475397 3.1808846769563983 >5k 7.3810397371535E-5 0.24875994870087195 >10k+ 1.4762079474307E-4 0.8948048545485883 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12984 0.5025347350429732 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10030 0.3882026642391421 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6398 0.24762917704905596 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4023 0.15570681138923917 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3710 0.14359241119912436 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3562 0.1378641964127442 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3415 0.13217468578032607 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3163 0.12242123898189496 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2819 0.1091070100189573 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2810 0.10875867263329905 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2766 0.1070556898589698 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.870415396202813E-5 2 0.0 0.0 0.0 0.0 3.870415396202813E-5 3 0.0 0.0 0.0 0.0 3.870415396202813E-5 4 0.0 0.0 0.0 0.0 3.870415396202813E-5 5 0.0 0.0 0.0 0.0 7.740830792405626E-5 6 0.0 0.0 0.0 0.0 7.740830792405626E-5 7 0.0 0.0 0.0 3.870415396202813E-5 7.740830792405626E-5 8 0.0 0.0 0.0 3.870415396202813E-5 7.740830792405626E-5 9 0.0 0.0 0.0 3.870415396202813E-5 7.740830792405626E-5 10-11 0.0 0.0 0.0 7.740830792405626E-5 7.740830792405626E-5 12-13 0.0 0.0 0.0 1.1611246188608438E-4 1.1611246188608438E-4 14-15 0.0 0.0 0.0 1.1611246188608438E-4 1.7416869282912658E-4 16-17 0.0 0.0 0.0 1.3546453886709845E-4 1.9352076981014063E-4 18-19 0.0 0.0 0.0 2.1287284679115468E-4 2.3222492377216876E-4 20-21 0.0 0.0 0.0 2.9028115471521095E-4 2.709290777341969E-4 22-23 0.0 0.0 0.0 4.644498475443376E-4 3.4833738565825316E-4 24-25 0.0 0.0 0.0 6.579706173544782E-4 3.8704153962028126E-4 26-27 0.0 0.0 0.0 0.0012965891577279422 3.8704153962028126E-4 28-29 0.0 0.0 0.0 0.0032317968558293487 3.8704153962028126E-4 30-31 0.0 0.0 0.0 0.010604938185595707 3.8704153962028126E-4 32-33 0.0 0.0 0.0 0.029279692472274278 3.8704153962028126E-4 34-35 0.0 0.0 0.0 0.055888798321168616 3.8704153962028126E-4 36-37 0.0 0.0 0.0 0.10055339199334908 3.8704153962028126E-4 38-39 0.0 0.0 0.0 0.16900168827519582 4.063936166012953E-4 40-41 0.0 0.0 0.0 0.238049898943454 4.257456935823094E-4 42-43 0.0 0.0 0.0 0.3101170336207504 4.257456935823094E-4 44-45 0.0 0.0 0.0 0.39472431418174386 4.450977705633235E-4 46-47 0.0 0.0 0.0 0.49369083586264983 4.644498475443375E-4 48-49 0.0 0.0 0.0 0.5975727850967334 5.225060784873797E-4 50-51 0.0 0.0 0.0 0.7112855894371719 5.418581554683938E-4 52-53 0.0 0.0 0.0 0.818708968758781 5.999143864114361E-4 54-55 0.0 0.0 0.0 0.9337183622569476 6.192664633924501E-4 56-57 0.0 0.0 0.0 1.0611324370999442 6.386185403734642E-4 58-59 0.0 0.0 0.0 1.199732012437967 6.579706173544782E-4 60-61 0.0 0.0 0.0 1.353677784821934 6.579706173544782E-4 62-63 0.0 0.0 0.0 1.4973476043289822 6.966747713165063E-4 64-65 0.0 0.0 0.0 1.645661922311474 7.160268482975204E-4 66-67 0.0 0.0 0.0 1.8207401627587083 7.934351562215766E-4 68-69 0.0 0.0 0.0 2.01236442902471 8.127872332025907E-4 70-71 0.0 0.0 0.0 2.2107812743110467 8.127872332025907E-4 72-73 0.0 0.0 0.0 2.4141135471505617 8.90195541126647E-4 74-75 0.0 0.0 0.0 2.6098598058135187 0.0010256600799937454 76-77 0.0 0.0 0.0 2.8228100609125972 0.0010450121569747595 78-79 0.0 0.0 0.0 3.0692200571118495 0.0010450121569747595 80-81 0.0 0.0 0.0 3.3270284266529186 0.0010450121569747595 82-83 0.0 0.0 0.0 3.589365182207546 0.0011611246188608438 84-85 0.0 0.0 0.0 3.8512955441455707 0.0011611246188608438 86-87 0.0 0.0 0.0 4.131784547908389 0.0011804766958418578 88-89 0.0 0.0 0.0 4.423013954395669 0.001199828772822872 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5310 0.0 56.38339 1 GTATCAA 10135 0.0 44.47607 1 ATCAACG 12930 0.0 34.3984 3 TATCAAC 13015 0.0 34.137234 2 TCAACGC 13140 0.0 33.81249 4 CAACGCA 13320 0.0 33.355568 5 AACGCAG 13680 0.0 32.408314 6 CGCAGAG 15610 0.0 28.202513 8 ACGCAGA 15665 0.0 28.133844 7 TGGTATC 2420 0.0 27.293568 2 GTGGTAT 2620 0.0 26.791325 1 TAAGGTG 1760 0.0 26.459053 5 GCAGAGT 17100 0.0 25.271969 9 TACATGG 9605 0.0 25.033064 2 GTACATG 10090 0.0 24.703444 1 GTAAGGT 1840 0.0 23.75915 4 GAGTACT 10825 0.0 23.64691 12-13 ACATGGG 9890 0.0 23.206612 3 GTGTAAG 1085 0.0 22.444946 1 AGGTAAG 1820 0.0 22.192612 2 >>END_MODULE