FastQCFastQC Report
Thu 26 May 2016
SRR938261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583702
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124770.482911728984225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102080.3950920036443832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65150.2521575630626133No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41390.16019649324883445No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37870.14657263105420051No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35990.13929625010933924No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC34320.13283265639768055No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34280.13267783978183242No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28850.11166148418045115No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28560.11053906371555233No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA27860.10782977293821037No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27230.1053914112386026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54950.057.4917561
GTATCAA100800.041.80371
ATCAACG126100.033.147593
TCAACGC128600.032.5013124
TATCAAC129650.032.28162
CAACGCA130400.032.016255
AACGCAG134850.030.994956
TGGTATC23100.029.4086932
GTGGTAT25750.028.4115641
ACGCAGA153950.027.0878187
CGCAGAG155800.026.7052028
TAAGGTG15700.024.5044295
GCAGAGT173550.023.6728579
TACATGG97200.023.11782
GTACATG103250.023.0515081
GAGTACT108050.022.81401812-13
GTAAGGT15200.022.8106924
GGACCGA6950.022.552176
CAGAGTA165000.021.68994910-11
GTACTTT112650.021.67160814-15
Produced by FastQC (version 0.11.5)