##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938261_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2583702 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51354490572055 34.0 31.0 34.0 31.0 34.0 2 32.73077584024783 34.0 31.0 34.0 31.0 34.0 3 32.78756799352247 34.0 31.0 34.0 31.0 34.0 4 36.24421005208805 37.0 35.0 37.0 35.0 37.0 5 36.20790865200399 37.0 37.0 37.0 35.0 37.0 6 36.24497562025342 37.0 37.0 37.0 35.0 37.0 7 36.21281866097561 37.0 37.0 37.0 35.0 37.0 8 36.20534295363784 37.0 37.0 37.0 35.0 37.0 9 37.91800408870682 39.0 38.0 39.0 35.0 39.0 10-11 37.905229976212425 39.0 38.0 39.0 35.0 39.0 12-13 37.85519672934417 39.0 38.0 39.0 35.0 39.0 14-15 39.17730198761312 40.5 39.0 41.0 36.0 41.0 16-17 39.13621694762012 40.0 38.5 41.0 36.0 41.0 18-19 39.1698227969015 40.0 39.0 41.0 36.0 41.0 20-21 39.099101792699 40.0 39.0 41.0 36.0 41.0 22-23 38.95716417760252 40.0 38.0 41.0 35.0 41.0 24-25 38.91515933339062 40.0 38.0 41.0 35.0 41.0 26-27 38.878384775024365 40.0 38.0 41.0 35.0 41.0 28-29 38.81985287002913 40.0 38.0 41.0 35.0 41.0 30-31 38.61836659181283 40.0 38.0 41.0 34.5 41.0 32-33 38.43675296144834 40.0 38.0 41.0 34.0 41.0 34-35 38.23724930351875 40.0 38.0 41.0 34.0 41.0 36-37 38.099476642430126 40.0 38.0 41.0 34.0 41.0 38-39 38.07060934271831 40.0 38.0 41.0 33.5 41.0 40-41 38.00217130303727 40.0 38.0 41.0 33.0 41.0 42-43 37.83867528066317 40.0 37.5 41.0 33.0 41.0 44-45 37.62038559400426 40.0 37.0 41.0 33.0 41.0 46-47 37.2901389169494 40.0 37.0 41.0 32.5 41.0 48-49 36.935277172057766 40.0 36.5 41.0 31.0 41.0 50-51 36.63500434647649 40.0 36.0 41.0 31.0 41.0 52-53 36.22004530708263 39.0 35.0 41.0 30.0 41.0 54-55 35.99096761158988 39.0 35.0 41.0 29.0 41.0 56-57 35.74408697287845 39.0 35.0 40.5 29.0 41.0 58-59 35.407677820429754 38.5 35.0 40.0 28.0 41.0 60-61 35.05211340162294 38.0 34.5 40.0 27.5 41.0 62-63 34.68963642091851 37.5 34.0 40.0 26.5 41.0 64-65 34.30303262527954 37.0 34.0 40.0 26.0 41.0 66-67 33.88320228106802 36.0 34.0 39.0 26.0 41.0 68-69 33.431332638206726 36.0 33.0 39.0 25.0 40.5 70-71 32.915870135178125 35.0 32.5 38.5 23.5 40.0 72-73 32.50656867548967 35.0 32.0 37.5 22.5 40.0 74-75 32.06345720210767 35.0 32.0 37.0 21.5 39.0 76-77 30.756047911098108 34.0 30.5 35.5 20.5 38.5 78-79 31.47599452258813 35.0 31.5 36.0 21.0 38.5 80-81 31.32246230408925 35.0 32.0 36.0 20.0 37.5 82-83 30.960373139007515 35.0 31.0 35.0 19.5 37.0 84-85 30.588055046595933 34.0 31.0 35.0 17.5 36.5 86-87 30.174502516157048 34.0 31.0 35.0 12.5 36.0 88-89 29.715867000141657 34.0 30.0 35.0 7.0 36.0 90-91 29.38107664893242 34.0 30.0 35.0 2.0 35.0 92-93 28.9846894107757 34.0 29.0 35.0 2.0 35.0 94-95 28.5878437219153 34.0 29.0 35.0 2.0 35.0 96-97 28.024068758703596 34.0 29.0 35.0 2.0 35.0 98-99 27.353617986904062 33.5 27.0 35.0 2.0 35.0 100-101 24.91314594330151 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17827315125823873 1101 2 0.18465307071978998 1101 3 0.13456332731549026 1101 4 0.03926314035494727 1101 5 0.09547821603275253 1101 6 0.06960901305093614 1101 7 0.14122234941132206 1101 8 0.08155954545128452 1101 9 0.06614473738184046 1101 10-11 0.060422286444456574 1101 12-13 0.14023303290201738 1101 14-15 0.13046658581560422 1101 16-17 0.17717402730250598 1101 18-19 0.22693421478025755 1101 20-21 0.18747973105534754 1101 22-23 0.29345675869677024 1101 24-25 0.31437533731450884 1101 26-27 0.31155652651504795 1101 28-29 0.2503241371290983 1101 30-31 0.1447014084599587 1101 32-33 0.19546152991226506 1101 34-35 0.1820763897219564 1101 36-37 0.23989127408405153 1101 38-39 0.23580540475299472 1101 40-41 0.3702953069725652 1101 42-43 0.2355069777235883 1101 44-45 0.2711910715142736 1101 46-47 0.2041760488736628 1101 48-49 0.28011866039279454 1101 50-51 0.24644810591950517 1101 52-53 0.18167690922609836 1101 54-55 0.15249422780713928 1101 56-57 0.25410225330022485 1101 58-59 0.2001328561406055 1101 60-61 0.2565566581535137 1101 62-63 0.26710104950449676 1101 64-65 0.36386379691683857 1101 66-67 0.3795789821428244 1101 68-69 0.4125120695681659 1101 70-71 0.2629992736187461 1101 72-73 0.20454900411654364 1101 74-75 0.1439536661410017 1101 76-77 0.2086223714712503 1101 78-79 0.19867467826734497 1101 80-81 0.3260824812032439 1101 82-83 0.2733880105504092 1101 84-85 0.36982142211594393 1101 86-87 0.4142628345676229 1101 88-89 0.3645926751423474 1101 90-91 0.34227195946862565 1101 92-93 0.5003399786786211 1101 94-95 0.45516245185029547 1101 96-97 0.46510423059459427 1101 98-99 0.43959539011369486 1101 100-101 0.49575328011304265 1102 1 0.18922395076623388 1102 2 0.21623896657875008 1102 3 0.14733126499486104 1102 4 0.10178753559890197 1102 5 0.17895669081750043 1102 6 0.16234415040231198 1102 7 0.15276396482747856 1102 8 0.1410474503303334 1102 9 0.20383846431911223 1102 10-11 0.1799247464690481 1102 12-13 0.2061684572562612 1102 14-15 0.27071053825512337 1102 16-17 0.24814717203394565 1102 18-19 0.23757341117222097 1102 20-21 0.2254951062091024 1102 22-23 0.3081670908000902 1102 24-25 0.29679354149654813 1102 26-27 0.15507682171800496 1102 28-29 0.16156521953992353 1102 30-31 0.2890913223590914 1102 32-33 0.3583192584895514 1102 34-35 0.39987405269858556 1102 36-37 0.3785308804801133 1102 38-39 0.26018383853732985 1102 40-41 0.4956237202566953 1102 42-43 0.36427840043781856 1102 44-45 0.38783026790623865 1102 46-47 0.3254642817559912 1102 48-49 0.32046962390242584 1102 50-51 0.2795811522499676 1102 52-53 0.31653525280953687 1102 54-55 0.3364153020178833 1102 56-57 0.5291084033617253 1102 58-59 0.3885340451524897 1102 60-61 0.3584885974729133 1102 62-63 0.4415948994429968 1102 64-65 0.6153767120459861 1102 66-67 0.4881779981329899 1102 68-69 0.6089778342258114 1102 70-71 0.6143682773087846 1102 72-73 0.5065479791062941 1102 74-75 0.5259573561779121 1102 76-77 0.36922815252906105 1102 78-79 0.33571588367939853 1102 80-81 0.3009922803012337 1102 82-83 0.5494353660239639 1102 84-85 0.5911069899716246 1102 86-87 0.495756684506123 1102 88-89 0.34839853820097844 1102 90-91 0.3988750754997845 1102 92-93 0.4677590028688634 1102 94-95 0.4359638048638246 1102 96-97 0.37733444789676795 1102 98-99 0.3772462716225107 1102 100-101 0.5092500000802431 1103 1 0.12704845169973566 1103 2 0.15684464420812105 1103 3 0.10303445806298583 1103 4 0.03565358950287134 1103 5 0.07788416460753211 1103 6 0.04676389285049254 1103 7 0.13776085314189856 1103 8 0.09236077313180857 1103 9 0.05479064010540924 1103 10-11 0.08178199190923152 1103 12-13 0.11930675971209581 1103 14-15 0.17348568306818635 1103 16-17 0.11563886622706576 1103 18-19 0.1601870355678372 1103 20-21 0.20624420860749382 1103 22-23 0.2052485212180173 1103 24-25 0.2225071706793713 1103 26-27 0.132007455065704 1103 28-29 0.17845248077789932 1103 30-31 0.09711746051793568 1103 32-33 0.19729144480771055 1103 34-35 0.1560880409220502 1103 36-37 0.2159696281463468 1103 38-39 0.14291953675170532 1103 40-41 0.2970394893217545 1103 42-43 0.3647960891735167 1103 44-45 0.27833445671374335 1103 46-47 0.2863445536694371 1103 48-49 0.392566612748233 1103 50-51 0.318581391610401 1103 52-53 0.3499101391133266 1103 54-55 0.4949103951290894 1103 56-57 0.4771078288966919 1103 58-59 0.5849365536497899 1103 60-61 0.5582247265360536 1103 62-63 0.6114963786904468 1103 64-65 0.4966089395341342 1103 66-67 0.4216167457992199 1103 68-69 0.4728090372513236 1103 70-71 0.6085966271579863 1103 72-73 0.5319423014769171 1103 74-75 0.4088535216238327 1103 76-77 0.4001401157632998 1103 78-79 0.44824611490157196 1103 80-81 0.5452988531386147 1103 82-83 0.6173566643149293 1103 84-85 0.6593520950508527 1103 86-87 0.7568809125687395 1103 88-89 0.6633398948958877 1103 90-91 0.5954172747434043 1103 92-93 0.6077053861327819 1103 94-95 0.6424259619800097 1103 96-97 0.5963568041608305 1103 98-99 0.6450777618805432 1103 100-101 0.7644964934499612 1104 1 0.15899217455016412 1104 2 0.10508045295971158 1104 3 0.10954136099412892 1104 4 0.033705775459267784 1104 5 0.027220160570841756 1104 6 0.08130588849427056 1104 7 0.09991384310592366 1104 8 0.07536264026189343 1104 9 0.09525640561717097 1104 10-11 0.07787817074905234 1104 12-13 0.1238890029362949 1104 14-15 0.1438780546760583 1104 16-17 0.1113792697786593 1104 18-19 0.22700724517157767 1104 20-21 0.25611161440808417 1104 22-23 0.16319561498364266 1104 24-25 0.15584218418636198 1104 26-27 0.1359300668606238 1104 28-29 0.1843368882735632 1104 30-31 0.1381410674328194 1104 32-33 0.19936055195334035 1104 34-35 0.2537257820633343 1104 36-37 0.2772843598793173 1104 38-39 0.30230748926535966 1104 40-41 0.3311979244733152 1104 42-43 0.36477658609764063 1104 44-45 0.3510491370440789 1104 46-47 0.3767706985489738 1104 48-49 0.20986037306415284 1104 50-51 0.3272484160412503 1104 52-53 0.31126457162814347 1104 54-55 0.38234594369718167 1104 56-57 0.4442772058972082 1104 58-59 0.3108723851460624 1104 60-61 0.34780612997026594 1104 62-63 0.34100732882152585 1104 64-65 0.3556660878210991 1104 66-67 0.37978968340238595 1104 68-69 0.4464786380474095 1104 70-71 0.3818986617651703 1104 72-73 0.2897833840027175 1104 74-75 0.24781680222999114 1104 76-77 0.32580249454366594 1104 78-79 0.3476453311094687 1104 80-81 0.26557727869359127 1104 82-83 0.2146419252082019 1104 84-85 0.34236361575822016 1104 86-87 0.25051925857254176 1104 88-89 0.20589658606281347 1104 90-91 0.17165675755389387 1104 92-93 0.31242668005410934 1104 94-95 0.4520371133935228 1104 96-97 0.4214004990657543 1104 98-99 0.49305849889732656 1104 100-101 0.6057552593701239 1105 1 0.07465997162463367 1105 2 0.046300739543049474 1105 3 0.05744277722912017 1105 4 0.05555247743604497 1105 5 0.05988408752828889 1105 6 -0.0031480276963620213 1105 7 0.022880290539468717 1105 8 0.068046246960904 1105 9 -0.08217036039007297 1105 10-11 0.03193116443937072 1105 12-13 -0.019021273991228327 1105 14-15 0.09184571675492492 1105 16-17 0.06537862008850226 1105 18-19 0.04985259288100252 1105 20-21 0.05414171671853296 1105 22-23 0.10742822196736057 1105 24-25 0.15133987121976844 1105 26-27 0.10311051606216637 1105 28-29 0.0012146379113673333 1105 30-31 0.1476737531653569 1105 32-33 0.07682306915942405 1105 34-35 0.009620542871068949 1105 36-37 0.12130905938103353 1105 38-39 0.06268764920720571 1105 40-41 0.041647787645388235 1105 42-43 0.009572423702962851 1105 44-45 -0.05109712880454964 1105 46-47 0.05082970494090944 1105 48-49 0.19325662751253248 1105 50-51 0.14296342260696804 1105 52-53 0.20157977747860656 1105 54-55 0.22243535817512594 1105 56-57 0.20966195330155557 1105 58-59 0.23667844948462147 1105 60-61 0.11254234797431195 1105 62-63 0.14515045499855006 1105 64-65 0.10102452592484923 1105 66-67 0.1787411750282928 1105 68-69 0.1842245249598733 1105 70-71 0.07807946864452475 1105 72-73 0.07529807938708188 1105 74-75 0.17115029641055912 1105 76-77 0.18175923957278428 1105 78-79 0.15959394224337942 1105 80-81 0.25038658435257943 1105 82-83 0.34811005515352633 1105 84-85 0.38672030726003115 1105 86-87 0.3470154089345243 1105 88-89 0.18593163086042352 1105 90-91 0.21900744613757084 1105 92-93 0.24215932376737825 1105 94-95 0.21629112134998962 1105 96-97 0.3687287519481117 1105 98-99 0.505358586103057 1105 100-101 0.5617281388688511 1106 1 -0.11667719593206982 1106 2 -0.03902714650942585 1106 3 -0.01102223441201744 1106 4 -0.016516932570333154 1106 5 -0.004651059281357561 1106 6 0.0042120823912412675 1106 7 -4.1911148727535874E-4 1106 8 -0.05158837410620265 1106 9 -0.15245255980109107 1106 10-11 -0.04215670865155374 1106 12-13 -0.08188769141910512 1106 14-15 -0.21554094454439365 1106 16-17 -0.09156259467226135 1106 18-19 0.018014692311012936 1106 20-21 0.10590159098569529 1106 22-23 0.04125289546596633 1106 24-25 -0.02505224735279654 1106 26-27 -0.010346665826475032 1106 28-29 0.016977635514614065 1106 30-31 0.06348357226023893 1106 32-33 -0.0198183453064118 1106 34-35 -0.04176533045681197 1106 36-37 -0.16443550939731466 1106 38-39 -0.20042272532189997 1106 40-41 -0.17716631533049565 1106 42-43 -0.27808854301809305 1106 44-45 -0.30783521252080703 1106 46-47 -0.37259503022502827 1106 48-49 -0.42216620053411447 1106 50-51 -0.3463832605614172 1106 52-53 -0.40337459297931133 1106 54-55 -0.6745202456294095 1106 56-57 -0.4440588564247534 1106 58-59 -0.33955136410017417 1106 60-61 -0.32452128785726586 1106 62-63 -0.18541498588717076 1106 64-65 -0.3509498992628295 1106 66-67 -0.38840484093383765 1106 68-69 -0.49510678420044485 1106 70-71 -0.5375510583970282 1106 72-73 -0.45269203671023206 1106 74-75 -0.456645253549258 1106 76-77 -0.3342435754266546 1106 78-79 -0.3135193871786477 1106 80-81 -0.5942153116323361 1106 82-83 -0.6667022377720038 1106 84-85 -0.5483584113727815 1106 86-87 -0.34395703633214936 1106 88-89 -0.2866706591102144 1106 90-91 -0.5092822989565207 1106 92-93 -0.37160833600025356 1106 94-95 -0.4995174130088529 1106 96-97 -0.39639508946978097 1106 98-99 -0.27595578452616465 1106 100-101 -0.29569899161607793 1107 1 0.09844998156603424 1107 2 0.09482184453867859 1107 3 0.054738782795823226 1107 4 0.05042605190954674 1107 5 0.0348593237037349 1107 6 0.025615221771744245 1107 7 0.06932299777994899 1107 8 0.025197806349545715 1107 9 0.04260150455755962 1107 10-11 0.0645904195937348 1107 12-13 0.1015395451847283 1107 14-15 0.13162625277740148 1107 16-17 0.11657472626477983 1107 18-19 0.10859112933747639 1107 20-21 0.12101472866314822 1107 22-23 0.1311566586093349 1107 24-25 0.09859135570419397 1107 26-27 0.08083092007865389 1107 28-29 0.09583546295770873 1107 30-31 0.13932033378483055 1107 32-33 0.166013413150921 1107 34-35 0.22516863789481079 1107 36-37 0.23809918649104134 1107 38-39 0.20563275392067482 1107 40-41 0.3209754341166047 1107 42-43 0.2735082398152002 1107 44-45 0.3739731723606141 1107 46-47 0.4174709053691714 1107 48-49 0.5079069479627023 1107 50-51 0.4453206786616235 1107 52-53 0.47636601949276525 1107 54-55 0.3985247572257009 1107 56-57 0.25925991851323715 1107 58-59 0.36553387956216454 1107 60-61 0.3282415367012561 1107 62-63 0.346239211415174 1107 64-65 0.2962112873067895 1107 66-67 0.2241114308205212 1107 68-69 0.355615522584813 1107 70-71 0.3980925694101245 1107 72-73 0.26553738132520266 1107 74-75 0.37920176750428425 1107 76-77 0.27607862446149767 1107 78-79 0.3538346119456719 1107 80-81 0.4141848618544728 1107 82-83 0.4340781876831805 1107 84-85 0.5264476092794546 1107 86-87 0.6890045732294219 1107 88-89 0.8005528683880954 1107 90-91 0.793447024497965 1107 92-93 0.8831572476113685 1107 94-95 0.7418858118186193 1107 96-97 0.9000133682478193 1107 98-99 0.9977433737917458 1107 100-101 0.9870599023819508 1108 1 0.0111669495270732 1108 2 0.023412861663423712 1108 3 0.0030008230287350557 1108 4 0.010334417052845879 1108 5 -0.0928549572618067 1108 6 -0.006257253976045263 1108 7 0.02182633574858528 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985.0 10 1822.0 11 2874.0 12 4586.0 13 6556.0 14 8730.0 15 12510.0 16 16169.0 17 23324.0 18 22753.0 19 16270.0 20 13777.0 21 13286.0 22 13671.0 23 14471.0 24 16174.0 25 19219.0 26 22445.0 27 27467.0 28 33104.0 29 41039.0 30 50269.0 31 62496.0 32 78351.0 33 102299.0 34 144494.0 35 220691.0 36 372034.0 37 612092.0 38 544946.0 39 63947.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.321907867083745 18.716748293727374 14.889720254115993 23.071623585072892 2 15.858123950699676 19.897501963597286 39.81327534995388 24.43109873574916 3 19.450880543506955 23.721917168759806 29.721681830743968 27.105520456989268 4 12.94541707983351 16.833791203474703 36.46465420547726 33.756137511214526 5 13.656533786223715 37.408701703486585 34.062687594268844 14.872076916020854 6 32.63309777985232 36.06751088167289 16.816683967423486 14.482707371051307 7 28.988288897094172 32.94803348064134 19.81668164517425 18.24699597709024 8 26.847570722493796 34.51172547790176 19.232144723244662 19.40855907635978 9 26.34912611453924 16.79206220867743 19.927808704678693 36.931002972104636 10-11 25.163795979567304 26.561557795751987 26.634070028199847 21.64057619648086 12-13 26.211749652243178 23.847583815780613 27.24793726211459 22.692729269861616 14-15 22.721544512486346 25.220768494199408 25.904283853168824 26.15340314014542 16-17 22.074677201455977 28.47802961148449 26.841032034178973 22.606261152880556 18-19 22.058116609423223 26.61603389245354 29.453532179794728 21.87231731832851 20-21 22.46932889319279 25.77779867802092 29.95968575323315 21.79318667555314 22-23 22.270486302212873 25.78530728388955 29.90704810384479 22.037158310052785 24-25 22.057872947209194 26.02908549301815 29.925575447174356 21.987466112598295 26-27 21.568201614308112 26.402609217428335 29.72473530379575 22.304453864467803 28-29 21.840468026201943 26.81668141617753 29.522450639131492 21.820399918489038 30-31 21.802843361966666 26.24499652049656 29.490881688368088 22.461278429168686 32-33 21.43578701942299 26.310363739317122 29.58966455353988 22.66418468772001 34-35 22.389147095963995 26.432081474203684 29.25308087107531 21.92569055875701 36-37 22.492822036830752 25.93089028418464 29.274865091449843 22.301422587534766 38-39 21.887864906301818 26.348199113933436 29.240932179654454 22.52300380011029 40-41 22.380868601507327 26.22804007333992 29.292031338821246 22.099059986331508 42-43 22.143435541407584 26.217752898407117 29.047396388138825 22.59141517204647 44-45 22.029998577578574 26.560672079609155 29.26782066652539 22.141508676286882 46-47 22.59480152595086 26.885294948079313 28.36203344776223 22.157870078207594 48-49 22.256591510940503 26.890562456506206 28.34140701984981 22.511439012703477 50-51 22.47602471182822 27.11173734432222 28.278648234200382 22.13358970964918 52-53 22.808241817361292 26.78613864911665 28.214070353314742 22.191549180207314 54-55 22.232498175099142 27.025214208140103 28.44018002076091 22.30210759599985 56-57 22.37674855691562 27.12191653681423 28.16083278953997 22.34050211673018 58-59 22.30063683814929 26.704434180102815 28.168728436948225 22.826200544799672 60-61 21.92272947886405 27.065137542951934 28.471820666624865 22.54031231155915 62-63 22.08091335610686 27.009210040476805 28.628146744477494 22.28172985893884 64-65 21.765222924315573 27.069878801812287 28.398514999020787 22.766383274851357 66-67 21.734008024145197 27.088456795714055 28.37461518394923 22.802919996191513 68-69 22.338810246104995 26.709006561922582 28.477211310627343 22.47497188134508 70-71 22.062451474666968 26.866101431202203 28.08141186560989 22.990035228520934 72-73 22.076133393092547 27.02442077298388 28.031231929998118 22.86821390392545 74-75 22.083583942730236 27.370919711328938 27.871944984367392 22.673551361573434 76-77 22.174345183771194 26.9484638708334 28.117909882796084 22.759281062599324 78-79 22.24445775867341 27.09234656318724 28.019311050577816 22.643884627561537 80-81 22.42238075443685 27.214380760629513 27.73735128896444 22.625887195969195 82-83 22.310762292006256 27.35118588583085 27.795258082251372 22.542793739911517 84-85 22.232436118850018 27.436382046008205 27.72264638922197 22.608535445919802 86-87 22.35823546475793 27.416050930169956 27.4529167438333 22.77279686123882 88-89 22.138788919226513 27.55721880303077 27.318472090708656 22.985520187034062 90-91 22.117591688955486 27.47910017999419 27.44726592095157 22.956042210098758 92-93 22.191817882563946 27.14849718165318 27.695426957240123 22.96425797854275 94-95 21.93244422150852 26.947147929598692 27.87372537544964 23.246682473443144 96-97 22.240765816020154 26.609730264893216 27.832859175102076 23.31664474398455 98-99 21.83727070691589 26.875487188538 27.533786791201152 23.753455313344958 100-101 21.953344464648016 26.615414625990148 27.89172280704199 23.53951810231985 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1055.0 1 690.0 2 492.0 3 716.5 4 594.0 5 506.5 6 1073.0 7 2477.0 8 4067.0 9 4659.0 10 3809.0 11 2893.0 12 2781.5 13 2865.5 14 3051.0 15 3228.0 16 3406.5 17 3623.5 18 3885.5 19 4268.5 20 4819.5 21 5628.5 22 6641.5 23 8105.0 24 10100.0 25 12765.5 26 16320.0 27 20117.0 28 23297.5 29 26731.0 30 30808.5 31 36456.5 32 45427.5 33 49363.0 34 49325.0 35 52589.0 36 57691.0 37 62555.0 38 65408.5 39 68620.0 40 72345.5 41 76393.5 42 78398.5 43 80260.0 44 83535.5 45 88445.0 46 91612.0 47 94355.0 48 94984.5 49 92091.5 50 91590.5 51 89007.0 52 87055.5 53 89132.5 54 92179.5 55 88032.5 56 79919.0 57 73444.5 58 68219.0 59 60059.0 60 47395.0 61 38836.0 62 33037.0 63 24423.0 64 16747.0 65 12720.0 66 9497.5 67 7245.0 68 5560.5 69 4158.0 70 3148.5 71 2115.5 72 1499.5 73 1228.5 74 916.0 75 631.0 76 439.5 77 258.0 78 150.5 79 101.0 80 62.5 81 40.5 82 23.0 83 10.5 84 7.0 85 4.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01675889866555818 3 0.0071215643290131764 4 0.0 5 3.870415396202813E-5 6 0.0 7 0.0 8 4.644498475443376E-4 9 4.2574569358230937E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.289853086772391E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005650806478456107 26-27 0.021364692987039528 28-29 1.9352076981014066E-5 30-31 0.0 32-33 4.2574569358230937E-4 34-35 0.019506893596862175 36-37 0.003870415396202813 38-39 0.018868275056488713 40-41 0.015036563814247927 42-43 0.00828268894787402 44-45 0.0030769802399812363 46-47 5.80562309430422E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.0963323169622505E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.9352076981014066E-5 84-85 0.0029415157011141377 86-87 2.90281154715211E-4 88-89 5.80562309430422E-5 90-91 2.90281154715211E-4 92-93 2.5157700075318284E-4 94-95 0.0 96-97 1.9352076981014066E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2583702.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.78393967708846 #Duplication Level Percentage of deduplicated Percentage of total 1 73.8704394361148 37.514319402436705 2 14.642883560866684 14.872466309073678 3 4.958150412323337 7.55383234348079 4 2.213662730013612 4.4967405818572095 5 1.1459572230463135 2.9098111243853895 6 0.7137082526923051 2.1746950111059737 7 0.44094765961359894 1.5675141542599242 8 0.32353021453978187 1.3144111119123014 9 0.22575438057168834 1.0318227160311981 >10 1.3012108112410898 12.267645069988697 >50 0.10368606126219006 3.6170920902963135 >100 0.05250425748839165 4.889661660885255 >500 0.003667878897518702 1.2261567411013634 >1k 0.0036678788975068725 3.430693194086 >5k 7.641414369805984E-5 0.25282182385211743 >10k+ 1.528282873961197E-4 0.8803166652471656 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12477 0.482911728984225 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10208 0.3950920036443832 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6515 0.2521575630626133 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4139 0.16019649324883445 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3787 0.14657263105420051 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3599 0.13929625010933924 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3432 0.13283265639768055 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3428 0.13267783978183242 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2885 0.11166148418045115 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2856 0.11053906371555233 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2786 0.10782977293821037 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2723 0.1053914112386026 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.870415396202813E-5 0.0 3 0.0 0.0 0.0 3.870415396202813E-5 0.0 4 0.0 0.0 0.0 3.870415396202813E-5 0.0 5 0.0 0.0 0.0 3.870415396202813E-5 0.0 6 0.0 0.0 0.0 7.740830792405626E-5 3.870415396202813E-5 7 0.0 0.0 0.0 1.1611246188608438E-4 3.870415396202813E-5 8 3.870415396202813E-5 0.0 0.0 1.1611246188608438E-4 3.870415396202813E-5 9 3.870415396202813E-5 0.0 0.0 1.1611246188608438E-4 3.870415396202813E-5 10-11 3.870415396202813E-5 0.0 0.0 1.1611246188608438E-4 3.870415396202813E-5 12-13 3.870415396202813E-5 0.0 0.0 1.9352076981014063E-4 1.548166158481125E-4 14-15 3.870415396202813E-5 0.0 0.0 1.9352076981014063E-4 2.5157700075318284E-4 16-17 3.870415396202813E-5 0.0 0.0 2.1287284679115468E-4 2.709290777341969E-4 18-19 3.870415396202813E-5 0.0 0.0 2.5157700075318284E-4 2.709290777341969E-4 20-21 3.870415396202813E-5 0.0 0.0 3.4833738565825316E-4 3.289853086772391E-4 22-23 3.870415396202813E-5 0.0 0.0 5.031540015063657E-4 3.8704153962028126E-4 24-25 3.870415396202813E-5 0.0 0.0 6.966747713165063E-4 3.8704153962028126E-4 26-27 3.870415396202813E-5 0.0 0.0 0.0012965891577279422 4.257456935823094E-4 28-29 3.870415396202813E-5 0.0 0.0 0.0031737406248863063 4.257456935823094E-4 30-31 3.870415396202813E-5 0.0 0.0 0.010585586108614693 4.450977705633235E-4 32-33 3.870415396202813E-5 0.0 0.0 0.029357100780198334 4.644498475443375E-4 34-35 3.870415396202813E-5 0.0 0.0 0.056043614937016734 5.031540015063657E-4 36-37 3.870415396202813E-5 0.0 0.0 0.1009984897639124 5.031540015063657E-4 38-39 3.870415396202813E-5 0.0 0.0 0.1698144755083984 5.031540015063657E-4 40-41 3.870415396202813E-5 0.0 0.0 0.23911426317740977 5.031540015063657E-4 42-43 3.870415396202813E-5 0.0 0.0 0.3114716790094214 5.418581554683938E-4 44-45 3.870415396202813E-5 0.0 0.0 0.39669822603380733 5.805623094304219E-4 46-47 3.870415396202813E-5 0.0 0.0 0.49610984548527653 6.192664633924501E-4 48-49 3.870415396202813E-5 0.0 0.0 0.6010368068763348 6.579706173544782E-4 50-51 3.870415396202813E-5 0.0 0.0 0.7160074962205394 6.579706173544782E-4 52-53 3.870415396202813E-5 0.0 0.0 0.824185606544408 6.966747713165063E-4 54-55 3.870415396202813E-5 0.0 0.0 0.9394465770433278 6.966747713165063E-4 56-57 3.870415396202813E-5 0.0 0.0 1.0672863975799065 6.966747713165063E-4 58-59 3.870415396202813E-5 0.0 0.0 1.2058085646100052 6.966747713165063E-4 60-61 3.870415396202813E-5 0.0 0.0 1.3604123076113268 6.966747713165063E-4 62-63 3.870415396202813E-5 0.0 0.0 1.5048175060436537 6.966747713165063E-4 64-65 3.870415396202813E-5 0.0 0.0 1.6542155403370824 6.966747713165063E-4 66-67 3.870415396202813E-5 0.0 0.0 1.8306484261729876 6.966747713165063E-4 68-69 3.870415396202813E-5 0.0 0.0 2.0234144649808687 6.966747713165063E-4 70-71 3.870415396202813E-5 0.0 0.0 2.2229537307321046 6.966747713165063E-4 72-73 3.870415396202813E-5 0.0 0.0 2.4276987051912333 7.547310022595485E-4 74-75 3.870415396202813E-5 0.0 0.0 2.6245673843190893 7.740830792405625E-4 76-77 3.870415396202813E-5 0.0 0.0 2.8398398886558898 7.740830792405625E-4 78-79 3.870415396202813E-5 0.0 0.0 3.089036583940408 7.740830792405625E-4 80-81 3.870415396202813E-5 0.0 0.0 3.350657312646737 8.127872332025907E-4 82-83 3.870415396202813E-5 0.0 0.0 3.616380681673041 8.127872332025907E-4 84-85 3.870415396202813E-5 0.0 0.0 3.8825878526238706 8.127872332025907E-4 86-87 3.870415396202813E-5 0.0 0.0 4.167585890323265 8.708434641456329E-4 88-89 3.870415396202813E-5 0.0 0.0 4.465375650907109 9.28899695088675E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5495 0.0 57.491756 1 GTATCAA 10080 0.0 41.8037 1 ATCAACG 12610 0.0 33.14759 3 TCAACGC 12860 0.0 32.501312 4 TATCAAC 12965 0.0 32.2816 2 CAACGCA 13040 0.0 32.01625 5 AACGCAG 13485 0.0 30.99495 6 TGGTATC 2310 0.0 29.408693 2 GTGGTAT 2575 0.0 28.411564 1 ACGCAGA 15395 0.0 27.087818 7 CGCAGAG 15580 0.0 26.705202 8 TAAGGTG 1570 0.0 24.504429 5 GCAGAGT 17355 0.0 23.672857 9 TACATGG 9720 0.0 23.1178 2 GTACATG 10325 0.0 23.051508 1 GAGTACT 10805 0.0 22.814018 12-13 GTAAGGT 1520 0.0 22.810692 4 GGACCGA 695 0.0 22.55217 6 CAGAGTA 16500 0.0 21.689949 10-11 GTACTTT 11265 0.0 21.671608 14-15 >>END_MODULE