FastQCFastQC Report
Thu 26 May 2016
SRR938260_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938260_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556403
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120270.4704657286038234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96270.3765838171837539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59440.23251420061703884No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40670.15909072239392616No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36840.14410873402980673No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35310.1381237621767773No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33800.13221702524993126No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC31210.12208560230918207No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28530.11160212220060765No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27490.10753390603907131No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA27140.10616479483086196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56600.055.2835461
GTATCAA102100.043.0549351
TATCAAC128550.033.888182
ATCAACG129000.033.6968883
TCAACGC131250.033.119234
CAACGCA132300.032.748535
AACGCAG137350.031.5722716
TGGTATC24750.031.128962
GTGGTAT26550.030.495191
ACGCAGA153300.027.93857
CGCAGAG153900.027.6745998
GCAGAGT168550.024.9020679
GTACATG98350.024.890621
TACATGG95750.024.5861912
TAAGGTG16250.023.9990355
GTAAGGT16200.023.1923854
GAGTACT107900.023.1710912-13
ACATGGG98350.023.1146243
CAGAGTA159700.022.57548510-11
AGGACCG8200.022.0395665