FastQCFastQC Report
Thu 26 May 2016
SRR938260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556403
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118990.46545869332808637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98200.3841334875604512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62000.24252827116851297No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39290.15369251248727214No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39110.15298839815162163No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35380.13839758441841918No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35280.1380064097875022No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33030.1292049805918707No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA29370.11488798910031009No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG29300.11461416685866821No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA27040.105773620199945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57850.056.5774151
GTATCAA100850.041.0269741
ATCAACG129150.031.625533
TATCAAC130250.031.4715272
TCAACGC132800.030.790264
CAACGCA134750.030.3799325
AACGCAG139150.029.4193026
TGGTATC24850.026.951162
ACGCAGA156750.025.8742127
CGCAGAG158200.025.5469998
GTATAGG6400.025.236331
GTGGTAT26200.025.2023331
TACATGG94500.023.3222772
GTACATG98850.023.2112051
GAGTACT106450.023.20018612-13
TAAGGTG17050.023.1186265
GCAGAGT175500.022.8392669
GTACTTT112050.022.03983314-15
GGACCGA7350.021.9684736
GTAAGGT16800.021.7665584
Produced by FastQC (version 0.11.5)