##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938260_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2556403 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.532335472928175 34.0 31.0 34.0 31.0 34.0 2 32.746211375905915 34.0 31.0 34.0 31.0 34.0 3 32.7985720561273 34.0 31.0 34.0 31.0 34.0 4 36.24996919499782 37.0 35.0 37.0 35.0 37.0 5 36.22447086785613 37.0 37.0 37.0 35.0 37.0 6 36.256752163097914 37.0 37.0 37.0 35.0 37.0 7 36.217124999462136 37.0 37.0 37.0 35.0 37.0 8 36.20325785879613 37.0 37.0 37.0 35.0 37.0 9 37.89699472266305 39.0 38.0 39.0 35.0 39.0 10-11 37.876491499970854 39.0 38.0 39.0 35.0 39.0 12-13 37.7960429165511 39.0 38.0 39.0 35.0 39.0 14-15 39.08063536930601 40.0 38.5 41.0 36.0 41.0 16-17 38.96981090227167 40.0 38.0 41.0 36.0 41.0 18-19 39.22043453242701 40.5 39.0 41.0 36.0 41.0 20-21 39.20787547972679 41.0 39.0 41.0 36.0 41.0 22-23 39.11469435765801 40.0 39.0 41.0 35.5 41.0 24-25 39.011929848306394 40.0 38.5 41.0 35.0 41.0 26-27 38.83991823667864 40.0 38.0 41.0 35.0 41.0 28-29 38.726167783405046 40.0 38.0 41.0 35.0 41.0 30-31 38.475315120503296 40.0 38.0 41.0 34.0 41.0 32-33 38.25719673306595 40.0 38.0 41.0 34.0 41.0 34-35 38.10283824576955 40.0 38.0 41.0 34.0 41.0 36-37 38.0887833412807 40.0 38.0 41.0 34.0 41.0 38-39 37.93378078495448 40.0 37.5 41.0 33.0 41.0 40-41 37.75203889997 40.0 37.0 41.0 33.0 41.0 42-43 37.50928042253119 40.0 37.0 41.0 33.0 41.0 44-45 37.229572762979856 40.0 37.0 41.0 32.0 41.0 46-47 36.82959807197848 40.0 36.0 41.0 31.0 41.0 48-49 36.4174302721441 39.0 36.0 41.0 30.0 41.0 50-51 36.02695682175306 39.0 35.0 41.0 29.5 41.0 52-53 35.51460313573408 38.5 35.0 40.0 28.0 41.0 54-55 35.198746441777764 38.0 34.5 40.0 27.0 41.0 56-57 34.85255826252747 38.0 34.0 40.0 26.0 41.0 58-59 34.41037563326283 38.0 34.0 40.0 25.5 41.0 60-61 33.9365100103544 37.0 33.0 40.0 24.0 41.0 62-63 33.47376626455218 36.5 33.0 39.0 22.5 41.0 64-65 32.98495992220319 36.0 32.0 39.0 22.0 40.0 66-67 32.44190939378494 35.0 31.5 39.0 20.0 40.0 68-69 31.862137151302043 35.0 31.0 38.0 18.0 40.0 70-71 31.246755695404833 35.0 30.5 37.5 13.5 39.5 72-73 30.66702374390892 34.5 30.0 37.0 8.0 39.0 74-75 30.08049259056573 34.0 29.0 36.0 7.0 39.0 76-77 28.727357541044974 32.5 28.0 35.0 6.0 37.0 78-79 29.409881775291296 34.0 29.0 35.0 2.0 37.0 80-81 29.098407215137833 34.0 29.0 35.0 2.0 37.0 82-83 28.544452107120826 34.0 28.5 35.0 2.0 36.5 84-85 27.956087518282523 33.0 26.5 35.0 2.0 36.0 86-87 27.32888867678531 33.0 26.0 35.0 2.0 35.5 88-89 26.583107788560724 32.0 25.0 35.0 2.0 35.0 90-91 26.04439714708518 32.0 24.0 34.0 2.0 35.0 92-93 25.234580776192175 31.5 20.5 34.0 2.0 35.0 94-95 24.417172292475016 31.0 15.5 34.0 2.0 35.0 96-97 23.373363667622044 31.0 2.0 34.0 2.0 35.0 98-99 21.93175606506486 30.0 2.0 34.0 2.0 35.0 100-101 17.544743336633545 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14299498783067577 1101 2 0.025211058338079795 1101 3 -0.0039609108089067036 1101 4 0.009019885379004222 1101 5 0.06973706609136343 1101 6 0.10622506567034407 1101 7 0.0343621031260426 1101 8 0.037161674149615465 1101 9 -0.024106005228027527 1101 10-11 0.06891643973002459 1101 12-13 0.1816092792078834 1101 14-15 0.12681787638648245 1101 16-17 0.17050415603178948 1101 18-19 0.18895811982676491 1101 20-21 0.15267918116042978 1101 22-23 0.20276609298353776 1101 24-25 0.238360163029661 1101 26-27 0.06480136190124597 1101 28-29 0.2055497329974756 1101 30-31 0.1768373823419367 1101 32-33 0.27397601686506334 1101 34-35 0.26984242444063966 1101 36-37 0.33298670496790805 1101 38-39 0.3426905960129929 1101 40-41 0.27914760212134837 1101 42-43 0.19954384626519328 1101 44-45 0.2736919545732448 1101 46-47 0.4356063391637264 1101 48-49 0.4640680476283592 1101 50-51 0.24193294665117548 1101 52-53 0.07766827402969056 1101 54-55 -0.010730539943835993 1101 56-57 0.01517120645456771 1101 58-59 0.2748902224781915 1101 60-61 0.24723698981326692 1101 62-63 0.06865618417809571 1101 64-65 0.08806346132623588 1101 66-67 -0.042673974181361984 1101 68-69 0.22498288589323678 1101 70-71 0.19161078966159906 1101 72-73 0.07779178880352688 1101 74-75 -0.007643579753281671 1101 76-77 0.005927099951115622 1101 78-79 0.020894982433091513 1101 80-81 -0.1223247128537075 1101 82-83 -0.002198499561743006 1101 84-85 -0.03964489682183725 1101 86-87 -0.17169195008980864 1101 88-89 -0.25492848960067604 1101 90-91 -0.34384672714577746 1101 92-93 -0.4084074024104787 1101 94-95 -0.3429496106551895 1101 96-97 -0.492020942547704 1101 98-99 -0.6557162262593366 1101 100-101 -1.2074648729094548 1102 1 0.14856435840555093 1102 2 0.1818097706340538 1102 3 0.1685888841722729 1102 4 0.0944162540113922 1102 5 0.11357683122477624 1102 6 0.053686042882496565 1102 7 0.13372868694571594 1102 8 0.18822163339381603 1102 9 0.12231906474487175 1102 10-11 0.18953287827817888 1102 12-13 0.2222446618094125 1102 14-15 0.2323038681150038 1102 16-17 0.21743208647912837 1102 18-19 0.2488452403500503 1102 20-21 0.25229210667122715 1102 22-23 0.24040807366129968 1102 24-25 0.3250765350170397 1102 26-27 0.22498264782427668 1102 28-29 0.09209445280077233 1102 30-31 0.27930064832174395 1102 32-33 0.16307827012071385 1102 34-35 0.13265856384265362 1102 36-37 0.19926726023178531 1102 38-39 0.1344703085403225 1102 40-41 0.050092952645634625 1102 42-43 0.16328439768678749 1102 44-45 0.047664127557069946 1102 46-47 0.1340727893659448 1102 48-49 0.03373617308344734 1102 50-51 0.08632229632419808 1102 52-53 -0.020088518047359116 1102 54-55 0.04874744853664481 1102 56-57 0.15001067037192684 1102 58-59 0.21326620436814636 1102 60-61 0.21968046436741417 1102 62-63 0.042840006180753676 1102 64-65 0.07433624577650733 1102 66-67 -0.0680352016594199 1102 68-69 -0.009949142127400279 1102 70-71 8.641597992067318E-4 1102 72-73 0.02300043752070735 1102 74-75 -0.18609457888715397 1102 76-77 -0.1708580471865666 1102 78-79 -0.3424978229233382 1102 80-81 -0.5161225513859478 1102 82-83 -0.5388146854075231 1102 84-85 -0.6199724482587392 1102 86-87 -0.6698253592626031 1102 88-89 -0.7397791763495647 1102 90-91 -0.851404511791177 1102 92-93 -0.7425837597747567 1102 94-95 -0.6842126309185979 1102 96-97 -1.0695197151515785 1102 98-99 -1.3041991387706275 1102 100-101 -1.4188718623725016 1103 1 0.2080282460726508 1103 2 0.17704826519121042 1103 3 0.1959974246048546 1103 4 0.088749455780615 1103 5 0.14475430046074678 1103 6 0.12197809747179633 1103 7 0.12568768732186442 1103 8 0.14087649369328403 1103 9 0.20776874590683292 1103 10-11 0.1708288358465424 1103 12-13 0.20358315261719184 1103 14-15 0.2611807124147205 1103 16-17 0.191397813154488 1103 18-19 0.2787143589202259 1103 20-21 0.30258232043804156 1103 22-23 0.2785242059344313 1103 24-25 0.24728364326877994 1103 26-27 0.3445894431442511 1103 28-29 0.37961350708730635 1103 30-31 0.4190173003470292 1103 32-33 0.38839387280462034 1103 34-35 0.37983242158327357 1103 36-37 0.3267870522986627 1103 38-39 0.2677445858481704 1103 40-41 0.3875824150859515 1103 42-43 0.20823225516051735 1103 44-45 0.2919231481314668 1103 46-47 0.3472101781798287 1103 48-49 0.34471694523711705 1103 50-51 0.37200607156646726 1103 52-53 0.3284075791751562 1103 54-55 0.3579789152319037 1103 56-57 0.497688758658029 1103 58-59 0.4431548358178219 1103 60-61 0.3942845524769325 1103 62-63 0.3056349520224373 1103 64-65 0.2638862320086304 1103 66-67 0.27769784763518857 1103 68-69 0.30667736321666794 1103 70-71 0.3149611468735394 1103 72-73 0.1730996347108551 1103 74-75 0.1633847215358024 1103 76-77 0.28744627552098123 1103 78-79 0.10605741767571075 1103 80-81 0.08345753870388606 1103 82-83 0.027574057112374817 1103 84-85 -0.1669545435791555 1103 86-87 -0.2831724107415283 1103 88-89 -0.5451878925593228 1103 90-91 -0.5401557399132635 1103 92-93 -0.5079231407568763 1103 94-95 -0.6141119057571025 1103 96-97 -0.6731337100790356 1103 98-99 -0.6133804122117859 1103 100-101 -1.2720017645299215 1104 1 0.13412013416790103 1104 2 0.12642434537299607 1104 3 0.08563495329289594 1104 4 0.07115703086201108 1104 5 0.04517186341152524 1104 6 0.06020476952311071 1104 7 0.0771784802520088 1104 8 0.10072460715888099 1104 9 0.160493058132694 1104 10-11 0.08769471183844502 1104 12-13 0.09581311758918076 1104 14-15 0.14480616049364414 1104 16-17 0.05923508559177293 1104 18-19 0.09352943331401775 1104 20-21 0.1099839665953084 1104 22-23 0.08614520334236886 1104 24-25 0.16751896662434262 1104 26-27 0.14854890369929308 1104 28-29 0.08409925910597593 1104 30-31 0.040850440343142225 1104 32-33 0.003919054252428111 1104 34-35 0.04567598436281628 1104 36-37 0.12361759720656806 1104 38-39 0.09318588343773371 1104 40-41 0.0881927722534428 1104 42-43 0.01840723608567174 1104 44-45 0.046960839419774913 1104 46-47 0.07752842312735453 1104 48-49 0.04925971342716906 1104 50-51 0.16247978692203446 1104 52-53 0.09639741249093703 1104 54-55 0.004328061807790107 1104 56-57 0.1808241328545961 1104 58-59 0.167766917487711 1104 60-61 0.04747639730808828 1104 62-63 0.09944667060727852 1104 64-65 0.09587662298424249 1104 66-67 5.346616700308005E-4 1104 68-69 0.1853516860880724 1104 70-71 0.02308835696051048 1104 72-73 -0.2991378465457899 1104 74-75 -0.1851303197469356 1104 76-77 -0.11867309399002579 1104 78-79 -0.17583148072639787 1104 80-81 -0.36832837447544975 1104 82-83 -0.456155308147995 1104 84-85 -0.43012660512479783 1104 86-87 -0.704268469920148 1104 88-89 -0.8291782441350506 1104 90-91 -0.9309227949465608 1104 92-93 -0.7325445041290806 1104 94-95 -0.859803869924427 1104 96-97 -1.3143900434201967 1104 98-99 -1.2981866822479482 1104 100-101 -1.4547950416982225 1105 1 0.05958328349948516 1105 2 0.017743947065973487 1105 3 0.06387315476110444 1105 4 0.04715985714222626 1105 5 0.00414712018090313 1105 6 0.07800879732185706 1105 7 0.031944019202121865 1105 8 0.0446434920781158 1105 9 0.06085899292976649 1105 10-11 0.07820301595850054 1105 12-13 0.10465747369489975 1105 14-15 0.08746286389356328 1105 16-17 0.054146348500133 1105 18-19 -4.311049931189359E-4 1105 20-21 0.10657516794611155 1105 22-23 0.12810243034495272 1105 24-25 0.03924103369468668 1105 26-27 0.15088474322472223 1105 28-29 0.03487627649976588 1105 30-31 -0.03895493490076518 1105 32-33 0.0729506339501711 1105 34-35 0.0918474719199196 1105 36-37 0.04892279912765929 1105 38-39 0.13550670398151254 1105 40-41 0.04572239088732033 1105 42-43 -0.04549052860902947 1105 44-45 0.031061440738149315 1105 46-47 0.08708441618326646 1105 48-49 0.18722607761754517 1105 50-51 0.05636184074157313 1105 52-53 -0.07533300622345251 1105 54-55 0.0029248807749056027 1105 56-57 0.0024752983107987347 1105 58-59 -0.0916583845692358 1105 60-61 -0.16185371697315531 1105 62-63 -0.17528293181253218 1105 64-65 -0.10620276166383036 1105 66-67 -0.22652207918998357 1105 68-69 -0.15405738093195254 1105 70-71 -0.3289100272234968 1105 72-73 -0.25014042821718263 1105 74-75 -0.1815261834816546 1105 76-77 -0.24238086052977437 1105 78-79 -0.4175623047925505 1105 80-81 -0.26581910729552405 1105 82-83 -0.26640193673954826 1105 84-85 -0.29185902264270425 1105 86-87 -0.30318513239352285 1105 88-89 -0.5488091853338872 1105 90-91 -0.8584846879672448 1105 92-93 -0.7316328121069624 1105 94-95 -0.8291409310962017 1105 96-97 -1.4707944964189608 1105 98-99 -1.4765577063062736 1105 100-101 -1.93803234859703 1106 1 0.20188218419183812 1106 2 0.2606706775065746 1106 3 0.19842823465469905 1106 4 0.08976133169431222 1106 5 0.1085141814708237 1106 6 0.12448053066928111 1106 7 0.15056001906118865 1106 8 0.20716132940172116 1106 9 0.18926570152515865 1106 10-11 0.22772328003897968 1106 12-13 0.167323735168047 1106 14-15 0.13936286917869722 1106 16-17 0.2616577335572927 1106 18-19 0.2787075945118431 1106 20-21 0.32226694780271004 1106 22-23 0.3953314131330359 1106 24-25 0.4201928641123871 1106 26-27 0.2879574182066591 1106 28-29 0.3350784328339884 1106 30-31 0.351255237218389 1106 32-33 0.3689692772565536 1106 34-35 0.4092587917937749 1106 36-37 0.3868068521199248 1106 38-39 0.468304917606595 1106 40-41 0.3153852874924965 1106 42-43 0.3142629639829764 1106 44-45 0.1799684091859035 1106 46-47 0.22533523537886424 1106 48-49 0.2034822656273505 1106 50-51 0.025801660694710904 1106 52-53 -0.05017467244969254 1106 54-55 -0.04802545218847598 1106 56-57 -0.17103011014189207 1106 58-59 -0.1579939596797857 1106 60-61 -0.2040824795473668 1106 62-63 -0.14862053729497404 1106 64-65 -0.20488169864742645 1106 66-67 -0.4294436566259918 1106 68-69 -0.4664296568863584 1106 70-71 -0.8089564483076579 1106 72-73 -0.6870816355177105 1106 74-75 -0.6030882045164816 1106 76-77 -0.6273323253924517 1106 78-79 -0.5545793757718904 1106 80-81 -0.4868433660556306 1106 82-83 -0.524499751337089 1106 84-85 -0.7988420045146967 1106 86-87 -0.833168352096493 1106 88-89 -0.8110514249970464 1106 90-91 -1.039596283548871 1106 92-93 -0.8152160881846875 1106 94-95 -1.0777816609730557 1106 96-97 -1.2686849576348962 1106 98-99 -1.1812910933717937 1106 100-101 -1.8135344362245327 1107 1 -0.06171853180027398 1107 2 -0.007829848343668289 1107 3 0.040666197559616535 1107 4 0.016180604976241852 1107 5 -0.04563655297828717 1107 6 -0.006543240547557616 1107 7 -0.06375007238800379 1107 8 -0.03253931903082474 1107 9 0.017023629326963885 1107 10-11 -0.010883878489202914 1107 12-13 -0.04432576301063307 1107 14-15 0.0010189840160350627 1107 16-17 -0.09678233692753224 1107 18-19 0.013161929119746674 1107 20-21 -0.035208483498969656 1107 22-23 -0.010308069513911278 1107 24-25 0.04963734338134884 1107 26-27 0.05489954682057885 1107 28-29 0.06436132633364622 1107 30-31 0.02752824751948424 1107 32-33 -0.055567730760046175 1107 34-35 0.031515490290054515 1107 36-37 -0.02493910997706905 1107 38-39 -0.036042923621458556 1107 40-41 -0.08698069034517886 1107 42-43 -0.05372424845355539 1107 44-45 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-0.5917969259094846 2316 46-47 -0.592491540412972 2316 48-49 -0.36159225635034176 2316 50-51 -0.3071338204678966 2316 52-53 -0.23088201789931873 2316 54-55 -0.235052227544152 2316 56-57 -0.2395609984085354 2316 58-59 -0.18406193174862295 2316 60-61 0.036850198249894106 2316 62-63 -0.029076795636377994 2316 64-65 0.07219133060090854 2316 66-67 0.13176029120153743 2316 68-69 0.2506795947644811 2316 70-71 0.4073696383644183 2316 72-73 0.5238706527524215 2316 74-75 0.6945981740283393 2316 76-77 0.6271331869986199 2316 78-79 0.7401541191556653 2316 80-81 0.7492496629310139 2316 82-83 0.9559352991256702 2316 84-85 1.0957557927009098 2316 86-87 1.3157260850060588 2316 88-89 1.5484586457467628 2316 90-91 1.6225350864473747 2316 92-93 1.655915572942849 2316 94-95 2.1678280628120383 2316 96-97 2.463486113074026 2316 98-99 3.073641782874514 2316 100-101 3.2859557296439768 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 12.0 5 36.0 6 79.0 7 232.0 8 572.0 9 1065.0 10 2036.0 11 3264.0 12 5201.0 13 7767.0 14 10317.0 15 14713.0 16 19661.0 17 27229.0 18 27891.0 19 21866.0 20 20189.0 21 20598.0 22 22505.0 23 23898.0 24 26902.0 25 30846.0 26 35447.0 27 41966.0 28 50541.0 29 60312.0 30 73590.0 31 89659.0 32 111597.0 33 144288.0 34 195720.0 35 281026.0 36 400889.0 37 498123.0 38 272444.0 39 13911.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.30784469902652 18.747721201859655 14.874703267229277 23.069730831884552 2 15.809712718551816 19.948044428273533 39.785057295884634 24.457185557290014 3 19.445290974408366 23.707498708036617 29.676662935849375 27.17054738170564 4 12.971013925833203 16.79580660303552 36.39133156000626 33.841847911125015 5 13.63360938764605 37.44852586527595 34.03717990393451 14.880684843143491 6 32.59234600393371 36.05855817090211 16.83293146046494 14.516164364699236 7 28.978334010717404 32.900602917458635 19.827390282361584 18.29367278946238 8 26.876709188652963 34.52100470856903 19.190049456208584 19.41223664656942 9 26.36348807288992 16.776736688229516 19.89717583651717 36.9625994023634 10-11 25.18151480811124 26.546636035085236 26.614680862133238 21.657168294670285 12-13 26.223594653593974 23.8456538887247 27.233013668005608 22.697737789675717 14-15 22.705535864259275 25.256190045153286 25.874265520733626 26.164008569853813 16-17 22.086730858864854 28.51490230050187 26.78987307407269 22.608493766560585 18-19 22.076585733939446 26.62719845032258 29.436145240011065 21.86007057572691 20-21 22.44558076328341 25.77842773615897 29.980132240495728 21.795859260061892 22-23 22.284416815345626 25.759866499921962 29.885898271907834 22.06981841282458 24-25 22.084972322588683 26.046770629651572 29.874274667183553 21.993982380576192 26-27 21.57774314797433 26.407192235446843 29.728908108734103 22.28615650784472 28-29 21.843300954124224 26.81243798212086 29.516999427083405 21.827261636671512 30-31 21.827172780081558 26.274354248486436 29.45930345934923 22.439169512082774 32-33 21.49580875941704 26.29309619805641 29.569692259006114 22.64140278352044 34-35 22.404241501934536 26.444680909329193 29.255350778704077 21.895726810032194 36-37 22.50388977392859 25.931076433201994 29.245838602008895 22.319195190860523 38-39 21.90359189169207 26.394522483502787 29.196887269734358 22.504998355070782 40-41 22.443852716971847 26.183236535429128 29.263655271935807 22.10925547566322 42-43 22.11634790929038 26.229309718256584 29.021316862865675 22.633025509587366 44-45 22.080188859148745 26.534240989046133 29.25006918902638 22.13550096277875 46-47 22.58235560692122 26.88632073598854 28.35070510329278 22.180618553797462 48-49 22.301392240521082 26.868626492978553 28.29572094635272 22.534260320147645 50-51 22.4792520366549 27.100918328427102 28.263273708814584 22.15655592610341 52-53 22.878031805781344 26.757370058061465 28.157354918226307 22.207243217930888 54-55 22.247831142046113 27.02778829693011 28.390581429192892 22.333799131830887 56-57 22.42935880319058 27.199323151259065 28.036405072453135 22.334912973097218 58-59 22.34789476268627 26.724207583169996 28.084425080243815 22.84347257389992 60-61 21.934036719463005 27.078051450122658 28.409897738959533 22.5780140914548 62-63 22.10095588694152 26.986908908941416 28.528519422817457 22.38361578129961 64-65 21.82943259685034 27.050623008623116 28.29599468046709 22.823949714059456 66-67 21.773611125238745 27.10757798519754 28.27527266553002 22.843538224033686 68-69 22.41280980182583 26.674735204631546 28.351930687267657 22.56052430627497 70-71 22.13005062830071 26.88834015426168 27.95501454138068 23.026594676056927 72-73 22.15047235078476 27.015143626012566 27.93823156278887 22.896152460413802 74-75 22.161625847741192 27.361229053317444 27.744877852302253 22.73226724663911 76-77 22.186602803420193 26.906742027592866 28.092948880971626 22.81370628801531 78-79 22.206155930760303 27.15080874137551 27.96411828242454 22.67891704543965 80-81 22.437406644961744 27.20237690045233 27.66264804769137 22.69756840689456 82-83 22.356154992093558 27.3237524706409 27.67992389780361 22.640168639461933 84-85 22.281070227256812 27.396979690754204 27.693908556551666 22.62804152543732 86-87 22.4044097521197 27.372541607996688 27.386139152377996 22.83690948750562 88-89 22.12883268997066 27.546890613739034 27.208195968295378 23.116080727994923 90-91 22.21490088114992 27.404214392604437 27.351254228547266 23.029630497698374 92-93 22.243876787047746 27.113688878159426 27.52029561154658 23.12213872324625 94-95 21.983771771959752 26.909320667908172 27.688064349540696 23.418843210591383 96-97 22.292480793979895 26.511477055571824 27.668425445744177 23.5276167047041 98-99 21.90496235706875 26.8357677483565 27.325063259841855 23.9342066347329 100-101 22.349362934789646 26.18509464776026 27.416406524614988 24.049135892835103 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1010.0 1 669.5 2 480.5 3 671.0 4 557.0 5 543.0 6 1047.5 7 2273.5 8 3681.5 9 4250.0 10 3566.5 11 2692.0 12 2557.5 13 2691.0 14 2879.5 15 3056.0 16 3291.5 17 3529.0 18 3778.5 19 4195.5 20 4767.5 21 5561.5 22 6626.0 23 8127.5 24 10057.5 25 12733.5 26 16277.0 27 20048.5 28 23338.0 29 26831.5 30 30807.0 31 36175.5 32 44740.0 33 48799.0 34 48853.5 35 52205.0 36 57214.5 37 61722.0 38 64655.5 39 67727.0 40 71102.0 41 74915.5 42 77196.5 43 79259.0 44 82341.5 45 87322.0 46 90542.5 47 92559.0 48 92978.0 49 90430.0 50 89759.5 51 88201.0 52 86678.0 53 88045.0 54 90931.5 55 86789.0 56 78786.5 57 72464.0 58 67439.5 59 59738.0 60 48004.5 61 39457.0 62 33188.0 63 24919.0 64 17442.0 65 13115.0 66 9811.5 67 7399.0 68 5583.0 69 4316.5 70 3411.0 71 2325.0 72 1563.0 73 1275.0 74 974.0 75 675.0 76 466.0 77 299.0 78 203.5 79 117.5 80 78.0 81 55.5 82 26.5 83 16.5 84 8.5 85 5.0 86 4.5 87 2.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009036133974181692 2 0.014590813733202472 3 0.008449372027806257 4 1.173523892750869E-4 5 8.60584188017304E-4 6 0.0015255810605761299 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0012322000873884126 14-15 0.0 16-17 0.002718663684872847 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002992485926514716 26-27 0.018776382284013907 28-29 0.007217171940417845 30-31 0.0038530701145320203 32-33 0.0 34-35 0.009114368900365084 36-37 8.019079933797605E-4 38-39 0.0024252827116851296 40-41 0.0017994033022179993 42-43 0.0027382224164186945 44-45 1.955873154584782E-5 46-47 1.7602858391263038E-4 48-49 7.823492618339127E-4 50-51 0.005007035275737042 52-53 0.009094810168819235 54-55 0.008566724417081345 56-57 0.01723124249189193 58-59 0.023411801660379837 60-61 0.003989981235352955 62-63 0.01973476012976045 64-65 0.021103871337969794 66-67 0.013339054914268211 68-69 0.031196176815627273 70-71 0.016488010693149712 72-73 0.005711149611387563 74-75 0.01640977576696632 76-77 0.020536668123140207 78-79 0.026306493929165318 80-81 0.011774356390600387 82-83 0.03489277707779251 84-85 0.022375188888449905 86-87 0.031078824426352184 88-89 0.02194489679444125 90-91 0.02771472260046636 92-93 0.024115915996030357 94-95 0.014199639102285515 96-97 0.014414785149289842 98-99 0.010913772202583083 100-101 0.010463921377028583 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2556403.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.86904686375841 #Duplication Level Percentage of deduplicated Percentage of total 1 73.69291275681684 37.48688232553376 2 14.7301827825506 14.986207165545876 3 5.026806141550873 7.671265115687399 4 2.217448211628384 4.511979079811263 5 1.19492241636725 3.0392282198370544 6 0.7004952055365379 2.138011406296574 7 0.44686829230552266 1.5912234872252133 8 0.31731728755206634 1.2913302376933415 9 0.23673221892168686 1.0838108104639925 >10 1.2769030298312685 12.078876670639167 >50 0.09908792019858223 3.4739827251151487 >100 0.05277414713265036 4.922649117617178 >500 0.0040059046898228414 1.4070771012148031 >1k 0.0033125750319617083 3.223392384566301 >5k 1.5407325730054457E-4 0.6277885356604723 >10k+ 7.703662865027229E-5 0.4662956170926317 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11899 0.46545869332808637 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9820 0.3841334875604512 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6200 0.24252827116851297 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3929 0.15369251248727214 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3911 0.15298839815162163 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3538 0.13839758441841918 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3528 0.1380064097875022 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3303 0.1292049805918707 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2937 0.11488798910031009 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2930 0.11461416685866821 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2704 0.105773620199945 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.911746309169564E-5 0.0 3 0.0 0.0 0.0 3.911746309169564E-5 0.0 4 0.0 0.0 0.0 3.911746309169564E-5 0.0 5 0.0 0.0 0.0 3.911746309169564E-5 0.0 6 0.0 0.0 0.0 3.911746309169564E-5 3.911746309169564E-5 7 0.0 0.0 0.0 3.911746309169564E-5 3.911746309169564E-5 8 0.0 0.0 0.0 3.911746309169564E-5 3.911746309169564E-5 9 0.0 0.0 0.0 7.823492618339128E-5 3.911746309169564E-5 10-11 0.0 0.0 0.0 7.823492618339128E-5 3.911746309169564E-5 12-13 0.0 0.0 0.0 1.1735238927508691E-4 1.1735238927508691E-4 14-15 0.0 0.0 0.0 1.3691112082093472E-4 1.3691112082093472E-4 16-17 0.0 0.0 0.0 1.5646985236678255E-4 1.5646985236678255E-4 18-19 0.0 0.0 0.0 2.1514604700432599E-4 1.5646985236678255E-4 20-21 0.0 0.0 0.0 2.3470477855017381E-4 1.9558731545847818E-4 22-23 0.0 0.0 0.0 3.324984362794129E-4 1.9558731545847818E-4 24-25 0.0 0.0 0.0 6.649968725588258E-4 1.9558731545847818E-4 26-27 0.0 0.0 0.0 0.0014277874028468906 2.5426351009602164E-4 28-29 0.0 0.0 0.0 0.004342038403178216 2.7382224164186945E-4 30-31 0.0 0.0 0.0 0.012556705652434299 2.9338097318771725E-4 32-33 0.0 0.0 0.0 0.03084411964780201 3.520571678252607E-4 34-35 0.0 0.0 0.0 0.05805031522807633 3.520571678252607E-4 36-37 0.0 0.0 0.0 0.10017982297783252 3.520571678252607E-4 38-39 0.0 0.0 0.0 0.1696328786971381 3.520571678252607E-4 40-41 0.0 0.0 0.0 0.24035725196692384 3.520571678252607E-4 42-43 0.0 0.0 0.0 0.31207912054554776 3.9117463091695637E-4 44-45 0.0 0.0 0.0 0.4013647300523431 4.498508255544998E-4 46-47 0.0 0.0 0.0 0.5032852801377561 4.889682886461955E-4 48-49 0.0 0.0 0.0 0.6089415479484259 5.476444832837389E-4 50-51 0.0 0.0 0.0 0.729482010465486 5.476444832837389E-4 52-53 0.0 0.0 0.0 0.8380525292764873 6.258794094671302E-4 54-55 0.0 0.0 0.0 0.9566762361020543 6.258794094671302E-4 56-57 0.0 0.0 0.0 1.0884238517948852 6.258794094671302E-4 58-59 0.0 0.0 0.0 1.2282687823476972 6.258794094671302E-4 60-61 0.0 0.0 0.0 1.3849928982245756 6.258794094671302E-4 62-63 0.0 0.0 0.0 1.5335023468521982 6.258794094671302E-4 64-65 0.0 0.0 0.0 1.682226941526825 6.258794094671302E-4 66-67 0.0 0.0 0.0 1.858568465144189 6.258794094671302E-4 68-69 0.0 0.0 0.0 2.048405513528188 6.258794094671302E-4 70-71 0.0 0.0 0.0 2.2448142957115915 6.649968725588258E-4 72-73 0.0 0.0 0.0 2.4531343454064167 7.236730671963693E-4 74-75 0.0 0.0 0.0 2.6556063343690335 7.823492618339127E-4 76-77 0.0 0.0 0.0 2.880531747146283 7.823492618339127E-4 78-79 0.0 0.0 0.0 3.1313529204902357 7.823492618339127E-4 80-81 0.0 0.0 0.0 3.3913666976607364 8.019079933797605E-4 82-83 0.0 0.0 0.0 3.658538970577018 8.997016511089997E-4 84-85 0.0 0.0 0.0 3.9319504788564243 8.997016511089997E-4 86-87 0.0 0.0 0.0 4.209645349344372 8.997016511089997E-4 88-89 0.0 0.0 0.0 4.502087503417888 8.997016511089997E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5785 0.0 56.577415 1 GTATCAA 10085 0.0 41.026974 1 ATCAACG 12915 0.0 31.62553 3 TATCAAC 13025 0.0 31.471527 2 TCAACGC 13280 0.0 30.79026 4 CAACGCA 13475 0.0 30.379932 5 AACGCAG 13915 0.0 29.419302 6 TGGTATC 2485 0.0 26.95116 2 ACGCAGA 15675 0.0 25.874212 7 CGCAGAG 15820 0.0 25.546999 8 GTATAGG 640 0.0 25.23633 1 GTGGTAT 2620 0.0 25.202333 1 TACATGG 9450 0.0 23.322277 2 GTACATG 9885 0.0 23.211205 1 GAGTACT 10645 0.0 23.200186 12-13 TAAGGTG 1705 0.0 23.118626 5 GCAGAGT 17550 0.0 22.839266 9 GTACTTT 11205 0.0 22.039833 14-15 GGACCGA 735 0.0 21.968473 6 GTAAGGT 1680 0.0 21.766558 4 >>END_MODULE