FastQCFastQC Report
Thu 26 May 2016
SRR938259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881627
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79260.4212312004451467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65290.34698694268311414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40670.21614273179540897No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25410.13504270506322452No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23070.12260665902434435No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22740.12085285765988689No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA20730.11017061298546418No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA19200.10203935211388866No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC19140.10172047913853277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36950.053.328241
GTATCAA64050.041.7893261
TATCAAC81750.032.5560652
ATCAACG81800.032.3037643
TCAACGC82900.031.9324574
TGGTATC15200.031.8924732
CAACGCA84700.031.2538435
AACGCAG88050.030.0591456
GTGGTAT17550.029.9045451
ACGCAGA97600.026.916727
TAAGGTG10250.026.8927845
CGCAGAG98250.026.6419438
GTATAGG3850.026.024371
GTACATG64450.024.7255061
TACATGG63350.024.3819472
GCAGAGT107150.024.1617329
GTAAGGT10800.023.3229664
GAGTACT69150.022.73898712-13
AAGGTAA10600.022.0552431
AGGTAAG11050.021.9350972
Produced by FastQC (version 0.11.5)