FastQCFastQC Report
Thu 26 May 2016
SRR938259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881627
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76230.4051281151896736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66850.3552776400423676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41390.2199692074996798No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24710.13132252035073902No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23300.12382900542987532No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23210.12335069596684148No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21050.11187126885402898No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20840.11075521344028331No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19290.10251766157692252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.052.0383641
GTATCAA66000.040.572331
TATCAAC83000.032.1477282
ATCAACG83350.031.7785573
TCAACGC84650.031.2896924
CAACGCA85300.030.9946865
AACGCAG88600.029.8045756
ACGCAGA101400.025.9485847
CGCAGAG102700.025.4813828
GTGGTAT18350.024.1052911
GCAGAGT113100.022.9283039
TGGTATC17850.022.915312
GAGTACT72950.022.13581312-13
GTGTAAG6800.021.6829471
GTACATG63450.021.6636541
GTACTTT74850.021.54218914-15
TACATGG62500.021.003642
TAAGGTG11900.020.7786755
CAGAGTA110100.020.57656710-11
AGAGTAC101350.020.12710210-11
Produced by FastQC (version 0.11.5)