##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938259_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1881627 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46093248024183 33.0 31.0 34.0 31.0 34.0 2 32.69267554090157 34.0 31.0 34.0 31.0 34.0 3 32.7538215597459 34.0 31.0 34.0 31.0 34.0 4 36.21718757224466 37.0 35.0 37.0 35.0 37.0 5 36.14148128189062 37.0 35.0 37.0 35.0 37.0 6 36.19399966093174 37.0 37.0 37.0 35.0 37.0 7 36.167020881396795 37.0 37.0 37.0 35.0 37.0 8 36.15697903994788 37.0 36.0 37.0 35.0 37.0 9 37.84665239178647 39.0 38.0 39.0 35.0 39.0 10-11 37.8154881387225 39.0 38.0 39.0 35.0 39.0 12-13 37.73263484208081 39.0 38.0 39.0 35.0 39.0 14-15 38.96597386198221 40.0 38.0 41.0 35.5 41.0 16-17 38.86784920709577 40.0 38.0 41.0 35.0 41.0 18-19 39.11783844513286 40.0 38.5 41.0 36.0 41.0 20-21 39.08360371104369 40.0 39.0 41.0 36.0 41.0 22-23 38.97745249191259 40.0 38.5 41.0 35.0 41.0 24-25 38.85948862340942 40.0 38.0 41.0 35.0 41.0 26-27 38.6825412262898 40.0 38.0 41.0 34.5 41.0 28-29 38.55315665644679 40.0 38.0 41.0 34.0 41.0 30-31 38.30227032243904 40.0 38.0 41.0 34.0 41.0 32-33 38.07039678958688 40.0 37.5 41.0 33.5 41.0 34-35 37.973585891358915 40.0 37.0 41.0 33.0 41.0 36-37 37.90960137157896 40.0 37.0 41.0 33.0 41.0 38-39 37.707500742708305 40.0 37.0 41.0 33.0 41.0 40-41 37.47168035960368 40.0 37.0 41.0 32.5 41.0 42-43 37.19605400007546 40.0 36.0 41.0 31.5 41.0 44-45 36.90147941116916 39.5 36.0 41.0 31.0 41.0 46-47 36.515705822673674 39.0 35.5 41.0 30.5 41.0 48-49 36.07536270472309 39.0 35.0 40.5 29.5 41.0 50-51 35.66287526698969 39.0 35.0 40.0 28.0 41.0 52-53 35.093289743397605 38.0 34.0 40.0 26.5 41.0 54-55 34.675406443466215 38.0 34.0 40.0 26.0 41.0 56-57 34.256196100502386 37.5 33.5 40.0 25.0 41.0 58-59 33.700256480163176 37.0 33.0 40.0 23.0 41.0 60-61 33.18227895326757 36.0 32.0 39.0 22.0 40.5 62-63 32.57795806501501 36.0 31.5 39.0 19.5 40.0 64-65 31.96154763935679 35.0 31.0 38.5 17.0 40.0 66-67 31.354030049526287 35.0 30.5 38.0 10.5 40.0 68-69 30.69989429360867 34.5 29.5 37.5 7.5 39.5 70-71 30.050970250745763 34.0 29.0 37.0 4.5 39.0 72-73 29.360832938728027 34.0 28.0 36.0 2.0 39.0 74-75 28.690878160230483 34.0 26.5 35.5 2.0 38.5 76-77 27.429392488521902 31.5 26.0 34.5 2.0 36.0 78-79 28.077857354300292 33.0 26.5 35.0 2.0 37.0 80-81 27.799716149906438 33.0 26.0 35.0 2.0 36.5 82-83 27.23129637276676 33.0 26.0 35.0 2.0 36.0 84-85 26.562637281459075 32.5 24.5 35.0 2.0 35.5 86-87 25.841759285979634 32.0 22.0 35.0 2.0 35.0 88-89 25.011967834220066 31.5 19.0 34.0 2.0 35.0 90-91 24.32983928270587 31.0 14.0 34.0 2.0 35.0 92-93 23.374417458933145 30.5 4.5 34.0 2.0 35.0 94-95 22.445154113966264 30.0 2.0 34.0 2.0 35.0 96-97 21.367509341649537 29.0 2.0 34.0 2.0 35.0 98-99 19.922720602967537 28.5 2.0 34.0 2.0 35.0 100-101 16.166578976598444 13.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23683485063507703 1101 2 0.20870076004892724 1101 3 0.22737265181210375 1101 4 0.05232316539952819 1101 5 -0.04491159968109315 1101 6 0.06924810614967214 1101 7 0.06934902579546787 1101 8 0.18019661267724985 1101 9 0.14959826431574186 1101 10-11 0.20840430664760845 1101 12-13 0.23435566074181935 1101 14-15 0.34587315267430796 1101 16-17 0.3798555930139713 1101 18-19 0.4119006009157715 1101 20-21 0.2765065717436386 1101 22-23 0.3727219502292982 1101 24-25 0.4320703135121704 1101 26-27 0.276675063968689 1101 28-29 0.30020341215192303 1101 30-31 0.2932461835303002 1101 32-33 0.2634322990371274 1101 34-35 0.21669547108474774 1101 36-37 0.3599522776587776 1101 38-39 0.429764071589787 1101 40-41 0.5412840565541828 1101 42-43 0.6799364695110981 1101 44-45 0.6385988557160331 1101 46-47 0.6347673549378143 1101 48-49 0.557639504135544 1101 50-51 0.49650649302643757 1101 52-53 0.6069276906592478 1101 54-55 0.8992445695899747 1101 56-57 0.9799151895628739 1101 58-59 1.0988938171613754 1101 60-61 1.0066707989694308 1101 62-63 1.089170747866298 1101 64-65 1.2256302285692158 1101 66-67 1.4824313892736427 1101 68-69 1.7330048325734602 1101 70-71 1.8252192033089152 1101 72-73 1.95813245158935 1101 74-75 1.984021284098855 1101 76-77 1.9020649392421127 1101 78-79 1.999955496343965 1101 80-81 2.236922338723229 1101 82-83 2.3374267137075293 1101 84-85 2.43562958731291 1101 86-87 2.8285454987042264 1101 88-89 3.128173905373597 1101 90-91 3.430021762096377 1101 92-93 3.5640523671056634 1101 94-95 3.7885761680447096 1101 96-97 3.836216384251049 1101 98-99 3.9436776347677167 1101 100-101 4.078520317789341 1102 1 0.15465341051964998 1102 2 0.14328562964792724 1102 3 0.10340641782966742 1102 4 0.05006875164503555 1102 5 -0.02727535148258653 1102 6 0.002781460048154827 1102 7 0.05338083975594543 1102 8 0.10737353988478304 1102 9 0.2214599979578793 1102 10-11 0.19920405431648192 1102 12-13 0.21230055756515753 1102 14-15 0.2967779376624051 1102 16-17 0.30550359925533144 1102 18-19 0.31265049441767445 1102 20-21 0.2772414494218083 1102 22-23 0.33924094769456303 1102 24-25 0.4512375228702865 1102 26-27 0.3583387029882843 1102 28-29 0.24569556393578296 1102 30-31 0.3665254435766201 1102 32-33 0.292462007517976 1102 34-35 0.2977933644626063 1102 36-37 0.42160794360240317 1102 38-39 0.5296254200434021 1102 40-41 0.8254362827316655 1102 42-43 0.9258605710507126 1102 44-45 0.7163047234755879 1102 46-47 0.7962592749556947 1102 48-49 0.8182356568203559 1102 50-51 0.9277901256153456 1102 52-53 0.8301321980178216 1102 54-55 0.9212676417295782 1102 56-57 1.2648428932680247 1102 58-59 1.2816345559072673 1102 60-61 1.185884247271737 1102 62-63 1.523900063161058 1102 64-65 1.5077777606696117 1102 66-67 1.548377990066541 1102 68-69 1.8209212564571082 1102 70-71 1.9680341724530344 1102 72-73 2.36097741973785 1102 74-75 2.5327904761985742 1102 76-77 2.513540088869899 1102 78-79 2.6509330754341036 1102 80-81 2.6617842584310054 1102 82-83 2.8237229383703664 1102 84-85 2.9794272360307694 1102 86-87 2.8816296999358535 1102 88-89 3.2121900914522925 1102 90-91 3.2995997440023075 1102 92-93 3.7142485521071436 1102 94-95 3.8517187269695796 1102 96-97 4.124406765310223 1102 98-99 4.106586265112412 1102 100-101 4.067492460171991 1103 1 0.2644793746117955 1103 2 0.255974575631555 1103 3 0.2758763426068285 1103 4 0.16549623565283866 1103 5 0.1625378745043875 1103 6 0.20149203607640231 1103 7 0.22320111498558504 1103 8 0.1935822222020036 1103 9 0.2794748013674919 1103 10-11 0.2618082084228206 1103 12-13 0.316193022604935 1103 14-15 0.41667612557419176 1103 16-17 0.5331794515798833 1103 18-19 0.3781899006117371 1103 20-21 0.36555717510732677 1103 22-23 0.39550464811411246 1103 24-25 0.43578020325205813 1103 26-27 0.43099537086074946 1103 28-29 0.4676624572294159 1103 30-31 0.41825621815853964 1103 32-33 0.5236304040316853 1103 34-35 0.4531321609590151 1103 36-37 0.501543804769419 1103 38-39 0.5536979654074372 1103 40-41 0.630231390928266 1103 42-43 0.7690365640509356 1103 44-45 0.8708806249593266 1103 46-47 0.8672788489712033 1103 48-49 1.0073157461107556 1103 50-51 1.1905935444980429 1103 52-53 1.1375773422335698 1103 54-55 1.2152303363953436 1103 56-57 1.1301559816710949 1103 58-59 1.3185337084522573 1103 60-61 1.3640615851932623 1103 62-63 1.4335870520523173 1103 64-65 1.4771549075195622 1103 66-67 1.575693418941853 1103 68-69 1.6265136556421176 1103 70-71 1.5855915016557525 1103 72-73 1.6040661474423032 1103 74-75 1.740020433006503 1103 76-77 1.7000501417701344 1103 78-79 1.7087739666067812 1103 80-81 1.936722113791678 1103 82-83 2.109920258421443 1103 84-85 2.0884215131587602 1103 86-87 2.402010668217354 1103 88-89 2.4296688896182523 1103 90-91 2.7657289832097014 1103 92-93 2.884966987422608 1103 94-95 2.9899561535013213 1103 96-97 3.0940007227488735 1103 98-99 3.3776451046462945 1103 100-101 3.183520134633021 1104 1 0.1660096628349308 1104 2 0.19885990986193036 1104 3 0.1815049439460097 1104 4 0.12159795907578541 1104 5 0.0162564429874692 1104 6 0.02487453273424478 1104 7 0.11951734555248805 1104 8 0.10304814378234539 1104 9 0.1571757151325457 1104 10-11 0.1963725823154121 1104 12-13 0.21016974204049177 1104 14-15 0.2992746431346589 1104 16-17 0.3253018891373358 1104 18-19 0.3020017305487741 1104 20-21 0.29911817639199256 1104 22-23 0.2768453250136389 1104 24-25 0.28778491852332877 1104 26-27 0.2652652577928336 1104 28-29 0.44001424952548973 1104 30-31 0.5016322044941077 1104 32-33 0.48402437277633936 1104 34-35 0.5468702894081048 1104 36-37 0.616697123583819 1104 38-39 0.7035339748843796 1104 40-41 0.7365022522337483 1104 42-43 0.6923673696199444 1104 44-45 0.784411873056257 1104 46-47 0.9562397203394113 1104 48-49 1.1166576235775025 1104 50-51 1.3603494726611984 1104 52-53 1.431747123898532 1104 54-55 1.3748307418042813 1104 56-57 1.3341259007923014 1104 58-59 1.5284379530663799 1104 60-61 1.5016062802230934 1104 62-63 1.5727926767387643 1104 64-65 1.7404412573022086 1104 66-67 1.8580355110027398 1104 68-69 1.894604811753556 1104 70-71 1.7652825585898988 1104 72-73 2.069678161234325 1104 74-75 2.099466707623211 1104 76-77 2.11695182285424 1104 78-79 2.2899500005010545 1104 80-81 2.2726899043709956 1104 82-83 2.4598127438565918 1104 84-85 2.7167448224324175 1104 86-87 2.5785665482418274 1104 88-89 2.8430671038470052 1104 90-91 3.081149022242638 1104 92-93 3.150255007559661 1104 94-95 3.4358559554367254 1104 96-97 3.5398664506145003 1104 98-99 3.7580161205175635 1104 100-101 3.3034474481173604 1105 1 0.0731390009770081 1105 2 0.07551735202800103 1105 3 0.09634240306004926 1105 4 0.01813447295143078 1105 5 -0.020695801117923907 1105 6 -0.015910791576544625 1105 7 0.009122179409132514 1105 8 -0.0024482121305453575 1105 9 0.045831830935505025 1105 10-11 0.03701268214744147 1105 12-13 0.1198982469754668 1105 14-15 0.21891010478952921 1105 16-17 0.1466254616936311 1105 18-19 0.10137054818886071 1105 20-21 0.15626174018773753 1105 22-23 0.189073894765734 1105 24-25 0.25908628268506106 1105 26-27 0.19662033829570902 1105 28-29 0.24932140902065214 1105 30-31 0.2157315329886771 1105 32-33 0.3961935836858146 1105 34-35 0.2637253692286947 1105 36-37 0.35836078819407646 1105 38-39 0.43262669885484684 1105 40-41 0.4520500729454824 1105 42-43 0.5268648526685595 1105 44-45 0.6423895309337198 1105 46-47 0.5506052698317276 1105 48-49 0.776373676801235 1105 50-51 0.7935023755815109 1105 52-53 0.9435034155947122 1105 54-55 1.0837170675737937 1105 56-57 1.1132971899435518 1105 58-59 1.1294609037876242 1105 60-61 0.913319553815505 1105 62-63 1.0690032628084012 1105 64-65 1.0838272153198147 1105 66-67 1.1895137099326405 1105 68-69 1.4507282563130808 1105 70-71 1.6088087262874993 1105 72-73 1.6866689817645693 1105 74-75 1.6366087976821682 1105 76-77 1.652049444270368 1105 78-79 1.7323732877255331 1105 80-81 2.0988328008914756 1105 82-83 2.171483818087893 1105 84-85 2.4223684587141783 1105 86-87 2.415284700175903 1105 88-89 2.574734773473427 1105 90-91 2.6388246664225363 1105 92-93 2.7819393590677137 1105 94-95 2.9182731157369872 1105 96-97 3.2549480997259614 1105 98-99 3.372474297095792 1105 100-101 3.641949947329323 1106 1 0.08204314521044154 1106 2 0.12828914867775865 1106 3 0.06734134854609408 1106 4 0.11173939324822868 1106 5 -0.015391858439436135 1106 6 0.045808367091623836 1106 7 0.09949152820355067 1106 8 0.1789701251436071 1106 9 0.15039197952785344 1106 10-11 0.1602065788998246 1106 12-13 0.12531391563143046 1106 14-15 0.09254242196069384 1106 16-17 0.041365505116303325 1106 18-19 0.09185559259433518 1106 20-21 0.01749359432034936 1106 22-23 -0.012151633563682651 1106 24-25 -0.016452226922410773 1106 26-27 0.008227290788788366 1106 28-29 0.12840572980034182 1106 30-31 0.26033052636642395 1106 32-33 0.3722089935472823 1106 34-35 0.15628099371927817 1106 36-37 0.28578372127949336 1106 38-39 0.1799115014968251 1106 40-41 0.1947217772052241 1106 42-43 0.1943881918731094 1106 44-45 0.23782672524792048 1106 46-47 0.5637846133951925 1106 48-49 0.484750948215968 1106 50-51 0.6486288372230149 1106 52-53 0.5916459792224913 1106 54-55 0.5476984051738398 1106 56-57 0.7133866987782227 1106 58-59 0.7746167983413486 1106 60-61 0.7876055800009922 1106 62-63 0.6998843229822924 1106 64-65 0.8331181235143532 1106 66-67 1.043381893767652 1106 68-69 0.9423014285049014 1106 70-71 1.026384887229252 1106 72-73 1.2023528760007238 1106 74-75 1.394564551634037 1106 76-77 1.3749626174994134 1106 78-79 1.655995776852258 1106 80-81 1.6037897207631389 1106 82-83 1.9666536063591629 1106 84-85 2.065181148992483 1106 86-87 2.1745390685335693 1106 88-89 2.335692722888769 1106 90-91 2.404123122042993 1106 92-93 2.14881881730237 1106 94-95 2.5739600640299507 1106 96-97 3.135208904652405 1106 98-99 3.254749531000048 1106 100-101 2.81957360301611 1107 1 0.16867761702828687 1107 2 0.17514665147730568 1107 3 0.11309069531727545 1107 4 0.12784848246062097 1107 5 -0.04381897676717017 1107 6 0.10156611251902348 1107 7 0.10376084511284489 1107 8 0.08519679554301263 1107 9 0.126747429322549 1107 10-11 0.1338791246258424 1107 12-13 0.19445118700134145 1107 14-15 0.2819146224086211 1107 16-17 0.282856033298458 1107 18-19 0.11879544888399352 1107 20-21 0.20015853459657507 1107 22-23 0.2047261659883901 1107 24-25 0.24491512096858514 1107 26-27 0.20630726465964244 1107 28-29 0.053202762275141424 1107 30-31 0.2331573740707924 1107 32-33 0.33005240526807 1107 34-35 0.2530171825957268 1107 36-37 0.44606974218282147 1107 38-39 0.2712468878350123 1107 40-41 0.18718631621231197 1107 42-43 0.3175276562254723 1107 44-45 0.45616845723747446 1107 46-47 0.5593753188823101 1107 48-49 0.758809598831867 1107 50-51 0.7609142048975173 1107 52-53 0.8177506358107749 1107 54-55 1.0604655291006821 1107 56-57 1.019354410611001 1107 58-59 1.016346454929625 1107 60-61 1.175034424719179 1107 62-63 1.2462868985701974 1107 64-65 1.2863597715173398 1107 66-67 1.6278233831293534 1107 68-69 1.775967208646744 1107 70-71 1.8409437244819564 1107 72-73 1.9032090723426442 1107 74-75 1.8321053784350845 1107 76-77 1.8895319530723889 1107 78-79 1.758062779465174 1107 80-81 2.0203222422263707 1107 82-83 2.392115532450987 1107 84-85 2.2675596017733675 1107 86-87 2.58127899014611 1107 88-89 2.7446956157592446 1107 90-91 2.5267784971829705 1107 92-93 2.7746936773247626 1107 94-95 2.812569386538346 1107 96-97 3.0620239475788686 1107 98-99 3.065260682549134 1107 100-101 2.9558826269056198 1108 1 0.19001687962860103 1108 2 0.1554826183809439 1108 3 0.11667638343086395 1108 4 0.09526302833154432 1108 5 0.0822607123819239 1108 6 0.11384034236150598 1108 7 0.12755632273994166 1108 8 0.1626462897382055 1108 9 0.14305975881256217 1108 10-11 0.196924881042996 1108 12-13 0.16406244912373324 1108 14-15 0.32218640537056586 1108 16-17 0.30136476789032685 1108 18-19 0.29065302450579367 1108 20-21 0.35956390279857686 1108 22-23 0.2746330824708565 1108 24-25 0.2601530508528924 1108 26-27 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58-59 1.0365066606692608 2316 60-61 1.1304738583313636 2316 62-63 1.2693022357240906 2316 64-65 1.560908343745787 2316 66-67 1.6606776362527782 2316 68-69 2.010610499053616 2316 70-71 1.9376610438285091 2316 72-73 2.0638793454910953 2316 74-75 2.4420910650389516 2316 76-77 2.4180537621319615 2316 78-79 2.570466716403889 2316 80-81 2.523415129717314 2316 82-83 2.773100261168903 2316 84-85 3.020245593583308 2316 86-87 3.053625865626426 2316 88-89 3.480940071548279 2316 90-91 3.66892232279832 2316 92-93 3.8529500778693055 2316 94-95 4.453958087875545 2316 96-97 4.871952202195388 2316 98-99 5.148064639798324 2316 100-101 5.12617944804169 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 15.0 5 38.0 6 77.0 7 212.0 8 518.0 9 973.0 10 1670.0 11 2790.0 12 4142.0 13 6285.0 14 8498.0 15 11774.0 16 15783.0 17 21204.0 18 22188.0 19 19071.0 20 19211.0 21 20474.0 22 21676.0 23 23742.0 24 26579.0 25 29441.0 26 33379.0 27 38865.0 28 46216.0 29 55051.0 30 65263.0 31 77459.0 32 94068.0 33 116794.0 34 150184.0 35 202978.0 36 274539.0 37 316116.0 38 148550.0 39 5799.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66428627990563 18.286195935751348 14.742454269629421 23.307063514713597 2 16.258992805755394 19.350365293284398 39.210520021367465 25.180121879592743 3 19.992442525727036 22.729927651267737 29.17211642762124 28.10551339538399 4 13.529807860433749 16.173035622323756 35.4026172392553 34.8945392779872 5 14.359543543185632 36.453866671490815 33.681913640975765 15.50467614434779 6 33.83332615869139 34.993917497995085 16.280697502746293 14.892058840567232 7 29.80287804118457 31.931355151685214 19.403792568877893 18.861974238252323 8 27.327549325715804 34.32453331561815 18.66184813658407 19.68606922208198 9 26.203386749871253 16.693584860336294 19.5700316800301 37.53299670976235 10-11 25.81021637125743 25.954320383370348 26.08447370281145 22.150989542560772 12-13 26.647656522785866 23.039901106861244 26.84982198916151 23.462620381191385 14-15 23.13112535056098 24.663017696918676 25.14573823611162 27.060118716408727 16-17 22.839622305589792 27.809177908267685 26.14035619174257 23.21084359439995 18-19 22.863856651716837 26.06018621118851 28.539795613051893 22.536161524042754 20-21 23.100858990650114 25.204650018308623 29.125990432747827 22.568500558293433 22-23 22.986583646073345 25.11554528899675 29.0204485864984 22.8774224784315 24-25 22.9279435125352 25.334884123848532 28.89954990936007 22.8376224542562 26-27 22.357520705344992 25.71358567200225 28.863195109908535 23.065698512744223 28-29 22.602619966656516 26.131055729961357 28.612976960896074 22.65334734248605 30-31 22.612531601640494 25.604516729404924 28.618769819948376 23.164181849006205 32-33 22.319168464313066 25.60164687262672 28.67685784695904 23.402326816101173 34-35 23.126656498790403 25.78197107443837 28.438508133902374 22.652864292868856 36-37 23.146889624250676 25.313248443423692 28.35489328706453 23.1849686452611 38-39 22.64869011955162 25.70270825466114 28.344765328089967 23.303836297697273 40-41 23.14113345899979 25.556523197313968 28.399565119293218 22.90277822439302 42-43 22.816397392196095 25.607511852868964 28.290893494122926 23.285197260812016 44-45 22.807680022449492 25.909233682529948 28.383035964363557 22.900050330657002 46-47 23.323411952372055 26.149796465983023 27.56997735735546 22.956814224289467 48-49 23.05858174866751 26.17862094878528 27.509065292961886 23.25373200958532 50-51 23.174173202234023 26.48170971186106 27.4424208411125 22.901696244792404 52-53 23.517253950969028 26.110833868774204 27.43498047168754 22.936931708569233 54-55 23.073595351257183 26.346480997562217 27.580838285164916 22.999085366015688 56-57 23.127245729360812 26.451682506681717 27.37391629690688 23.04715546705059 58-59 23.05257630763164 26.02210746338142 27.391879474518593 23.53343675446834 60-61 22.77568827403093 26.3690146878207 27.602096483521972 23.253200554626396 62-63 22.90432694683909 26.3589170436011 27.69175293509287 23.045003074466937 64-65 22.56834112180576 26.45229367988448 27.554664128437782 23.42470106987198 66-67 22.562123098786316 26.45229367988448 27.49240417999954 23.493179041329658 68-69 23.12055998822286 25.997323570068414 27.635015951756237 23.247100489952494 70-71 22.838373386436313 26.21598223239781 27.248785226827632 23.69685915433824 72-73 22.881055299763265 26.346886589873176 27.176301991920287 23.595756118443273 74-75 22.78870892052463 26.64553070295016 27.082599261171314 23.483161115353894 76-77 22.892581792246816 26.31868590321036 27.36929263876422 23.419439665778604 78-79 22.93711771780486 26.39845729254523 27.39190604726654 23.272518942383375 80-81 23.0876032917301 26.462771929255812 27.091297971513445 23.358326807500646 82-83 22.928079898468738 26.55754075812558 27.20133206631845 23.313047277087225 84-85 22.909603543896107 26.66595802917283 27.068313540202332 23.356124886728725 86-87 23.08765677100883 26.673021302468264 26.805198435771054 23.43412349075185 88-89 22.89515934879761 26.84897166122723 26.511790062536306 23.74407892743886 90-91 22.842480423388402 26.76141672273651 26.77085027386529 23.6252525800098 92-93 22.958615070893433 26.32729547354497 26.93583797426376 23.778251481297836 94-95 22.684496630973257 26.215059152281285 27.068310315570066 24.032133901175392 96-97 23.053610575964925 25.875187670231846 27.00963263136916 24.061569122434065 98-99 22.734633378453857 26.20782439877829 26.603891206918266 24.45365101584958 100-101 23.1833939457714 25.52506421304541 26.771724682947255 24.51981715823593 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 592.0 1 423.0 2 394.5 3 522.5 4 430.5 5 379.5 6 740.0 7 1643.5 8 2644.0 9 3047.5 10 2519.5 11 1817.0 12 1647.5 13 1763.0 14 1930.0 15 2085.0 16 2265.5 17 2465.0 18 2657.0 19 2924.0 20 3279.0 21 3751.0 22 4451.5 23 5401.0 24 6685.5 25 8295.5 26 10264.5 27 12614.5 28 14738.0 29 16646.5 30 18712.0 31 21942.0 32 26984.0 33 29214.5 34 28961.0 35 30469.5 36 33398.5 37 36298.0 38 38935.0 39 42064.0 40 45610.5 41 49874.5 42 52314.0 43 54287.0 44 57479.5 45 61644.5 46 64288.0 47 66755.0 48 69182.0 49 68906.0 50 69123.5 51 68874.0 52 69023.5 53 71286.0 54 73207.5 55 70434.0 56 66135.5 57 62278.0 58 58818.0 59 53489.0 60 43992.0 61 36429.5 62 30495.5 63 22889.0 64 16597.0 65 13006.0 66 9924.0 67 7583.5 68 5869.5 69 4443.5 70 3323.0 71 2294.5 72 1667.5 73 1286.5 74 977.5 75 743.5 76 492.5 77 317.5 78 226.0 79 139.0 80 74.5 81 52.5 82 36.5 83 22.5 84 16.5 85 9.0 86 4.0 87 1.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013924119923874392 3 0.002444692811061916 4 0.001381782893208909 5 0.11660121798847486 6 0.0 7 0.0 8 1.062909917853007E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.923002274095769E-4 24-25 3.188729753559021E-4 26-27 0.0057397135564062375 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01124027238129555 36-37 7.174641945507797E-4 38-39 0.007360651181132074 40-41 0.010177362463442542 42-43 0.006404032255064367 44-45 0.0037201847124855246 46-47 2.6572747946325174E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.503279342824056E-4 70-71 0.0 72-73 2.6572747946325174E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3286373973162588E-4 82-83 0.0020726743398133637 84-85 0.004357930663197329 86-87 0.003241875249451671 88-89 0.0 90-91 0.0023915473151692656 92-93 0.0 94-95 2.6572747946325174E-5 96-97 1.062909917853007E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1881627.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.51188436285395 #Duplication Level Percentage of deduplicated Percentage of total 1 79.02741165261563 45.45015360460887 2 12.327080175857304 14.179072192110691 3 3.665556732626902 6.324392247969575 4 1.5707309007843646 3.613427757242872 5 0.8786785261902526 2.526722889518837 6 0.534875736442692 1.8457026901673075 7 0.3648049704398876 1.468643489245322 8 0.25107708135262663 1.1551932855132108 9 0.18413759202028307 0.9531089909212408 >10 1.079401157114684 11.303729270372 >50 0.07153081101732457 2.8117170333131196 >100 0.03889560445200863 4.0884079750550155 >500 0.003232810603393202 1.319430252840135 >1k 0.00240151644823495 2.2002368001604617 >5k 1.8473203447961155E-4 0.7600615209612953 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7623 0.4051281151896736 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6685 0.3552776400423676 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4139 0.2199692074996798 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2471 0.13132252035073902 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2330 0.12382900542987532 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2321 0.12335069596684148 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2105 0.11187126885402898 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2084 0.11075521344028331 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1929 0.10251766157692252 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.314549589265035E-5 7 0.0 0.0 0.0 0.0 5.314549589265035E-5 8 0.0 0.0 0.0 0.0 1.062909917853007E-4 9 0.0 0.0 0.0 0.0 1.062909917853007E-4 10-11 0.0 0.0 0.0 0.0 1.3286373973162588E-4 12-13 0.0 0.0 0.0 5.314549589265035E-5 3.7201847124855244E-4 14-15 0.0 0.0 0.0 7.971824383897553E-5 5.048822109801783E-4 16-17 0.0 0.0 0.0 1.062909917853007E-4 6.11173202765479E-4 18-19 7.971824383897553E-5 0.0 0.0 1.062909917853007E-4 6.377459507118042E-4 20-21 1.5943648767795105E-4 0.0 0.0 1.062909917853007E-4 6.377459507118042E-4 22-23 1.5943648767795105E-4 0.0 0.0 1.3286373973162588E-4 6.377459507118042E-4 24-25 2.125819835706014E-4 0.0 0.0 3.188729753559021E-4 6.908914466044546E-4 26-27 2.125819835706014E-4 0.0 0.0 5.314549589265035E-4 6.908914466044546E-4 28-29 2.125819835706014E-4 0.0 0.0 0.0024978383069545664 6.908914466044546E-4 30-31 2.125819835706014E-4 0.0 0.0 0.008901870562018934 7.440369424971049E-4 32-33 2.125819835706014E-4 0.0 0.0 0.021550498584469717 8.503279342824056E-4 34-35 2.125819835706014E-4 0.0 0.0 0.041559777788052574 8.503279342824056E-4 36-37 2.125819835706014E-4 0.0 0.0 0.07698125080050403 8.503279342824056E-4 38-39 2.125819835706014E-4 0.0 0.0 0.12956871898628156 9.03473430175056E-4 40-41 2.125819835706014E-4 0.0 0.0 0.18577008089275932 9.566189260677063E-4 42-43 2.125819835706014E-4 0.0 0.0 0.24082881463754507 9.566189260677063E-4 44-45 2.125819835706014E-4 0.0 0.0 0.31036969601307807 9.831916740140314E-4 46-47 2.125819835706014E-4 0.0 0.0 0.3931172331179347 0.0010097644219603566 48-49 2.125819835706014E-4 0.0 0.0 0.47408439611038744 0.0010097644219603566 50-51 2.125819835706014E-4 0.0 0.0 0.5639534296648592 0.001062909917853007 52-53 2.125819835706014E-4 0.0 0.0 0.6509260336931815 0.0010894826657993322 54-55 2.125819835706014E-4 0.0 0.0 0.7432397600587151 0.0011160554137456574 56-57 2.125819835706014E-4 0.0 0.0 0.8531977910606088 0.0011160554137456574 58-59 2.125819835706014E-4 0.0 0.0 0.9699053000408688 0.0011160554137456574 60-61 2.125819835706014E-4 0.0 0.0 1.0949566518762752 0.0011692009096383076 62-63 2.125819835706014E-4 0.0 0.0 1.2142682901552753 0.0012754919014236084 64-65 2.125819835706014E-4 0.0 0.0 1.3391070600071109 0.001355210145262584 66-67 2.125819835706014E-4 0.0 0.0 1.4824404624295888 0.0014083556411552344 68-69 2.125819835706014E-4 0.0 0.0 1.6481215458749263 0.0014349283891015594 70-71 2.125819835706014E-4 0.0 0.0 1.8202598070712208 0.0014349283891015594 72-73 2.125819835706014E-4 0.0 0.0 1.9913883038455549 0.001514646632940535 74-75 2.125819835706014E-4 0.0 0.0 2.1655460938857702 0.001647510372672161 76-77 2.125819835706014E-4 0.0 0.0 2.34972180990175 0.0017006558685648111 78-79 2.125819835706014E-4 0.0 0.0 2.5547039875597024 0.0017272286165111363 80-81 3.7201847124855244E-4 0.0 0.0 2.7740354491086703 0.001806946860350112 82-83 3.7201847124855244E-4 0.0 0.0 2.9941640930960283 0.002285256323383965 84-85 3.9859121919487764E-4 0.0 0.0 3.215940247456058 0.002285256323383965 86-87 4.251639671412028E-4 0.0 0.0 3.4547761059976287 0.00236497456722294 88-89 4.251639671412028E-4 0.0 0.0 3.7074297934712885 0.002471265559008241 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3985 0.0 52.038364 1 GTATCAA 6600 0.0 40.57233 1 TATCAAC 8300 0.0 32.147728 2 ATCAACG 8335 0.0 31.778557 3 TCAACGC 8465 0.0 31.289692 4 CAACGCA 8530 0.0 30.994686 5 AACGCAG 8860 0.0 29.804575 6 ACGCAGA 10140 0.0 25.948584 7 CGCAGAG 10270 0.0 25.481382 8 GTGGTAT 1835 0.0 24.105291 1 GCAGAGT 11310 0.0 22.928303 9 TGGTATC 1785 0.0 22.91531 2 GAGTACT 7295 0.0 22.135813 12-13 GTGTAAG 680 0.0 21.682947 1 GTACATG 6345 0.0 21.663654 1 GTACTTT 7485 0.0 21.542189 14-15 TACATGG 6250 0.0 21.00364 2 TAAGGTG 1190 0.0 20.778675 5 CAGAGTA 11010 0.0 20.576567 10-11 AGAGTAC 10135 0.0 20.127102 10-11 >>END_MODULE