FastQCFastQC Report
Thu 26 May 2016
SRR938258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868630
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78190.41843489615386675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65820.35223666536446485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39750.21272268988510298No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25510.13651712752123213No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22230.11896416090932929No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22160.11858955491456308No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20060.10735137507157651No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA19410.10387289083446162No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA19110.10226743657117783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37800.051.524541
GTATCAA64950.040.7170521
TATCAAC83600.031.6066822
ATCAACG83750.031.3806823
TCAACGC84800.030.8239944
CAACGCA85850.030.280925
AACGCAG89500.029.1475166
GTGGTAT18700.026.2930741
TGGTATC17700.025.7755762
ACGCAGA100800.025.684557
CGCAGAG101150.025.5010628
TACATGG62550.024.7685572
GTAAGGT11450.024.488864
GTACATG64450.024.4420011
AGGTAAG10700.023.9839322
ACATGGG64350.023.3378373
TAAGGTG12250.023.2775485
ACCGTAT1452.3340774E-522.9326578
GTGTAAG7800.022.6439861
GCAGAGT111550.022.4841799
Produced by FastQC (version 0.11.5)