##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938258_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1868630 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.750237339655257 33.0 31.0 34.0 30.0 34.0 2 31.940086587499934 33.0 31.0 34.0 30.0 34.0 3 32.024556493259766 34.0 31.0 34.0 30.0 34.0 4 35.55108341405201 37.0 35.0 37.0 33.0 37.0 5 35.552925405243414 37.0 35.0 37.0 33.0 37.0 6 35.57441334025462 37.0 35.0 37.0 33.0 37.0 7 35.50992705886131 37.0 35.0 37.0 33.0 37.0 8 35.479800709610785 37.0 35.0 37.0 33.0 37.0 9 37.09725413805836 39.0 37.0 39.0 33.0 39.0 10-11 37.00000695696848 39.0 37.0 39.0 33.0 39.0 12-13 36.889882962384206 39.0 37.0 39.0 33.0 39.0 14-15 38.052406575940665 40.0 38.0 41.0 33.0 41.0 16-17 37.88589421126708 40.0 38.0 41.0 33.0 41.0 18-19 38.0212540738402 40.0 38.0 41.0 33.0 41.0 20-21 37.99162514783558 40.0 38.0 41.0 33.0 41.0 22-23 37.88964883363748 40.0 38.0 41.0 33.0 41.0 24-25 37.77536002311854 40.0 38.0 41.0 32.0 41.0 26-27 37.58557954223147 40.0 37.5 41.0 32.0 41.0 28-29 37.35917062232758 40.0 37.0 41.0 31.5 41.0 30-31 37.12796888629638 40.0 37.0 41.0 31.0 41.0 32-33 36.85894719660928 39.5 36.0 41.0 30.5 41.0 34-35 36.6115887040238 39.0 36.0 41.0 30.0 41.0 36-37 36.33818278631939 39.0 35.0 41.0 30.0 41.0 38-39 36.02498327651809 39.0 35.0 40.0 29.0 41.0 40-41 35.78506312111011 39.0 35.0 40.0 28.0 41.0 42-43 35.589385806714006 39.0 35.0 40.0 27.0 41.0 44-45 35.29657074969363 38.5 35.0 40.0 26.0 41.0 46-47 34.90117064373364 38.0 34.0 40.0 24.5 41.0 48-49 34.48658375387316 38.0 34.0 40.0 23.5 41.0 50-51 33.76907119119355 37.5 33.0 39.5 22.5 40.5 52-53 33.88592203914098 38.0 33.0 39.5 23.0 40.5 54-55 33.966486142253956 38.0 33.5 40.0 22.0 41.0 56-57 33.63243579520825 38.0 33.0 40.0 20.0 41.0 58-59 33.27534129281881 37.0 33.0 40.0 18.5 41.0 60-61 32.814858746782406 36.5 32.0 39.5 15.5 41.0 62-63 32.237938489695665 36.0 31.5 39.0 10.0 41.0 64-65 31.67549461370095 35.0 31.0 39.0 7.5 40.0 66-67 31.117490621471347 35.0 30.5 38.5 3.5 40.0 68-69 30.475449393405867 35.0 29.5 38.0 2.0 40.0 70-71 29.895599182288628 34.5 29.0 37.0 2.0 39.0 72-73 29.23760187945179 34.0 28.0 36.5 2.0 39.0 74-75 28.528741109797018 34.0 26.5 36.0 2.0 39.0 76-77 27.843674242626953 33.5 26.0 35.0 2.0 37.5 78-79 27.10841819942953 33.0 25.0 35.0 2.0 37.0 80-81 26.43646842874191 32.5 23.5 35.0 2.0 36.5 82-83 25.6900857847728 32.0 20.5 35.0 2.0 36.0 84-85 25.02261710450972 31.5 18.5 34.5 2.0 35.5 86-87 24.316488550435345 31.0 13.0 34.0 2.0 35.0 88-89 23.476759711660414 30.5 4.5 34.0 2.0 35.0 90-91 22.633184472046366 30.0 2.0 34.0 2.0 35.0 92-93 21.710564156628116 29.0 2.0 34.0 2.0 35.0 94-95 20.736415983902646 29.0 2.0 34.0 2.0 35.0 96-97 19.521899198878323 26.5 2.0 33.5 2.0 35.0 98-99 18.029774487191148 24.5 2.0 33.0 2.0 34.5 100-101 14.530920781535135 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3561147955346442 1101 2 0.26197564629627834 1101 3 0.19307875661203866 1101 4 0.1791481115206892 1101 5 0.22005428570513175 1101 6 0.2316347329138111 1101 7 0.2647687052119707 1101 8 0.22368223548195232 1101 9 0.29734188879672985 1101 10-11 0.26347633460675723 1101 12-13 0.22601293071578965 1101 14-15 0.47398204631091545 1101 16-17 0.515798055484538 1101 18-19 0.392495064913696 1101 20-21 0.40890026627081255 1101 22-23 0.4503849450027886 1101 24-25 0.4968544939517301 1101 26-27 0.5339091272050709 1101 28-29 0.5235976426208921 1101 30-31 0.7026112870642081 1101 32-33 0.6155711987917982 1101 34-35 0.6186856601050792 1101 36-37 0.5526562790312397 1101 38-39 0.5983589900215662 1101 40-41 0.9016812159578862 1101 42-43 0.909635084740259 1101 44-45 0.9893696928741775 1101 46-47 0.967087304816161 1101 48-49 1.0603060633677615 1101 50-51 1.1262284582296047 1101 52-53 1.215779704948119 1101 54-55 1.299128329433465 1101 56-57 1.2466309784749896 1101 58-59 1.3302535716966162 1101 60-61 1.185053362801277 1101 62-63 1.2164557832394465 1101 64-65 1.2875574099753528 1101 66-67 1.4187739056288464 1101 68-69 1.6025300043439366 1101 70-71 1.8965787085068087 1101 72-73 2.0214645662735506 1101 74-75 2.3355608055420873 1101 76-77 2.3572361393432466 1101 78-79 2.4209531411597 1101 80-81 2.4083809694572516 1101 82-83 2.64944039516811 1101 84-85 2.5714453298027173 1101 86-87 2.9721572710904915 1101 88-89 3.230676943337812 1101 90-91 3.6834431649556656 1101 92-93 3.913916860894684 1101 94-95 4.122978058587677 1101 96-97 4.244379561615059 1101 98-99 4.467524260470995 1101 100-101 4.0734761650187625 1102 1 0.3493941343166327 1102 2 0.1984108989575688 1102 3 0.13937693198978707 1102 4 0.054081971270150575 1102 5 0.04168076394434905 1102 6 0.16796740822698553 1102 7 8.852145830218205E-5 1102 8 0.14995126118164137 1102 9 0.38191322248674453 1102 10-11 0.2996916732411492 1102 12-13 0.29466097602693253 1102 14-15 0.3565457420473166 1102 16-17 0.39032289728297087 1102 18-19 0.1656365884456008 1102 20-21 0.3904800681267986 1102 22-23 0.35077145497455575 1102 24-25 0.22882523357868934 1102 26-27 0.18464838517972026 1102 28-29 0.23550952941682368 1102 30-31 0.2277973468763932 1102 32-33 0.3103784972808086 1102 34-35 0.39823310212858587 1102 36-37 0.36704441252939546 1102 38-39 0.283020540120269 1102 40-41 0.5196818801277701 1102 42-43 0.5141305923689927 1102 44-45 0.6114031596564544 1102 46-47 0.5241959519390136 1102 48-49 0.9262658810900319 1102 50-51 0.907835011372427 1102 52-53 0.7867859223161631 1102 54-55 0.8960871693500749 1102 56-57 0.9264488852329151 1102 58-59 0.9334541325511765 1102 60-61 0.9014301316148092 1102 62-63 0.929423515652779 1102 64-65 1.0447398537515262 1102 66-67 0.9334535359076099 1102 68-69 1.3271081642243878 1102 70-71 1.565041893201382 1102 72-73 1.5719274557815126 1102 74-75 1.724985929612707 1102 76-77 1.863855514654574 1102 78-79 2.1440920818087434 1102 80-81 2.3821008742595673 1102 82-83 2.648092499295185 1102 84-85 2.5411676698945946 1102 86-87 2.7130468897462876 1102 88-89 3.1070564795258484 1102 90-91 2.9539117737042595 1102 92-93 3.1397045479230776 1102 94-95 3.497153473232622 1102 96-97 3.9012155301038867 1102 98-99 3.9252302915024906 1102 100-101 3.712082699712413 1103 1 0.5728306155531584 1103 2 0.5337662228267135 1103 3 0.43332866668685455 1103 4 0.4072656689714549 1103 5 0.4526603392013513 1103 6 0.4042686653746088 1103 7 0.4860903337506457 1103 8 0.3602425819677961 1103 9 0.4648283051335298 1103 10-11 0.6822857510030715 1103 12-13 0.8125530925552482 1103 14-15 0.9187865269573763 1103 16-17 0.8013493421694875 1103 18-19 0.7639005373681229 1103 20-21 0.7820097886796518 1103 22-23 0.8544658233281908 1103 24-25 0.8947997573311781 1103 26-27 0.936122278796077 1103 28-29 1.0122890650950254 1103 30-31 1.012921759704092 1103 32-33 1.0551298278575132 1103 34-35 1.0309447705832397 1103 36-37 1.2082307349299484 1103 38-39 1.1414167655527763 1103 40-41 1.3090041452848808 1103 42-43 1.389929823707071 1103 44-45 1.4813247311430544 1103 46-47 1.4266183827021592 1103 48-49 1.3109359709755637 1103 50-51 1.1995095070031354 1103 52-53 1.3054816967586689 1103 54-55 1.5328837864043692 1103 56-57 1.5667657239284836 1103 58-59 1.5678881036586603 1103 60-61 1.7842900122534857 1103 62-63 1.7326973071974976 1103 64-65 1.7043078199220751 1103 66-67 1.8858427413111585 1103 68-69 2.0338217699570613 1103 70-71 2.0444034246925646 1103 72-73 2.3950375160871005 1103 74-75 2.422238119465952 1103 76-77 2.5156612452479443 1103 78-79 2.98280743734092 1103 80-81 3.074870661054831 1103 82-83 3.0708380180378327 1103 84-85 3.095548730398079 1103 86-87 3.259976095774764 1103 88-89 3.4324483617060153 1103 90-91 3.4844132787401385 1103 92-93 3.605516381983758 1103 94-95 3.930263637692942 1103 96-97 4.435151641591112 1103 98-99 4.77680479859022 1103 100-101 4.071925009447755 1104 1 0.3293985409330844 1104 2 0.21595902492151708 1104 3 0.19546173487530183 1104 4 0.1374466677440438 1104 5 0.28470794623954276 1104 6 0.34062321068000756 1104 7 0.38350299637731666 1104 8 0.36276341691473135 1104 9 0.38476022313141556 1104 10-11 0.3510460549683856 1104 12-13 0.34401850766889197 1104 14-15 0.5027589080380324 1104 16-17 0.6685173269933529 1104 18-19 0.5216092674235924 1104 20-21 0.4758752355374227 1104 22-23 0.4928978922251588 1104 24-25 0.539258632467245 1104 26-27 0.5991304853404884 1104 28-29 0.5721378149982073 1104 30-31 0.6386983367330075 1104 32-33 0.5888167597682639 1104 34-35 0.5764859603089789 1104 36-37 0.6475036798886009 1104 38-39 0.6972770868018472 1104 40-41 0.7483368012574942 1104 42-43 0.6614966597902239 1104 44-45 0.7079694261914753 1104 46-47 0.7048610246073821 1104 48-49 0.7967054818832153 1104 50-51 0.8625650245106726 1104 52-53 0.6381931834408263 1104 54-55 1.00105228363649 1104 56-57 1.006869472292152 1104 58-59 1.034800761897614 1104 60-61 1.1740844651956692 1104 62-63 1.1376774346852798 1104 64-65 1.2237434688091078 1104 66-67 1.4118115000508098 1104 68-69 1.4765915292472762 1104 70-71 1.640270506830884 1104 72-73 1.721332644164363 1104 74-75 1.8642753219076553 1104 76-77 2.1426087686144086 1104 78-79 2.218516569539581 1104 80-81 2.4117905342156654 1104 82-83 2.702888448472212 1104 84-85 2.856268002022908 1104 86-87 2.8111011237702606 1104 88-89 2.9362723466092397 1104 90-91 2.7661061870938433 1104 92-93 3.0613180223769483 1104 94-95 3.3263433902413446 1104 96-97 3.551898310842077 1104 98-99 3.6530177620230404 1104 100-101 3.8097476826163703 1105 1 0.19909949520119596 1105 2 0.2305520291596892 1105 3 0.09952006083798182 1105 4 0.11790873378586753 1105 5 0.02306950060660995 1105 6 0.2659323352119358 1105 7 0.13888868908668428 1105 8 0.19702679066166695 1105 9 0.23798201459531754 1105 10-11 0.20320107010134336 1105 12-13 0.19663834619552745 1105 14-15 0.25413286045097294 1105 16-17 0.3884583539091153 1105 18-19 0.32411777860710345 1105 20-21 0.3173605684330454 1105 22-23 0.2955451566605163 1105 24-25 0.18546928115936367 1105 26-27 0.2873928163715007 1105 28-29 0.38789194777857716 1105 30-31 0.38673822563594484 1105 32-33 0.4949355715208412 1105 34-35 0.32157793304957494 1105 36-37 0.4661156868274503 1105 38-39 0.6435031164395326 1105 40-41 0.6082370524304039 1105 42-43 0.7494054062047795 1105 44-45 0.7027143681926091 1105 46-47 0.6947144716514657 1105 48-49 0.7791207213595186 1105 50-51 0.7206895292796531 1105 52-53 0.6197942070265157 1105 54-55 0.7076673892011485 1105 56-57 0.7509850059253509 1105 58-59 0.7318863236475295 1105 60-61 0.959511270198341 1105 62-63 0.9336245407648036 1105 64-65 0.9835016602696989 1105 66-67 1.0328012358528156 1105 68-69 1.155335977641105 1105 70-71 1.3419923461203744 1105 72-73 1.4871766418167738 1105 74-75 1.4235991271240707 1105 76-77 1.5979097037522827 1105 78-79 1.7170797353337726 1105 80-81 1.8902073182813588 1105 82-83 1.9580654208644468 1105 84-85 2.0610930430289223 1105 86-87 2.1637631787118288 1105 88-89 2.493933917390521 1105 90-91 2.689829539088997 1105 92-93 2.6509198536836927 1105 94-95 3.2369947098142937 1105 96-97 3.2500240791513093 1105 98-99 3.5740035418812646 1105 100-101 3.546078863601389 1106 1 0.18243252508145957 1106 2 0.14118491087899798 1106 3 0.17277894196100618 1106 4 0.18720812134171183 1106 5 0.2300908735648406 1106 6 0.0413700472971783 1106 7 0.17271422329685038 1106 8 0.23903702309913655 1106 9 0.1505664788603056 1106 10-11 0.2233185860952176 1106 12-13 0.26243569568470093 1106 14-15 0.3547787603069139 1106 16-17 0.3492298378173899 1106 18-19 0.1979724329323389 1106 20-21 0.16991765364494427 1106 22-23 0.2550202321634316 1106 24-25 0.2573020276903222 1106 26-27 0.3267308600550862 1106 28-29 0.23537804773146576 1106 30-31 0.3276391284846838 1106 32-33 0.3537778335813968 1106 34-35 0.48809344215657546 1106 36-37 0.4392361933513058 1106 38-39 0.17553410506687328 1106 40-41 0.6522386956287676 1106 42-43 0.42436335898899813 1106 44-45 0.40438823428332427 1106 46-47 0.6019241182727768 1106 48-49 0.8400664252606376 1106 50-51 0.8600569889450469 1106 52-53 0.9858092461605779 1106 54-55 0.4667812227920365 1106 56-57 0.7796860712732112 1106 58-59 1.0345412155765104 1106 60-61 1.0478101388494423 1106 62-63 0.8446227041151033 1106 64-65 1.0381357300077632 1106 66-67 1.1914316118083974 1106 68-69 0.9861300725961222 1106 70-71 1.366965206036575 1106 72-73 1.6699781926136836 1106 74-75 1.751315923478991 1106 76-77 1.8133948569315983 1106 78-79 2.1041946249413392 1106 80-81 2.279590140258339 1106 82-83 2.2912779419449514 1106 84-85 2.5743965203734938 1106 86-87 2.2562488462440946 1106 88-89 2.3540108541228335 1106 90-91 2.5151614788141146 1106 92-93 2.789098725612085 1106 94-95 2.862475493269425 1106 96-97 2.7172232680212574 1106 98-99 2.8869466307755687 1106 100-101 3.0460785791141394 1107 1 0.22879711939125968 1107 2 0.21404386802704778 1107 3 0.15920952888217954 1107 4 0.0987398516986886 1107 5 0.1430850815048288 1107 6 0.17988748970773116 1107 7 0.16764907681390184 1107 8 0.20213906510711155 1107 9 0.16991227289912558 1107 10-11 0.10453012847651877 1107 12-13 0.1841758439653347 1107 14-15 0.2615058057615798 1107 16-17 0.3556298667966047 1107 18-19 0.27448123131642177 1107 20-21 0.2868753109991502 1107 22-23 0.36121002661099055 1107 24-25 0.35717762248076923 1107 26-27 0.44451207340866716 1107 28-29 0.5438891806731121 1107 30-31 0.5429052858883594 1107 32-33 0.5073333842109662 1107 34-35 0.5353553921663377 1107 36-37 0.633211677793625 1107 38-39 0.5862563365103028 1107 40-41 0.514186218364749 1107 42-43 0.6963716191488913 1107 44-45 0.6602886960585934 1107 46-47 0.8181081763480123 1107 48-49 0.8169268400203578 1107 50-51 0.6818054363128851 1107 52-53 0.6264817479596232 1107 54-55 0.7945727302576984 1107 56-57 0.8524031059201462 1107 58-59 0.7757224216852876 1107 60-61 1.0146437964869577 1107 62-63 1.1819093675630583 1107 64-65 1.2168092655432652 1107 66-67 1.342974836536328 1107 68-69 1.4101843686307802 1107 70-71 1.57065089284146 1107 72-73 1.7084870385755977 1107 74-75 1.9107232767405904 1107 76-77 2.125065319912533 1107 78-79 2.107538319067803 1107 80-81 2.2250135045406942 1107 82-83 2.334327767285661 1107 84-85 2.4950976607816138 1107 86-87 2.4848828867749972 1107 88-89 2.8067233580628717 1107 90-91 2.934058040829381 1107 92-93 3.0804830566110404 1107 94-95 3.5589074534597565 1107 96-97 3.674859266271689 1107 98-99 3.9501574259686905 1107 100-101 3.9957793524160596 1108 1 0.253549594638784 1108 2 0.12189988964768617 1108 3 0.11565888854364914 1108 4 0.11695178063914824 1108 5 0.20728886206759256 1108 6 0.16433064156525035 1108 7 0.1445897775540672 1108 8 0.10948428911130037 1108 9 0.1648258868875132 1108 10-11 0.17151522873103175 1108 12-13 0.24679338329751488 1108 14-15 0.42467046496737026 1108 16-17 0.46455002895135067 1108 18-19 0.40771431860667207 1108 20-21 0.3810364446599763 1108 22-23 0.4097192900565787 1108 24-25 0.3926031262933307 1108 26-27 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-1.202237530050418 2316 50-51 -1.389639437747995 2316 52-53 -1.418745091615932 2316 54-55 -1.3381736195251648 2316 56-57 -1.2487766943995666 2316 58-59 -1.236431639987181 2316 60-61 -1.2856305370209995 2316 62-63 -1.2773212630805233 2316 64-65 -1.302605468673125 2316 66-67 -1.3515477710427533 2316 68-69 -1.497009382891175 2316 70-71 -1.6798215304857855 2316 72-73 -1.6882062452405222 2316 74-75 -1.7089886371332454 2316 76-77 -1.8899545070408017 2316 78-79 -2.547783261265728 2316 80-81 -2.5005450467483534 2316 82-83 -2.3949580535448156 2316 84-85 -2.2793185156400817 2316 86-87 -1.9603294858189706 2316 88-89 -2.348656853981744 2316 90-91 -2.4652628871421207 2316 92-93 -2.9007169435686713 2316 94-95 -2.9898174079133106 2316 96-97 -3.244042069812469 2316 98-99 -3.264006580181123 2316 100-101 -2.9380378011109674 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5762.0 3 2006.0 4 2058.0 5 2309.0 6 2734.0 7 3351.0 8 4147.0 9 5206.0 10 6554.0 11 7898.0 12 9866.0 13 11492.0 14 13440.0 15 16062.0 16 19102.0 17 22642.0 18 22448.0 19 20054.0 20 20556.0 21 21517.0 22 23641.0 23 26102.0 24 29387.0 25 32714.0 26 36632.0 27 41600.0 28 47741.0 29 54923.0 30 65020.0 31 77167.0 32 93483.0 33 116319.0 34 150809.0 35 201175.0 36 257226.0 37 264971.0 38 123963.0 39 6552.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41310838751436 17.84216304091882 14.34545691089079 23.39927166067602 2 16.059684904137107 19.01394953412325 39.74011122794018 25.18625433379946 3 19.978882405949335 22.809464580108447 28.83135213106187 28.380300882880345 4 13.279298941515632 15.865539073108673 35.62296795418232 35.23219403119337 5 14.385433269919618 36.449710353574304 33.58179297993041 15.583063396575659 6 33.79483136487016 34.8181251432697 16.50499060579249 14.882052886067651 7 29.797282682023685 31.930842030192895 19.460798276404866 18.811077011378554 8 27.269480934919983 34.14678674125554 18.823624520100502 19.760107803723983 9 26.453223624223455 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28.598588378401963 23.3634457144601 34-35 23.176135136140843 25.804719292248045 28.37057979623781 22.648565775373296 36-37 23.24338982397492 25.37833239283393 28.246222029958208 23.132055753232944 38-39 22.7283457549056 25.752427171473368 28.211475594996255 23.307751478624777 40-41 23.218854782694343 25.589004534962896 28.373628055961188 22.81851262638158 42-43 22.923916412273194 25.633708727636524 28.152806303926464 23.289568556163818 44-45 22.862551078656516 25.984554568958895 28.233982463920114 22.918911888464475 46-47 23.34211145439075 26.17628740348104 27.51294569885897 22.96865544326924 48-49 23.092821638745598 26.199090141901355 27.44150654129464 23.266581678058408 50-51 23.202592077272037 26.550781094235777 27.372781315291345 22.873845513200845 52-53 23.514963435084226 26.155890770376182 27.369761406096703 22.959384388442885 54-55 23.094542975868148 26.371240998159557 27.57506362072228 22.959152405250016 56-57 23.098126795561257 26.495505995351444 27.39350406581305 23.01286314327425 58-59 23.08677340261375 26.064937546464822 27.361777697849814 23.486511353071617 60-61 22.810216829293097 26.35902349207029 27.648411113906302 23.182348564730315 62-63 22.930217907294594 26.376168211167993 27.752503687186366 22.94111019435105 64-65 22.659954295140775 26.470842478274935 27.554747211556656 23.314456015027634 66-67 22.616435632489342 26.443170837377465 27.531534887405122 23.408858642728063 68-69 23.150762794959515 25.992430924559727 27.73518619219093 23.121620088289824 70-71 22.902990381226303 26.23770020824039 27.327958138891333 23.531351271641974 72-73 22.941784600694263 26.337711885839166 27.26844533151993 23.45205818194664 74-75 22.905296443364715 26.653837281433468 27.160295595030316 23.280570680171508 76-77 23.14474810256048 26.33770757300613 27.133853446647176 23.38369087778622 78-79 23.22444316524121 26.491255254300516 27.139821998067283 23.144479582390993 80-81 23.38688569341073 26.46921073830533 26.90100173155307 23.24290183673087 82-83 23.277190473412336 26.605699531506495 26.93128458638709 23.18582540869408 84-85 23.289354631438748 26.63602135876446 26.863412504987643 23.21121150480915 86-87 23.548799091495102 26.592820317414784 26.54200590881396 23.31637468227615 88-89 23.37251610340654 26.70063375128407 26.4291790032884 23.49767114202099 90-91 23.421432358839965 26.69445172335248 26.420553625864247 23.46356229194331 92-93 23.580885011455578 26.288796226094956 26.612084171549217 23.518234590900246 94-95 23.35073859031337 26.131921641012024 26.782504237111205 23.7348355315634 96-97 23.765475137404366 25.749959518070998 26.712265154627584 23.77230018989705 98-99 23.4739627828701 26.018455474121225 26.28596296272506 24.221618780283613 100-101 24.57786176654386 24.96677131914647 26.29658848636927 24.15877842794039 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 608.0 1 433.0 2 391.0 3 533.0 4 416.5 5 377.5 6 706.5 7 1523.0 8 2537.5 9 2991.0 10 2460.5 11 1767.5 12 1651.0 13 1722.5 14 1884.0 15 2070.5 16 2221.0 17 2372.5 18 2534.5 19 2786.0 20 3166.5 21 3720.0 22 4466.0 23 5357.5 24 6520.5 25 8132.5 26 10214.0 27 12486.5 28 14545.0 29 16561.5 30 18677.5 31 21659.0 32 26732.0 33 29115.5 34 28840.5 35 30316.0 36 33178.0 37 36232.5 38 38865.0 39 41857.0 40 44970.5 41 48912.5 42 51623.0 43 53607.0 44 56580.0 45 60763.5 46 63789.0 47 66054.5 48 68043.5 49 67985.5 50 68083.5 51 67971.0 52 68455.0 53 70145.0 54 72115.5 55 70253.5 56 66011.0 57 62107.0 58 58672.5 59 52875.5 60 43472.5 61 36229.5 62 30608.5 63 23642.0 64 17439.0 65 13598.5 66 10488.5 67 8004.0 68 6155.5 69 4696.0 70 3582.5 71 2626.5 72 1840.0 73 1381.5 74 1068.5 75 791.5 76 572.0 77 359.5 78 236.5 79 170.5 80 111.0 81 65.5 82 39.5 83 26.5 84 17.5 85 11.5 86 10.0 87 8.5 88 7.0 89 5.5 90 5.0 91 3.5 92 4.0 93 3.5 94 1.0 95 1.0 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45530682906728454 2 0.04035041715053274 3 0.05314053611469366 4 0.056404959783370706 5 0.08166410685903577 6 0.08145004629059793 7 0.04848471875117064 8 0.04233047740858276 9 0.04832417332484226 10-11 0.051481566709300396 12-13 0.0470130523431605 14-15 0.040965841284791533 16-17 0.039922296013657065 18-19 0.047146840198434146 20-21 0.05498680851747002 22-23 0.04736090076687199 24-25 0.06403086753396874 26-27 0.05589656593333084 28-29 0.04417674981135913 30-31 0.04615681006940914 32-33 0.045434355650931436 34-35 0.0488860823169916 36-37 0.044310537666632774 38-39 0.027239207333715074 40-41 0.025580237928321817 42-43 0.024670480512461 44-45 0.016616451624987292 46-47 0.02881790402594414 48-49 0.02871087374172522 50-51 0.01720511818819135 52-53 0.0165361789118231 54-55 0.017740269609285948 56-57 0.01645590619865891 58-59 0.015037754932758223 60-61 0.018195148317216355 62-63 0.017954330177723788 64-65 0.01811487560405217 66-67 0.009820028577085888 68-69 0.01273660382205145 70-71 0.019078148162022444 72-73 0.01704457276186297 74-75 0.014074482374787946 76-77 0.010221392142906837 78-79 0.014957482219594035 80-81 0.019533026869952855 82-83 0.01610805777494742 84-85 0.014101239945842677 86-87 0.003505241808169622 88-89 0.02927278273387455 90-91 0.032082327694621195 92-93 0.017472693898738647 94-95 0.03275126697098944 96-97 0.027426510331098185 98-99 0.02450993508613262 100-101 0.14834397392742277 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1868630.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.69973698739272 #Duplication Level Percentage of deduplicated Percentage of total 1 80.60844548248609 48.12302994267004 2 11.459377835870775 13.682436856812854 3 3.372107764940343 6.0394183998024955 4 1.4764363859176868 3.5257145567161015 5 0.7893405634418214 2.356171201547855 6 0.4846226601361118 1.735910720895389 7 0.32066252714432986 1.3400427972560418 8 0.22541146573977136 1.0765604174885628 9 0.18094342812350633 0.9722047561713474 >10 0.9780840223460726 10.59643877502896 >50 0.06510404926953259 2.6562746684359815 >100 0.033878656700553705 3.7162748422417056 >500 0.0035132444750227217 1.5224418412705227 >1k 0.0018917470250122345 1.882602673902855 >5k 1.8016638333449853E-4 0.7744775497593274 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7819 0.41843489615386675 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6582 0.35223666536446485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3975 0.21272268988510298 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2551 0.13651712752123213 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2223 0.11896416090932929 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2216 0.11858955491456308 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2006 0.10735137507157651 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1941 0.10387289083446162 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1911 0.10226743657117783 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.351514210945987E-5 2 0.0 0.0 0.0 0.0 5.351514210945987E-5 3 0.0 0.0 0.0 0.0 5.351514210945987E-5 4 0.0 0.0 0.0 0.0 5.351514210945987E-5 5 0.0 0.0 0.0 0.0 5.351514210945987E-5 6 0.0 0.0 0.0 0.0 1.605454263283796E-4 7 0.0 0.0 0.0 0.0 1.605454263283796E-4 8 0.0 0.0 0.0 0.0 1.605454263283796E-4 9 0.0 0.0 0.0 0.0 1.605454263283796E-4 10-11 5.351514210945987E-5 0.0 0.0 0.0 1.605454263283796E-4 12-13 5.351514210945987E-5 0.0 0.0 0.0 4.8163627898513883E-4 14-15 5.351514210945987E-5 0.0 0.0 0.0 6.956968474229783E-4 16-17 5.351514210945987E-5 0.0 0.0 5.351514210945987E-5 8.56242273751358E-4 18-19 5.351514210945987E-5 0.0 0.0 5.351514210945987E-5 8.56242273751358E-4 20-21 5.351514210945987E-5 0.0 0.0 1.0703028421891974E-4 0.0010167877000797376 22-23 5.351514210945987E-5 0.0 0.0 1.8730299738310956E-4 0.0010167877000797376 24-25 5.351514210945987E-5 5.351514210945987E-5 0.0 2.4081813949256942E-4 0.0010167877000797376 26-27 5.351514210945987E-5 5.351514210945987E-5 0.0 7.224544184777082E-4 0.0010970604132439273 28-29 8.02727131641898E-5 5.351514210945987E-5 0.0 0.002836302531801373 0.0011238179842986574 30-31 1.0703028421891974E-4 5.351514210945987E-5 0.0 0.009605968008648047 0.0011505755553533872 32-33 1.0703028421891974E-4 5.351514210945987E-5 0.0 0.02175390526749544 0.001177333126408117 34-35 1.0703028421891974E-4 5.351514210945987E-5 0.0 0.04192911384276181 0.001230848268517577 36-37 1.0703028421891974E-4 5.351514210945987E-5 0.0 0.07735613791922424 0.001230848268517577 38-39 1.0703028421891974E-4 5.351514210945987E-5 0.0 0.1304966740339179 0.001230848268517577 40-41 1.0703028421891974E-4 5.351514210945987E-5 0.0 0.1854834825513879 0.001230848268517577 42-43 1.0703028421891974E-4 5.351514210945987E-5 0.0 0.23939998822666875 0.001230848268517577 44-45 1.3378785527364968E-4 5.351514210945987E-5 0.0 0.309424551676897 0.001230848268517577 46-47 2.140605684378395E-4 5.351514210945987E-5 0.0 0.3908210828253854 0.0012576058395723068 48-49 2.140605684378395E-4 5.351514210945987E-5 0.0 0.47591015877942666 0.0014449088369554166 50-51 2.140605684378395E-4 5.351514210945987E-5 0.0 0.5687321727682848 0.0014449088369554166 52-53 2.140605684378395E-4 5.351514210945987E-5 0.0 0.6551323697040077 0.0015519391211743362 54-55 2.6757571054729937E-4 5.351514210945987E-5 0.0 0.7470981414191145 0.001605454263283796 56-57 2.6757571054729937E-4 5.351514210945987E-5 0.0 0.8577139401593681 0.001658969405393256 58-59 2.6757571054729937E-4 5.351514210945987E-5 0.0 0.9681424359022386 0.001658969405393256 60-61 2.6757571054729937E-4 5.351514210945987E-5 0.0 1.0939030198594693 0.001658969405393256 62-63 2.6757571054729937E-4 5.351514210945987E-5 0.0 1.2160780892953662 0.0018195148317216357 64-65 2.6757571054729937E-4 5.351514210945987E-5 0.0 1.340447279557751 0.0018730299738310954 66-67 2.6757571054729937E-4 5.351514210945987E-5 0.0 1.4869449810823974 0.0018730299738310954 68-69 2.6757571054729937E-4 5.351514210945987E-5 0.0 1.6493366798135534 0.0018730299738310954 70-71 2.9433328160202927E-4 8.02727131641898E-5 0.0 1.816491226192451 0.0019533026869952854 72-73 3.210908526567592E-4 1.0703028421891974E-4 0.0 1.9915660136035491 0.002113848113323665 74-75 3.210908526567592E-4 1.0703028421891974E-4 0.0 2.1560180453059195 0.0022208783975425847 76-77 3.210908526567592E-4 1.0703028421891974E-4 0.0 2.3375681649122617 0.0022476359685973148 78-79 3.210908526567592E-4 1.0703028421891974E-4 0.0 2.541005977641374 0.002274393539652045 80-81 4.013635658209491E-4 1.0703028421891974E-4 0.0 2.758357727318945 0.002354666252816234 82-83 4.28121136875679E-4 1.0703028421891974E-4 0.0 2.9773952039729643 0.002461696537035154 84-85 4.28121136875679E-4 1.0703028421891974E-4 0.0 3.1959242867769433 0.002461696537035154 86-87 4.28121136875679E-4 1.0703028421891974E-4 0.0 3.4248888222922673 0.002595484392308804 88-89 4.28121136875679E-4 1.0703028421891974E-4 0.0 3.667606749329723 0.0027292722475824533 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3780 0.0 51.52454 1 GTATCAA 6495 0.0 40.717052 1 TATCAAC 8360 0.0 31.606682 2 ATCAACG 8375 0.0 31.380682 3 TCAACGC 8480 0.0 30.823994 4 CAACGCA 8585 0.0 30.28092 5 AACGCAG 8950 0.0 29.147516 6 GTGGTAT 1870 0.0 26.293074 1 TGGTATC 1770 0.0 25.775576 2 ACGCAGA 10080 0.0 25.68455 7 CGCAGAG 10115 0.0 25.501062 8 TACATGG 6255 0.0 24.768557 2 GTAAGGT 1145 0.0 24.48886 4 GTACATG 6445 0.0 24.442001 1 AGGTAAG 1070 0.0 23.983932 2 ACATGGG 6435 0.0 23.337837 3 TAAGGTG 1225 0.0 23.277548 5 ACCGTAT 145 2.3340774E-5 22.932657 8 GTGTAAG 780 0.0 22.643986 1 GCAGAGT 11155 0.0 22.484179 9 >>END_MODULE