FastQCFastQC Report
Thu 26 May 2016
SRR938258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868630
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77910.41693647217480184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67290.3601033912545555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41450.22182026404371116No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26130.13983506633201864No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22680.12137234230425499No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA21450.11478997982479143No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20670.11061579874025355No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA20610.1102947078875968No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.10199986086063051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37600.050.408541
GTATCAA63000.039.9626581
TATCAAC81850.030.2949942
ATCAACG82600.029.90333
TCAACGC83000.029.7567984
CAACGCA84300.029.0725465
AACGCAG87300.028.073496
GTGGTAT18750.027.3616031
TGGTATC18100.026.5070762
ACGCAGA100050.024.2585457
CGCAGAG101300.023.8185448
TAAGGTG11550.023.0285725
TAGGACG1452.3367127E-522.929314
GTAAGGT11050.022.7810924
TACATGG62000.021.8359572
GTACATG63100.021.6811451
GCAGAGT111600.021.492569
GAGTACT71650.020.81490312-13
ACATGGG62800.020.3464413
GTACTTT73700.020.2681514-15
Produced by FastQC (version 0.11.5)