##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938258_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1868630 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47082729058187 33.0 31.0 34.0 31.0 34.0 2 32.707390976276734 34.0 31.0 34.0 31.0 34.0 3 32.66926090237233 34.0 31.0 34.0 31.0 34.0 4 36.19837528028555 37.0 35.0 37.0 35.0 37.0 5 36.15476793158624 37.0 35.0 37.0 35.0 37.0 6 36.21824170649085 37.0 37.0 37.0 35.0 37.0 7 36.17774786875947 37.0 37.0 37.0 35.0 37.0 8 36.17032852945741 37.0 37.0 37.0 35.0 37.0 9 37.8763661077902 39.0 38.0 39.0 35.0 39.0 10-11 37.84048259955155 39.0 38.0 39.0 35.0 39.0 12-13 37.77741955336262 39.0 38.0 39.0 35.0 39.0 14-15 39.04695338295971 40.0 38.0 41.0 36.0 41.0 16-17 38.975308648582114 40.0 38.0 41.0 36.0 41.0 18-19 38.96300899589539 40.0 38.0 41.0 35.5 41.0 20-21 38.9623066096552 40.0 38.5 41.0 35.0 41.0 22-23 38.992096883813275 40.0 38.5 41.0 35.0 41.0 24-25 38.93467219299701 40.0 38.0 41.0 35.0 41.0 26-27 38.76144769162434 40.0 38.0 41.0 35.0 41.0 28-29 38.677767936937755 40.0 38.0 41.0 34.5 41.0 30-31 38.45099859255176 40.0 38.0 41.0 34.0 41.0 32-33 38.24766700737973 40.0 38.0 41.0 34.0 41.0 34-35 38.04091901553545 40.0 37.5 41.0 33.5 41.0 36-37 37.95714373632019 40.0 37.5 41.0 33.0 41.0 38-39 37.90441821013256 40.0 37.0 41.0 33.0 41.0 40-41 37.77196743068451 40.0 37.0 41.0 33.0 41.0 42-43 37.56539496850634 40.0 37.0 41.0 33.0 41.0 44-45 37.302434136238844 40.0 37.0 41.0 32.0 41.0 46-47 36.94286830458678 40.0 36.0 41.0 31.0 41.0 48-49 36.55509704971021 39.0 36.0 41.0 30.5 41.0 50-51 36.23619790969855 39.0 35.0 41.0 30.0 41.0 52-53 35.73668008112895 39.0 35.0 40.0 28.5 41.0 54-55 35.39232673134863 38.0 35.0 40.0 28.0 41.0 56-57 35.09247710889797 38.0 34.0 40.0 27.0 41.0 58-59 34.675381161599674 38.0 34.0 40.0 26.0 41.0 60-61 34.225361093421384 37.0 34.0 40.0 26.0 41.0 62-63 33.757920776183624 36.5 33.0 39.5 24.5 41.0 64-65 33.29203266564274 36.0 32.5 39.0 23.5 40.5 66-67 32.75467856129892 35.0 32.0 39.0 22.0 40.0 68-69 32.19396295681863 35.0 31.0 38.0 20.5 40.0 70-71 31.57757314182048 35.0 31.0 37.0 18.5 39.5 72-73 31.0972220289731 34.5 30.0 37.0 17.5 39.0 74-75 30.539535113960497 34.0 30.0 36.0 12.5 39.0 76-77 29.371398029572468 32.5 28.5 35.0 12.0 37.0 78-79 30.075332730396067 34.0 30.0 35.0 9.5 37.0 80-81 29.877881121463318 34.0 30.0 35.0 7.0 37.0 82-83 29.409805847064426 34.0 29.0 35.0 4.0 36.5 84-85 28.89191439717868 34.0 29.0 35.0 2.0 36.0 86-87 28.333419938671646 33.5 28.0 35.0 2.0 35.5 88-89 27.684436993947436 33.0 26.5 35.0 2.0 35.0 90-91 27.123204968345796 33.0 25.5 35.0 2.0 35.0 92-93 26.458972616301782 32.0 25.0 34.5 2.0 35.0 94-95 25.672747146305046 32.0 23.0 34.0 2.0 35.0 96-97 24.80652617158025 31.5 18.5 34.0 2.0 35.0 98-99 23.728724252527254 31.0 2.0 34.0 2.0 35.0 100-101 20.2450276405709 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.02043039396251345 1101 2 0.07253195470521945 1101 3 0.14454485578072962 1101 4 0.037825822053640934 1101 5 -0.01433454218066288 1101 6 0.0091501762428976 1101 7 0.02951713659977173 1101 8 0.07828789968226602 1101 9 0.07262581429468185 1101 10-11 0.1510140365916044 1101 12-13 0.025050359610929718 1101 14-15 0.15890430876115147 1101 16-17 0.15389288399584444 1101 18-19 0.26615659694758875 1101 20-21 0.19152642426947608 1101 22-23 0.13105924544131398 1101 24-25 0.13322002285045897 1101 26-27 0.09881563921288006 1101 28-29 0.2363667816630297 1101 30-31 0.2514382768515091 1101 32-33 0.3129877974521662 1101 34-35 0.22205786090130886 1101 36-37 0.28171090278732436 1101 38-39 0.41978461909516795 1101 40-41 0.4577023605842854 1101 42-43 0.5199286837100701 1101 44-45 0.4683763248314179 1101 46-47 0.44809555467045215 1101 48-49 0.4621859782736095 1101 50-51 0.452016219834249 1101 52-53 0.4359573192517985 1101 54-55 0.5863473728700868 1101 56-57 0.6617060449977217 1101 58-59 0.7500170449981738 1101 60-61 0.721415822359333 1101 62-63 0.8142332380291535 1101 64-65 0.7538650522178827 1101 66-67 0.9831730750423375 1101 68-69 0.8836328710359993 1101 70-71 0.9553064173042678 1101 72-73 0.9671866590798146 1101 74-75 1.0123027927845207 1101 76-77 1.147045334172411 1101 78-79 1.2129990183356654 1101 80-81 1.2816723404159127 1101 82-83 1.3185072030347378 1101 84-85 1.5576559869639972 1101 86-87 1.5813520570879867 1101 88-89 1.6123693695549726 1101 90-91 1.9243442766482453 1101 92-93 2.1690705697971 1101 94-95 2.141852381459291 1101 96-97 2.409460645331695 1101 98-99 2.286610158258089 1101 100-101 2.76219611998307 1102 1 0.05520854277347098 1102 2 0.0645050927234152 1102 3 0.15768213600507153 1102 4 0.0688580527419802 1102 5 -0.07918888603617091 1102 6 -0.06472396356486598 1102 7 0.039940175914935594 1102 8 0.04666271288449053 1102 9 0.015212018010345218 1102 10-11 0.07057236362146568 1102 12-13 0.07512489423078961 1102 14-15 0.14750881404684435 1102 16-17 0.07741778114176157 1102 18-19 0.0825344727109183 1102 20-21 0.08098752420858801 1102 22-23 0.1692384975122323 1102 24-25 -0.04761387933208283 1102 26-27 0.0651472075762598 1102 28-29 -0.11918807692413225 1102 30-31 -0.08855489695805119 1102 32-33 0.04203618960757893 1102 34-35 -0.03644591195259039 1102 36-37 0.02330011371660845 1102 38-39 0.1691335481212306 1102 40-41 0.18956701752209426 1102 42-43 0.216378326718754 1102 44-45 0.2776127139611688 1102 46-47 0.2634471034777164 1102 48-49 0.2293389033884523 1102 50-51 0.20726389985195937 1102 52-53 0.31114836033808757 1102 54-55 0.2877598408146653 1102 56-57 0.34639121002548023 1102 58-59 0.2915418683474371 1102 60-61 0.4294172244957508 1102 62-63 0.29092880838503987 1102 64-65 0.24941345359787448 1102 66-67 0.4047242806951701 1102 68-69 0.422031299167287 1102 70-71 0.6463334822268756 1102 72-73 0.5799368758574843 1102 74-75 0.5747759031289696 1102 76-77 0.6192372792677006 1102 78-79 0.6360711730134518 1102 80-81 0.8025099324311142 1102 82-83 0.6616787987028694 1102 84-85 0.7647757035848493 1102 86-87 0.7943504151124507 1102 88-89 1.232183033005338 1102 90-91 1.4287824073790212 1102 92-93 1.5076025698708975 1102 94-95 1.4319770978034043 1102 96-97 1.7055990509549979 1102 98-99 1.9138261795115277 1102 100-101 2.215712346022219 1103 1 0.34583190884610815 1103 2 0.32270425740245656 1103 3 0.33891178677425415 1103 4 0.19170356002759803 1103 5 0.10011973485102033 1103 6 0.15680690269004316 1103 7 0.2254030916225105 1103 8 0.18337788093815277 1103 9 0.20664841592308392 1103 10-11 0.37948655053754976 1103 12-13 0.2534713337012633 1103 14-15 0.47221681625491385 1103 16-17 0.413766324537832 1103 18-19 0.4149905454525822 1103 20-21 0.37772935587371137 1103 22-23 0.4894865940844966 1103 24-25 0.5239339641441845 1103 26-27 0.5109311281686928 1103 28-29 0.47271993361632525 1103 30-31 0.4109170385493357 1103 32-33 0.45515218673291 1103 34-35 0.461701800288715 1103 36-37 0.39338979094109305 1103 38-39 0.4478878575232841 1103 40-41 0.5441293072597233 1103 42-43 0.5526739595108268 1103 44-45 0.5950505075473203 1103 46-47 0.5814367088354402 1103 48-49 0.6898030168754445 1103 50-51 0.8081348417232022 1103 52-53 0.7510327485852741 1103 54-55 0.8655225971655014 1103 56-57 0.9973126669418733 1103 58-59 1.0627947456219147 1103 60-61 1.031482612836669 1103 62-63 1.062460612032531 1103 64-65 1.099966827690423 1103 66-67 0.9928487664311731 1103 68-69 1.0647678606029558 1103 70-71 1.0420706373445192 1103 72-73 0.9722065116283858 1103 74-75 0.9746336277646854 1103 76-77 0.5491709327032446 1103 78-79 0.9838088280738084 1103 80-81 1.1553755415800673 1103 82-83 1.1531468596542176 1103 84-85 1.1679053449801025 1103 86-87 1.2614812647026028 1103 88-89 1.444579320930675 1103 90-91 1.4987096032211618 1103 92-93 1.5073119781678628 1103 94-95 1.6326409800668955 1103 96-97 1.9578453560658744 1103 98-99 2.441761728563197 1103 100-101 2.6283236093149647 1104 1 0.12736705039936425 1104 2 0.0975586331133158 1104 3 0.18502372222378227 1104 4 0.1084095571118695 1104 5 0.07481256651518464 1104 6 0.04804624770791577 1104 7 -0.02867051584224356 1104 8 4.1837416257095583E-4 1104 9 0.015644519254806255 1104 10-11 0.14049541104155594 1104 12-13 0.06491101012479561 1104 14-15 0.11929439135440134 1104 16-17 0.174494562353253 1104 18-19 0.1762584716337443 1104 20-21 0.2209240362900644 1104 22-23 0.1311695747667443 1104 24-25 0.1257696676677469 1104 26-27 0.18408162243471793 1104 28-29 0.22494288118853234 1104 30-31 0.15508529464815268 1104 32-33 0.1867269512177856 1104 34-35 0.14540111317327842 1104 36-37 0.2759284972228073 1104 38-39 0.24258727456827955 1104 40-41 0.34545874532376075 1104 42-43 0.17254506002868908 1104 44-45 0.10269256348338018 1104 46-47 0.24076934954644003 1104 48-49 0.2930639631633909 1104 50-51 0.43519091322685455 1104 52-53 0.4306135276671057 1104 54-55 0.5659278308263325 1104 56-57 0.5295070142737828 1104 58-59 0.5020425089388993 1104 60-61 0.6492682155764342 1104 62-63 0.6812695051200635 1104 64-65 0.5212261234362146 1104 66-67 0.6506621147615803 1104 68-69 0.8558107888158162 1104 70-71 0.6837290086951349 1104 72-73 0.690511123264514 1104 74-75 0.6969503235364947 1104 76-77 0.7212968012314178 1104 78-79 0.9566061688924492 1104 80-81 0.9576819128787939 1104 82-83 0.9311794637755391 1104 84-85 1.0773512076936065 1104 86-87 1.4096250534026815 1104 88-89 1.532742346878834 1104 90-91 1.411438617852074 1104 92-93 1.8093031974057219 1104 94-95 1.7747198085253544 1104 96-97 2.1422617734279292 1104 98-99 2.486756171432731 1104 100-101 2.9304643922427296 1105 1 0.0593006094379831 1105 2 -0.0022864355961118576 1105 3 0.056571275620171946 1105 4 -0.013232466583637859 1105 5 0.023228830994234784 1105 6 -0.06342161999115348 1105 7 0.03843409938613718 1105 8 -0.01105592932562871 1105 9 0.0210709522806809 1105 10-11 0.029503732694031726 1105 12-13 0.018350289930474162 1105 14-15 0.038101154719285546 1105 16-17 0.1369987101898218 1105 18-19 0.08591814828552913 1105 20-21 3.133035193840783E-4 1105 22-23 -0.012054336374497154 1105 24-25 -0.014243581791710369 1105 26-27 0.04839275091786277 1105 28-29 0.024810504681873624 1105 30-31 0.18001881117513818 1105 32-33 0.053600291345112794 1105 34-35 0.08698289433991846 1105 36-37 0.05743360393291397 1105 38-39 -0.012665716892790613 1105 40-41 -0.13975650525280514 1105 42-43 0.10984984601213199 1105 44-45 0.046776183868651344 1105 46-47 -0.012809303472408828 1105 48-49 0.022747239676775166 1105 50-51 0.05455428378542848 1105 52-53 0.1746525994019521 1105 54-55 0.4135054647468408 1105 56-57 0.27411984546598944 1105 58-59 0.3509809579963843 1105 60-61 0.15705817739862482 1105 62-63 0.22388358095845717 1105 64-65 0.22481817942875892 1105 66-67 0.31434179786095484 1105 68-69 0.3664183273095176 1105 70-71 0.621376839952287 1105 72-73 0.5243600085740283 1105 74-75 0.6079635824429808 1105 76-77 0.5887123123098341 1105 78-79 0.8432103690419481 1105 80-81 0.9468687538746536 1105 82-83 1.0042770386048723 1105 84-85 1.1894535024952653 1105 86-87 1.350427749821197 1105 88-89 1.4737469735617346 1105 90-91 1.3563904793302761 1105 92-93 1.4963754978874029 1105 94-95 1.6512105203236302 1105 96-97 1.935733314840757 1105 98-99 2.0790471588481054 1105 100-101 2.14006343077126 1106 1 0.10251080910251886 1106 2 0.06239572386695613 1106 3 0.15094480055377346 1106 4 0.08214871219497866 1106 5 0.08977608887850863 1106 6 0.019955903695255017 1106 7 0.03025110808233933 1106 8 0.02320487037941632 1106 9 0.04944286587848978 1106 10-11 0.1256535960263534 1106 12-13 0.08772904942950532 1106 14-15 0.11423136567226777 1106 16-17 0.14323694664197006 1106 18-19 0.04141609201167995 1106 20-21 -0.027344262589771517 1106 22-23 0.05385026480851707 1106 24-25 0.06785943900772651 1106 26-27 0.0819281081314358 1106 28-29 -0.003684876383111657 1106 30-31 0.20647128928668934 1106 32-33 0.106035843603145 1106 34-35 0.08319267068741709 1106 36-37 0.25882753456809127 1106 38-39 0.24248947659268083 1106 40-41 0.19458628367813446 1106 42-43 0.3003982691561973 1106 44-45 0.35286569038569127 1106 46-47 0.11727988897069963 1106 48-49 0.27257939219835237 1106 50-51 0.3217467018587854 1106 52-53 0.2998870168972303 1106 54-55 0.37527521442081735 1106 56-57 0.48212497534010623 1106 58-59 0.42891396466248466 1106 60-61 0.45526454217500145 1106 62-63 0.4427362097344556 1106 64-65 0.5136694654249325 1106 66-67 0.4835937898406897 1106 68-69 0.23433317230947637 1106 70-71 0.30758114276506987 1106 72-73 0.5086693987016417 1106 74-75 0.6561651109360831 1106 76-77 0.6416659158529505 1106 78-79 1.0000587168537862 1106 80-81 1.1020033504831126 1106 82-83 1.1043249462580356 1106 84-85 1.0393324247209996 1106 86-87 1.3720063923390704 1106 88-89 1.5644713805196098 1106 90-91 1.5814346886491712 1106 92-93 1.5681934510812816 1106 94-95 1.8836673294691018 1106 96-97 1.9009698261622106 1106 98-99 2.4413234665222063 1106 100-101 2.622525645174509 1107 1 0.12205583530005981 1107 2 0.1002898426525789 1107 3 0.1538310987337539 1107 4 0.11149423049864993 1107 5 0.15594738744506742 1107 6 0.08930221135836547 1107 7 -0.02529465557154964 1107 8 0.024442975246358856 1107 9 0.05602131171626468 1107 10-11 0.08672668692541663 1107 12-13 0.12291947698658134 1107 14-15 0.16814028981320917 1107 16-17 0.1570145127224407 1107 18-19 0.16913701668678272 1107 20-21 0.11607585883101734 1107 22-23 0.14529985312513105 1107 24-25 0.21688563438716102 1107 26-27 0.1341453160589836 1107 28-29 0.17988255248398133 1107 30-31 0.24013949664600887 1107 32-33 0.29470721110349274 1107 34-35 0.23007407916416867 1107 36-37 0.1368871809458838 1107 38-39 0.19874260902502527 1107 40-41 0.3555812766523019 1107 42-43 0.317806077651106 1107 44-45 0.4062516639010525 1107 46-47 0.44105444899741997 1107 48-49 0.5696118332491551 1107 50-51 0.567484296075051 1107 52-53 0.5095334487492948 1107 54-55 0.6637294540108698 1107 56-57 0.5938266342384324 1107 58-59 0.7660927670095958 1107 60-61 0.8686949252684428 1107 62-63 0.9574100502005862 1107 64-65 0.860392218061854 1107 66-67 0.8533451578384401 1107 68-69 0.8747038522561255 1107 70-71 0.942944526543517 1107 72-73 1.0454997745502723 1107 74-75 1.0310812414961354 1107 76-77 1.017199341710743 1107 78-79 1.29530644866189 1107 80-81 1.2360383396510422 1107 82-83 1.435489478500397 1107 84-85 1.648092368081759 1107 86-87 1.6237815049173996 1107 88-89 1.5750464991533057 1107 90-91 1.7233065031648387 1107 92-93 1.9046129004652315 1107 94-95 2.1623387834745884 1107 96-97 2.3149950672817816 1107 98-99 2.5467367421421905 1107 100-101 2.9914385891013318 1108 1 0.06758921367637782 1108 2 0.04257809405339685 1108 3 0.20224841352834488 1108 4 0.03491243429953528 1108 5 0.03424394978473089 1108 6 0.04731977812274124 1108 7 0.042660731552587094 1108 8 0.033934768448254715 1108 9 0.0894177221628496 1108 10-11 0.07640138682229747 1108 12-13 -0.00704371815283622 1108 14-15 0.1322701558526589 1108 16-17 0.06620823481399185 1108 18-19 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-0.5975472873740273 2316 50-51 -0.5340804292904195 2316 52-53 -0.4390475515211776 2316 54-55 -0.5309508526531985 2316 56-57 -0.6663269853374558 2316 58-59 -0.5648505565682598 2316 60-61 -0.592407539599705 2316 62-63 -0.7500642125000283 2316 64-65 -0.6568485376716993 2316 66-67 -0.6793115820227698 2316 68-69 -0.6998781553116302 2316 70-71 -1.0385198779425018 2316 72-73 -0.9236173756520429 2316 74-75 -0.8570370984132332 2316 76-77 -0.9062758294444322 2316 78-79 -1.1074710545527466 2316 80-81 -1.3832401263816507 2316 82-83 -1.4335239092982768 2316 84-85 -1.5242417311329817 2316 86-87 -1.5832497626328 2316 88-89 -1.7866256817396753 2316 90-91 -1.9811004898685418 2316 92-93 -2.4622866007384054 2316 94-95 -2.2543736353957513 2316 96-97 -2.4658489193510853 2316 98-99 -2.993904693269922 2316 100-101 -3.601016880001776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 7.0 5 33.0 6 64.0 7 165.0 8 456.0 9 858.0 10 1383.0 11 2246.0 12 3408.0 13 4991.0 14 7211.0 15 9678.0 16 12771.0 17 17551.0 18 18014.0 19 14165.0 20 13093.0 21 13236.0 22 13912.0 23 15218.0 24 17209.0 25 19649.0 26 23228.0 27 28309.0 28 34000.0 29 40960.0 30 49403.0 31 60795.0 32 76302.0 33 99160.0 34 136825.0 35 201356.0 36 302196.0 37 391887.0 38 225254.0 39 13626.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.634908997500844 18.345579381686047 14.724851896844212 23.2946597239689 2 16.283436112125045 19.361768858507805 39.217753667013845 25.13704136235331 3 20.234914073383486 22.7561702808597 29.04475376944126 27.96416187631555 4 13.53927743855124 16.231570722936052 35.42028116855664 34.808870669956065 5 14.39089470017007 36.46154982323911 33.67024755087428 15.477307925716543 6 33.921518889795074 35.050422020862364 16.25866678654071 14.769392302801842 7 29.830356999513015 31.919695177750544 19.350379689933266 18.899568132803175 8 27.33087877214858 34.353135719751904 18.643230602098864 19.67275490600065 9 26.233336722625666 16.66226058663299 19.568935530308302 37.53546716043304 10-11 25.783140589629838 25.927845533893816 26.08978235391704 22.19923152255931 12-13 26.649390194955664 23.043406131764982 26.837067798333536 23.470135874945814 14-15 23.175909623628005 24.625474277946946 25.140049126900454 27.058566971524595 16-17 22.83962047061216 27.755227091505542 26.155177857574802 23.249974580307498 18-19 22.8627657695745 26.02652745594366 28.585246945623265 22.525459828858576 20-21 23.137164660740755 25.20490947913712 29.083767251943936 22.574158608178184 22-23 23.005838502004142 25.138229612068734 28.999320357695208 22.856611528231912 24-25 22.883937633338856 25.332622995518822 28.9309692391795 22.85247013196282 26-27 22.332822513607734 25.72494739698722 28.856104599036847 23.086125490368204 28-29 22.5719152552441 26.15781781557864 28.60801777565619 22.66224915352107 30-31 22.60928269048663 25.61710366720833 28.599317627875205 23.174296014429835 32-33 22.26749464629131 25.6719529885107 28.63885619147747 23.421696173720523 34-35 23.146465116826757 25.77087889560793 28.43139458829902 22.651261399266293 36-37 23.161308709072735 25.329645848971023 28.350198623749485 23.158846818206754 38-39 22.61506275590726 25.752630630963363 28.301673696045537 23.33063291708384 40-41 23.1731003157877 25.54082557647514 28.373574251593325 22.912499856143835 42-43 22.818964017609826 25.636857529004025 28.21439563233464 23.329782821051502 44-45 22.837969613348378 25.916346794030567 28.308166332343564 22.93751726027749 46-47 23.30175517274781 26.097283996337346 27.616048669826625 22.98491216108822 48-49 23.014028225559375 26.16391031803858 27.578104869060173 23.24395658734187 50-51 23.15862423272665 26.476241952660505 27.489765229071565 22.875368585541278 52-53 23.52702247100817 26.118065106521893 27.412141515441796 22.94277090702814 54-55 23.053627523907892 26.36410097237013 27.604153845330536 22.978117658391444 56-57 23.06007609853208 26.440868443726153 27.448799387786778 23.050256069954994 58-59 23.039526283962047 26.001509127007488 27.443046510010007 23.51591807902046 60-61 22.746343577915372 26.347912641882022 27.67597116604143 23.229772614161178 62-63 22.82185344343182 26.363806639088534 27.773101148970103 23.04123876850955 64-65 22.56738894270134 26.43977138331291 27.608113965846638 23.38472570813912 66-67 22.528092217663012 26.46141743450055 27.519737132566902 23.490753215269535 68-69 23.039573504354006 26.02824324208616 27.719407435631783 23.21277581792806 70-71 22.78270711697875 26.27732616943964 27.269898267714847 23.670068445866757 72-73 22.825412200382097 26.34432712736069 27.29419414223255 23.536066530024673 74-75 22.77173651284631 26.68966033939303 27.131481352648734 23.407121795111927 76-77 22.860143527611136 26.314305132637276 27.34334780026008 23.4822035394915 78-79 22.951012238913002 26.460749319019815 27.348485253902595 23.239753188164592 80-81 23.01034798591156 26.560526205238133 27.123747976118263 23.305377832732045 82-83 22.86794615674012 26.620791187455485 27.212728826629863 23.298533829174538 84-85 22.824146062974954 26.683877997566547 27.158554301722234 23.333421637736272 86-87 23.10959851997018 26.70186571736975 26.854545970242068 23.333989792418006 88-89 22.808077051204695 26.9044525995684 26.672036026441866 23.61543432278504 90-91 22.836859695645543 26.853567883031985 26.7414485602434 23.568123861079073 92-93 22.89417176067333 26.41220046371181 26.97927746047546 23.714350315139402 94-95 22.673887652836104 26.281776330488555 27.142622058168364 23.901713958506978 96-97 22.98574939942407 25.972868791618215 27.102604431177397 23.938777377780323 98-99 22.658761036183805 26.268231310064188 26.752303564223123 24.32070408952888 100-101 23.036291549364744 25.811373423230695 26.923022377206596 24.229312650197972 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 588.0 1 418.5 2 376.0 3 508.0 4 393.5 5 345.0 6 738.0 7 1708.0 8 2737.0 9 3125.0 10 2555.0 11 1814.0 12 1693.5 13 1814.0 14 1960.0 15 2078.0 16 2221.0 17 2421.5 18 2566.5 19 2793.5 20 3148.0 21 3636.0 22 4381.5 23 5354.5 24 6507.0 25 7978.5 26 9926.5 27 12158.5 28 14346.0 29 16440.0 30 18706.0 31 21799.0 32 26928.5 33 29238.5 34 28609.0 35 30076.0 36 32989.0 37 36233.5 38 39102.0 39 41951.5 40 45444.5 41 49752.0 42 52010.5 43 54173.5 44 57443.0 45 61395.0 46 64180.5 47 66801.5 48 69204.0 49 68897.5 50 69201.0 51 68922.0 52 68962.5 53 71152.5 54 73440.0 55 70837.0 56 66297.0 57 62322.0 58 58566.0 59 52562.5 60 42790.0 61 35444.0 62 29674.0 63 22163.5 64 15862.0 65 12288.0 66 9365.5 67 7143.0 68 5484.0 69 4157.5 70 3108.0 71 2149.0 72 1468.5 73 1147.0 74 885.0 75 643.5 76 447.5 77 287.0 78 197.0 79 115.5 80 64.0 81 44.5 82 24.5 83 14.0 84 10.5 85 6.0 86 3.0 87 2.5 88 2.5 89 1.5 90 2.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011559270695643333 3 0.006261271626806805 4 0.0 5 2.140605684378395E-4 6 1.0703028421891974E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018997875448858255 26-27 0.020924420564798808 28-29 3.746059947662191E-4 30-31 8.02727131641898E-5 32-33 0.003505241808169622 34-35 0.01648266376971364 36-37 0.007893483461145332 38-39 0.02137929927272922 40-41 0.023787480667654914 42-43 0.018997875448858254 44-45 0.009525695295483857 46-47 0.002087090542268935 48-49 2.4081813949256942E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.01363565820949E-4 68-69 3.210908526567592E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.943332816020293E-4 82-83 0.0011238179842986574 84-85 0.005003665787234498 86-87 0.0010167877000797376 88-89 1.3378785527364968E-4 90-91 0.004548787079304089 92-93 6.689392763682484E-4 94-95 1.3378785527364968E-4 96-97 3.746059947662191E-4 98-99 2.140605684378395E-4 100-101 0.002916575244965563 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1868630.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.57161554290502 #Duplication Level Percentage of deduplicated Percentage of total 1 78.97704146896648 45.46835868167405 2 12.387577132639253 14.263456563767777 3 3.6784851853573124 6.353290046149886 4 1.5723555554754132 3.6209219814632543 5 0.8624227169758971 2.4825534548601964 6 0.5378526151192626 1.857902638583535 7 0.3617948225206071 1.4580378699298961 8 0.25106452349683894 1.1563352178578126 9 0.186183832384165 0.9647013616493236 >10 1.0696057394635041 11.247769416291135 >50 0.07204449453657052 2.828483937953316 >100 0.037893647028733886 4.053489664362813 >500 0.002978762510675638 1.202267294714523 >1k 0.0025133308683825692 2.264286149103295 >5k 1.8617265691722737E-4 0.7781457216392473 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7791 0.41693647217480184 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6729 0.3601033912545555 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4145 0.22182026404371116 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2613 0.13983506633201864 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2268 0.12137234230425499 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2145 0.11478997982479143 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2067 0.11061579874025355 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2061 0.1102947078875968 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1906 0.10199986086063051 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 5.351514210945987E-5 3 0.0 0.0 0.0 0.0 5.351514210945987E-5 4 0.0 0.0 0.0 0.0 5.351514210945987E-5 5 0.0 0.0 0.0 0.0 1.605454263283796E-4 6 0.0 0.0 0.0 0.0 1.605454263283796E-4 7 0.0 0.0 0.0 0.0 1.605454263283796E-4 8 0.0 0.0 0.0 0.0 1.605454263283796E-4 9 0.0 0.0 0.0 0.0 1.605454263283796E-4 10-11 0.0 0.0 0.0 0.0 1.605454263283796E-4 12-13 0.0 0.0 0.0 0.0 5.083938500398688E-4 14-15 5.351514210945987E-5 0.0 0.0 0.0 6.421817053135184E-4 16-17 5.351514210945987E-5 0.0 0.0 5.351514210945987E-5 8.027271316418982E-4 18-19 5.351514210945987E-5 0.0 0.0 5.351514210945987E-5 8.56242273751358E-4 20-21 5.351514210945987E-5 0.0 0.0 1.0703028421891974E-4 9.097574158608179E-4 22-23 5.351514210945987E-5 0.0 0.0 1.8730299738310956E-4 9.097574158608179E-4 24-25 5.351514210945987E-5 0.0 0.0 2.4081813949256942E-4 9.097574158608179E-4 26-27 5.351514210945987E-5 0.0 0.0 6.956968474229783E-4 9.632725579702777E-4 28-29 5.351514210945987E-5 0.0 0.0 0.0028898176739108328 9.632725579702777E-4 30-31 5.351514210945987E-5 0.0 0.0 0.009712998292866967 9.632725579702777E-4 32-33 5.351514210945987E-5 0.0 0.0 0.021967965835933276 0.0010167877000797376 34-35 5.351514210945987E-5 0.0 0.0 0.04214317441119965 0.0010167877000797376 36-37 5.351514210945987E-5 0.0 0.0 0.07754344091660736 0.0010167877000797376 38-39 5.351514210945987E-5 0.0 0.0 0.13137967387872398 0.0011238179842986574 40-41 5.351514210945987E-5 0.0 0.0 0.18660730053568658 0.0011238179842986574 42-43 1.0703028421891974E-4 0.0 0.0 0.24103220006100726 0.0012040906974628471 44-45 1.0703028421891974E-4 0.0 0.0 0.3111905513665092 0.001230848268517577 46-47 1.0703028421891974E-4 0.0 0.0 0.3929349309387091 0.0012576058395723068 48-49 1.0703028421891974E-4 0.0 0.0 0.478746461311228 0.0013646361237912266 50-51 1.0703028421891974E-4 0.0 0.0 0.5719698388659071 0.0014984239790648765 52-53 1.0703028421891974E-4 0.0 0.0 0.6586376115121774 0.0015251815501196064 54-55 1.0703028421891974E-4 0.0 0.0 0.7518342314958018 0.001605454263283796 56-57 1.0703028421891974E-4 0.0 0.0 0.8634668179361351 0.001658969405393256 58-59 1.0703028421891974E-4 0.0 0.0 0.9744572226711548 0.001712484547502716 60-61 1.0703028421891974E-4 0.0 0.0 1.1009402610468633 0.0017659996896121758 62-63 1.0703028421891974E-4 0.0 0.0 1.222928027485377 0.0019265451159405553 64-65 1.0703028421891974E-4 0.0 0.0 1.3484477933031151 0.0019800602580500155 66-67 1.0703028421891974E-4 0.0 0.0 1.4956679492462392 0.002006817829104745 68-69 1.0703028421891974E-4 0.0 0.0 1.6589158902511465 0.002087090542268935 70-71 1.0703028421891974E-4 0.0 0.0 1.8267661334774674 0.002113848113323665 72-73 1.0703028421891974E-4 0.0 0.0 2.0029647388728637 0.002274393539652045 74-75 1.0703028421891974E-4 0.0 0.0 2.1693968308332843 0.0024349389659804243 76-77 1.0703028421891974E-4 0.0 0.0 2.3532481015503337 0.002515211679144614 78-79 1.0703028421891974E-4 0.0 0.0 2.560084125803396 0.0025419692501993437 80-81 1.0703028421891974E-4 0.0 0.0 2.7818776322760526 0.002595484392308804 82-83 2.140605684378395E-4 0.0 0.0 3.0055441687225404 0.002836302531801373 84-85 2.4081813949256942E-4 0.0 0.0 3.2299866747296146 0.002863060102856103 86-87 2.6757571054729937E-4 0.0 0.0 3.465881421148114 0.0030503631002392127 88-89 2.6757571054729937E-4 0.0 0.0 3.7183123464784362 0.003210908526567592 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3760 0.0 50.40854 1 GTATCAA 6300 0.0 39.962658 1 TATCAAC 8185 0.0 30.294994 2 ATCAACG 8260 0.0 29.9033 3 TCAACGC 8300 0.0 29.756798 4 CAACGCA 8430 0.0 29.072546 5 AACGCAG 8730 0.0 28.07349 6 GTGGTAT 1875 0.0 27.361603 1 TGGTATC 1810 0.0 26.507076 2 ACGCAGA 10005 0.0 24.258545 7 CGCAGAG 10130 0.0 23.818544 8 TAAGGTG 1155 0.0 23.028572 5 TAGGACG 145 2.3367127E-5 22.92931 4 GTAAGGT 1105 0.0 22.781092 4 TACATGG 6200 0.0 21.835957 2 GTACATG 6310 0.0 21.681145 1 GCAGAGT 11160 0.0 21.49256 9 GAGTACT 7165 0.0 20.814903 12-13 ACATGGG 6280 0.0 20.346441 3 GTACTTT 7370 0.0 20.26815 14-15 >>END_MODULE