FastQCFastQC Report
Thu 26 May 2016
SRR938257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887087
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81460.4316706119007762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67310.3566873175428584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40740.21588829767784953No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24760.13120751719449078No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23530.12468953471673537No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22040.1167937673249829No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA20810.11027578484722751No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC19940.10566550455808343No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA19190.10169112499847648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40150.052.797551
GTATCAA68250.040.92321
TATCAAC87000.032.0594982
ATCAACG86750.032.0423433
TCAACGC87650.031.713334
CAACGCA89050.031.214755
AACGCAG92700.030.0882026
GTGGTAT17600.027.6696621
TAAGGTG11750.027.0941055
ACGCAGA103200.026.8109157
TGGTATC17900.026.2796782
CGCAGAG105100.026.2343648
GTAAGGT12200.024.536844
GCAGAGT116700.023.381019
GTGTAAG7700.022.9417931
GAGTACT73150.022.646712-13
TACGACG1903.2787466E-722.5074755
GTACTTT74850.022.14852514-15
AGGTAAG11600.022.1194152
CAGAGTA108800.021.9335310-11
Produced by FastQC (version 0.11.5)