##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938257_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1887087 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.800956182730314 33.0 31.0 34.0 30.0 34.0 2 31.987867543997705 34.0 31.0 34.0 30.0 34.0 3 32.058506576538335 34.0 31.0 34.0 30.0 34.0 4 35.58651296946034 37.0 35.0 37.0 33.0 37.0 5 35.60398063258345 37.0 35.0 37.0 33.0 37.0 6 35.627105692530336 37.0 35.0 37.0 33.0 37.0 7 35.56148656633213 37.0 35.0 37.0 33.0 37.0 8 35.53868210633638 37.0 35.0 37.0 33.0 37.0 9 37.18891656823453 39.0 37.0 39.0 34.0 39.0 10-11 37.104039718359566 39.0 37.0 39.0 33.0 39.0 12-13 36.999993111075426 39.0 37.0 39.0 33.0 39.0 14-15 38.23175958501119 40.0 38.0 41.0 33.0 41.0 16-17 38.14681861514599 40.0 38.0 41.0 33.0 41.0 18-19 38.07435799197387 40.0 38.0 41.0 33.0 41.0 20-21 37.95767656711111 40.0 38.0 41.0 33.0 41.0 22-23 37.80504820392488 40.0 38.0 41.0 32.5 41.0 24-25 37.745114560166016 40.0 38.0 41.0 32.0 41.0 26-27 37.7455896310027 40.0 38.0 41.0 32.0 41.0 28-29 37.64485394685036 40.0 38.0 41.0 32.0 41.0 30-31 37.45587829283971 40.0 37.0 41.0 32.0 41.0 32-33 37.21938919615259 40.0 37.0 41.0 31.0 41.0 34-35 37.008849353527424 40.0 36.0 41.0 31.0 41.0 36-37 36.77877411057359 40.0 36.0 41.0 30.0 41.0 38-39 36.52264018564062 39.0 35.5 41.0 30.0 41.0 40-41 36.272844336270666 39.0 35.0 41.0 30.0 41.0 42-43 35.96126490193616 39.0 35.0 40.5 28.5 41.0 44-45 35.72332542166842 39.0 35.0 40.0 28.0 41.0 46-47 35.58012985092897 39.0 35.0 41.0 27.0 41.0 48-49 35.31120186827634 39.0 35.0 41.0 26.0 41.0 50-51 34.64786122738379 38.5 34.0 39.5 24.5 40.5 52-53 34.712081901894294 38.0 34.0 40.0 25.0 41.0 54-55 34.83037798469281 38.5 34.5 40.0 25.0 41.0 56-57 34.595847197294034 38.0 34.0 40.0 24.0 41.0 58-59 34.30378143667992 38.0 34.0 40.0 23.0 41.0 60-61 33.90387804059908 37.5 34.0 40.0 22.5 41.0 62-63 33.46069285623821 37.0 33.0 40.0 21.5 41.0 64-65 33.008862601459285 36.0 33.0 39.0 19.5 41.0 66-67 32.55558249301701 35.5 32.0 39.0 18.0 41.0 68-69 32.05344348193803 35.0 31.5 38.5 14.0 40.0 70-71 31.554319965110246 35.0 31.0 38.0 9.5 40.0 72-73 31.06251805030717 35.0 31.0 37.0 7.0 39.5 74-75 30.534138595623837 35.0 30.0 36.5 4.0 39.0 76-77 29.994491244971748 34.5 29.5 36.0 2.0 39.0 78-79 29.435709641367886 34.0 29.0 35.5 2.0 37.5 80-81 28.910429672823774 34.0 29.0 35.0 2.0 37.0 82-83 28.371931977698964 34.0 27.5 35.0 2.0 36.5 84-85 27.887107748609363 34.0 26.5 35.0 2.0 36.0 86-87 27.393666269758626 33.0 26.0 35.0 2.0 36.0 88-89 26.824862340739987 33.0 25.0 35.0 2.0 35.0 90-91 26.200524936052233 32.5 24.0 35.0 2.0 35.0 92-93 25.572680008923804 32.0 21.5 34.5 2.0 35.0 94-95 24.9462875850451 32.0 18.5 34.0 2.0 35.0 96-97 24.079659019430476 31.0 6.0 34.0 2.0 35.0 98-99 22.979453782470017 31.0 2.0 34.0 2.0 35.0 100-101 20.146023209316795 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3964803531062451 1101 2 0.32887943411586207 1101 3 0.27913114193446376 1101 4 0.2097265806758628 1101 5 0.18427318122671466 1101 6 0.2161936072638042 1101 7 0.2795300749174814 1101 8 0.2994290772109167 1101 9 0.21517843787074042 1101 10-11 0.27533154851870023 1101 12-13 0.13947281375968146 1101 14-15 0.20731970896242302 1101 16-17 0.35947182216023066 1101 18-19 0.4390669845595241 1101 20-21 0.3849369268829932 1101 22-23 0.5683498182677837 1101 24-25 0.4441349787225448 1101 26-27 0.42458410104725886 1101 28-29 0.36363302618668314 1101 30-31 0.4869478193201644 1101 32-33 0.4412216598565166 1101 34-35 0.5289316757935723 1101 36-37 0.5590352907337959 1101 38-39 0.49345805813302945 1101 40-41 0.6218537124241124 1101 42-43 0.5459178090466281 1101 44-45 0.7008437496232318 1101 46-47 0.6136768123045826 1101 48-49 0.7219258091893508 1101 50-51 0.8372597878923145 1101 52-53 0.602375464176923 1101 54-55 0.7074801828996087 1101 56-57 0.7056389450210361 1101 58-59 0.8271236535109381 1101 60-61 0.8258342674164041 1101 62-63 0.8550803697183369 1101 64-65 0.9077768077632911 1101 66-67 0.6410501150019101 1101 68-69 0.7059900070472835 1101 70-71 0.6843617749543718 1101 72-73 0.8836181796129026 1101 74-75 0.988582166889362 1101 76-77 0.9977795760506893 1101 78-79 0.9793374763300591 1101 80-81 1.1179767622368715 1101 82-83 0.9043485652975782 1101 84-85 0.9381466304679513 1101 86-87 1.0114511680699536 1101 88-89 0.9060222212351263 1101 90-91 0.9135431012234889 1101 92-93 0.882156741335919 1101 94-95 1.0183360443200797 1101 96-97 1.0552179801754171 1101 98-99 0.9576851985430039 1101 100-101 1.055137522928682 1102 1 0.2128784820043741 1102 2 0.1757922341536613 1102 3 0.13122668153000205 1102 4 0.04409596973275143 1102 5 0.04651271534125101 1102 6 0.15946254272024163 1102 7 0.061140356527765505 1102 8 0.2175506515824921 1102 9 0.12892642974372848 1102 10-11 0.20328703897418876 1102 12-13 0.18870440986627557 1102 14-15 0.2876994221546383 1102 16-17 0.14202267502358268 1102 18-19 0.15898494666499374 1102 20-21 0.2572606546754699 1102 22-23 0.23772573373744876 1102 24-25 0.08374410270666743 1102 26-27 0.1938303741997771 1102 28-29 0.26893433383174425 1102 30-31 0.26616754385239005 1102 32-33 0.24386928672289798 1102 34-35 0.07160363643427559 1102 36-37 0.15943868801219452 1102 38-39 0.11366586974708781 1102 40-41 0.1814507876461846 1102 42-43 0.22272600929233022 1102 44-45 0.18993194183642004 1102 46-47 0.1161286467876721 1102 48-49 0.12658259665864335 1102 50-51 0.15329689689816917 1102 52-53 0.10598935450956048 1102 54-55 0.10509302191869807 1102 56-57 0.20834327194411628 1102 58-59 0.0940391704264556 1102 60-61 0.2862498020506976 1102 62-63 0.4044736495803605 1102 64-65 0.2540487790352657 1102 66-67 0.229253402423943 1102 68-69 0.16068754768232907 1102 70-71 0.3121997309173281 1102 72-73 0.2805547523307226 1102 74-75 0.16170264860359396 1102 76-77 0.08726378272239543 1102 78-79 0.042531461399043735 1102 80-81 0.037619197504305646 1102 82-83 0.19346938957464843 1102 84-85 0.18099415456547874 1102 86-87 0.142342658336446 1102 88-89 0.11972237243527672 1102 90-91 0.2026041246595156 1102 92-93 0.36480416148333816 1102 94-95 0.2718576494041862 1102 96-97 0.37171873026991875 1102 98-99 0.5551494952260505 1102 100-101 0.6342739692526322 1103 1 0.38755611329029094 1103 2 0.3114926205507018 1103 3 0.31325921905617093 1103 4 0.11509677426261788 1103 5 0.17536067868363858 1103 6 0.1714041739393437 1103 7 0.1600446369607056 1103 8 0.17324165643751144 1103 9 0.15050606428116708 1103 10-11 0.22239253538860027 1103 12-13 0.08267419406296739 1103 14-15 0.2933460943518682 1103 16-17 0.201656363389354 1103 18-19 0.2774251748285437 1103 20-21 0.30857814192548716 1103 22-23 0.26482589770564857 1103 24-25 0.4609857901593486 1103 26-27 0.1642748630310109 1103 28-29 0.30820962362952997 1103 30-31 0.23960239920404547 1103 32-33 0.3678519670600764 1103 34-35 0.3658696560436425 1103 36-37 0.28620433876272244 1103 38-39 0.3439623696246059 1103 40-41 0.3781311972047803 1103 42-43 0.4205989464666189 1103 44-45 0.41100157681927385 1103 46-47 0.41541079875194953 1103 48-49 0.48008125763143283 1103 50-51 0.4111224461498679 1103 52-53 0.6171624717983235 1103 54-55 0.5790646364904362 1103 56-57 0.5057981806898226 1103 58-59 0.5921962681058446 1103 60-61 0.45155024106231423 1103 62-63 0.4474507511014565 1103 64-65 0.32753102926910316 1103 66-67 0.4420016165903533 1103 68-69 0.5334051465643341 1103 70-71 0.6574286461104144 1103 72-73 0.6902955141564782 1103 74-75 0.5615840918021142 1103 76-77 0.5862883946550497 1103 78-79 0.7439151692517036 1103 80-81 0.49277918480999006 1103 82-83 0.5444857348933461 1103 84-85 0.5882017956505052 1103 86-87 0.6096471492277189 1103 88-89 0.49259830223795475 1103 90-91 0.5838489745548401 1103 92-93 0.7302138314312252 1103 94-95 0.8016972862800493 1103 96-97 0.5163108767778652 1103 98-99 0.6416728196325359 1103 100-101 0.6414886395345789 1104 1 0.2817923868246588 1104 2 0.20671592948223605 1104 3 0.18295110252733338 1104 4 0.06396669288410095 1104 5 0.10131753452604642 1104 6 0.1817494254092651 1104 7 0.11816500518587958 1104 8 0.19700235028219737 1104 9 0.047491865801696065 1104 10-11 0.18948784016502884 1104 12-13 0.10955055823981041 1104 14-15 0.13400957987540352 1104 16-17 0.2592573763282573 1104 18-19 0.2572973974114561 1104 20-21 0.23838064586455943 1104 22-23 0.24802091975163165 1104 24-25 0.3811636279025876 1104 26-27 0.2915263006587807 1104 28-29 0.3243909072633002 1104 30-31 0.3298253439705974 1104 32-33 0.34743219853717733 1104 34-35 0.378379794165987 1104 36-37 0.49887421513447805 1104 38-39 0.41027219855274666 1104 40-41 0.5319177385239939 1104 42-43 0.40737580297474096 1104 44-45 0.3435461936204973 1104 46-47 0.30960422934754206 1104 48-49 0.3427047153268248 1104 50-51 0.46430338605144783 1104 52-53 0.46656542249038324 1104 54-55 0.47265727725996953 1104 56-57 0.4424773058633775 1104 58-59 0.34200883024870876 1104 60-61 0.27663356233920666 1104 62-63 0.3101262794455195 1104 64-65 0.327524108486692 1104 66-67 0.5039614042080203 1104 68-69 0.4852310631807768 1104 70-71 0.52533414381519 1104 72-73 0.39626310709846635 1104 74-75 0.22120485269929802 1104 76-77 0.30437576063492244 1104 78-79 0.35728549471632576 1104 80-81 0.42258004094373547 1104 82-83 0.5006857507781852 1104 84-85 0.5880103206702891 1104 86-87 0.4314875896004011 1104 88-89 0.6164743754123805 1104 90-91 0.6082117764698545 1104 92-93 0.6836359770781009 1104 94-95 0.7339013017180065 1104 96-97 0.7108934530819653 1104 98-99 0.9986055815948625 1104 100-101 1.1811274208309328 1105 1 0.42355135035078106 1105 2 0.297196893756535 1105 3 0.2619521111979495 1105 4 0.2890888259817075 1105 5 0.26919699283165244 1105 6 0.17909873285874056 1105 7 0.2852768491024378 1105 8 0.3468942607932348 1105 9 0.2720439209835135 1105 10-11 0.3256068287289082 1105 12-13 0.26991113020667257 1105 14-15 0.38492874177752867 1105 16-17 0.4622308866871876 1105 18-19 0.4180045841587301 1105 20-21 0.325707045256145 1105 22-23 0.377215946277083 1105 24-25 0.5110316673654367 1105 26-27 0.42575264288825565 1105 28-29 0.48512340337121174 1105 30-31 0.4607708831649191 1105 32-33 0.6104916743000715 1105 34-35 0.593756434490345 1105 36-37 0.6210892595122814 1105 38-39 0.5035745392364959 1105 40-41 0.675550675014783 1105 42-43 0.4842329236090279 1105 44-45 0.649627107029147 1105 46-47 0.8148585452108321 1105 48-49 0.6661196085892342 1105 50-51 0.6009878227700156 1105 52-53 0.49585761226861536 1105 54-55 0.6520390683478681 1105 56-57 0.8094892704006469 1105 58-59 0.6201738275264077 1105 60-61 0.6356437564558277 1105 62-63 0.5804830303162234 1105 64-65 0.486236331179434 1105 66-67 0.570888304601489 1105 68-69 0.6421072769498011 1105 70-71 0.7601937447668128 1105 72-73 0.8139407078204641 1105 74-75 0.7805750570384333 1105 76-77 0.749357840372987 1105 78-79 0.7778346275929344 1105 80-81 0.883817366201118 1105 82-83 0.9708248720124928 1105 84-85 1.0200824659287342 1105 86-87 0.9473625774086969 1105 88-89 0.7791688099457019 1105 90-91 0.7535567986124576 1105 92-93 1.010275961917177 1105 94-95 1.2407653855129794 1105 96-97 1.0904367919668978 1105 98-99 0.9948780066143392 1105 100-101 0.8119695620275351 1106 1 -0.015285835031811246 1106 2 0.025150995065096993 1106 3 -0.04622762362142652 1106 4 0.04680814085278939 1106 5 0.07122161559124152 1106 6 0.15695720226288756 1106 7 0.16648439980468055 1106 8 0.1011786278949387 1106 9 0.10896118754054385 1106 10-11 -0.5565460621282199 1106 12-13 -0.298727585748658 1106 14-15 -0.06508293051533798 1106 16-17 -0.09854347749065795 1106 18-19 0.08332686656581245 1106 20-21 -0.009847538892415741 1106 22-23 -0.24643235020378285 1106 24-25 -0.031948517472834226 1106 26-27 0.07685018511941166 1106 28-29 -0.21967925398227806 1106 30-31 -0.11409575222154444 1106 32-33 -0.12782606515816752 1106 34-35 -0.31452375716639835 1106 36-37 -0.3385666447610518 1106 38-39 -0.06864719503509065 1106 40-41 0.02598136528644801 1106 42-43 -0.23798673045194363 1106 44-45 -0.14126029926025296 1106 46-47 -0.016667364390151818 1106 48-49 -0.10801300137012504 1106 50-51 -0.04641907913764953 1106 52-53 -0.0951417786919464 1106 54-55 -0.4466618690181505 1106 56-57 -0.3790041023950579 1106 58-59 -0.1653405681088529 1106 60-61 -0.4790001172661391 1106 62-63 -0.2685297941821787 1106 64-65 -0.3008977395500452 1106 66-67 -0.43795750209841344 1106 68-69 -0.5865785095489358 1106 70-71 -0.3904936781642654 1106 72-73 -0.30095365792909945 1106 74-75 -0.42069167565565735 1106 76-77 -0.28323877096392636 1106 78-79 -0.06218056372287961 1106 80-81 -0.15330471442553417 1106 82-83 -0.020928681969550667 1106 84-85 -0.03256650893192514 1106 86-87 -0.0158892020381316 1106 88-89 -0.2637941842632223 1106 90-91 -0.4092105806804298 1106 92-93 -0.31517053217675794 1106 94-95 -0.42983586469359736 1106 96-97 -0.012241103599599512 1106 98-99 -0.06549057203553588 1106 100-101 -0.13276458705653482 1107 1 0.27269878465255104 1107 2 0.10724321145504945 1107 3 0.061909216208441364 1107 4 0.11214254917789646 1107 5 0.17551255771199692 1107 6 0.1139372432246546 1107 7 0.07686387481275858 1107 8 0.15382902484856942 1107 9 0.1144048345356623 1107 10-11 0.25948186873459633 1107 12-13 -0.0150905144716873 1107 14-15 0.01867554132235938 1107 16-17 0.018273857243009672 1107 18-19 0.11477979218832246 1107 20-21 0.03730380568429581 1107 22-23 0.20721954678915466 1107 24-25 0.2421812086335322 1107 26-27 0.24668050818465304 1107 28-29 0.2869385504143409 1107 30-31 0.2784921813051042 1107 32-33 0.40456738729036346 1107 34-35 0.5056505705452494 1107 36-37 0.3718638141156063 1107 38-39 0.39086184231959464 1107 40-41 0.46126242068528 1107 42-43 0.4505692469972473 1107 44-45 0.4245039784064062 1107 46-47 0.38120687030595235 1107 48-49 0.526556981904541 1107 50-51 0.47889490288807934 1107 52-53 0.43671031131913907 1107 54-55 0.5664196691444232 1107 56-57 0.633661486004641 1107 58-59 0.503264422633265 1107 60-61 0.4447805003482941 1107 62-63 0.394380451282764 1107 64-65 0.44300684965316606 1107 66-67 0.46119992669884624 1107 68-69 0.3704625319194008 1107 70-71 0.687692322342297 1107 72-73 0.7260987703004602 1107 74-75 0.593680698729699 1107 76-77 0.6146420141221434 1107 78-79 0.46084009948227234 1107 80-81 0.5713912656001412 1107 82-83 0.4877878400790614 1107 84-85 0.5387534546915766 1107 86-87 0.7912826015899128 1107 88-89 0.9559061681477594 1107 90-91 0.8407858711916703 1107 92-93 0.8399578500079308 1107 94-95 0.945200571850588 1107 96-97 0.8441089809004332 1107 98-99 0.8686100485047383 1107 100-101 0.8648954225943726 1108 1 0.11828481270318036 1108 2 0.13631217898502257 1108 3 0.09545021232271012 1108 4 0.053063929862652515 1108 5 0.0395787318527141 1108 6 0.14660269942264392 1108 7 0.08074282933471721 1108 8 0.09934646893908905 1108 9 0.16545975590383932 1108 10-11 0.09846173965499361 1108 12-13 0.04600080434158116 1108 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-1.011634377378428 2316 64-65 -0.9207003848443307 2316 66-67 -1.0008959678475122 2316 68-69 -1.0681296362154526 2316 70-71 -1.232114142998963 2316 72-73 -1.1848668745728688 2316 74-75 -1.2516245677242956 2316 76-77 -1.176336618696574 2316 78-79 -1.1448980163098241 2316 80-81 -1.0090670665798598 2316 82-83 -1.0720489391180799 2316 84-85 -1.1840647391579395 2316 86-87 -1.004309714459609 2316 88-89 -0.900894489596741 2316 90-91 -1.1079887293522432 2316 92-93 -1.190097695840425 2316 94-95 -1.2417452887878468 2316 96-97 -1.3298833180221266 2316 98-99 -1.4171703786658902 2316 100-101 -1.3477852106187846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5630.0 3 1695.0 4 1802.0 5 1950.0 6 2384.0 7 2719.0 8 3433.0 9 4353.0 10 5208.0 11 6433.0 12 7826.0 13 9202.0 14 10771.0 15 12887.0 16 15682.0 17 20118.0 18 18415.0 19 15367.0 20 14990.0 21 15937.0 22 16805.0 23 18291.0 24 20291.0 25 22638.0 26 25420.0 27 29594.0 28 34240.0 29 40444.0 30 48604.0 31 58796.0 32 73277.0 33 94994.0 34 129552.0 35 190066.0 36 280134.0 37 359708.0 38 241331.0 39 26100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44942676136146 17.850972865426318 14.316374047265027 23.383226325947195 2 16.058111657429215 19.04300903103763 39.757984733018695 25.140894578514466 3 20.01087997353176 22.7334183795202 28.84629863512535 28.409403011822693 4 13.222791663242067 15.841324803207016 35.678852940162734 35.25703059338818 5 14.420585380858183 36.45509941080075 33.56738668123313 15.556928527107939 6 33.8338745091091 34.787249223247876 16.492448913661022 14.886427353982004 7 29.840150855508973 31.923269865275767 19.43319945401845 18.803379825196814 8 27.259289421056092 34.1559727780358 18.82776530069073 19.756972500217376 9 26.4064453396305 16.759378157907854 19.598459921808477 37.23571658065317 10-11 25.788256219265605 25.86151763285293 26.157188288992018 22.19303785888945 12-13 26.754483800918948 23.058058454987464 26.77970804069723 23.40774970339636 14-15 23.22967699518568 24.655495111450445 25.096259888443512 27.01856800492036 16-17 22.908608782725647 27.764058000062036 26.06786928803235 23.259463929179965 18-19 22.966221513129657 26.08876506280427 28.43144818458959 22.513565239476485 20-21 23.25822869726269 25.223517495469196 29.02210184268197 22.49615196458614 22-23 23.017157613284922 25.098553871565134 28.947808669347086 22.93647984580286 24-25 22.97142769250679 25.32089547884909 28.852164522943607 22.85551230570051 26-27 22.3815722841894 25.739810324759855 28.7502750741706 23.12834231688014 28-29 22.606031184371385 26.16358100876078 28.551471658513393 22.678916148354443 30-31 22.662440654561692 25.690098691205392 28.50100303269574 23.146457621537177 32-33 22.317252562876106 25.672297605436924 28.597810339744147 23.412639491942823 34-35 23.18888114359744 25.75809360124323 28.40838856604075 22.644636689118585 36-37 23.213553278752634 25.321429830819987 28.275564386319772 23.189452504107607 38-39 22.656439688324028 25.767686042332205 28.229201014887202 23.346673254456565 40-41 23.24803307712066 25.587479128656014 28.362098832159006 22.802388962064317 42-43 22.94126191691729 25.629185135469694 28.121936776390044 23.307616171222968 44-45 22.840188827202653 25.956479002871944 28.270017705531647 22.933314464393757 46-47 23.335215045214092 26.13122687614625 27.565409365094514 22.968148713545144 48-49 23.121811025041104 26.191707949525544 27.448666315413412 23.23781471001994 50-51 23.174553535342827 26.568387241254566 27.379411145019354 22.877648078383253 52-53 23.551014339654245 26.189010355283244 27.331740892383554 22.92823441267896 54-55 23.089452613378747 26.39926238419098 27.536667445838297 22.974617556591976 56-57 23.06910485612221 26.487575522025253 27.429525365091035 23.013794256761503 58-59 23.061501241197856 26.033353200626173 27.388507517548632 23.51663804062733 60-61 22.767943515792098 26.36809617581346 27.675963202574017 23.18799710582043 62-63 22.88069228753126 26.39378013977089 27.75690539798874 22.968622174709107 64-65 22.581668462587515 26.449726848654493 27.615396969178178 23.353207719579814 66-67 22.624570006208923 26.44015623273367 27.536695055995587 23.398578705061823 68-69 23.101232445221566 26.02755444982528 27.737861715755823 23.13335138919733 70-71 22.86486166970745 26.246401355457415 27.340765694543368 23.547971280291765 72-73 22.897721052886872 26.332191019232386 27.317652291391614 23.45243563648913 74-75 22.848636453751382 26.698429560270515 27.198435391242302 23.254498594735797 76-77 23.09167649004376 26.326367922928185 27.19003888361206 23.39191670341599 78-79 23.173489270487487 26.566457947820908 27.211402626152825 23.048650155538777 80-81 23.306312850183527 26.539760761530832 26.968136429368894 23.185789958916747 82-83 23.138271300665313 26.693220862269325 27.063760545818578 23.104747291246788 84-85 23.153297329842694 26.787699452209512 26.953777676722783 23.105225541225003 86-87 23.454030036472407 26.7658802293278 26.63150681591982 23.148582918279978 88-89 23.283398518522446 26.87981847990171 26.518845831174115 23.317937170401727 90-91 23.239689209633983 26.916519824430857 26.54768888833513 23.296102077600036 92-93 23.390864736266252 26.45243865720126 26.78852001221343 23.36817659431906 94-95 23.215427537084814 26.291725386276948 26.939735387962983 23.553111688675255 96-97 23.659544644381324 25.96799914327914 26.863341359789892 23.509114852549644 98-99 23.388211907365072 26.29117052645529 26.42369545770798 23.896922108471657 100-101 23.94656113147428 25.639117519278955 26.685304470392406 23.729016878854363 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 612.0 1 440.5 2 401.5 3 547.0 4 448.5 5 389.0 6 727.0 7 1642.0 8 2742.5 9 3151.0 10 2519.0 11 1824.5 12 1754.5 13 1827.0 14 1987.0 15 2144.5 16 2201.0 17 2368.5 18 2633.0 19 2942.5 20 3296.5 21 3801.5 22 4568.5 23 5523.0 24 6673.5 25 8226.0 26 10240.0 27 12541.5 28 14632.0 29 16550.5 30 18737.0 31 21891.5 32 27090.5 33 29553.0 34 29014.5 35 30468.5 36 33459.5 37 36505.0 38 39442.0 39 42234.0 40 45580.5 41 49735.0 42 52209.0 43 54477.0 44 57503.0 45 61722.5 46 64598.5 47 67192.5 48 69436.5 49 69197.5 50 69426.0 51 68805.0 52 68828.5 53 70906.0 54 73377.0 55 71175.0 56 66672.0 57 62818.0 58 59066.0 59 53156.0 60 43529.5 61 36085.0 62 30382.0 63 22991.0 64 16834.0 65 13236.0 66 10148.0 67 7769.0 68 5979.5 69 4594.0 70 3456.0 71 2422.5 72 1706.0 73 1321.0 74 961.0 75 725.0 76 546.0 77 344.0 78 237.5 79 173.5 80 103.5 81 58.5 82 36.0 83 27.5 84 20.0 85 13.5 86 13.5 87 11.5 88 8.5 89 8.5 90 6.0 91 3.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 1.0 98 1.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4946777758524117 2 0.049017347901819046 3 0.055800289016881575 4 0.06475589095786256 5 0.0782687814605262 6 0.10566550455808343 7 0.06936617124700663 8 0.05103103354535324 9 0.05028914936089327 10-11 0.07281063353199932 12-13 0.10574499214927559 14-15 0.055482338652113015 16-17 0.059536205802912114 18-19 0.05874132989099072 20-21 0.07336704667034429 22-23 0.06303365981536622 24-25 0.08796626758596715 26-27 0.06528580823247683 28-29 0.06563025446097609 30-31 0.06920719606462235 32-33 0.06968412161177519 34-35 0.07172430311904009 36-37 0.06653111382782034 38-39 0.04075063841783659 40-41 0.04390364620179144 42-43 0.04228739851421795 44-45 0.020587286118764002 46-47 0.02686680582294298 48-49 0.026972789277865835 50-51 0.024349698768525246 52-53 0.024667649133293803 54-55 0.024800128451947366 56-57 0.024826624315678083 58-59 0.021276178575762538 60-61 0.02872151628409289 62-63 0.01889155083999837 64-65 0.0214086578944161 66-67 0.015182129917698546 68-69 0.0185471046114991 70-71 0.04167799364841154 72-73 0.0377036140888046 74-75 0.032271962024008435 76-77 0.022839434535874607 78-79 0.013698361548778619 80-81 0.01634794792184992 82-83 0.020110360571611165 84-85 0.01724880728869416 86-87 0.009591502670518105 88-89 0.042101927468102956 90-91 0.052594289505465304 92-93 0.033861713847851214 94-95 0.05325668609873313 96-97 0.04371817515567645 98-99 0.03441812698619619 100-101 0.16440683444907414 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1887087.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.349700322599396 #Duplication Level Percentage of deduplicated Percentage of total 1 80.52729178459552 47.79270635206265 2 11.374824790414587 13.501848850663606 3 3.4735559523870583 6.184635144838597 4 1.492223564703616 3.542520855179225 5 0.7958038512626893 2.3615360044005542 6 0.49583981579251213 1.765676668517909 7 0.32806185633257495 1.3629261002429789 8 0.236481752746841 1.1228096925830449 9 0.17339046599885521 0.9261604976245126 >10 0.99452111033344 10.660334420101691 >50 0.06672716317826734 2.6979843325489816 >100 0.03545904090884327 3.7566905526816976 >500 0.003222748437212529 1.307749709906179 >1k 0.002417061327909396 2.226001039893523 >5k 1.7904157984514044E-4 0.7904197787549453 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8146 0.4316706119007762 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6731 0.3566873175428584 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4074 0.21588829767784953 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2476 0.13120751719449078 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2353 0.12468953471673537 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2204 0.1167937673249829 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2081 0.11027578484722751 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1994 0.10566550455808343 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1919 0.10169112499847648 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.2991727461426E-5 4.23933819691408E-4 14-15 0.0 0.0 0.0 1.05983454922852E-4 5.03421410883547E-4 16-17 0.0 0.0 0.0 1.3247931865356498E-4 5.56413138344973E-4 18-19 0.0 0.0 0.0 2.11966909845704E-4 6.88892456998538E-4 20-21 0.0 0.0 0.0 2.9145450103784296E-4 8.74363503113529E-4 22-23 0.0 0.0 0.0 3.70942092229982E-4 9.273552305749549E-4 24-25 0.0 0.0 0.0 4.23933819691408E-4 0.001033338685497807 26-27 0.0 0.0 0.0 8.47867639382816E-4 0.001086330412959233 28-29 1.05983454922852E-4 0.0 0.0 0.00238462773576417 0.0011128262766899458 30-31 1.5897518238427798E-4 0.0 0.0 0.009379535760672401 0.0011658180041513718 32-33 1.5897518238427798E-4 0.0 0.0 0.02254798003483676 0.0012188097316127979 34-35 1.5897518238427798E-4 0.0 0.0 0.04419510070282928 0.0012188097316127979 36-37 1.5897518238427798E-4 0.0 0.0 0.07977904569317684 0.0012188097316127979 38-39 1.5897518238427798E-4 0.0 0.0 0.1334861614753321 0.0012188097316127979 40-41 1.5897518238427798E-4 0.0 0.0 0.19185654927409282 0.0012188097316127979 42-43 1.5897518238427798E-4 0.0 0.0 0.2451927229640181 0.001245305595343511 44-45 1.5897518238427798E-4 0.0 0.0 0.31487684457579324 0.0013247931865356499 46-47 2.9145450103784296E-4 0.0 0.0 0.39754393941561783 0.0014042807777277888 48-49 3.1795036476855597E-4 0.0 0.0 0.4826221578549373 0.001536760096381354 50-51 3.1795036476855597E-4 0.0 0.0 0.5787756473336947 0.001536760096381354 52-53 3.1795036476855597E-4 0.0 0.0 0.666635931464739 0.001642743551304206 54-55 3.70942092229982E-4 0.0 0.0 0.7599808593880409 0.001642743551304206 56-57 4.23933819691408E-4 0.0 0.0 0.8686139006839642 0.001695735278765632 58-59 4.23933819691408E-4 0.0 0.0 0.9848777507343329 0.001748727006227058 60-61 4.23933819691408E-4 0.0 0.0 1.107924541899764 0.001801718733688484 62-63 4.23933819691408E-4 0.0 0.0 1.226837978323204 0.001960693916072762 64-65 4.23933819691408E-4 0.0 0.0 1.3511300750839785 0.001960693916072762 66-67 4.23933819691408E-4 0.0 0.0 1.4962744166008244 0.0019871897798034747 68-69 4.7692554715283395E-4 0.0 0.0 1.6564419128529844 0.002013685643534188 70-71 4.7692554715283395E-4 0.0 0.0 1.822306019807248 0.002040181507264901 72-73 4.7692554715283395E-4 0.0 0.0 1.9936282746900382 0.0022521484171106046 74-75 4.7692554715283395E-4 0.0 0.0 2.1630958191116783 0.0024376194632255957 76-77 4.7692554715283395E-4 0.0 0.0 2.348699344545323 0.0024376194632255957 78-79 4.7692554715283395E-4 0.0 0.0 2.550809793083202 0.0024376194632255957 80-81 5.2991727461426E-4 0.0 0.0 2.7652143223921315 0.0024641153269563086 82-83 5.829090020756859E-4 0.0 0.0 2.9876206025477363 0.0025436029181484477 84-85 5.829090020756859E-4 0.0 0.0 3.211033725525108 0.0025436029181484477 86-87 6.094048658063989E-4 0.0 0.0 3.4497614577388322 0.002623090509340587 88-89 6.359007295371119E-4 0.0 0.0 3.7070362945640554 0.002702578100532726 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4015 0.0 52.79755 1 GTATCAA 6825 0.0 40.9232 1 TATCAAC 8700 0.0 32.059498 2 ATCAACG 8675 0.0 32.042343 3 TCAACGC 8765 0.0 31.71333 4 CAACGCA 8905 0.0 31.21475 5 AACGCAG 9270 0.0 30.088202 6 GTGGTAT 1760 0.0 27.669662 1 TAAGGTG 1175 0.0 27.094105 5 ACGCAGA 10320 0.0 26.810915 7 TGGTATC 1790 0.0 26.279678 2 CGCAGAG 10510 0.0 26.234364 8 GTAAGGT 1220 0.0 24.53684 4 GCAGAGT 11670 0.0 23.38101 9 GTGTAAG 770 0.0 22.941793 1 GAGTACT 7315 0.0 22.6467 12-13 TACGACG 190 3.2787466E-7 22.507475 5 GTACTTT 7485 0.0 22.148525 14-15 AGGTAAG 1160 0.0 22.119415 2 CAGAGTA 10880 0.0 21.93353 10-11 >>END_MODULE