FastQCFastQC Report
Thu 26 May 2016
SRR938257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887087
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79590.42176115886548954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69920.37051815841029057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41560.22023361932968644No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25030.13263829383594927No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23110.12246388216335546No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22480.11912540333328563No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21450.11366725540475876No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21020.11138861112391744No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19050.10094924081401652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37000.053.7976071
GTATCAA65600.042.1473051
TGGTATC15850.037.1657682
GTGGTAT16950.035.5946461
ATCAACG83100.032.9821853
TCAACGC83800.032.7598884
TATCAAC83900.032.727782
CAACGCA84850.032.4664465
AACGCAG87650.031.4292986
ACGCAGA100700.027.1204577
CGCAGAG102500.026.5051788
GCAGAGT111950.024.09819
TAAGGTG12350.023.4596465
GTATAGG3600.022.4335041
GTAAGGT13050.021.837324
GTACATG62100.021.6493821
CAGAGTA106750.021.60130310-11
GAGTACT75950.021.54368812-13
AGGTAAG12050.021.2891042
TACATGG61250.021.1742122