##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938257_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1887087 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49070074670643 34.0 31.0 34.0 31.0 34.0 2 32.7126401697431 34.0 31.0 34.0 31.0 34.0 3 32.76945101100267 34.0 31.0 34.0 31.0 34.0 4 36.22901699815642 37.0 35.0 37.0 35.0 37.0 5 36.18729979062969 37.0 35.0 37.0 35.0 37.0 6 36.22587193913158 37.0 37.0 37.0 35.0 37.0 7 36.192778075414644 37.0 37.0 37.0 35.0 37.0 8 36.18306098235004 37.0 37.0 37.0 35.0 37.0 9 37.893839552707426 39.0 38.0 39.0 35.0 39.0 10-11 37.87923026336358 39.0 38.0 39.0 35.0 39.0 12-13 37.828318991122295 39.0 38.0 39.0 35.0 39.0 14-15 39.146147209959054 40.5 38.5 41.0 36.0 41.0 16-17 39.09516916814116 40.0 38.0 41.0 36.0 41.0 18-19 39.11344866452898 40.0 38.5 41.0 36.0 41.0 20-21 39.03351567786753 40.0 38.5 41.0 35.5 41.0 22-23 38.89208632140436 40.0 38.0 41.0 35.0 41.0 24-25 38.84944043385387 40.0 38.0 41.0 35.0 41.0 26-27 38.807583063208 40.0 38.0 41.0 35.0 41.0 28-29 38.7535659458202 40.0 38.0 41.0 35.0 41.0 30-31 38.555331842146124 40.0 38.0 41.0 34.0 41.0 32-33 38.37926974220054 40.0 38.0 41.0 34.0 41.0 34-35 38.176396212787225 40.0 38.0 41.0 34.0 41.0 36-37 38.041614668534095 40.0 38.0 41.0 33.5 41.0 38-39 38.02741315053307 40.0 38.0 41.0 33.0 41.0 40-41 37.96008636591742 40.0 38.0 41.0 33.0 41.0 42-43 37.79600013141948 40.0 37.0 41.0 33.0 41.0 44-45 37.58548228036121 40.0 37.0 41.0 33.0 41.0 46-47 37.262399401829384 40.0 37.0 41.0 32.0 41.0 48-49 36.910567451315174 40.0 36.0 41.0 31.0 41.0 50-51 36.59930119808997 39.5 36.0 41.0 31.0 41.0 52-53 36.16354094962236 39.0 35.0 41.0 30.0 41.0 54-55 35.918968229869634 39.0 35.0 40.5 29.0 41.0 56-57 35.65362911195933 38.5 35.0 40.0 28.5 41.0 58-59 35.29418013053982 38.0 35.0 40.0 28.0 41.0 60-61 34.917807975996865 37.5 34.0 40.0 27.0 41.0 62-63 34.52885823494094 37.0 34.0 40.0 26.5 41.0 64-65 34.120631693186375 36.5 34.0 39.5 26.0 41.0 66-67 33.687739621967616 36.0 33.0 39.0 26.0 40.5 68-69 33.21296209448743 35.0 33.0 39.0 25.0 40.0 70-71 32.68916271480859 35.0 32.0 38.0 23.5 40.0 72-73 32.27718939296386 35.0 32.0 37.0 22.5 39.5 74-75 31.831295536453805 35.0 31.5 37.0 21.5 39.0 76-77 30.554951626501584 33.5 30.0 35.0 20.0 37.5 78-79 31.28805349196937 34.5 31.0 36.0 20.5 38.0 80-81 31.141550177601776 35.0 31.0 35.5 20.0 37.0 82-83 30.783443476638862 34.0 31.0 35.0 18.5 37.0 84-85 30.406803978830865 34.0 31.0 35.0 17.5 36.0 86-87 29.982634345952253 34.0 30.5 35.0 11.0 36.0 88-89 29.510137847380648 34.0 30.0 35.0 7.0 35.5 90-91 29.155539728692954 34.0 29.0 35.0 2.0 35.0 92-93 28.732563469516773 34.0 29.0 35.0 2.0 35.0 94-95 28.294066463284416 34.0 29.0 35.0 2.0 35.0 96-97 27.689381835601644 33.5 28.0 35.0 2.0 35.0 98-99 26.966165311933153 33.0 26.5 35.0 2.0 35.0 100-101 24.492241216223732 31.0 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14984105795308267 1101 2 0.2010429868263941 1101 3 0.13494348976644233 1101 4 0.04119650102826 1101 5 0.07548390161827001 1101 6 0.07118403204945167 1101 7 0.10660370394301566 1101 8 0.07854553599477043 1101 9 0.06259470498601871 1101 10-11 0.11465961995241969 1101 12-13 0.15743730078253293 1101 14-15 0.16909199868675984 1101 16-17 0.18330334080920352 1101 18-19 0.1425286281490017 1101 20-21 0.1820552268833353 1101 22-23 0.10590610372530307 1101 24-25 0.06991045052549794 1101 26-27 0.19055600831572406 1101 28-29 0.10138442452468155 1101 30-31 0.061079831553016106 1101 32-33 0.04506598767734005 1101 34-35 0.13680517253563096 1101 36-37 0.07573094003596736 1101 38-39 0.09264560336763594 1101 40-41 0.30879140581662057 1101 42-43 0.25705800085278696 1101 44-45 0.39750004906508707 1101 46-47 0.4106796830238437 1101 48-49 0.38506193892467167 1101 50-51 0.2286936426325923 1101 52-53 0.2995536868915849 1101 54-55 0.3601125607400064 1101 56-57 0.46514579319406124 1101 58-59 0.47771456374297117 1101 60-61 0.5645475688398705 1101 62-63 0.505140061232531 1101 64-65 0.3698658468992164 1101 66-67 0.44666470598216534 1101 68-69 0.4359182117876088 1101 70-71 0.42750054483529 1101 72-73 0.4829250170003405 1101 74-75 0.6535377056599145 1101 76-77 0.5988568320345458 1101 78-79 0.4589766484476705 1101 80-81 0.5816477790041894 1101 82-83 0.6462805550096853 1101 84-85 0.6708827803719721 1101 86-87 0.7710048883417393 1101 88-89 0.6716828423077139 1101 90-91 0.6085403183521763 1101 92-93 0.9731352154114568 1101 94-95 0.7837564710854608 1101 96-97 0.6883696270135502 1101 98-99 0.44248544759426167 1101 100-101 0.4615673560235365 1102 1 0.09708047394250485 1102 2 0.08774770946861565 1102 3 0.02971320484866169 1102 4 0.04469891078067434 1102 5 -0.01959795908859263 1102 6 5.319223348436708E-4 1102 7 -0.02764401624220625 1102 8 0.02806771989195056 1102 9 0.09345636866018481 1102 10-11 0.0036707355260361396 1102 12-13 0.0607570595710456 1102 14-15 0.15270213854689274 1102 16-17 0.008957310994425427 1102 18-19 0.04898903851566416 1102 20-21 0.11190884033069182 1102 22-23 0.06979820824240335 1102 24-25 0.06159222736352632 1102 26-27 -0.09622593862246731 1102 28-29 -0.07078293514268097 1102 30-31 -0.07961013726195176 1102 32-33 -0.12751554818544264 1102 34-35 -0.0709975821421196 1102 36-37 -0.04337092515964258 1102 38-39 -0.13386743173915505 1102 40-41 0.023486761136972234 1102 42-43 0.034692633956161956 1102 44-45 0.01398175148428038 1102 46-47 0.040917793730706364 1102 48-49 0.03909266326790117 1102 50-51 0.08093831518976202 1102 52-53 0.08542016416431153 1102 54-55 -0.04850584787840262 1102 56-57 -0.04957997934420888 1102 58-59 -0.16446009015042762 1102 60-61 -0.06678615233759899 1102 62-63 -0.01919666622919891 1102 64-65 0.06693102833940401 1102 66-67 0.01755601906096871 1102 68-69 0.12817200951015195 1102 70-71 0.04756143833368043 1102 72-73 -0.010654144547572741 1102 74-75 0.004927447674660357 1102 76-77 0.018229056094266838 1102 78-79 -0.040266612514340494 1102 80-81 0.07375037657553918 1102 82-83 0.12329340702254044 1102 84-85 0.1177574850591796 1102 86-87 0.13092547873108273 1102 88-89 -0.027391204422581694 1102 90-91 -0.11394243538057935 1102 92-93 0.010851089064825459 1102 94-95 -0.09683010871986752 1102 96-97 -0.22936951728809163 1102 98-99 0.009917909557973559 1102 100-101 0.11549619745237649 1103 1 0.04188944681676077 1103 2 0.07991433066589337 1103 3 0.13608200523616887 1103 4 0.08811429316898511 1103 5 0.11943607614369256 1103 6 0.10430270370315498 1103 7 0.12494906146034879 1103 8 0.06552498794237493 1103 9 0.13611698232995195 1103 10-11 0.05016330545610259 1103 12-13 0.10675184404611571 1103 14-15 0.211796109862199 1103 16-17 0.13405178757676595 1103 18-19 0.14750131942742684 1103 20-21 0.1638213734583971 1103 22-23 0.1580313326021212 1103 24-25 0.03230814063019238 1103 26-27 0.03924495493460256 1103 28-29 0.1335425960140597 1103 30-31 0.06539523554995696 1103 32-33 0.12787831650877735 1103 34-35 0.04949746818970624 1103 36-37 0.1360898658407521 1103 38-39 0.05304013223349813 1103 40-41 0.17210695066082593 1103 42-43 0.30675752666695644 1103 44-45 0.36023616387126367 1103 46-47 0.1993852965702203 1103 48-49 0.11441607592212222 1103 50-51 0.2636511537047497 1103 52-53 0.20878935568139667 1103 54-55 0.08226783703541685 1103 56-57 0.23989383306948753 1103 58-59 0.2834666099408807 1103 60-61 0.38831241649007353 1103 62-63 0.47807586617471287 1103 64-65 0.4332823322902257 1103 66-67 0.34874846316146346 1103 68-69 0.5186503359210093 1103 70-71 0.4322189285345672 1103 72-73 0.2557285820013533 1103 74-75 0.19390218710719864 1103 76-77 0.44066186810709596 1103 78-79 0.3343170293612978 1103 80-81 0.29414719214054585 1103 82-83 0.29476683805011206 1103 84-85 0.37088199788711407 1103 86-87 0.4802530768892943 1103 88-89 0.2604420560669318 1103 90-91 0.19896818107303105 1103 92-93 0.43272574108478423 1103 94-95 0.384182534091142 1103 96-97 0.34372612711527495 1103 98-99 0.2994315656868771 1103 100-101 0.41705570482398713 1104 1 0.1343257237126494 1104 2 0.13608834418251092 1104 3 0.11865448642564758 1104 4 0.02785009724361487 1104 5 0.045541893588612936 1104 6 0.031661182765652995 1104 7 0.05150538888453582 1104 8 0.0723897449784161 1104 9 0.13901349455222345 1104 10-11 0.12293714516221854 1104 12-13 0.09776339549109991 1104 14-15 0.16113680646861184 1104 16-17 0.04965663594736469 1104 18-19 0.02336505779021536 1104 20-21 0.113893860415196 1104 22-23 0.14211402738341405 1104 24-25 0.13618414130117884 1104 26-27 0.16703950920161503 1104 28-29 0.21600124681081923 1104 30-31 0.22681457597302312 1104 32-33 0.2852692672232493 1104 34-35 0.23554342416232288 1104 36-37 0.21455769689061555 1104 38-39 0.1370887504004159 1104 40-41 0.20292354696071158 1104 42-43 0.33241435011351683 1104 44-45 0.35002323116994205 1104 46-47 0.4027730854758076 1104 48-49 0.3847871517972905 1104 50-51 0.41327962303530086 1104 52-53 0.33715652230318227 1104 54-55 0.3036022034328383 1104 56-57 0.2665042414648582 1104 58-59 0.43662270093796707 1104 60-61 0.3706237205335583 1104 62-63 0.3766615218974181 1104 64-65 0.414326474029167 1104 66-67 0.2832429280185451 1104 68-69 0.35048109049391485 1104 70-71 0.2802607920178488 1104 72-73 0.2177203040864626 1104 74-75 0.33991784323527696 1104 76-77 0.34235528469634247 1104 78-79 0.46415337922200806 1104 80-81 0.4068908572353145 1104 82-83 0.4413727380500774 1104 84-85 0.513644062376283 1104 86-87 0.45372818959093664 1104 88-89 0.444252269757623 1104 90-91 0.4561932307461234 1104 92-93 0.5036392085648522 1104 94-95 0.43687110975787036 1104 96-97 0.6632998464656836 1104 98-99 0.6331716323043501 1104 100-101 0.473752731754729 1105 1 0.07187919745326354 1105 2 0.11148764564415359 1105 3 0.0771239861768791 1105 4 0.08835922281137698 1105 5 0.0530884041685411 1105 6 0.06554918205451798 1105 7 -0.04704555762381801 1105 8 0.096457403689719 1105 9 0.042053951256548316 1105 10-11 0.02267858843776338 1105 12-13 0.04368701430990285 1105 14-15 0.16739115072777366 1105 16-17 0.007776897386882808 1105 18-19 0.018396706837044974 1105 20-21 0.05171817517968691 1105 22-23 0.12732626180488893 1105 24-25 0.16966688216912473 1105 26-27 0.10149366654188441 1105 28-29 0.1014283947421859 1105 30-31 0.17461093315353082 1105 32-33 0.21775901693219168 1105 34-35 0.2852852686838361 1105 36-37 0.29855468075558633 1105 38-39 0.05847741328837941 1105 40-41 0.13635020598930225 1105 42-43 0.061778270323593176 1105 44-45 0.07507776975560887 1105 46-47 0.09983223036510935 1105 48-49 0.1189788325487271 1105 50-51 0.10196414741069049 1105 52-53 0.07807370266755953 1105 54-55 0.19734347633318094 1105 56-57 0.12023141519942016 1105 58-59 0.0921550493873653 1105 60-61 0.0646908051544699 1105 62-63 0.08215856070222571 1105 64-65 0.10251160150809113 1105 66-67 0.1382476071637555 1105 68-69 0.19104940292747585 1105 70-71 0.22044412438408045 1105 72-73 0.16383914449798453 1105 74-75 -0.009816315882822835 1105 76-77 0.044904708939256466 1105 78-79 0.24723873226289683 1105 80-81 0.3054288712776909 1105 82-83 0.37160393597298125 1105 84-85 0.4965861706089889 1105 86-87 0.4762365447664543 1105 88-89 0.27713008604129286 1105 90-91 0.14554258881431537 1105 92-93 0.17815434911822337 1105 94-95 0.2573050541785129 1105 96-97 0.5293633272805707 1105 98-99 0.3770004694194746 1105 100-101 0.6363642935645082 1106 1 -0.1328294170455706 1106 2 -0.04981997474514799 1106 3 -0.01261312142894866 1106 4 -0.004811099054990109 1106 5 -0.002119807578402799 1106 6 -0.01991453733894133 1106 7 0.05717992796090243 1106 8 -0.06910106929701243 1106 9 -0.06929278864894428 1106 10-11 0.03914475554570629 1106 12-13 -0.08262669647379539 1106 14-15 -0.2551948226432188 1106 16-17 -0.20047746498811136 1106 18-19 -0.10584820560342934 1106 20-21 -0.03256285780667412 1106 22-23 -0.053121318621251135 1106 24-25 -0.0707549036454509 1106 26-27 -0.08159939283541462 1106 28-29 -0.017549973378820027 1106 30-31 -0.09493033418010555 1106 32-33 -0.27159983904442697 1106 34-35 -0.17484258337158565 1106 36-37 -0.25664327439738344 1106 38-39 -0.23685485412962493 1106 40-41 -0.3064632152984217 1106 42-43 -0.30571173599793866 1106 44-45 -0.11910353446710786 1106 46-47 -0.22615454065108764 1106 48-49 -0.31541349170526445 1106 50-51 -0.32266418829370025 1106 52-53 -0.2609999044291911 1106 54-55 -0.8112204907209062 1106 56-57 -0.5908081180475477 1106 58-59 -0.4557575459411751 1106 60-61 -0.6092343037965549 1106 62-63 -0.4787302150591657 1106 64-65 -0.5539458056018347 1106 66-67 -0.7465984341376597 1106 68-69 -0.7878849976309397 1106 70-71 -0.8082990412573139 1106 72-73 -0.6741609872486904 1106 74-75 -0.6954171847174564 1106 76-77 -0.6995210489219197 1106 78-79 -0.48102003322456 1106 80-81 -0.729319457908705 1106 82-83 -0.9207724099521748 1106 84-85 -0.6066541041174922 1106 86-87 -0.496316520828362 1106 88-89 -0.52337641635037 1106 90-91 -0.724728110148007 1106 92-93 -0.6749062185250239 1106 94-95 -0.7709218147462629 1106 96-97 -0.7676456765643493 1106 98-99 -0.6273630612526482 1106 100-101 -0.63271340461311 1107 1 0.008374610134175953 1107 2 0.04545380972082569 1107 3 -0.007438040422464098 1107 4 0.021448595522159053 1107 5 0.011987003080392356 1107 6 0.0634555509603203 1107 7 0.03206791629246908 1107 8 -3.2524984322890305E-4 1107 9 0.06320140333862412 1107 10-11 0.0805051459926176 1107 12-13 0.18669790919273055 1107 14-15 0.24647184220389562 1107 16-17 0.1589661776400746 1107 18-19 0.23148923555223178 1107 20-21 0.11263294972540194 1107 22-23 0.13856397116799712 1107 24-25 0.1200776072500318 1107 26-27 0.11404656295381699 1107 28-29 0.060463124125917034 1107 30-31 0.0733887593332554 1107 32-33 0.13493223712350755 1107 34-35 0.13857051369646456 1107 36-37 0.18015228574829223 1107 38-39 -0.02096055122212448 1107 40-41 0.0602143777826214 1107 42-43 0.07716879253120368 1107 44-45 0.06396007746158716 1107 46-47 0.15066223729942863 1107 48-49 0.164910365033343 1107 50-51 0.06589457307122615 1107 52-53 0.027851004328248052 1107 54-55 0.17698280281516787 1107 56-57 0.29632757109714447 1107 58-59 0.1607536945280046 1107 60-61 0.14351590472419673 1107 62-63 0.15577180791961354 1107 64-65 0.14816920614520512 1107 66-67 0.055357485013054486 1107 68-69 0.18294325005240353 1107 70-71 0.3078960064295231 1107 72-73 0.35698161364915393 1107 74-75 0.5256753984943288 1107 76-77 0.4666941412140666 1107 78-79 0.4868111381161775 1107 80-81 0.5461603308630529 1107 82-83 0.4212521081574714 1107 84-85 0.4710342032180712 1107 86-87 0.5146886801812691 1107 88-89 0.6428237624752775 1107 90-91 0.5781091093861299 1107 92-93 0.6241766722930748 1107 94-95 0.6865922194642415 1107 96-97 0.8345984051583137 1107 98-99 0.6058402377420826 1107 100-101 0.753697647641534 1108 1 0.07792062184140036 1108 2 0.04857761106498515 1108 3 0.020598519622900824 1108 4 -0.018062748973612486 1108 5 -0.009927552562260189 1108 6 0.057662395180919646 1108 7 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2316 92-93 -0.3242467390515458 2316 94-95 -0.25065076799674557 2316 96-97 -0.11621142762026793 2316 98-99 0.09743061879101589 2316 100-101 0.0408196867532844 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 15.0 5 35.0 6 72.0 7 151.0 8 383.0 9 771.0 10 1265.0 11 2020.0 12 3093.0 13 4556.0 14 6309.0 15 8831.0 16 11468.0 17 15831.0 18 15695.0 19 11994.0 20 9977.0 21 10059.0 22 10226.0 23 10974.0 24 12879.0 25 14924.0 26 17770.0 27 21624.0 28 26427.0 29 32285.0 30 39338.0 31 48584.0 32 61122.0 33 79844.0 34 111896.0 35 171263.0 36 285736.0 37 449732.0 38 353292.0 39 36638.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66889284913732 18.293221245231408 14.68877693503267 23.3491089705986 2 16.297951049914673 19.334011723428837 39.24407203654827 25.123965190108223 3 20.0266459491056 22.73697565297944 29.144828258696766 28.091550139218196 4 13.51140673429471 16.16284781782716 35.46487257874173 34.8608728691364 5 14.3828027006704 36.49211721558147 33.654410210022114 15.470669873726012 6 33.90580296509912 34.975546967362924 16.307144291704624 14.811505775833334 7 29.88579752814788 31.884751471447792 19.383367062567864 18.846083937836465 8 27.33400032431023 34.29368729074836 18.672744480632 19.699567904309404 9 26.24081980669596 16.64747474801002 19.581109216943222 37.5305962283508 10-11 25.80620289366627 25.878907543743345 26.108308731923856 22.206580830666525 12-13 26.698159650296994 23.05439547832188 26.83514326578478 23.41230160559635 14-15 23.153013083127593 24.608775324084156 25.192770656572804 27.04544093621545 16-17 22.840457245267633 27.753594369300565 26.178862779521943 23.22708560590986 18-19 22.833155016170426 26.04633490665772 28.56320349830188 22.557306578869973 20-21 23.14837630697472 25.142560994803105 29.143966335415378 22.565096362806802 22-23 22.982459208292994 25.10196933156765 29.02317169266706 22.8923997674723 24-25 22.946073626800786 25.32788338249895 28.936250042726787 22.789792947973478 26-27 22.35695352198601 25.702889259793803 28.85051336291268 23.089643855307507 28-29 22.61109318224332 26.12531907643898 28.651249253479037 22.612338487838663 30-31 22.6332497211972 25.590427359848107 28.634140512371847 23.142182406582844 32-33 22.30313214398133 25.60954025217824 28.68148534400804 23.405842259832397 34-35 23.09062215526254 25.774185982723875 28.480434831578293 22.654757030435295 36-37 23.18388674190886 25.31084104943383 28.34109911540301 23.164173093254302 38-39 22.654095780527317 25.70557530301597 28.356045783720656 23.28428313273606 40-41 23.182411783848302 25.513359755690363 28.46358834547214 22.840640114989196 42-43 22.796462925291472 25.602401577379936 28.245615290261703 23.35552020706689 44-45 22.8317068369023 25.90613882174198 28.352255910937362 22.90989843041836 46-47 23.292943911156165 26.111415666425465 27.646930864059488 22.948709558358875 48-49 23.014068773723732 26.14911236206916 27.59303095193809 23.243787912269013 50-51 23.12951125199845 26.500579994457063 27.486173133512125 22.883735620032354 52-53 23.545602296025567 26.058284541200273 27.445343007503098 22.950770155271062 54-55 23.04697663647728 26.376446872878674 27.610173775771862 22.96640271487218 56-57 23.114223138625828 26.43124031907379 27.41749585472212 23.037040687578262 58-59 23.063483559581513 26.069439299830904 27.377619579807394 23.489457560780185 60-61 22.72523736319523 26.386939234916035 27.634311507630542 23.25351189425819 62-63 22.823457530044987 26.407923958990764 27.766048942099648 23.002569568864605 64-65 22.54575438228338 26.44801220081533 27.626283260920136 23.37995015598115 66-67 22.50858068546919 26.48314571612226 27.55993761813843 23.448335980270123 68-69 23.035028940176172 26.042237157093023 27.723880646447626 23.19885325628318 70-71 22.780163288708998 26.273881384377084 27.353428856221257 23.59252647069266 72-73 22.798604409865575 26.318606402354526 27.345268130192196 23.5375210575877 74-75 22.736922039100477 26.72155549797121 27.166553529328535 23.374968933599778 76-77 22.867679126611545 26.327535508431776 27.30745853264847 23.497326832308207 78-79 22.927029861368343 26.481873914663183 27.367816110227032 23.223280113741442 80-81 23.041942422368443 26.5359784684013 27.119231916705484 23.302847192524776 82-83 22.914688860314563 26.66509987750959 27.1769206128071 23.24329064936875 84-85 22.834694280946433 26.75739238586508 27.095964426883757 23.311948906304732 86-87 23.051965945306033 26.7509308021591 26.818839880831884 23.378263371702978 88-89 22.85455534473663 26.94003530841607 26.65539001809934 23.550019328747958 90-91 22.79391600526209 26.874156270327344 26.80219333282991 23.529734391580654 92-93 22.919592259371875 26.464748219738198 27.014618336670054 23.60104118421987 94-95 22.638807855705643 26.357184379946446 27.174687759493864 23.829320004854043 96-97 22.960367741489325 25.99732444697156 27.1861146799577 23.856193131581406 98-99 22.655977175403148 26.392688837345602 26.798499486245202 24.152834501006048 100-101 22.797041153905464 26.09458387451135 27.10015489481937 24.008220076763816 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 609.0 1 430.0 2 386.0 3 521.5 4 404.5 5 350.0 6 754.0 7 1679.0 8 2744.5 9 3246.5 10 2650.0 11 1900.0 12 1736.0 13 1844.0 14 2025.0 15 2102.0 16 2224.5 17 2364.5 18 2561.5 19 2847.0 20 3172.0 21 3717.5 22 4463.0 23 5409.5 24 6618.0 25 8094.0 26 9969.5 27 12365.0 28 14592.0 29 16485.5 30 18601.5 31 21759.5 32 27198.5 33 29681.0 34 29100.5 35 30361.5 36 33237.5 37 36433.5 38 39340.0 39 42510.5 40 45759.0 41 50304.0 42 52900.5 43 54699.0 44 58054.0 45 62293.0 46 64911.0 47 67706.0 48 69919.5 49 69528.0 50 69958.0 51 69618.0 52 69972.0 53 72176.0 54 74473.5 55 71509.5 56 66736.5 57 62920.0 58 59237.5 59 53397.5 60 43237.5 61 35695.0 62 30003.0 63 21983.5 64 15591.5 65 12116.5 66 9232.5 67 7089.0 68 5425.0 69 4122.5 70 3026.5 71 2050.5 72 1463.5 73 1210.0 74 841.5 75 583.0 76 445.5 77 275.0 78 175.5 79 111.0 80 61.0 81 31.5 82 21.5 83 13.0 84 7.0 85 6.0 86 5.5 87 3.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014148791232200742 3 0.006411999022832546 4 0.0 5 0.0 6 0.0 7 0.0 8 2.6495863730713E-4 9 4.23933819691408E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.85471046114991E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005166693427489035 26-27 0.021726608259184658 28-29 0.0 30-31 2.6495863730713E-5 32-33 4.7692554715283395E-4 34-35 0.020110360571611165 36-37 0.0036829250585691067 38-39 0.01836163356538411 40-41 0.015950509965889224 42-43 0.008505172257558874 44-45 0.0029410408741091425 46-47 2.6495863730713E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.3846277357641698E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.6495863730713E-5 84-85 0.002331636008302744 86-87 2.6495863730713E-4 88-89 7.948759119213899E-5 90-91 2.3846277357641698E-4 92-93 2.6495863730713E-4 94-95 0.0 96-97 2.6495863730713E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1887087.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.25143767719706 #Duplication Level Percentage of deduplicated Percentage of total 1 78.97567726517136 45.214710649613856 2 12.246998605875286 14.023165548339763 3 3.720317669125824 6.389806055199965 4 1.6063565402562368 3.6786488540735136 5 0.8969014245786281 2.5674448005926296 6 0.5305941233764686 1.822636583182494 7 0.37118130458298415 1.4875464328391392 8 0.2613395573588093 1.196965230457131 9 0.18541574661779583 0.9553786255673731 >10 1.0864549840377877 11.336297193477941 >50 0.07431750486465603 2.8990289151915976 >100 0.03852035647356433 4.054840465193268 >500 0.003240189356600274 1.3017715889219434 >1k 0.0024995746465202116 2.27933256714255 >5k 1.8515367752001567E-4 0.7924264902069125 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7959 0.42176115886548954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6992 0.37051815841029057 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4156 0.22023361932968644 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2503 0.13263829383594927 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2311 0.12246388216335546 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2248 0.11912540333328563 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2145 0.11366725540475876 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2102 0.11138861112391744 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1905 0.10094924081401652 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.05983454922852E-4 2 0.0 0.0 0.0 0.0 1.05983454922852E-4 3 0.0 0.0 0.0 0.0 1.05983454922852E-4 4 0.0 0.0 0.0 0.0 1.05983454922852E-4 5 0.0 0.0 0.0 0.0 1.05983454922852E-4 6 0.0 0.0 0.0 0.0 1.05983454922852E-4 7 0.0 0.0 0.0 0.0 1.05983454922852E-4 8 0.0 0.0 0.0 0.0 1.05983454922852E-4 9 0.0 0.0 0.0 0.0 1.05983454922852E-4 10-11 0.0 0.0 0.0 0.0 1.5897518238427798E-4 12-13 2.6495863730713E-5 0.0 0.0 5.2991727461426E-5 4.50429683422121E-4 14-15 5.2991727461426E-5 0.0 0.0 1.3247931865356498E-4 6.623965932678249E-4 16-17 5.2991727461426E-5 0.0 0.0 1.85471046114991E-4 9.538510943056679E-4 18-19 5.2991727461426E-5 0.0 0.0 2.6495863730712995E-4 0.001006842821767094 20-21 5.2991727461426E-5 0.0 0.0 3.44446228499269E-4 0.001033338685497807 22-23 5.2991727461426E-5 0.0 0.0 4.23933819691408E-4 0.0011658180041513718 24-25 5.2991727461426E-5 0.0 0.0 4.7692554715283395E-4 0.0011658180041513718 26-27 5.2991727461426E-5 0.0 0.0 9.00859366844242E-4 0.001351289050266363 28-29 5.2991727461426E-5 0.0 0.0 0.002437619463225596 0.001430776641458502 30-31 5.2991727461426E-5 0.0 0.0 0.009379535760672401 0.001430776641458502 32-33 5.2991727461426E-5 0.0 0.0 0.022336013124991057 0.001563255960112067 34-35 5.2991727461426E-5 0.0 0.0 0.04419510070282928 0.00158975182384278 36-37 5.2991727461426E-5 0.0 0.0 0.0800969960579454 0.00158975182384278 38-39 1.05983454922852E-4 0.0 0.0 0.13444001256963775 0.00158975182384278 40-41 1.05983454922852E-4 0.0 0.0 0.19331382177928202 0.0016162476875734928 42-43 1.5897518238427798E-4 0.0 0.0 0.24712692101636013 0.0017222311424963449 44-45 1.5897518238427798E-4 0.0 0.0 0.31749993508513386 0.001801718733688484 46-47 1.85471046114991E-4 0.0 0.0 0.40130635206537907 0.001801718733688484 48-49 2.11966909845704E-4 0.0 0.0 0.48686149605185136 0.001801718733688484 50-51 2.11966909845704E-4 0.0 0.0 0.5833594317591081 0.001801718733688484 52-53 2.11966909845704E-4 0.0 0.0 0.671352195208806 0.001960693916072762 54-55 2.11966909845704E-4 0.0 0.0 0.7644056686310701 0.002013685643534188 56-57 2.11966909845704E-4 0.0 0.0 0.8733566602917618 0.002066677370995614 58-59 2.11966909845704E-4 0.0 0.0 0.9906273531638976 0.00211966909845704 60-61 2.11966909845704E-4 0.0 0.0 1.114389532650058 0.002146164962187753 62-63 2.11966909845704E-4 0.0 0.0 1.2338593822118429 0.0022256525533798917 64-65 2.11966909845704E-4 0.0 0.0 1.3586019086560397 0.002278644280841318 66-67 2.11966909845704E-4 0.0 0.0 1.5045676219485378 0.002278644280841318 68-69 2.11966909845704E-4 0.0 0.0 1.6655034982488883 0.002278644280841318 70-71 2.11966909845704E-4 0.0 0.0 1.8324274397523803 0.002358131872033457 72-73 2.11966909845704E-4 0.0 0.0 2.0051539754128984 0.0024641153269563086 74-75 2.11966909845704E-4 0.0 0.0 2.1766617013418035 0.0025436029181484477 76-77 2.11966909845704E-4 0.0 0.0 2.3631660861422925 0.0025436029181484477 78-79 2.11966909845704E-4 0.0 0.0 2.568429542464126 0.002596594645609874 80-81 2.6495863730713E-4 0.0 0.0 2.7865169968316246 0.002623090509340587 82-83 2.6495863730713E-4 0.0 0.0 3.011625855087762 0.002861553282917004 84-85 2.6495863730713E-4 0.0 0.0 3.239119341079664 0.0029145450103784296 86-87 2.6495863730713E-4 0.0 0.0 3.4805761472576515 0.003047024329031995 88-89 2.6495863730713E-4 0.0 0.0 3.742726223009326 0.00317950364768556 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3700 0.0 53.797607 1 GTATCAA 6560 0.0 42.147305 1 TGGTATC 1585 0.0 37.165768 2 GTGGTAT 1695 0.0 35.594646 1 ATCAACG 8310 0.0 32.982185 3 TCAACGC 8380 0.0 32.759888 4 TATCAAC 8390 0.0 32.72778 2 CAACGCA 8485 0.0 32.466446 5 AACGCAG 8765 0.0 31.429298 6 ACGCAGA 10070 0.0 27.120457 7 CGCAGAG 10250 0.0 26.505178 8 GCAGAGT 11195 0.0 24.0981 9 TAAGGTG 1235 0.0 23.459646 5 GTATAGG 360 0.0 22.433504 1 GTAAGGT 1305 0.0 21.83732 4 GTACATG 6210 0.0 21.649382 1 CAGAGTA 10675 0.0 21.601303 10-11 GAGTACT 7595 0.0 21.543688 12-13 AGGTAAG 1205 0.0 21.289104 2 TACATGG 6125 0.0 21.174212 2 >>END_MODULE