FastQCFastQC Report
Thu 26 May 2016
SRR938256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862228
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76710.41192592958542135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64560.3466815019428341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38290.20561392052960217No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24140.12962966940675363No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22400.12028602297892632No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA21820.11717147416965056No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA20310.10906290744205327No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC19580.10514287187175793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38100.056.359011
GTATCAA68350.041.9343341
TATCAAC87150.032.5764732
ATCAACG86550.032.4726333
TCAACGC86950.032.323254
CAACGCA88500.031.5421965
TGGTATC16650.030.8465522
AACGCAG91200.030.7085346
GTGGTAT17800.030.2252711
ACGCAGA102050.027.1996427
CGCAGAG103450.026.73758
GGACCGA6850.025.6832396
GCAGAGT112550.024.1950079
GAGTACT72350.022.92654212-13
GTACTTT74500.022.13552714-15
CAGAGTA107150.021.75463510-11
AGAGTAC100200.021.72214510-11
ACCGAGT6600.021.602588
AGGACCG8850.021.4938325
AGTACTT73200.021.13448112-13
Produced by FastQC (version 0.11.5)