##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938256_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1862228 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.891542818602233 33.0 31.0 34.0 30.0 34.0 2 32.0067322583486 33.0 31.0 34.0 30.0 34.0 3 32.033187128536355 34.0 31.0 34.0 30.0 34.0 4 35.55800632360806 37.0 35.0 37.0 33.0 37.0 5 35.55710793737394 37.0 35.0 37.0 33.0 37.0 6 35.58223858732658 37.0 35.0 37.0 33.0 37.0 7 35.50975229671125 37.0 35.0 37.0 33.0 37.0 8 35.49814201053791 37.0 35.0 37.0 33.0 37.0 9 37.120213529170435 39.0 37.0 39.0 34.0 39.0 10-11 37.02449189895115 39.0 37.0 39.0 33.0 39.0 12-13 36.86687451805042 39.0 37.0 39.0 33.0 39.0 14-15 38.02939892429929 40.0 38.0 41.0 33.0 41.0 16-17 37.84283852460601 40.0 38.0 41.0 33.0 41.0 18-19 38.01035775425995 40.0 38.0 41.0 33.0 41.0 20-21 37.99485992048235 40.0 38.0 41.0 33.0 41.0 22-23 37.87704862132885 40.0 38.0 41.0 33.0 41.0 24-25 37.74842044046164 40.0 38.0 41.0 32.0 41.0 26-27 37.5525875456711 40.0 37.5 41.0 32.0 41.0 28-29 37.35907203629201 40.0 37.0 41.0 31.5 41.0 30-31 37.1243346679354 40.0 37.0 41.0 31.0 41.0 32-33 36.82347435437551 39.5 36.0 41.0 30.0 41.0 34-35 36.56842287840158 39.0 36.0 41.0 30.0 41.0 36-37 36.290791997542726 39.0 35.0 41.0 30.0 41.0 38-39 35.97231085559878 39.0 35.0 40.0 28.5 41.0 40-41 35.845144364707224 39.0 35.0 40.0 28.0 41.0 42-43 35.5966739840664 39.0 35.0 40.0 27.0 41.0 44-45 35.24886802260518 38.5 35.0 40.0 26.0 41.0 46-47 34.85999163367751 38.0 34.0 40.0 24.0 41.0 48-49 34.42154290452082 38.0 34.0 40.0 23.0 41.0 50-51 33.673186634504475 37.5 33.0 39.5 22.0 40.5 52-53 33.733914160886854 38.0 33.0 39.5 22.0 40.5 54-55 33.78778296749915 38.0 33.0 40.0 21.0 41.0 56-57 33.43713202679801 37.5 33.0 40.0 18.5 41.0 58-59 32.995969881239034 37.0 32.5 40.0 16.0 41.0 60-61 32.452723297039896 36.5 31.5 39.5 10.5 41.0 62-63 31.87527977240166 36.0 31.0 39.0 7.5 40.5 64-65 31.301560013059625 35.0 30.5 39.0 4.0 40.0 66-67 30.701566618051064 35.0 29.5 38.0 2.0 40.0 68-69 29.99199292460429 35.0 29.0 37.5 2.0 40.0 70-71 29.381322265587244 34.0 28.0 37.0 2.0 39.0 72-73 28.67253419022805 34.0 26.5 36.0 2.0 39.0 74-75 27.982946771286866 34.0 26.0 36.0 2.0 38.5 76-77 27.232198742581467 33.0 25.0 35.0 2.0 37.5 78-79 26.455686145842506 32.5 23.0 35.0 2.0 37.0 80-81 25.724838741550442 32.0 20.5 35.0 2.0 36.0 82-83 24.981638123795797 31.5 18.0 35.0 2.0 36.0 84-85 24.24947348015388 31.0 10.5 34.0 2.0 35.0 86-87 23.490900147565178 30.5 4.5 34.0 2.0 35.0 88-89 22.600153418378415 30.0 2.0 34.0 2.0 35.0 90-91 21.685507896992206 29.0 2.0 34.0 2.0 35.0 92-93 20.743894141855883 28.5 2.0 34.0 2.0 35.0 94-95 19.65723074725544 26.5 2.0 33.5 2.0 35.0 96-97 18.383482849575884 24.5 2.0 33.0 2.0 35.0 98-99 16.825107344535684 11.0 2.0 32.0 2.0 34.0 100-101 13.059320341010874 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.022572550766092547 1101 2 0.04943532803130779 1101 3 0.048272645425761596 1101 4 -0.10301768943020306 1101 5 0.04196002588383152 1101 6 0.02684735023261453 1101 7 6.350441865023981E-4 1101 8 -0.0017410593827520415 1101 9 -0.029384702201404878 1101 10-11 -0.06875361238112276 1101 12-13 -0.0745058583215652 1101 14-15 0.01684934027984042 1101 16-17 0.07486176664436783 1101 18-19 0.12071446618656978 1101 20-21 0.04149214451651062 1101 22-23 0.019736012954076898 1101 24-25 0.0429873318134284 1101 26-27 0.06691073491423083 1101 28-29 -0.014350265325539624 1101 30-31 -0.058786362258445024 1101 32-33 -0.15409009886267455 1101 34-35 -0.27532389242170296 1101 36-37 -0.16832298412616353 1101 38-39 -0.18352123950600685 1101 40-41 -0.12146257341171207 1101 42-43 -0.1113711966138311 1101 44-45 -0.2174042063907322 1101 46-47 -0.02877797446532071 1101 48-49 0.05541643422237996 1101 50-51 -0.009212263355514949 1101 52-53 0.0032704121449711465 1101 54-55 -0.2875191054501016 1101 56-57 -0.39023509574365534 1101 58-59 -0.24041171310939546 1101 60-61 -0.3463189458086191 1101 62-63 -0.43385773248548887 1101 64-65 -0.3679394123209079 1101 66-67 -0.5228597607716381 1101 68-69 -0.6580181095216417 1101 70-71 -0.651987911633281 1101 72-73 -0.7132658392591757 1101 74-75 -0.7718097665688397 1101 76-77 -0.9145327794029292 1101 78-79 -0.916760870593226 1101 80-81 -0.9507399212413787 1101 82-83 -1.000500825032038 1101 84-85 -1.3250256611229254 1101 86-87 -1.0808542718726564 1101 88-89 -1.0898522584646315 1101 90-91 -1.3583017367019252 1101 92-93 -1.3839106474967586 1101 94-95 -1.6207317361043359 1101 96-97 -1.790041464374717 1101 98-99 -1.6348341613061876 1101 100-101 -1.521491070880959 1102 1 0.15928627854179211 1102 2 0.22420147297743398 1102 3 0.2764717610497982 1102 4 0.2422894659575121 1102 5 0.16572611411412197 1102 6 0.30604886943483933 1102 7 0.2349493007885144 1102 8 0.25649320050487745 1102 9 0.43156996228528755 1102 10-11 0.3337391465706858 1102 12-13 0.4061489553864561 1102 14-15 0.4810778777347835 1102 16-17 0.42801702527837904 1102 18-19 0.3732478062997231 1102 20-21 0.3511385310330084 1102 22-23 0.462376154861019 1102 24-25 0.3776135255351747 1102 26-27 0.2622125120757346 1102 28-29 0.14144853816581104 1102 30-31 0.13804654700990682 1102 32-33 0.23931834710375455 1102 34-35 0.3418958188751233 1102 36-37 0.20445028354717465 1102 38-39 0.1356749210271957 1102 40-41 0.26482529663469734 1102 42-43 0.37078912928711816 1102 44-45 0.1600361881950576 1102 46-47 0.14267440085273364 1102 48-49 0.3316800729150984 1102 50-51 0.36234532962424026 1102 52-53 0.2108282867482032 1102 54-55 0.12303336550258592 1102 56-57 0.31246457823804263 1102 58-59 0.399711614506721 1102 60-61 0.4915185378855753 1102 62-63 0.36921548375245195 1102 64-65 0.23470608825504868 1102 66-67 0.23556889754671673 1102 68-69 0.183092660425217 1102 70-71 -0.1585691003581715 1102 72-73 -0.12026359604991654 1102 74-75 -0.3311863617018389 1102 76-77 -0.5329994568912362 1102 78-79 -0.4569978254611051 1102 80-81 -0.3973707069904471 1102 82-83 -0.4914935673602443 1102 84-85 -0.5061017366214848 1102 86-87 -0.41178611312306757 1102 88-89 -0.2967530116118695 1102 90-91 -0.3747374004043742 1102 92-93 -0.5765262451350353 1102 94-95 -0.9202193378261505 1102 96-97 -0.9776541382136585 1102 98-99 -0.8976526256368125 1102 100-101 -1.172614729013521 1103 1 0.25788131637000333 1103 2 0.28214161757056644 1103 3 0.2628198583775969 1103 4 0.1724929536879003 1103 5 0.19485463797781932 1103 6 0.23361835708814738 1103 7 0.2560950085095399 1103 8 0.22082756735898101 1103 9 0.132384220007971 1103 10-11 0.27196323936531286 1103 12-13 0.2790446082013034 1103 14-15 0.24427509736811714 1103 16-17 0.1375835339674012 1103 18-19 0.3612139055214598 1103 20-21 0.27942413745727634 1103 22-23 0.2307122936788133 1103 24-25 0.3895290085542982 1103 26-27 0.2612285035242152 1103 28-29 0.35529043305948704 1103 30-31 0.27678969496176364 1103 32-33 0.35226569217011416 1103 34-35 0.35277827998901046 1103 36-37 0.0856729684238573 1103 38-39 0.14099305697826736 1103 40-41 0.3618534037352319 1103 42-43 0.17374328758378965 1103 44-45 0.395933531035233 1103 46-47 0.3958231346806613 1103 48-49 0.09671641575565104 1103 50-51 0.24656867936145943 1103 52-53 0.16567538940424242 1103 54-55 0.3009782168742703 1103 56-57 0.12853224061161228 1103 58-59 0.09684500678079644 1103 60-61 0.10594189577921753 1103 62-63 -0.04684783850735741 1103 64-65 -0.22196049485195246 1103 66-67 -0.4561111043689223 1103 68-69 -0.20027950320754684 1103 70-71 -0.5686685076707398 1103 72-73 -0.4142980722674068 1103 74-75 -0.6268783123996435 1103 76-77 -0.5909757067411121 1103 78-79 -0.6645615213074301 1103 80-81 -0.6351936011653798 1103 82-83 -0.8420374472199086 1103 84-85 -0.7289710681429433 1103 86-87 -0.7858094472209096 1103 88-89 -0.7638893708775072 1103 90-91 -0.8546542722598964 1103 92-93 -0.8680818055129365 1103 94-95 -0.9510466794952457 1103 96-97 -1.0053743699906974 1103 98-99 -1.3144333761846596 1103 100-101 -1.134709821654372 1104 1 0.38197408298781 1104 2 0.3292136065351343 1104 3 0.29502884191066414 1104 4 0.21999808344045846 1104 5 0.30634218000730584 1104 6 0.07827772427497592 1104 7 0.32766704058127516 1104 8 0.32268255485789865 1104 9 0.3800393626926848 1104 10-11 0.3151328229501118 1104 12-13 0.34278751707275035 1104 14-15 0.46201473009507055 1104 16-17 0.5821272015249477 1104 18-19 0.46929580913384683 1104 20-21 0.44519291062737665 1104 22-23 0.48325561637904 1104 24-25 0.5278462772843611 1104 26-27 0.32754525496830666 1104 28-29 0.23277366437253022 1104 30-31 0.3447665895216403 1104 32-33 0.33190359198906094 1104 34-35 0.3009401904985438 1104 36-37 0.18065132862732014 1104 38-39 0.06517186549623943 1104 40-41 0.343335083190361 1104 42-43 0.24379329189450516 1104 44-45 0.2795728102834474 1104 46-47 0.13038921796370317 1104 48-49 0.11931819177082303 1104 50-51 0.17173050365665432 1104 52-53 0.3036579741098251 1104 54-55 0.2848071676458872 1104 56-57 0.33557112327391536 1104 58-59 0.32854998134756386 1104 60-61 0.35581895414632214 1104 62-63 0.27918147436443874 1104 64-65 0.12517460300130878 1104 66-67 -0.003523122646356569 1104 68-69 0.05254160206015257 1104 70-71 -0.11753996210646633 1104 72-73 -0.024815185811675633 1104 74-75 -0.4404686219090834 1104 76-77 -0.5006310218544847 1104 78-79 -0.5635540206608205 1104 80-81 -0.8282919717145631 1104 82-83 -0.9147613027246955 1104 84-85 -0.7911581532364877 1104 86-87 -0.843463555333674 1104 88-89 -0.8586706451252866 1104 90-91 -0.8821133635608689 1104 92-93 -0.9910877543016241 1104 94-95 -1.1352139352931552 1104 96-97 -1.3243001394535838 1104 98-99 -1.2059121243526043 1104 100-101 -1.1408646625889354 1105 1 0.04263840073284797 1105 2 -0.07777358116505795 1105 3 0.09944075734948399 1105 4 0.023926334561537033 1105 5 0.13982007879013736 1105 6 0.08098047891943594 1105 7 0.06175032053002383 1105 8 0.10485808336526503 1105 9 0.10361207249409432 1105 10-11 0.20006331622364826 1105 12-13 0.08950052155705635 1105 14-15 0.20259416635388305 1105 16-17 0.1284481496351404 1105 18-19 0.2529197681344826 1105 20-21 0.1039914372128905 1105 22-23 0.06068995334253202 1105 24-25 0.11236709727155159 1105 26-27 0.2486218084596601 1105 28-29 0.22863080722967055 1105 30-31 0.08200489829806656 1105 32-33 0.015132291540631115 1105 34-35 -0.04661426177564465 1105 36-37 0.010195851369161346 1105 38-39 -0.008346712338479279 1105 40-41 0.07566435929542337 1105 42-43 0.06709406063249901 1105 44-45 0.049040779533932266 1105 46-47 0.006055137246214315 1105 48-49 0.0456878266202807 1105 50-51 0.17270551967202863 1105 52-53 0.19951639819694833 1105 54-55 0.26926552767151435 1105 56-57 0.3514961713200435 1105 58-59 0.32576714982931065 1105 60-61 0.20658064536988974 1105 62-63 0.19945149358288816 1105 64-65 -0.014002206736041245 1105 66-67 -0.23157533276807385 1105 68-69 -0.16879600203978384 1105 70-71 -0.14710274237552312 1105 72-73 -0.03760698593979939 1105 74-75 -0.11789431057019684 1105 76-77 -0.43328605068215964 1105 78-79 -0.578386820148328 1105 80-81 -0.5253951107280166 1105 82-83 -0.6169083238649868 1105 84-85 -0.7099765388867105 1105 86-87 -0.5706208263138173 1105 88-89 -0.5988599468549474 1105 90-91 -0.7094615378081492 1105 92-93 -0.9544804512907348 1105 94-95 -0.9585185477210878 1105 96-97 -0.8974391529065002 1105 98-99 -0.9575101384461355 1105 100-101 -1.0753073275472946 1106 1 0.3403116180018202 1106 2 0.27031234603093424 1106 3 0.2897599343476358 1106 4 0.3229294559894811 1106 5 0.2655207514358793 1106 6 0.2066124581685358 1106 7 0.357703455147103 1106 8 0.12002008987190749 1106 9 0.37267311619408616 1106 10-11 0.47283380334226166 1106 12-13 0.5269345758962842 1106 14-15 0.5507391698709796 1106 16-17 0.05397489060057836 1106 18-19 0.3815426578733323 1106 20-21 0.6493812859775119 1106 22-23 0.6008151401885726 1106 24-25 0.5800441064160111 1106 26-27 0.4817287283576661 1106 28-29 0.4155180621316319 1106 30-31 0.3724570657121191 1106 32-33 0.3914869253223898 1106 34-35 0.3736835378374721 1106 36-37 0.45029383563012715 1106 38-39 0.49988825205086584 1106 40-41 0.558605041173557 1106 42-43 0.443175294695628 1106 44-45 0.5902842948772857 1106 46-47 0.891126963061744 1106 48-49 1.0101788360285227 1106 50-51 0.8530985008555376 1106 52-53 0.8778911673871335 1106 54-55 0.9325634701668903 1106 56-57 0.6999667815372206 1106 58-59 0.7030601354091885 1106 60-61 0.9648417132499603 1106 62-63 0.9452735611991763 1106 64-65 0.511517390116154 1106 66-67 0.32517365606512527 1106 68-69 0.4204449634046874 1106 70-71 0.3803031751484376 1106 72-73 0.21544987021073325 1106 74-75 0.2275359299116424 1106 76-77 0.09672472044243463 1106 78-79 0.06808673564169965 1106 80-81 -0.057970893283190605 1106 82-83 -0.2041324511840763 1106 84-85 -0.19450129049139164 1106 86-87 -0.4160318326446024 1106 88-89 -0.48299067313649147 1106 90-91 -0.3526886436729164 1106 92-93 -0.009925639455687474 1106 94-95 -0.44509173641360533 1106 96-97 -0.578613864943776 1106 98-99 -0.6923343932601682 1106 100-101 -0.9127361611883753 1107 1 -0.10250008114592646 1107 2 -0.16444799427540246 1107 3 -0.09635514998396744 1107 4 -0.025955310683144717 1107 5 -0.1430722070240975 1107 6 -0.11255510247274714 1107 7 -0.0809773363184263 1107 8 -0.010354932477461887 1107 9 -0.07008829688177798 1107 10-11 -0.0740384029870782 1107 12-13 -0.13852150015115683 1107 14-15 -0.25141586767595214 1107 16-17 -0.17623754011638226 1107 18-19 -0.11955525469594619 1107 20-21 -0.03061863952460442 1107 22-23 0.039739912225044804 1107 24-25 0.12025154577473529 1107 26-27 0.0113304626684112 1107 28-29 0.013458568121258452 1107 30-31 -0.15090919568605443 1107 32-33 -0.04819772513353371 1107 34-35 0.048670686952441145 1107 36-37 0.006941095597937874 1107 38-39 -0.046935530653712476 1107 40-41 -0.022397439605988723 1107 42-43 0.022790252380829656 1107 44-45 -0.0377179698583987 1107 46-47 -0.08046792489343346 1107 48-49 -0.10074665118764159 1107 50-51 -0.046330286616900196 1107 52-53 -0.28902692963891496 1107 54-55 -0.2257966824047699 1107 56-57 -0.24064164268353494 1107 58-59 -0.3200295525936667 1107 60-61 -0.2537676719935007 1107 62-63 -0.19187425005298664 1107 64-65 -0.3732651133107474 1107 66-67 -0.5045707416939749 1107 68-69 -0.3311828153663967 1107 70-71 -0.3478540451865051 1107 72-73 -0.30577364579141175 1107 74-75 -0.48576548107828543 1107 76-77 -0.677129502097646 1107 78-79 -0.5596715485230312 1107 80-81 -0.6673254063650234 1107 82-83 -0.7100885570306694 1107 84-85 -0.6020456912304084 1107 86-87 -0.7674858451006479 1107 88-89 -0.6464781425923576 1107 90-91 -0.5590394020613765 1107 92-93 -0.5554261534910907 1107 94-95 -0.6146181906123083 1107 96-97 -1.0919191870726301 1107 98-99 -1.00656460662211 1107 100-101 -0.8484938419202432 1108 1 -0.13798727241461606 1108 2 -0.21858237272180858 1108 3 -0.16174575494946453 1108 4 -0.07421772958787898 1108 5 0.04583837948162994 1108 6 -0.04699697914194445 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6684.0 11 8243.0 12 10069.0 13 12047.0 14 13964.0 15 16850.0 16 19540.0 17 24047.0 18 23899.0 19 21568.0 20 22158.0 21 23613.0 22 26013.0 23 28681.0 24 31933.0 25 35156.0 26 39470.0 27 44938.0 28 51956.0 29 58560.0 30 68366.0 31 80929.0 32 97664.0 33 119858.0 34 152462.0 35 198201.0 36 241506.0 37 239315.0 38 110185.0 39 6077.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42651910527462 17.852938724916847 14.322005415244963 23.39853675456357 2 15.979612405766206 19.017443459723378 39.751779374726006 25.251164759784412 3 19.9123753308646 22.820972271368664 28.85053255621606 28.41611984155068 4 13.220329505483694 15.882063232104471 35.59056024104807 35.30704702136377 5 14.40232975778137 36.511894655179276 33.47977709559302 15.60599849144633 6 33.79282885674931 34.82803891184573 16.471838412534435 14.907293818870523 7 29.860969181950196 31.95799494433389 19.408594632388535 18.772441241327382 8 27.319364930611158 34.13127189055983 18.759652265871093 19.789710912957922 9 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28.534830488085174 23.444083178649148 34-35 23.17975511941563 25.833797132498397 28.318882775668026 22.667564972417946 36-37 23.247366138344717 25.327099739825044 28.252173543845206 23.17336057798504 38-39 22.68177128230117 25.74380301821897 28.216444213665554 23.357981485814307 40-41 23.263043677409946 25.582674857529863 28.272098419308833 22.882183045751365 42-43 22.89622822724397 25.631478767575032 28.09936449813352 23.372928507047476 44-45 22.81750897460519 25.934346045734564 28.26598406394834 22.982160915711905 46-47 23.35640050104671 26.11898059612524 27.53344631866861 22.991172584159433 48-49 23.104616630959622 26.126544768470065 27.445252180565195 23.323586420005114 50-51 23.240912791976818 26.523104150341446 27.323853536575964 22.91212952110577 52-53 23.555347623527602 26.106403247105696 27.337308332431387 23.000940796935307 54-55 23.078875820568193 26.399912103805622 27.50541090799294 23.01580116763325 56-57 23.1491940616938 26.488403608876197 27.29737670559256 23.065025623837446 58-59 23.143575992568664 26.042426101658673 27.317068060239393 23.496929845533273 60-61 22.838923721094886 26.33419525248806 27.56363200752494 23.263249018892118 62-63 22.96470887840086 26.369309648141826 27.662723761205687 23.003257712251628 64-65 22.677338401673282 26.43451578184256 27.51961398674879 23.368531829735364 66-67 22.671675473980685 26.419524679012486 27.43084821498119 23.47795163202564 68-69 23.220604691213076 25.9833452666732 27.678909422592028 23.117140619521688 70-71 22.93930062626274 26.275124182047886 27.20488782044825 23.580687371241126 72-73 22.955470872568462 26.33882065479302 27.160495153710723 23.545213318927793 74-75 22.915205014509976 26.69282151064907 27.064300518190382 23.327672956650574 76-77 23.159962854170395 26.30650895264981 27.09448822703858 23.439039966141223 78-79 23.23331471355985 26.490020517558087 27.064324155933438 23.21234061294862 80-81 23.407823779649366 26.49705117738054 26.775658516672397 23.319466526297695 82-83 23.273389081022493 26.607642650473373 26.870581322465238 23.24838694603889 84-85 23.337932656880078 26.635964525949205 26.75573671173252 23.270366105438196 86-87 23.565081336228058 26.6497939066849 26.44501653795458 23.340108219132464 88-89 23.398793427930087 26.76878602622434 26.291020024905194 23.54140052094038 90-91 23.396266376531756 26.76395109563916 26.305015793287478 23.534766734541606 92-93 23.542107228938324 26.302259530252016 26.57335479381014 23.58227844699952 94-95 23.359508190334424 26.153102090002996 26.6796802054939 23.807709514168682 96-97 23.805922350025632 25.77170129466776 26.581580618779615 23.840795736527 98-99 23.488908994980758 26.11643433026835 26.1362598442542 24.25839683049669 100-101 24.648819541150726 24.88932022130579 26.1416389317533 24.32022130579019 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 647.0 1 448.0 2 372.0 3 503.0 4 424.5 5 383.5 6 684.0 7 1511.5 8 2445.0 9 2821.5 10 2415.0 11 1793.5 12 1592.5 13 1675.0 14 1833.0 15 1997.0 16 2157.5 17 2294.5 18 2558.5 19 2888.5 20 3264.0 21 3833.0 22 4557.5 23 5459.5 24 6664.5 25 8253.5 26 10242.5 27 12520.5 28 14627.5 29 16590.5 30 18627.5 31 21652.0 32 26588.5 33 28855.0 34 28671.0 35 30248.5 36 33007.0 37 35766.5 38 38506.5 39 41411.0 40 44767.5 41 48783.0 42 51233.0 43 53506.5 44 56207.5 45 59968.5 46 63126.5 47 65464.0 48 67372.0 49 67288.5 50 67720.5 51 67646.5 52 67753.5 53 69948.5 54 71798.5 55 69782.0 56 65740.0 57 61798.0 58 58503.0 59 52989.0 60 43913.5 61 36633.0 62 30801.0 63 23687.0 64 17678.0 65 13962.0 66 10752.5 67 8152.0 68 6369.0 69 4925.0 70 3749.5 71 2695.5 72 1901.5 73 1460.5 74 1087.0 75 790.0 76 588.0 77 403.0 78 272.0 79 168.0 80 108.5 81 77.0 82 51.5 83 35.5 84 25.0 85 21.0 86 14.5 87 10.0 88 9.0 89 8.0 90 3.5 91 1.0 92 3.5 93 3.0 94 1.5 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14300074963967893 2 0.04832920566117575 3 0.14530981168793508 4 0.12619292589307002 5 0.18762471620016452 6 0.19696836262799183 7 0.1572310157510251 8 0.0679830826300539 9 0.11706407593484794 10-11 0.09681950867455542 12-13 0.14493391786612597 14-15 0.13196558101371048 16-17 0.1279918463260138 18-19 0.12691786397798765 20-21 0.1297102180828556 22-23 0.18722197281965475 24-25 0.20926546051289102 26-27 0.1439404841942018 28-29 0.12176274870746223 30-31 0.09636306617664432 32-33 0.12111835929864657 34-35 0.09067095973210584 36-37 0.08360952579383406 38-39 0.048248656985073794 40-41 0.05630352459526975 42-43 0.05909587870013769 44-45 0.03895870967464778 46-47 0.04964483403750775 48-49 0.042100108042624214 50-51 0.052168692555369156 52-53 0.0273060011985643 54-55 0.05055771903332997 56-57 0.0260440719396336 58-59 0.04945688712660319 60-61 0.03675704586119423 62-63 0.05114840932474434 64-65 0.03960309908346346 66-67 0.03796527600272362 68-69 0.06419729485326178 70-71 0.05093361285513911 72-73 0.0487050994829849 74-75 0.0402743380509798 76-77 0.020244567260292508 78-79 0.021909239899733007 80-81 0.02513118694381139 82-83 0.020781558434305573 84-85 0.0193316822644703 86-87 0.024164602830587877 88-89 0.06285481691822914 90-91 0.10439108422813963 92-93 0.07748782641008513 94-95 0.07225216246345775 96-97 0.06497593205558073 98-99 0.053162126227293334 100-101 0.12662251883228048 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1862228.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.93908281486783 #Duplication Level Percentage of deduplicated Percentage of total 1 80.91719841431282 48.501026569025875 2 11.250434683565004 13.48681472402928 3 3.345037258545457 6.014953957763238 4 1.4428157643783537 3.459242143506839 5 0.7807352125473285 2.3398276280678876 6 0.4905333850367376 1.7641272713504679 7 0.31092419375808233 1.3045557699168193 8 0.2220965589784613 1.0649811233205735 9 0.17005428627970287 0.9173608153508116 >10 0.9656266436305387 10.549588561804669 >50 0.0646235573570919 2.644875296046286 >100 0.03417985445275336 3.730849600610063 >500 0.00358761697348812 1.5403743265449843 >1k 0.0019731893354184658 1.9219600931249687 >5k 1.7938084867440599E-4 0.7594621195372558 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7671 0.41192592958542135 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6456 0.3466815019428341 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3829 0.20561392052960217 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2414 0.12962966940675363 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2240 0.12028602297892632 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2182 0.11717147416965056 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2031 0.10906290744205327 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1958 0.10514287187175793 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.369911740130639E-5 7 0.0 0.0 0.0 0.0 5.369911740130639E-5 8 0.0 0.0 0.0 0.0 5.369911740130639E-5 9 0.0 0.0 0.0 0.0 5.369911740130639E-5 10-11 0.0 0.0 0.0 5.369911740130639E-5 5.369911740130639E-5 12-13 0.0 0.0 0.0 5.369911740130639E-5 4.8329205661175757E-4 14-15 5.369911740130639E-5 0.0 0.0 5.369911740130639E-5 5.906902914143703E-4 16-17 5.369911740130639E-5 0.0 0.0 5.369911740130639E-5 6.443894088156767E-4 18-19 5.369911740130639E-5 0.0 0.0 5.369911740130639E-5 6.443894088156767E-4 20-21 5.369911740130639E-5 0.0 0.0 1.0739823480261278E-4 7.517876436182895E-4 22-23 1.0739823480261278E-4 0.0 0.0 2.147964696052256E-4 8.054867610195959E-4 24-25 1.0739823480261278E-4 0.0 0.0 4.564424979111043E-4 8.054867610195959E-4 26-27 1.0739823480261278E-4 0.0 0.0 8.323363197202491E-4 8.591858784209023E-4 28-29 1.6109735220391918E-4 0.0 0.0 0.003168247926677077 9.128849958222086E-4 30-31 1.6109735220391918E-4 0.0 0.0 0.009612142014833843 9.128849958222086E-4 32-33 1.6109735220391918E-4 0.0 0.0 0.023063770923861096 0.001100831906726781 34-35 1.6109735220391918E-4 0.0 0.0 0.04403327626907124 0.001235079700230047 36-37 2.1479646960522556E-4 0.0 0.0 0.08194485315439355 0.001235079700230047 38-39 2.1479646960522556E-4 0.0 0.0 0.13671795290372607 0.0013424779350326597 40-41 2.1479646960522556E-4 0.0 0.0 0.19221599073797624 0.0013424779350326597 42-43 2.1479646960522556E-4 0.0 0.0 0.2502915862074891 0.0013424779350326597 44-45 2.1479646960522556E-4 0.0 0.0 0.32112072205981224 0.0014767257285359257 46-47 2.9534514570718517E-4 0.0 0.0 0.4065023187278894 0.001637823080739845 48-49 3.2219470440783836E-4 0.0 0.0 0.49620669434677167 0.0017452213155424578 50-51 3.2219470440783836E-4 0.0 0.0 0.5898848046533507 0.0018257699916444173 52-53 3.2219470440783836E-4 0.0 0.0 0.6776291624870854 0.002067416019950296 54-55 3.2219470440783836E-4 0.0 0.0 0.774663467631246 0.0020942655786509493 56-57 3.7589382180914474E-4 0.0 0.0 0.8883713487285123 0.0020942655786509493 58-59 3.7589382180914474E-4 0.0 0.0 1.0095971062619615 0.0020942655786509493 60-61 3.7589382180914474E-4 0.0 0.0 1.134742899365706 0.002147964696052256 62-63 3.7589382180914474E-4 0.0 0.0 1.253820692203103 0.0023627611656574813 64-65 3.7589382180914474E-4 0.0 0.0 1.3792081313351532 0.0023627611656574813 66-67 3.7589382180914474E-4 0.0 0.0 1.5265853590430387 0.0023627611656574813 68-69 3.7589382180914474E-4 0.0 0.0 1.6881660033035697 0.0023627611656574813 70-71 3.7589382180914474E-4 0.0 0.0 1.8580968603200039 0.0023896107243581346 72-73 3.7589382180914474E-4 0.0 0.0 2.031195965263115 0.002577557635262707 74-75 3.7589382180914474E-4 0.0 0.0 2.197609530089763 0.0027923541048679324 76-77 3.7589382180914474E-4 0.0 0.0 2.381394759395735 0.002899752339670545 78-79 3.7589382180914474E-4 0.0 0.0 2.587921564921159 0.002899752339670545 80-81 4.2959293921045113E-4 0.0 0.0 2.8055909373073544 0.002926601898371198 82-83 4.2959293921045113E-4 0.0 0.0 3.028925566579388 0.003007150574473158 84-85 4.2959293921045113E-4 0.0 0.0 3.252850886142835 0.003007150574473158 86-87 4.2959293921045113E-4 0.0 0.0 3.486468896397219 0.0031413983679764237 88-89 4.2959293921045113E-4 0.0 0.0 3.734746765702159 0.0033024957201803432 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3810 0.0 56.35901 1 GTATCAA 6835 0.0 41.934334 1 TATCAAC 8715 0.0 32.576473 2 ATCAACG 8655 0.0 32.472633 3 TCAACGC 8695 0.0 32.32325 4 CAACGCA 8850 0.0 31.542196 5 TGGTATC 1665 0.0 30.846552 2 AACGCAG 9120 0.0 30.708534 6 GTGGTAT 1780 0.0 30.225271 1 ACGCAGA 10205 0.0 27.199642 7 CGCAGAG 10345 0.0 26.7375 8 GGACCGA 685 0.0 25.683239 6 GCAGAGT 11255 0.0 24.195007 9 GAGTACT 7235 0.0 22.926542 12-13 GTACTTT 7450 0.0 22.135527 14-15 CAGAGTA 10715 0.0 21.754635 10-11 AGAGTAC 10020 0.0 21.722145 10-11 ACCGAGT 660 0.0 21.60258 8 AGGACCG 885 0.0 21.493832 5 AGTACTT 7320 0.0 21.134481 12-13 >>END_MODULE