FastQCFastQC Report
Thu 26 May 2016
SRR938256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862228
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74230.3986085484698973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64570.34673520106023537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39990.21474277048782425No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24680.13252942174642418No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22820.12254138590978118No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22580.12125260709214983No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20900.11223115536873036No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA20650.11088867743369771No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA18770.1007932433622521No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18650.10014885395343642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41650.051.6722641
GTATCAA69000.039.7283941
ATCAACG85300.031.5703933
TATCAAC87100.031.140262
TCAACGC86650.031.076864
CAACGCA88950.030.27335
AACGCAG92050.029.2013996
TGGTATC18500.027.7304132
GTGGTAT19350.027.4986481
ACGCAGA105550.025.465817
CGCAGAG106650.025.1140988
GGACCGA6400.024.4875286
ACCGAGT6800.023.0464678
GCAGAGT117250.022.762659
TAAGGTG12550.022.3270285
GAGTACT72400.021.94161212-13
GTACATG67150.021.6495911
TACATGG66300.021.6370322
GTACTTT74050.021.32386414-15
TACGCTT1353.8013558E-421.1076625
Produced by FastQC (version 0.11.5)