##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938256_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1862228 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50644550506168 34.0 31.0 34.0 31.0 34.0 2 32.72327717121642 34.0 31.0 34.0 31.0 34.0 3 32.77797724016608 34.0 31.0 34.0 31.0 34.0 4 36.23330655537345 37.0 35.0 37.0 35.0 37.0 5 36.20266154305488 37.0 35.0 37.0 35.0 37.0 6 36.2347768372079 37.0 37.0 37.0 35.0 37.0 7 36.19341992495011 37.0 37.0 37.0 35.0 37.0 8 36.178620448194316 37.0 37.0 37.0 35.0 37.0 9 37.87036227572563 39.0 38.0 39.0 35.0 39.0 10-11 37.847168015946494 39.0 38.0 39.0 35.0 39.0 12-13 37.7614311996168 39.0 38.0 39.0 35.0 39.0 14-15 39.03660480886336 40.0 38.0 41.0 36.0 41.0 16-17 38.91643987739417 40.0 38.0 41.0 35.0 41.0 18-19 39.16454886297488 40.0 39.0 41.0 36.0 41.0 20-21 39.138129971195795 40.0 39.0 41.0 36.0 41.0 22-23 39.04395138511504 40.0 39.0 41.0 35.5 41.0 24-25 38.93826830012222 40.0 38.0 41.0 35.0 41.0 26-27 38.757897260700624 40.0 38.0 41.0 35.0 41.0 28-29 38.63904742061659 40.0 38.0 41.0 34.5 41.0 30-31 38.39126949009466 40.0 38.0 41.0 34.0 41.0 32-33 38.17091623582075 40.0 38.0 41.0 34.0 41.0 34-35 38.01372522591218 40.0 37.5 41.0 33.0 41.0 36-37 38.00972195670992 40.0 38.0 41.0 33.0 41.0 38-39 37.8543483934298 40.0 37.0 41.0 33.0 41.0 40-41 37.670445294561134 40.0 37.0 41.0 33.0 41.0 42-43 37.42510798892509 40.0 37.0 41.0 32.5 41.0 44-45 37.1419463674695 40.0 36.0 41.0 31.5 41.0 46-47 36.74373841441542 39.5 36.0 41.0 31.0 41.0 48-49 36.333681482611155 39.0 35.0 41.0 30.0 41.0 50-51 35.92596046241384 39.0 35.0 40.5 29.0 41.0 52-53 35.37600658995569 38.0 35.0 40.0 27.5 41.0 54-55 35.03099486206845 38.0 34.0 40.0 26.5 41.0 56-57 34.64624632429542 38.0 34.0 40.0 26.0 41.0 58-59 34.17104081777312 37.0 33.5 40.0 24.5 41.0 60-61 33.65737117044745 37.0 33.0 39.5 23.0 41.0 62-63 33.15982951604207 36.0 32.0 39.0 22.0 40.5 64-65 32.64373105763634 35.5 32.0 39.0 20.5 40.0 66-67 32.0679903320109 35.0 31.0 38.0 18.5 40.0 68-69 31.451701402835745 35.0 30.5 38.0 15.5 40.0 70-71 30.802831876655276 34.5 30.0 37.0 10.0 39.0 72-73 30.205729105136427 34.0 29.0 36.5 7.5 39.0 74-75 29.598974185760284 34.0 29.0 36.0 6.0 38.5 76-77 28.28405651724708 32.5 27.0 34.5 2.0 36.5 78-79 28.958023131431812 33.5 29.0 35.0 2.0 37.0 80-81 28.627225291425113 34.0 28.0 35.0 2.0 37.0 82-83 28.05580841873283 33.0 26.5 35.0 2.0 36.0 84-85 27.445717441688128 33.0 26.0 35.0 2.0 36.0 86-87 26.792320542919555 32.5 25.0 35.0 2.0 35.0 88-89 26.018001018135266 32.0 23.5 34.0 2.0 35.0 90-91 25.44782083611674 32.0 21.0 34.0 2.0 35.0 92-93 24.59949479870349 31.0 18.0 34.0 2.0 35.0 94-95 23.736766926498795 31.0 6.0 34.0 2.0 35.0 96-97 22.650432170496845 30.5 2.0 34.0 2.0 35.0 98-99 21.161356987436555 29.5 2.0 34.0 2.0 35.0 100-101 16.859771467296163 21.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14385667299467997 1101 2 0.042753466685802266 1101 3 0.04361715762200191 1101 4 0.047604528075375185 1101 5 0.03212668112045236 1101 6 0.03145703775680175 1101 7 0.045383113415446985 1101 8 0.054389406780003924 1101 9 0.02579047670734269 1101 10-11 -0.006502619203359927 1101 12-13 0.13136690052385092 1101 14-15 0.10183968455597636 1101 16-17 0.0803258586579787 1101 18-19 0.14429627028441416 1101 20-21 0.13170410479165895 1101 22-23 0.1360879262885888 1101 24-25 0.17099765049417215 1101 26-27 0.19440012612518842 1101 28-29 0.22713377735394857 1101 30-31 0.1704392191362345 1101 32-33 0.1254042949181482 1101 34-35 0.027218568306714985 1101 36-37 0.19095095870090262 1101 38-39 0.08360690331811327 1101 40-41 0.0672358225375902 1101 42-43 -0.10028328287066302 1101 44-45 -0.0027736610806812223 1101 46-47 0.10066663063070536 1101 48-49 -0.009402054646706404 1101 50-51 -0.016739103563608637 1101 52-53 -0.13371433482621597 1101 54-55 -0.34110195256671005 1101 56-57 -0.16311261856613868 1101 58-59 -0.054248285844202826 1101 60-61 -0.3967898622027164 1101 62-63 -0.22951382242101914 1101 64-65 -0.36767084099531644 1101 66-67 -0.516878386373854 1101 68-69 -0.3120431543145834 1101 70-71 -0.4191464045744162 1101 72-73 -0.5221570147957699 1101 74-75 -0.7306976085092245 1101 76-77 -0.4356589280683103 1101 78-79 -0.6326934653940128 1101 80-81 -0.7830966909181925 1101 82-83 -0.8694472005226572 1101 84-85 -0.8502204597409708 1101 86-87 -0.9285040682685164 1101 88-89 -0.7365844971697868 1101 90-91 -1.0889023572858427 1101 92-93 -1.047971526402204 1101 94-95 -1.2413423682260643 1101 96-97 -1.315089256600018 1101 98-99 -1.4000065128268133 1101 100-101 -1.4785227772413485 1102 1 0.14225454830197037 1102 2 0.15870137416466434 1102 3 0.1325518972474029 1102 4 0.12002909111894411 1102 5 0.11895706751177926 1102 6 0.06816470280478626 1102 7 0.07138048101818839 1102 8 0.14683231446421985 1102 9 0.15928822078591054 1102 10-11 0.24386456696736758 1102 12-13 0.2370429826718592 1102 14-15 0.21945445101383143 1102 16-17 0.2635062873906122 1102 18-19 0.2671996910318484 1102 20-21 0.24780866533189538 1102 22-23 0.365928085405848 1102 24-25 0.259604416107166 1102 26-27 0.12762700317502862 1102 28-29 0.19452197380979186 1102 30-31 0.19513388256684294 1102 32-33 0.08471967605836994 1102 34-35 0.22010601083302817 1102 36-37 0.2686845155076867 1102 38-39 0.4262644511207654 1102 40-41 0.3773963674790224 1102 42-43 0.4005379233774704 1102 44-45 0.30462423573951014 1102 46-47 0.3514052077671792 1102 48-49 0.46886502219934556 1102 50-51 0.5646315031589779 1102 52-53 0.676985206166627 1102 54-55 0.5430696343620411 1102 56-57 0.39124814502464034 1102 58-59 0.3745911320557056 1102 60-61 0.3288685793633519 1102 62-63 0.359536304602905 1102 64-65 0.3661085265643962 1102 66-67 0.4284907652940788 1102 68-69 0.44855752510206415 1102 70-71 0.5231718260478395 1102 72-73 0.3861416860203519 1102 74-75 0.5509067065883357 1102 76-77 0.3929661430709537 1102 78-79 0.128735656429658 1102 80-81 0.21511558669215347 1102 82-83 0.2725884287591427 1102 84-85 0.22736622259752082 1102 86-87 0.19791573455593436 1102 88-89 0.20013196861198068 1102 90-91 -0.0965073647077972 1102 92-93 -0.3550915156066381 1102 94-95 -0.2234584948862235 1102 96-97 -0.3489310115836801 1102 98-99 -0.3888276252004488 1102 100-101 -0.8822411850517042 1103 1 0.16450361770301214 1103 2 0.11003784859297383 1103 3 0.11932465225807931 1103 4 0.08439862786043761 1103 5 0.09437630940614383 1103 6 0.08152776342307533 1103 7 0.07243782252639619 1103 8 0.06240625148242884 1103 9 0.15795475824689476 1103 10-11 0.12136710088292091 1103 12-13 0.1728962883232441 1103 14-15 0.13575011901907175 1103 16-17 0.1425692044484208 1103 18-19 0.12691355780756197 1103 20-21 0.24386720598361222 1103 22-23 0.22943038510492642 1103 24-25 0.23183124242516584 1103 26-27 0.20166878275636435 1103 28-29 0.2877552874754272 1103 30-31 0.15966112257999754 1103 32-33 0.19573473513068507 1103 34-35 0.13777068541242699 1103 36-37 0.20633298200706207 1103 38-39 0.12664675527967972 1103 40-41 0.24356147663101524 1103 42-43 0.22158436970354956 1103 44-45 0.09542364353846722 1103 46-47 -0.020900414064485062 1103 48-49 0.030343294878967697 1103 50-51 0.07525280915159271 1103 52-53 0.018225528367693755 1103 54-55 0.04402071791260198 1103 56-57 0.1688824163588336 1103 58-59 0.12494161494948486 1103 60-61 -0.30292252946463094 1103 62-63 -0.23098107063098183 1103 64-65 -0.29119440701576593 1103 66-67 -0.2573001512219619 1103 68-69 -0.26593744466867264 1103 70-71 -0.4824046589164759 1103 72-73 -0.5021514557393623 1103 74-75 -0.45480316204465154 1103 76-77 -0.42326128038683564 1103 78-79 -0.30836954755381996 1103 80-81 -0.557293150256168 1103 82-83 -0.5262398153542946 1103 84-85 -0.6222566926045126 1103 86-87 -0.6620179440159895 1103 88-89 -1.0757408151706578 1103 90-91 -1.1306131759330533 1103 92-93 -0.933644581541575 1103 94-95 -1.0099654927584467 1103 96-97 -1.2202587983015079 1103 98-99 -1.2148985015030895 1103 100-101 -2.0339206781264174 1104 1 0.1549914183754808 1104 2 0.16418113680747837 1104 3 0.16717912247103328 1104 4 0.08291953483517744 1104 5 0.07617715430657057 1104 6 0.13722359927099603 1104 7 0.09073146853036462 1104 8 0.06833262444561683 1104 9 0.22907123380512928 1104 10-11 0.1547213555600493 1104 12-13 0.22792120426426976 1104 14-15 0.25286176657490245 1104 16-17 0.2211126995773114 1104 18-19 0.20769690119924178 1104 20-21 0.26315611019949614 1104 22-23 0.18152280686542355 1104 24-25 0.2280945409853814 1104 26-27 0.36250557903191094 1104 28-29 0.13231809461397148 1104 30-31 0.2843139374778332 1104 32-33 0.23982250131742688 1104 34-35 0.19834159669787965 1104 36-37 0.2301019276058156 1104 38-39 0.15027206780661828 1104 40-41 0.12127007219960007 1104 42-43 0.17030977706625094 1104 44-45 0.14925518073995647 1104 46-47 0.3034282349569821 1104 48-49 0.1376263365204906 1104 50-51 0.11893191941972248 1104 52-53 0.13451279451133047 1104 54-55 0.014782680150723593 1104 56-57 0.13331279828830844 1104 58-59 0.12053675246134077 1104 60-61 -0.23053749627211317 1104 62-63 -0.19987614779280705 1104 64-65 -0.058452274073275134 1104 66-67 -0.06871975636033056 1104 68-69 -0.005011157919810216 1104 70-71 -0.13417119051402793 1104 72-73 -0.3890925713527764 1104 74-75 -0.36513474235329824 1104 76-77 -0.2209360799385216 1104 78-79 -0.2810801260519682 1104 80-81 -0.2953679842935877 1104 82-83 -0.3611804136817369 1104 84-85 -0.4816733664557056 1104 86-87 -0.5668204269886594 1104 88-89 -0.6993109505271526 1104 90-91 -0.9030051062718734 1104 92-93 -0.763848047357591 1104 94-95 -0.6682463790417472 1104 96-97 -0.6823303561944307 1104 98-99 -0.7565941649223511 1104 100-101 -1.6245867700308914 1105 1 0.008161181347936974 1105 2 0.08861291131100302 1105 3 0.04229379255431098 1105 4 0.03239679300302356 1105 5 0.0184820870419955 1105 6 0.013313285369648042 1105 7 0.029003729901283748 1105 8 0.024700337450106247 1105 9 0.03003535936566948 1105 10-11 -0.04977191798255376 1105 12-13 -0.006832799579420623 1105 14-15 -0.0343848701963978 1105 16-17 -0.05014867133876777 1105 18-19 -0.001045251267129288 1105 20-21 0.052699351711346765 1105 22-23 0.07443280045927025 1105 24-25 0.0463203577694955 1105 26-27 -0.0731728322429177 1105 28-29 -0.006279599172060557 1105 30-31 0.04518229109731209 1105 32-33 0.15603902918417845 1105 34-35 0.11729226934362202 1105 36-37 0.03511057590818467 1105 38-39 -0.0766946202138783 1105 40-41 0.041841500771027995 1105 42-43 0.026500360025892178 1105 44-45 -0.021396965320263917 1105 46-47 0.025781213816685522 1105 48-49 0.01861512575815283 1105 50-51 -0.021595627024865394 1105 52-53 -0.22134819203575518 1105 54-55 0.018705165736882634 1105 56-57 -0.06549693905954967 1105 58-59 -0.05875504766678574 1105 60-61 -0.2724119357339916 1105 62-63 -0.4206986974404643 1105 64-65 -0.29200544511748205 1105 66-67 -0.2844410888395146 1105 68-69 -0.3068695820069358 1105 70-71 -0.25391974913029713 1105 72-73 -0.3277889198473325 1105 74-75 -0.3042817634935915 1105 76-77 -0.23853217218707812 1105 78-79 -0.37143566737163525 1105 80-81 -0.4535069977465014 1105 82-83 -0.33055805621857104 1105 84-85 -0.6375199391655038 1105 86-87 -0.7244959554960246 1105 88-89 -0.705318317599545 1105 90-91 -0.7706790332417661 1105 92-93 -0.7061638705478543 1105 94-95 -0.8413485571327115 1105 96-97 -1.1023402857267826 1105 98-99 -0.9883257472413796 1105 100-101 -1.28427350930059 1106 1 0.13638427551833843 1106 2 0.14515032448255027 1106 3 0.22282828213489836 1106 4 0.12129978693602084 1106 5 0.09728989940460764 1106 6 0.122096148290602 1106 7 0.1600514965415698 1106 8 0.1269600754260125 1106 9 -0.01960523678310011 1106 10-11 0.09456939477573911 1106 12-13 -0.03553152682815863 1106 14-15 0.106719609712151 1106 16-17 0.19467047268655335 1106 18-19 0.16908905806198504 1106 20-21 0.19279266482134716 1106 22-23 0.27323149033880867 1106 24-25 0.22981828048118302 1106 26-27 0.17469080312154261 1106 28-29 0.20560380889968854 1106 30-31 0.27247500190360796 1106 32-33 0.3359951203650411 1106 34-35 0.3791528712076868 1106 36-37 0.2049797287262649 1106 38-39 0.059517515985895386 1106 40-41 0.1689861086141633 1106 42-43 0.2529151692231153 1106 44-45 0.3417891443253893 1106 46-47 0.29241738061524103 1106 48-49 0.41203319926558635 1106 50-51 0.2609938942096335 1106 52-53 0.12281671607995293 1106 54-55 0.30331839443643815 1106 56-57 0.3912935405852238 1106 58-59 0.4697307300523761 1106 60-61 0.06830319000239626 1106 62-63 0.2759347765769391 1106 64-65 0.05911810407798157 1106 66-67 4.2905316347940925E-4 1106 68-69 -0.00515856688339511 1106 70-71 -0.19747301124231953 1106 72-73 -0.23464754334157334 1106 74-75 -0.003638243753858461 1106 76-77 0.057379746392012265 1106 78-79 -0.04782382353095471 1106 80-81 0.06721780002013844 1106 82-83 0.051352499483527936 1106 84-85 -0.28587294628763615 1106 86-87 -0.3759937463163894 1106 88-89 -0.47775632867841367 1106 90-91 -0.40003556845674737 1106 92-93 -0.5174562406349033 1106 94-95 -0.8490587039717141 1106 96-97 -0.9325082273428862 1106 98-99 -0.9714464058187104 1106 100-101 -1.1799145011233279 1107 1 -0.027971094289156895 1107 2 -0.04200326035564217 1107 3 -0.01775704774173903 1107 4 -0.02110478127424642 1107 5 -0.02635982644317636 1107 6 -0.06947143618999974 1107 7 -0.052980790841466785 1107 8 0.008433941568213754 1107 9 -0.03226615221310425 1107 10-11 -0.054182393173483945 1107 12-13 -0.1259207410244798 1107 14-15 -0.07116457587697056 1107 16-17 -0.14300888096980913 1107 18-19 -0.08093329130329607 1107 20-21 -0.06396243083393927 1107 22-23 -0.029125622719185174 1107 24-25 -0.02033909629931685 1107 26-27 -0.05253798733080828 1107 28-29 -0.11240530964134621 1107 30-31 -0.1897731952475965 1107 32-33 -0.21034163242117643 1107 34-35 -0.08854391495358982 1107 36-37 -0.07242897411656912 1107 38-39 -0.1814300598529499 1107 40-41 -0.3203672125015089 1107 42-43 -0.17696276599352956 1107 44-45 -0.3486637655619731 1107 46-47 -0.17143903961242302 1107 48-49 -0.2642865307540774 1107 50-51 -0.3351633186755194 1107 52-53 -0.3615946016386218 1107 54-55 -0.5472071881370155 1107 56-57 -0.46847443575425274 1107 58-59 -0.513915121905427 1107 60-61 -0.669921488166743 1107 62-63 -0.6193472724128668 1107 64-65 -0.8535768941340685 1107 66-67 -0.9716295841212244 1107 68-69 -0.8725309147587197 1107 70-71 -0.941496418477552 1107 72-73 -0.9382921660842882 1107 74-75 -0.8986018142884546 1107 76-77 -1.0408550262404468 1107 78-79 -1.0233698930225827 1107 80-81 -1.0652869305955157 1107 82-83 -1.210989937491263 1107 84-85 -1.3362204789177135 1107 86-87 -1.286017995377719 1107 88-89 -1.267679744853396 1107 90-91 -1.365657588541378 1107 92-93 -1.2905552408732959 1107 94-95 -1.2779728228107423 1107 96-97 -1.439134814147824 1107 98-99 -1.7020713685697686 1107 100-101 -2.096908958480739 1108 1 -0.07133265635635411 1108 2 -0.06880771024800225 1108 3 -0.09923218675678669 1108 4 -0.05405652540493122 1108 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48-49 -0.3199925071625884 2316 50-51 -0.12654192579287837 2316 52-53 0.0158232870166799 2316 54-55 0.053599443686970005 2316 56-57 0.0890276958104792 2316 58-59 -0.040108093057163785 2316 60-61 0.19108563006621893 2316 62-63 0.41258454355011764 2316 64-65 0.6229316353791603 2316 66-67 0.6650324614432748 2316 68-69 0.8973124884552277 2316 70-71 0.9670646614284628 2316 72-73 1.109754170770195 2316 74-75 1.3386327706476209 2316 76-77 1.232698058941871 2316 78-79 1.2291584790260401 2316 80-81 1.338871844400206 2316 82-83 1.4665210240668074 2316 84-85 1.5665352600355753 2316 86-87 1.7113759633447785 2316 88-89 1.621579229956172 2316 90-91 1.6657602654014205 2316 92-93 1.9037047210731224 2316 94-95 2.1162748812825143 2316 96-97 2.564505985194373 2316 98-99 2.615803510207659 2316 100-101 3.1057637958914093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 12.0 5 22.0 6 72.0 7 181.0 8 431.0 9 803.0 10 1527.0 11 2549.0 12 3804.0 13 5687.0 14 7795.0 15 10818.0 16 14077.0 17 19297.0 18 20008.0 19 16815.0 20 16044.0 21 16619.0 22 18040.0 23 19518.0 24 21843.0 25 24909.0 26 28462.0 27 33950.0 28 40081.0 29 48521.0 30 57788.0 31 70004.0 32 86621.0 33 111562.0 34 149471.0 35 210499.0 36 292253.0 37 338948.0 38 165675.0 39 7517.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62579273803892 18.288786767766222 14.74878078514161 23.33663970905326 2 16.355705361209218 19.320579086214156 39.126827099069374 25.196888453507256 3 20.005724805485503 22.735909245184935 29.199569082183718 28.058796867145848 4 13.56547832246015 16.14448721879771 35.371359130761185 34.91867532798096 5 14.4223571398653 36.45842618915415 33.613950336321544 15.505266334659002 6 33.88282676404253 35.026474062936316 16.262968531844056 14.827730641177103 7 29.882162656774575 31.95398200435178 19.353967398191845 18.809887940681808 8 27.33322665108676 34.36598526066626 18.668981456620777 19.631806631626205 9 26.229924584959523 16.66723945725228 19.546639831427733 37.55619612636047 10-11 25.808601309828873 25.94574885567181 26.07870787035744 22.166941964141877 12-13 26.691133984745026 23.020177079730985 26.806480679965546 23.482208255558444 14-15 23.122813103443832 24.60246543387813 25.179435600796463 27.095285861881575 16-17 22.90184474895653 27.768620338286766 26.11730084806631 23.212234064690396 18-19 22.873890844730077 26.083164897101753 28.523225942258414 22.51971831590976 20-21 23.13543239603314 25.14834381182111 29.126213331557683 22.59001046058807 22-23 22.984806371722474 25.127293757799794 28.963451306714322 22.924448563763406 24-25 22.948988553886736 25.334201858540123 28.860992379550527 22.855817208022618 26-27 22.383747706938173 25.718814963371432 28.80193075067412 23.095506579016273 28-29 22.601221149149737 26.14241405596965 28.593680731712094 22.662684063168516 30-31 22.64334944445235 25.625513009945642 28.566085347133708 23.165052198468295 32-33 22.365762946320213 25.623205107000864 28.588926812398913 23.422105134280013 34-35 23.195270593946894 25.748609598443007 28.38932532496407 22.666794482646026 36-37 23.183561949848997 25.325445598147585 28.31006177586274 23.180930676140683 38-39 22.6576327908855 25.71470151049095 28.30191668241885 23.325749016204696 40-41 23.217791090653723 25.511219179777793 28.394006950416728 22.87698277915176 42-43 22.88490702215272 25.601880392919206 28.16392648552974 23.349286099398334 44-45 22.85319597095414 25.92218190312408 28.33891133065106 22.885710795270718 46-47 23.36207403453556 26.122024427224304 27.56153859991714 22.95436293832299 48-49 23.05102757812792 26.159980689698877 27.500156399477287 23.28883533269592 50-51 23.180457218683454 26.51026326819449 27.39123395235777 22.918045560764288 52-53 23.546900118926178 26.15156871658809 27.360163730925557 22.941367433560167 54-55 23.08476788116547 26.35736284863473 27.51686953066052 23.04099973953928 56-57 23.106914847622427 26.443415161008442 27.376403187890357 23.073266803478774 58-59 23.118237553634295 26.04229190215805 27.330078953227215 23.509391590980442 60-61 22.776921841797613 26.36796857840818 27.55178263510259 23.30332694469162 62-63 22.836728511764605 26.36396155234347 27.713528865819914 23.085781070072013 64-65 22.574788146688025 26.464000042968188 27.5288591811606 23.432352629183185 66-67 22.591007861515312 26.45637820344128 27.41876666906554 23.533847265977865 68-69 23.096430272117523 25.992854152023444 27.641453868241477 23.269261707617556 70-71 22.84546444236893 26.263681434389536 27.186303822772288 23.704550300469247 72-73 22.89389032989104 26.307615472603953 27.177237894239394 23.621256303265614 74-75 22.771812468027 26.693860780993838 27.04648018378853 23.487846567190633 76-77 22.916130127246788 26.33215968272494 27.229195407052487 23.522514782975794 78-79 22.97441197110567 26.484739219750026 27.280120123571944 23.26072868557236 80-81 23.06460391978477 26.4943014706376 27.05409809961303 23.386996509964604 82-83 22.959043760839222 26.584208237659908 27.1198809401346 23.33686706136627 84-85 22.922778537490856 26.649627728656032 27.033097828247076 23.39449590560604 86-87 23.158347928357124 26.65333799228158 26.751584145359185 23.436729934002113 88-89 22.94437305382226 26.87407097078266 26.480424422843747 23.70113155255133 90-91 22.879487096161682 26.792736906081416 26.64472809591809 23.683047901838815 92-93 22.961733755974443 26.393575625965305 26.854772934246686 23.789917683813567 94-95 22.673834386256626 26.249933537741697 27.016438063351554 24.05979401265012 96-97 23.09446880364006 25.908591432198968 26.937486976005776 24.0594527881552 98-99 22.73356474787013 26.188268534309262 26.561195557635724 24.516971160184884 100-101 23.332660235720216 25.545101657431267 26.52239105754018 24.599847049308337 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 598.0 1 407.5 2 345.5 3 473.5 4 381.0 5 351.0 6 717.0 7 1658.5 8 2598.0 9 2933.5 10 2417.5 11 1730.5 12 1591.5 13 1659.0 14 1818.0 15 1966.5 16 2095.0 17 2263.0 18 2480.0 19 2781.0 20 3106.0 21 3658.0 22 4371.5 23 5159.5 24 6452.5 25 8087.0 26 10047.5 27 12387.5 28 14620.0 29 16452.5 30 18455.0 31 21758.0 32 26788.5 33 29118.0 34 28749.0 35 30101.0 36 33079.5 37 35947.0 38 38426.5 39 41397.0 40 45086.0 41 49428.5 42 51506.5 43 53552.0 44 57076.0 45 61231.0 46 63668.0 47 65948.5 48 68451.5 49 68559.0 50 68907.5 51 68385.5 52 68417.5 53 70628.0 54 72755.5 55 70331.5 56 66408.0 57 62682.0 58 58422.5 59 52613.5 60 43207.5 61 35563.0 62 30027.5 63 22697.0 64 16292.0 65 12628.0 66 9704.5 67 7516.0 68 5762.0 69 4381.5 70 3256.5 71 2217.5 72 1559.5 73 1254.0 74 940.5 75 687.5 76 484.5 77 296.0 78 212.0 79 143.5 80 88.0 81 59.0 82 27.0 83 12.0 84 10.5 85 8.0 86 4.0 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00907515084082078 2 0.013961770524339662 3 0.008377062314603797 4 5.36991174013064E-5 5 0.0011813805828287407 6 0.001503575287236579 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.591858784209024E-4 14-15 0.0 16-17 0.0027118054287659727 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0036246904245881817 26-27 0.01941223094057226 28-29 0.006954035703469179 30-31 0.003034000133173811 32-33 0.0 34-35 0.009451044662629925 36-37 6.443894088156767E-4 38-39 0.0027923541048679324 40-41 0.0020137169025489896 42-43 0.0034367435136836095 44-45 2.68495587006532E-5 46-47 1.34247793503266E-4 48-49 5.101416153124107E-4 50-51 0.004161681598601245 52-53 0.007974318934094 54-55 0.0076789737883868146 56-57 0.01648562904220106 58-59 0.023063770923861096 60-61 0.0037052391006901406 62-63 0.019976071673285977 64-65 0.020701009758203614 66-67 0.013209982880721373 68-69 0.031548231473267505 70-71 0.01648562904220106 72-73 0.005262513505328027 74-75 0.0164050803660991 76-77 0.02005662034938794 78-79 0.025695027676525112 80-81 0.011411062447777608 82-83 0.03455538204774066 84-85 0.022231434604140846 86-87 0.031011240299254443 88-89 0.02177499210622974 90-91 0.02762819590297214 92-93 0.023815558567479387 94-95 0.01444506258095142 96-97 0.01503575287236579 98-99 0.010820372156363238 100-101 0.010202832306248215 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1862228.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.62757593580429 #Duplication Level Percentage of deduplicated Percentage of total 1 79.18422424171835 45.63194895407379 2 12.246480503425204 14.114699703149656 3 3.6411217522438597 6.294870608068254 4 1.5587232858706688 3.593017780776734 5 0.8571498616563101 2.4697734370481577 6 0.5252759155228099 1.816222662542391 7 0.3508687930856171 1.415380261193178 8 0.2583124348127594 1.190873556186783 9 0.18388820751146967 0.9537328477859538 >10 1.0775922853084907 11.330327649472444 >50 0.07277836713425577 2.8764795963717407 >100 0.03762509968628124 4.020171972470269 >500 0.0034451925763511336 1.3992643109297023 >1k 0.0023278328218588737 2.148450502178715 >5k 1.862266257487099E-4 0.7447861577522056 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7423 0.3986085484698973 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6457 0.34673520106023537 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3999 0.21474277048782425 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2468 0.13252942174642418 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2282 0.12254138590978118 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2258 0.12125260709214983 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2090 0.11223115536873036 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2065 0.11088867743369771 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1877 0.1007932433622521 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1865 0.10014885395343642 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.369911740130639E-5 2 0.0 0.0 0.0 0.0 5.369911740130639E-5 3 0.0 0.0 0.0 0.0 5.369911740130639E-5 4 0.0 0.0 0.0 0.0 5.369911740130639E-5 5 0.0 0.0 0.0 0.0 5.369911740130639E-5 6 0.0 0.0 0.0 0.0 1.0739823480261278E-4 7 0.0 0.0 0.0 0.0 1.6109735220391918E-4 8 0.0 0.0 0.0 0.0 1.6109735220391918E-4 9 0.0 0.0 0.0 0.0 1.6109735220391918E-4 10-11 0.0 0.0 0.0 5.369911740130639E-5 1.6109735220391918E-4 12-13 5.369911740130639E-5 0.0 0.0 5.369911740130639E-5 4.8329205661175757E-4 14-15 5.369911740130639E-5 0.0 0.0 8.054867610195959E-5 7.249380849176363E-4 16-17 5.369911740130639E-5 0.0 0.0 1.0739823480261278E-4 8.054867610195959E-4 18-19 5.369911740130639E-5 0.0 0.0 1.0739823480261278E-4 8.054867610195959E-4 20-21 5.369911740130639E-5 0.0 0.0 1.6109735220391918E-4 9.128849958222086E-4 22-23 5.369911740130639E-5 0.0 0.0 2.68495587006532E-4 0.001073982348026128 24-25 5.369911740130639E-5 0.0 0.0 5.101416153124107E-4 0.001073982348026128 26-27 5.369911740130639E-5 0.0 0.0 9.128849958222086E-4 0.0011813805828287407 28-29 5.369911740130639E-5 0.0 0.0 0.003060849691874464 0.0012887788176313534 30-31 1.0739823480261278E-4 0.0 0.0 0.009343646427827312 0.0013156283763320065 32-33 1.34247793503266E-4 0.0 0.0 0.022714726660752603 0.0014498761698352725 34-35 1.6109735220391918E-4 0.0 0.0 0.04344258597765687 0.0016109735220391917 36-37 2.1479646960522556E-4 0.0 0.0 0.08143471153908113 0.0016109735220391917 38-39 2.1479646960522556E-4 0.0 0.0 0.1364226077580189 0.0016915221981411515 40-41 2.1479646960522556E-4 0.0 0.0 0.1924576367662821 0.001772070874243111 42-43 2.1479646960522556E-4 0.0 0.0 0.2508017278228015 0.0018257699916444173 44-45 2.1479646960522556E-4 0.0 0.0 0.32168456279252594 0.0018794691090457238 46-47 2.1479646960522556E-4 0.0 0.0 0.4075494515172149 0.00193316822644703 48-49 2.1479646960522556E-4 0.0 0.0 0.4973612253708998 0.002040566461249643 50-51 2.1479646960522556E-4 0.0 0.0 0.5917374242036958 0.0020942655786509493 52-53 2.1479646960522556E-4 0.0 0.0 0.6800724723288448 0.0022016638134535623 54-55 2.1479646960522556E-4 0.0 0.0 0.777992812910127 0.0022822124895555216 56-57 2.68495587006532E-4 0.0 0.0 0.8925598798858142 0.002309062048256175 58-59 2.68495587006532E-4 0.0 0.0 1.0144031772693785 0.002309062048256175 60-61 2.68495587006532E-4 0.0 0.0 1.1403276075754418 0.002309062048256175 62-63 2.68495587006532E-4 0.0 0.0 1.2597275951172466 0.002470159400460094 64-65 2.68495587006532E-4 0.0 0.0 1.385437228953705 0.0025507080765620536 66-67 3.2219470440783836E-4 0.0 0.0 1.5337810407748138 0.00260440719396336 68-69 3.2219470440783836E-4 0.0 0.0 1.697590198407499 0.0026312567526640134 70-71 3.2219470440783836E-4 0.0 0.0 1.8691588785046729 0.002738654987466626 72-73 3.2219470440783836E-4 0.0 0.0 2.0434930631480137 0.002953451457071852 74-75 3.2219470440783836E-4 0.0 0.0 2.212108291788116 0.0030608496918744644 76-77 3.2219470440783836E-4 0.0 0.0 2.398256282259745 0.0030608496918744644 78-79 3.2219470440783836E-4 0.0 0.0 2.6069310524812215 0.0030876992505751177 80-81 3.7589382180914474E-4 0.0 0.0 2.828601009113814 0.0031950974853777302 82-83 4.2959293921045113E-4 0.0 0.0 3.056768558951965 0.003570991307186875 84-85 4.2959293921045113E-4 0.0 0.0 3.28509720614232 0.0036515399832888346 86-87 4.2959293921045113E-4 0.0 0.0 3.5250785618087583 0.003946885128996019 88-89 4.2959293921045113E-4 0.0 0.0 3.7808474579911806 0.004269079833403858 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4165 0.0 51.672264 1 GTATCAA 6900 0.0 39.728394 1 ATCAACG 8530 0.0 31.570393 3 TATCAAC 8710 0.0 31.14026 2 TCAACGC 8665 0.0 31.07686 4 CAACGCA 8895 0.0 30.2733 5 AACGCAG 9205 0.0 29.201399 6 TGGTATC 1850 0.0 27.730413 2 GTGGTAT 1935 0.0 27.498648 1 ACGCAGA 10555 0.0 25.46581 7 CGCAGAG 10665 0.0 25.114098 8 GGACCGA 640 0.0 24.487528 6 ACCGAGT 680 0.0 23.046467 8 GCAGAGT 11725 0.0 22.76265 9 TAAGGTG 1255 0.0 22.327028 5 GAGTACT 7240 0.0 21.941612 12-13 GTACATG 6715 0.0 21.649591 1 TACATGG 6630 0.0 21.637032 2 GTACTTT 7405 0.0 21.323864 14-15 TACGCTT 135 3.8013558E-4 21.107662 5 >>END_MODULE