FastQCFastQC Report
Thu 26 May 2016
SRR938255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279464
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100000.7815772854882983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84750.6623867494513327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47320.3698423714930627No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21920.17132174097903496No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17940.14021496501660072No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14660.11457923005258452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44700.056.851481
GTATCAA78400.042.144341
TATCAAC98600.033.3481032
ATCAACG99050.033.292543
TCAACGC101150.032.601354
CAACGCA102100.032.3445435
AACGCAG104750.031.6145326
TGGTATC17900.029.7308242
GTGGTAT19000.029.3598841
ACGCAGA119200.027.6570787
CGCAGAG121050.027.1951548
GAGTACT69800.026.0985312-13
GCAGAGT125250.026.0536239
GTACATG87100.025.016131
TACATGG87900.024.0554312
GTACTTT75050.024.02061814-15
ACATGGG87950.023.1773323
CAGAGTA123600.022.84709710-11
ATACCGT2205.624861E-821.5965986
GTGTAGG7550.021.4710881
Produced by FastQC (version 0.11.5)