FastQCFastQC Report
Thu 26 May 2016
SRR938255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279464
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94910.7417950016569438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84120.6574628125527565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49330.3855520749313775No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22780.17804330563423434No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17410.1360726054035127No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.12262947609311399No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG12910.1009016275565393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41100.054.2847251
GTATCAA71050.040.4407771
ATCAACG92300.030.9707263
TATCAAC92850.030.9457952
TCAACGC94200.030.3460524
CAACGCA95300.030.0445145
AACGCAG98050.029.1607766
TACACCG700.0083385527.1783815
TGGTATC18900.026.1768972
GTGGTAT19450.026.1704241
ACGCAGA113500.025.1076227
GTACATG83050.024.9170281
CGCAGAG115000.024.7388328
TACATGG84150.024.1955912
GAGTACT66750.024.01445212-13
GCAGAGT120800.023.4330869
ACACCGT1652.4323526E-623.0286
ACATGGG85800.022.9504743
GTACTTT69600.022.8946214-15
AGTACTT67800.021.64606712-13
Produced by FastQC (version 0.11.5)