##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938255_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1279464 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48344306678422 33.0 31.0 34.0 31.0 34.0 2 32.70932749964047 34.0 31.0 34.0 31.0 34.0 3 32.75861532641794 34.0 31.0 34.0 31.0 34.0 4 36.223437314375396 37.0 35.0 37.0 35.0 37.0 5 36.15468196057099 37.0 35.0 37.0 35.0 37.0 6 36.21315488360751 37.0 37.0 37.0 35.0 37.0 7 36.184447549911525 37.0 37.0 37.0 35.0 37.0 8 36.18859538056561 37.0 37.0 37.0 35.0 37.0 9 37.86675904910181 39.0 38.0 39.0 35.0 39.0 10-11 37.845666622898335 39.0 38.0 39.0 35.0 39.0 12-13 37.76101750420489 39.0 38.0 39.0 35.0 39.0 14-15 38.979942382122516 40.0 38.0 41.0 35.5 41.0 16-17 38.89589859503667 40.0 38.0 41.0 35.5 41.0 18-19 39.1657545659745 40.0 38.5 41.0 36.0 41.0 20-21 39.15560148624736 40.0 39.0 41.0 36.0 41.0 22-23 39.04215202616095 40.0 39.0 41.0 35.0 41.0 24-25 38.92569896456641 40.0 38.0 41.0 35.0 41.0 26-27 38.75982677121044 40.0 38.0 41.0 35.0 41.0 28-29 38.634027608436035 40.0 38.0 41.0 34.5 41.0 30-31 38.37222383748195 40.0 38.0 41.0 34.0 41.0 32-33 38.125763601007925 40.0 37.0 41.0 34.0 41.0 34-35 37.99860918322047 40.0 37.0 41.0 33.0 41.0 36-37 37.93122627912939 40.0 37.0 41.0 33.0 41.0 38-39 37.73961283787586 40.0 37.0 41.0 33.0 41.0 40-41 37.50818780364278 40.0 36.5 41.0 33.0 41.0 42-43 37.21681383766952 40.0 36.0 41.0 32.5 41.0 44-45 36.887998021046315 40.0 36.0 41.0 31.0 41.0 46-47 36.415370811527325 39.0 35.0 41.0 30.5 41.0 48-49 35.920470212526496 39.0 35.0 41.0 29.5 41.0 50-51 35.51093817411041 39.0 35.0 40.0 28.0 41.0 52-53 34.97432870326949 38.0 34.5 40.0 26.0 41.0 54-55 34.594677536843555 38.0 34.0 40.0 25.5 41.0 56-57 34.21701861091832 38.0 34.0 40.0 24.0 41.0 58-59 33.724700734057386 37.0 33.0 40.0 22.5 41.0 60-61 33.25927888553332 36.5 33.0 39.5 21.5 41.0 62-63 32.720803398923294 36.0 32.0 39.0 19.5 40.0 64-65 32.170046597637764 35.0 31.0 39.0 17.5 40.0 66-67 31.63010956150388 35.0 31.0 38.0 12.0 40.0 68-69 31.031604249904646 35.0 30.0 38.0 7.5 40.0 70-71 30.433231415655307 34.5 29.5 37.0 6.0 39.0 72-73 29.789775249635785 34.0 29.0 36.5 2.0 39.0 74-75 29.151750654961766 34.0 28.0 36.0 2.0 38.5 76-77 27.853007587552288 32.0 26.5 34.5 2.0 36.5 78-79 28.518165810057962 33.5 28.0 35.0 2.0 37.0 80-81 28.24733716618834 34.0 27.0 35.0 2.0 37.0 82-83 27.711392817617376 33.0 26.0 35.0 2.0 36.0 84-85 27.076260449688306 33.0 25.5 35.0 2.0 36.0 86-87 26.367520305377877 32.5 24.5 35.0 2.0 35.0 88-89 25.59423281936811 32.0 21.5 34.5 2.0 35.0 90-91 24.95599055542008 31.5 19.0 34.0 2.0 35.0 92-93 24.041434538212876 31.0 10.0 34.0 2.0 35.0 94-95 23.157037243720808 31.0 2.0 34.0 2.0 35.0 96-97 22.11745934234961 30.0 2.0 34.0 2.0 35.0 98-99 20.732762312968557 29.0 2.0 34.0 2.0 35.0 100-101 16.884256219792036 21.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16421180153325565 1101 2 0.1457788021423383 1101 3 0.20833427459787401 1101 4 -0.022975646046916154 1101 5 -0.021930164156806597 1101 6 0.010603848141819583 1101 7 0.0419175063884083 1101 8 0.17191808107725848 1101 9 -0.01739431666569402 1101 10-11 0.12782211365993135 1101 12-13 0.3046393622719137 1101 14-15 0.3897528699896 1101 16-17 0.49085694602288754 1101 18-19 0.33885681807932855 1101 20-21 0.3420782255466577 1101 22-23 0.36474675449164806 1101 24-25 0.37588653570812625 1101 26-27 0.36550772261879416 1101 28-29 0.3534156795506149 1101 30-31 0.3621109386523145 1101 32-33 0.3585488660351288 1101 34-35 0.3998678140064058 1101 36-37 0.41202985866578956 1101 38-39 0.35535998432667526 1101 40-41 0.49074917253763317 1101 42-43 0.5281665263379125 1101 44-45 0.5206205698975737 1101 46-47 0.4438894564014717 1101 48-49 0.6680120609624822 1101 50-51 0.7248880740212655 1101 52-53 0.6452047987471019 1101 54-55 0.9061075960798348 1101 56-57 0.6566544434348245 1101 58-59 1.08601338925601 1101 60-61 0.9658833817685419 1101 62-63 1.0470571434155644 1101 64-65 1.1628363956269752 1101 66-67 1.3857106741358862 1101 68-69 1.5266950531669217 1101 70-71 1.6430667140337611 1101 72-73 1.6678841802478068 1101 74-75 1.8026611980489626 1101 76-77 1.5487812635371903 1101 78-79 1.7292722462041326 1101 80-81 2.0545026865938567 1101 82-83 1.8812178766002319 1101 84-85 2.1383605129823273 1101 86-87 2.3684443518997504 1101 88-89 2.659957849178234 1101 90-91 2.867596349815667 1101 92-93 2.900927141750433 1101 94-95 3.1625702363301755 1101 96-97 3.5420015448596303 1101 98-99 3.7786063860968255 1101 100-101 3.610501748864692 1102 1 0.11554701098081921 1102 2 0.09491592217441536 1102 3 0.12938731202789455 1102 4 0.026530067930352175 1102 5 -0.0717808515467695 1102 6 -0.01166601197754602 1102 7 0.005231816129075639 1102 8 0.18600598354962727 1102 9 0.14150206062645054 1102 10-11 0.1851640001693582 1102 12-13 0.2290536582948164 1102 14-15 0.3201027379662449 1102 16-17 0.34014496148418516 1102 18-19 0.28761649661280586 1102 20-21 0.24177456777326256 1102 22-23 0.2608229051403228 1102 24-25 0.25905662221122583 1102 26-27 0.1802509122348539 1102 28-29 0.19001679044617958 1102 30-31 0.3685112100855079 1102 32-33 0.28785285579469644 1102 34-35 0.3046060441223375 1102 36-37 0.35660845943739616 1102 38-39 0.4379088751762694 1102 40-41 0.40604724174173157 1102 42-43 0.3170196642566623 1102 44-45 0.4498186325268918 1102 46-47 0.5223209881077722 1102 48-49 0.5294549102318271 1102 50-51 0.6707885317401079 1102 52-53 0.820170443453712 1102 54-55 0.9780244063501371 1102 56-57 0.9122756837319415 1102 58-59 0.7105114529405299 1102 60-61 0.7990895484321001 1102 62-63 1.0563819776862786 1102 64-65 0.9838195455020937 1102 66-67 1.1570204935402728 1102 68-69 1.4827052611331304 1102 70-71 1.384179184295025 1102 72-73 1.59890938441119 1102 74-75 1.784864222613507 1102 76-77 1.6848927556973656 1102 78-79 1.7738338824008508 1102 80-81 1.7702230844253108 1102 82-83 1.9329902688244474 1102 84-85 1.9938686020000773 1102 86-87 1.940000360565481 1102 88-89 2.1879096243302456 1102 90-91 2.566429690319037 1102 92-93 2.642372409892179 1102 94-95 2.8763462016672463 1102 96-97 3.1861281898754967 1102 98-99 3.6164644177678227 1102 100-101 3.3910808009426887 1103 1 0.22042939255247518 1103 2 0.2288609030665114 1103 3 0.21903403451104708 1103 4 0.10103632540577934 1103 5 -0.025591762323813327 1103 6 0.04398030209228665 1103 7 0.14030377210642087 1103 8 0.08370535757177322 1103 9 0.20200557589819113 1103 10-11 0.25673996888428263 1103 12-13 0.26207602429763455 1103 14-15 0.3083727544409598 1103 16-17 0.3108877516841062 1103 18-19 0.2801122858101195 1103 20-21 0.26612198931875497 1103 22-23 0.30863605144286765 1103 24-25 0.39185121334497097 1103 26-27 0.34305850810834215 1103 28-29 0.5023078816620696 1103 30-31 0.5207326867913693 1103 32-33 0.4861593991594404 1103 34-35 0.5469429206203102 1103 36-37 0.6127408007050974 1103 38-39 0.6965957188936969 1103 40-41 0.719042692385905 1103 42-43 0.9147912783030065 1103 44-45 0.992857104754016 1103 46-47 0.895269133435356 1103 48-49 1.0547738487871214 1103 50-51 1.1060423544733737 1103 52-53 1.1765613441278973 1103 54-55 1.2529618008881513 1103 56-57 1.3381885872895865 1103 58-59 1.406011306310937 1103 60-61 1.5549155797140983 1103 62-63 1.53534331814177 1103 64-65 1.6978189098512928 1103 66-67 1.979229426122906 1103 68-69 2.2116742785306123 1103 70-71 2.2470248578008203 1103 72-73 2.2131937168473392 1103 74-75 2.141482360770823 1103 76-77 1.8677103008876834 1103 78-79 2.089703963976035 1103 80-81 2.0210111225213687 1103 82-83 2.2362805293952164 1103 84-85 2.6404160508754835 1103 86-87 2.750544179782711 1103 88-89 2.950758905998782 1103 90-91 3.1992231299114806 1103 92-93 3.539876021893388 1103 94-95 3.421176088309519 1103 96-97 3.7595596754712517 1103 98-99 4.234190323301512 1103 100-101 4.259141804994584 1104 1 0.13045091446318224 1104 2 0.13245643093578252 1104 3 0.04918713421952248 1104 4 0.032868075903053295 1104 5 -0.03874517364859287 1104 6 0.06497799846152219 1104 7 0.005255966671484202 1104 8 0.13049294301143277 1104 9 0.003571520605227363 1104 10-11 0.08176232080101897 1104 12-13 0.10114295190654587 1104 14-15 0.3073825718105141 1104 16-17 0.22879360278976435 1104 18-19 0.2388893811384989 1104 20-21 0.2201141727005762 1104 22-23 0.21217842980330914 1104 24-25 0.30119780930095175 1104 26-27 0.3765310494703087 1104 28-29 0.36429389678855983 1104 30-31 0.31729092845999674 1104 32-33 0.3492899489146595 1104 34-35 0.3296116666705018 1104 36-37 0.46773001062673103 1104 38-39 0.6343976881922018 1104 40-41 0.5215551351955838 1104 42-43 0.7241272443572129 1104 44-45 0.6067179141991943 1104 46-47 0.627688295216636 1104 48-49 0.4140790900567666 1104 50-51 0.6705855162430012 1104 52-53 0.6112995718757261 1104 54-55 0.9194653786509761 1104 56-57 0.7745504233211662 1104 58-59 0.9867468663902699 1104 60-61 1.0598497471306842 1104 62-63 1.261124615921723 1104 64-65 1.2798082158788802 1104 66-67 1.281629745311193 1104 68-69 1.5033354846313038 1104 70-71 1.5397490540783174 1104 72-73 1.7278702620419004 1104 74-75 1.6899714585688344 1104 76-77 1.4913178565636152 1104 78-79 1.6128727813776251 1104 80-81 1.8511598537770624 1104 82-83 1.7483367309963995 1104 84-85 1.9754640019255127 1104 86-87 2.472323974814909 1104 88-89 2.8112698402360934 1104 90-91 2.6675329172843227 1104 92-93 2.901015285255763 1104 94-95 3.122686262436048 1104 96-97 3.636985986953583 1104 98-99 4.297000665467859 1104 100-101 4.0024959321631535 1105 1 0.19451514164838102 1105 2 0.15615595808594662 1105 3 0.14681256436077916 1105 4 0.07646613452991602 1105 5 -4.7041014141058213E-4 1105 6 0.020899213693752472 1105 7 0.037710413116691655 1105 8 0.10543271644547048 1105 9 0.20400716169563538 1105 10-11 0.13253528498012201 1105 12-13 0.2094787975092558 1105 14-15 0.4240102643521979 1105 16-17 0.21574473982930442 1105 18-19 0.2510216052709495 1105 20-21 0.1555271218875589 1105 22-23 0.31661822089113656 1105 24-25 0.19326392679911208 1105 26-27 0.2987335356664076 1105 28-29 0.4118932536476052 1105 30-31 0.41433921563547216 1105 32-33 0.5040769763703636 1105 34-35 0.5306353145942779 1105 36-37 0.5706929947138804 1105 38-39 0.3984609647889883 1105 40-41 0.5101283834648314 1105 42-43 0.573994705172602 1105 44-45 0.5800566251469874 1105 46-47 0.5573250618037235 1105 48-49 0.6588949882406041 1105 50-51 0.8476298924261485 1105 52-53 0.8993658849235047 1105 54-55 0.9737405821447993 1105 56-57 0.9719564033370816 1105 58-59 1.1635583484119252 1105 60-61 1.015425355575168 1105 62-63 1.0988815803398353 1105 64-65 1.041661374412591 1105 66-67 1.3303820301183436 1105 68-69 1.6436348656624453 1105 70-71 1.5957407486515471 1105 72-73 1.6686995282148018 1105 74-75 1.64414630708578 1105 76-77 1.6372426840318184 1105 78-79 1.578724250579917 1105 80-81 1.757841268143988 1105 82-83 1.8758887213927409 1105 84-85 2.158483505283808 1105 86-87 2.35760968095272 1105 88-89 2.601337141895822 1105 90-91 2.8980811623623133 1105 92-93 3.205156727379862 1105 94-95 3.340339096683305 1105 96-97 3.4965542025361707 1105 98-99 3.5619717540002327 1105 100-101 3.1345890192126085 1106 1 0.1565474913211773 1106 2 0.12911301039692802 1106 3 0.017139919589823194 1106 4 0.07758124133322042 1106 5 -0.007926320336082426 1106 6 0.0710018498216698 1106 7 0.07660667602980453 1106 8 0.1698452467990137 1106 9 0.12535419013536142 1106 10-11 0.12854173639387767 1106 12-13 0.05085131777925511 1106 14-15 0.07437219175063348 1106 16-17 0.01601457766936676 1106 18-19 0.04199567099922774 1106 20-21 0.01757284769732337 1106 22-23 -0.1407273361735264 1106 24-25 -0.12385791437469607 1106 26-27 -0.3135700666301773 1106 28-29 -0.19237952179792472 1106 30-31 0.02080233351010463 1106 32-33 0.11039604353915422 1106 34-35 0.060197863468623325 1106 36-37 0.1664011839965127 1106 38-39 0.00594851469227109 1106 40-41 0.2460056265401036 1106 42-43 0.2653770003119149 1106 44-45 0.34086107116912245 1106 46-47 0.66465505624749 1106 48-49 0.7388321975668575 1106 50-51 0.8381714201867752 1106 52-53 0.7696707322297485 1106 54-55 0.9218488876969246 1106 56-57 1.010025660858588 1106 58-59 1.171232906999407 1106 60-61 1.0469255150598826 1106 62-63 0.8843064784754091 1106 64-65 0.8352939473639793 1106 66-67 1.072322264509424 1106 68-69 1.201736174466003 1106 70-71 1.0675376783136379 1106 72-73 1.3461224096404933 1106 74-75 1.4055854765305682 1106 76-77 1.417628379796728 1106 78-79 1.7391092775379775 1106 80-81 1.5245479346048683 1106 82-83 1.8708373004354186 1106 84-85 2.0883044451117705 1106 86-87 2.0238358354162322 1106 88-89 2.2579967860905796 1106 90-91 2.197886050825911 1106 92-93 1.9342481532760623 1106 94-95 2.555427379187318 1106 96-97 3.0828425045899195 1106 98-99 3.099086032511984 1106 100-101 3.23063354117005 1107 1 0.21365141799032017 1107 2 0.23465772735468704 1107 3 0.21336886174267988 1107 4 0.1652074335789777 1107 5 0.06040716783436295 1107 6 0.01843852144312308 1107 7 0.15519425189999225 1107 8 0.15392412082353957 1107 9 0.2928298807088723 1107 10-11 0.21866080613251881 1107 12-13 0.21023096289258802 1107 14-15 0.41571447466900935 1107 16-17 0.34016219937133485 1107 18-19 0.3446035150472895 1107 20-21 0.20209223330367365 1107 22-23 0.260285298534761 1107 24-25 0.4513445351484009 1107 26-27 0.39479616958932695 1107 28-29 0.2848923257184879 1107 30-31 0.36866132335278934 1107 32-33 0.39427633896836056 1107 34-35 0.39634536130090225 1107 36-37 0.5591087431442858 1107 38-39 0.522221895096088 1107 40-41 0.5517953732265966 1107 42-43 0.6330203020584833 1107 44-45 0.7330615387771999 1107 46-47 0.5086973112951725 1107 48-49 0.7269116754561153 1107 50-51 0.8952211538669488 1107 52-53 0.8435841628007879 1107 54-55 0.7636131017437506 1107 56-57 0.7907801821022389 1107 58-59 0.9154458699623689 1107 60-61 1.0712833281405167 1107 62-63 1.129138789883804 1107 64-65 1.1129923711221892 1107 66-67 1.405355600939501 1107 68-69 1.759962670192504 1107 70-71 1.7856722007959895 1107 72-73 1.8278400177167988 1107 74-75 1.8639110680083313 1107 76-77 1.7581422841693275 1107 78-79 1.7511710885128338 1107 80-81 1.8237029110696241 1107 82-83 2.2761369876644544 1107 84-85 2.352659780798625 1107 86-87 2.485306555718477 1107 88-89 2.7329615606477446 1107 90-91 2.770614977495054 1107 92-93 3.1320390147897506 1107 94-95 3.158889536997304 1107 96-97 3.2530955858484383 1107 98-99 3.5264345243985176 1107 100-101 3.2906058253271198 1108 1 0.18156988988766898 1108 2 0.1657160011388541 1108 3 0.06386092944519106 1108 4 0.012725392107569178 1108 5 0.014262926218805205 1108 6 0.10857144536755214 1108 7 0.048579136459004246 1108 8 0.08171355389402635 1108 9 0.06548468845013389 1108 10-11 0.07650106699672676 1108 12-13 0.14107287693279602 1108 14-15 0.23196610574014187 1108 16-17 0.28254285319307115 1108 18-19 0.273497521547462 1108 20-21 0.31665237327435847 1108 22-23 0.32400558758602216 1108 24-25 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-0.1566293048888454 2316 54-55 0.13154705929327548 2316 56-57 0.0950976250579103 2316 58-59 0.09870932209374672 2316 60-61 0.38362637045583625 2316 62-63 0.3923719531763723 2316 64-65 0.6119132461144616 2316 66-67 0.7324480819526542 2316 68-69 1.221232821280477 2316 70-71 1.2911042332074558 2316 72-73 1.3589444864467914 2316 74-75 1.7597417166253209 2316 76-77 1.943720354672859 2316 78-79 1.7076147744266876 2316 80-81 1.538363924378487 2316 82-83 1.844491699197576 2316 84-85 2.201492963787846 2316 86-87 2.5277528378185146 2316 88-89 2.8283279235878673 2316 90-91 2.9991031966978206 2316 92-93 2.9638343330635273 2316 94-95 3.4085503460057964 2316 96-97 3.715536011337999 2316 98-99 4.053747387882918 2316 100-101 4.497079894372263 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 8.0 5 27.0 6 61.0 7 172.0 8 365.0 9 655.0 10 1091.0 11 1907.0 12 2837.0 13 4294.0 14 5895.0 15 8243.0 16 11480.0 17 16810.0 18 17494.0 19 12460.0 20 11319.0 21 12184.0 22 12533.0 23 14141.0 24 15823.0 25 18036.0 26 20539.0 27 23643.0 28 28346.0 29 34903.0 30 41105.0 31 49621.0 32 60785.0 33 75963.0 34 100273.0 35 139610.0 36 185276.0 37 225534.0 38 120907.0 39 5120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.228442535311665 19.017338510501272 15.039110127365834 21.71510882682123 2 16.22864718541118 20.356434590992908 39.44324976295278 23.971668460643127 3 19.51525667479522 25.180313262052145 29.554961545676235 25.749468517476398 4 13.09627276763104 17.204919621586807 36.05633054254647 33.64247706823568 5 13.873324965333317 37.63643648406272 32.8958653524107 15.594373198193262 6 31.697570232534872 35.73144691839708 17.181647940074907 15.38933490899314 7 28.86216415624043 33.7098972694816 19.49378802373494 17.93415055054304 8 27.57252657757714 33.8994045934932 18.844717545343276 19.683351283586383 9 27.49932784353448 16.560684786754454 19.76351034495695 36.176477024754114 10-11 25.854771998274277 26.732600526470456 25.580008503560865 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22.470284152902963 25.83660817287401 29.680167759321957 22.012939914901068 38-39 21.99509291262758 25.9899763478076 29.674708099429875 22.34022264013494 40-41 22.159933184324267 25.83237222615871 29.92999127284722 22.0777033166698 42-43 22.31598586523829 25.85565566332317 29.446638617038158 22.381719854400387 44-45 22.12308038617378 26.113547971587213 29.470761842829273 22.292609799409735 46-47 22.318134781439728 26.42454183939525 28.84184314681773 22.415480232347296 48-49 22.483438377320503 26.418875404075457 28.696508850581182 22.40117736802286 50-51 22.403600257607874 26.6415076938468 28.352380371780683 22.602511676764646 52-53 22.691181619803295 26.693560741060317 28.169882075619167 22.445375563517224 54-55 22.343379727761 26.54822644482377 28.39485909724697 22.71353473016826 56-57 22.3751508444161 26.920608942494667 28.15522750151626 22.54901271157297 58-59 22.54748863586627 26.45689913901446 28.230923261615803 22.764688963503467 60-61 22.464328812690315 26.58519505042737 28.513307134862725 22.437169002019598 62-63 22.25607754497196 26.597583050402356 28.462934478813 22.683404925812685 64-65 22.12828965879462 26.426652098066068 28.409865381128345 23.035192862010966 66-67 22.139396708073345 26.78739956239471 28.109477136315338 22.96372659321661 68-69 22.110030399650476 26.580486777383033 28.353780182272846 22.95570264069364 70-71 22.163148005727397 26.68312668429905 28.171796939968612 22.98192837000494 72-73 22.27347634379566 26.77477708430135 27.815877514286264 23.135869057616727 74-75 22.166157078276527 27.017055579523923 27.635791237580737 23.18099610461881 76-77 22.196951223324767 26.753195087943077 28.01942844816267 23.03042524056949 78-79 22.285074062263575 26.8400674032251 27.91102367866544 22.963834855845885 80-81 22.063535980691913 27.422655111828075 27.742867325692632 22.770941581787373 82-83 22.34649948962126 27.160824797059966 27.5984765233594 22.894199189959373 84-85 22.313422365828906 26.918241500741935 27.518435104017797 23.24990102941136 86-87 22.32698626582338 26.73138789279026 27.704629227253257 23.2369966141331 88-89 22.216138945683504 27.033507781383452 27.38666347783134 23.3636897951017 90-91 22.202109138231208 27.103813604859965 27.672346919434982 23.02173033747384 92-93 22.307700724678458 26.694459554938632 27.524846341905672 23.472993378477234 94-95 22.0611130911069 26.746629838744973 27.550794707783883 23.64146236236424 96-97 22.18676031008309 26.65382795210649 27.432240153783205 23.72717158402721 98-99 22.03922892711323 26.639670987225898 27.210144247903813 24.11095583775706 100-101 22.352875891779682 26.218713461261906 27.26317426672419 24.165236380234223 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1566.0 1 996.0 2 778.0 3 1067.5 4 758.0 5 649.0 6 1287.5 7 2584.0 8 3974.0 9 4481.0 10 3607.5 11 2515.5 12 2281.5 13 2427.5 14 2531.0 15 2576.5 16 2650.5 17 2727.5 18 2775.5 19 2864.5 20 3004.0 21 3326.5 22 3778.5 23 4289.0 24 4831.5 25 5606.0 26 6749.0 27 8062.5 28 9361.0 29 10942.0 30 12811.5 31 14858.0 32 17399.5 33 19640.0 34 21648.5 35 23494.5 36 25893.0 37 28547.0 38 29951.0 39 30735.0 40 31824.0 41 34611.5 42 37422.0 43 39537.0 44 41400.5 45 43566.0 46 44652.0 47 45118.5 48 47513.0 49 48404.0 50 47594.0 51 45982.0 52 44368.5 53 44749.5 54 45351.5 55 43072.0 56 39429.0 57 36295.0 58 33264.0 59 29523.0 60 25329.0 61 21318.0 62 17593.5 63 14008.5 64 11250.5 65 9202.5 66 6977.0 67 5690.0 68 4201.0 69 2590.0 70 1858.5 71 1482.0 72 1255.0 73 1051.0 74 863.5 75 737.5 76 504.5 77 271.0 78 154.5 79 87.5 80 47.0 81 31.0 82 17.0 83 9.0 84 6.0 85 4.5 86 1.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014302864324435857 3 0.0018757854851719157 4 0.001250523656781277 5 0.12348921110715112 6 0.0 7 0.0 8 1.5631545709765963E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 3.907886427441491E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.3447318564648946E-4 24-25 5.861829641162237E-4 26-27 0.005861829641162237 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010473135625543195 36-37 9.378927425859579E-4 38-39 0.007034195569394684 40-41 0.009652479475780483 42-43 0.005080252355673939 44-45 0.0031263091419531925 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.907886427441491E-5 68-69 6.643406926650535E-4 70-71 0.0 72-73 3.907886427441491E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.001719470028074256 84-85 0.0033998611918740975 86-87 0.002188416399367235 88-89 0.0 90-91 0.0024619684492881394 92-93 0.0 94-95 0.0 96-97 3.907886427441491E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1279464.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.965704724492745 #Duplication Level Percentage of deduplicated Percentage of total 1 79.22597912540085 43.5471177511561 2 12.07954841132439 13.27921782364144 3 3.632452537777288 5.989809408516022 4 1.6102338625475863 3.540305561046803 5 0.8116513785627346 2.2306495006653377 6 0.5122525449018077 1.6893793276465638 7 0.34630677837771795 1.3324497287079997 8 0.23952055096661864 1.0532332703903182 9 0.189797784833673 0.9389132098677431 >10 1.1721211357123995 12.344271990665625 >50 0.11189211930072061 4.205534398909809 >100 0.06412071743710956 6.2873308265860395 >500 0.0024169620197164957 0.8235919813065098 >1k 0.0014217423645391152 1.3391282087169893 >5k 2.84348472907823E-4 1.3990670121767195 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9491 0.7417950016569438 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8412 0.6574628125527565 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4933 0.3855520749313775 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2278 0.17804330563423434 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1741 0.1360726054035127 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1569 0.12262947609311399 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 1291 0.1009016275565393 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.815772854882982E-5 0.0 18-19 0.0 0.0 0.0 7.815772854882982E-5 0.0 20-21 0.0 0.0 0.0 1.9539432137207456E-4 0.0 22-23 0.0 0.0 0.0 3.5170977846973417E-4 0.0 24-25 0.0 0.0 0.0 8.988138783115429E-4 0.0 26-27 0.0 0.0 0.0 0.0016803911637998411 0.0 28-29 0.0 0.0 0.0 0.005275646677046013 0.0 30-31 0.0 0.0 0.0 0.014068391138789368 0.0 32-33 0.0 0.0 0.0 0.03329519236180151 0.0 34-35 0.0 0.0 0.0 0.0600251355255013 0.0 36-37 0.0 0.0 0.0 0.10504398716962728 0.0 38-39 0.0 0.0 0.0 0.17308028987138363 0.0 40-41 0.0 0.0 0.0 0.24756460517841847 0.0 42-43 0.0 0.0 0.0 0.3210719488785929 0.0 44-45 0.0 0.0 0.0 0.41060162693127744 0.0 46-47 0.0 0.0 0.0 0.5122066740447563 0.0 48-49 0.0 0.0 0.0 0.626434194318871 0.0 50-51 0.0 0.0 0.0 0.7439834180563111 0.0 52-53 0.0 0.0 0.0 0.854732919410003 0.0 54-55 0.0 0.0 0.0 0.9745487172753591 0.0 56-57 0.0 0.0 0.0 1.1160923636772897 0.0 58-59 0.0 0.0 0.0 1.268030987976215 0.0 60-61 0.0 0.0 0.0 1.4339207668211063 0.0 62-63 0.0 0.0 0.0 1.5922683248610356 0.0 64-65 0.0 0.0 0.0 1.747802204673207 0.0 66-67 0.0 0.0 0.0 1.9286982673994735 0.0 68-69 0.0 0.0 0.0 2.1346829609899145 0.0 70-71 0.0 0.0 0.0 2.352938417962522 0.0 72-73 0.0 0.0 0.0 2.5711547960708545 0.0 74-75 0.0 0.0 0.0 2.7872609155083694 0.0 76-77 0.0 0.0 0.0 3.018568713148631 0.0 78-79 0.0 0.0 0.0 3.2725422520680536 0.0 80-81 0.0 0.0 0.0 3.5525032357299615 0.0 82-83 0.0 0.0 0.0 3.835942238312293 0.0 84-85 0.0 0.0 0.0 4.1125815185108765 0.0 86-87 0.0 0.0 0.0 4.399146830235161 0.0 88-89 0.0 0.0 0.0 4.708612356424252 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4110 0.0 54.284725 1 GTATCAA 7105 0.0 40.440777 1 ATCAACG 9230 0.0 30.970726 3 TATCAAC 9285 0.0 30.945795 2 TCAACGC 9420 0.0 30.346052 4 CAACGCA 9530 0.0 30.044514 5 AACGCAG 9805 0.0 29.160776 6 TACACCG 70 0.00833855 27.178381 5 TGGTATC 1890 0.0 26.176897 2 GTGGTAT 1945 0.0 26.170424 1 ACGCAGA 11350 0.0 25.107622 7 GTACATG 8305 0.0 24.917028 1 CGCAGAG 11500 0.0 24.738832 8 TACATGG 8415 0.0 24.195591 2 GAGTACT 6675 0.0 24.014452 12-13 GCAGAGT 12080 0.0 23.433086 9 ACACCGT 165 2.4323526E-6 23.028 6 ACATGGG 8580 0.0 22.950474 3 GTACTTT 6960 0.0 22.89462 14-15 AGTACTT 6780 0.0 21.646067 12-13 >>END_MODULE