FastQCFastQC Report
Thu 26 May 2016
SRR938254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282260
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98050.7646655124545724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85510.6668694336562008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48880.38120194032411525No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21930.1710261569416499No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16930.13203250510816839No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15080.11760485392978022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42700.055.3140531
GTATCAA77600.043.59561
ATCAACG100550.033.3221173
TATCAAC100950.033.1900832
TCAACGC101800.032.8195884
CAACGCA103650.032.2796555
AACGCAG107050.031.2495486
ACGCAGA120000.027.7914947
CGCAGAG120750.027.3434738
GTGGTAT18550.026.494241
TGGTATC18350.025.640392
GCAGAGT126550.025.6397979
GTACATG86300.025.5993181
TACATGG86800.025.076752
GAGTACT70100.024.6005412-13
ACATGGG86250.024.2999293
GTACTTT73900.023.01637814-15
AGTACTT71950.022.5484112-13
CAGAGTA123450.022.08817910-11
CATGGGG37250.021.8170174
Produced by FastQC (version 0.11.5)