##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938254_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1282260 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.804855489526304 33.0 31.0 34.0 30.0 34.0 2 31.98776457192769 33.0 31.0 34.0 30.0 34.0 3 32.048649259900486 34.0 31.0 34.0 30.0 34.0 4 35.59105251665029 37.0 35.0 37.0 33.0 37.0 5 35.602243694726496 37.0 35.0 37.0 33.0 37.0 6 35.63467861432159 37.0 35.0 37.0 33.0 37.0 7 35.58283967370112 37.0 35.0 37.0 33.0 37.0 8 35.55824871710885 37.0 35.0 37.0 33.0 37.0 9 37.163557312869465 39.0 37.0 39.0 34.0 39.0 10-11 37.07704482710214 39.0 37.0 39.0 33.0 39.0 12-13 36.95982640026204 39.0 37.0 39.0 33.0 39.0 14-15 38.088770998081515 40.0 38.0 41.0 33.0 41.0 16-17 37.93114734921154 40.0 38.0 41.0 33.0 41.0 18-19 38.114789902203924 40.0 38.0 41.0 33.0 41.0 20-21 38.11864247500507 40.0 38.0 41.0 33.0 41.0 22-23 38.01893999656856 40.0 38.0 41.0 33.0 41.0 24-25 37.89472688846256 40.0 38.0 41.0 33.0 41.0 26-27 37.71386029354421 40.0 38.0 41.0 32.5 41.0 28-29 37.49184525759206 40.0 37.0 41.0 32.0 41.0 30-31 37.24456545474396 40.0 37.0 41.0 31.5 41.0 32-33 36.955444683605506 39.5 36.0 41.0 31.0 41.0 34-35 36.69943537192145 39.0 35.0 41.0 30.0 41.0 36-37 36.423858265874316 39.0 35.0 41.0 30.0 41.0 38-39 36.11764813688332 39.0 35.0 40.0 30.0 41.0 40-41 35.86399911094474 39.0 35.0 40.0 29.0 41.0 42-43 35.62875898803675 39.0 35.0 40.0 27.5 41.0 44-45 35.28599036076927 38.0 35.0 40.0 26.5 41.0 46-47 34.779534181835196 38.0 34.0 40.0 24.0 41.0 48-49 34.31348751423268 38.0 34.0 40.0 23.0 41.0 50-51 33.58216274390529 37.5 33.0 39.5 21.0 40.5 52-53 33.70005927035079 38.0 33.0 39.5 21.5 40.5 54-55 33.78204069377505 38.0 33.0 40.0 20.0 41.0 56-57 33.472961411882146 38.0 33.0 40.0 18.5 41.0 58-59 33.145461138926585 37.0 33.0 40.0 16.0 41.0 60-61 32.71612894420788 36.5 32.0 40.0 12.0 41.0 62-63 32.161887214761435 36.0 31.5 39.0 8.0 41.0 64-65 31.64045045466598 35.5 31.0 39.0 4.5 40.0 66-67 31.101985166814842 35.0 30.5 38.5 2.0 40.0 68-69 30.499048554895264 35.0 29.5 38.0 2.0 40.0 70-71 29.940791649119525 34.5 29.0 37.0 2.0 39.5 72-73 29.288523778328887 34.0 28.5 36.5 2.0 39.0 74-75 28.584386551869358 34.0 26.5 36.0 2.0 39.0 76-77 27.89734297256407 33.5 26.0 35.5 2.0 38.0 78-79 27.15930349539095 33.0 25.0 35.0 2.0 37.0 80-81 26.46453020448271 32.5 23.5 35.0 2.0 36.5 82-83 25.717285495921264 32.0 21.0 35.0 2.0 36.0 84-85 25.04186358460843 31.5 18.5 34.5 2.0 35.5 86-87 24.31513694570524 31.0 12.0 34.0 2.0 35.0 88-89 23.491576591330933 30.5 4.5 34.0 2.0 35.0 90-91 22.645661176360488 30.0 2.0 34.0 2.0 35.0 92-93 21.70176719230109 29.0 2.0 34.0 2.0 35.0 94-95 20.721324848314694 29.0 2.0 34.0 2.0 35.0 96-97 19.486765944504235 26.5 2.0 33.5 2.0 35.0 98-99 17.984218489229953 24.5 2.0 33.0 2.0 35.0 100-101 14.443580865035171 2.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33877986033560603 1101 2 0.3812462121748226 1101 3 0.2541670384138399 1101 4 0.2503514025703879 1101 5 0.35890809586101113 1101 6 0.2555208353969505 1101 7 0.23182939340673414 1101 8 0.27833428519031145 1101 9 0.5109168413910155 1101 10-11 0.5072541593716196 1101 12-13 0.5719883071236751 1101 14-15 0.6110052570424926 1101 16-17 0.8062809866360112 1101 18-19 0.5518983878854797 1101 20-21 0.6164902094745557 1101 22-23 0.5882582385035846 1101 24-25 0.47928894853720294 1101 26-27 0.6870953557986113 1101 28-29 0.7214209157685971 1101 30-31 0.8300255525823914 1101 32-33 0.7958975985397956 1101 34-35 0.8230062232582824 1101 36-37 0.8589308591554996 1101 38-39 0.8391768882512665 1101 40-41 0.9672740062447858 1101 42-43 1.1527439927525904 1101 44-45 1.1698576789109936 1101 46-47 1.3673028114939783 1101 48-49 1.2010006806267555 1101 50-51 1.2336859822536468 1101 52-53 1.156422860047364 1101 54-55 1.064956418069336 1101 56-57 1.0721774045546653 1101 58-59 1.1233644529972295 1101 60-61 1.3213639431292918 1101 62-63 1.2060631889375983 1101 64-65 1.133231588626785 1101 66-67 1.1331854860344386 1101 68-69 1.5699097098214168 1101 70-71 1.654770721605555 1101 72-73 1.7675755415215058 1101 74-75 2.053967924731893 1101 76-77 1.818915081590582 1101 78-79 2.125153159064471 1101 80-81 2.4927600419566787 1101 82-83 2.5657595627779664 1101 84-85 2.6881011827570447 1101 86-87 2.778506046173945 1101 88-89 3.322672435438779 1101 90-91 3.913011393979911 1101 92-93 4.1754498962832045 1101 94-95 4.2893031165450175 1101 96-97 4.439447106678987 1101 98-99 4.943521924793821 1101 100-101 4.394103409568871 1102 1 0.30155942174388883 1102 2 0.1696418516517042 1102 3 0.2939297853257443 1102 4 0.2364731852334998 1102 5 0.2599960500065919 1102 6 0.4326886720043035 1102 7 0.20852562601633196 1102 8 0.32238500075868615 1102 9 0.3612115938896068 1102 10-11 0.29559139250724087 1102 12-13 0.47190269285946584 1102 14-15 0.5367990935410205 1102 16-17 0.6102290376418011 1102 18-19 0.5104538285631364 1102 20-21 0.42940562911142166 1102 22-23 0.6285694971421023 1102 24-25 0.5332792625166363 1102 26-27 0.657149953030661 1102 28-29 0.674949783374835 1102 30-31 0.5616545816223137 1102 32-33 0.8044797029819435 1102 34-35 0.6556016777212861 1102 36-37 0.7597626960530661 1102 38-39 0.7193978165855981 1102 40-41 1.1217732897694859 1102 42-43 1.1021481392400503 1102 44-45 1.104196617076454 1102 46-47 1.0150940723091324 1102 48-49 1.333213047534663 1102 50-51 1.5002699368345631 1102 52-53 1.3791871850188002 1102 54-55 1.3132744242727412 1102 56-57 1.4274225479404237 1102 58-59 1.4193057564381206 1102 60-61 1.3394176697077995 1102 62-63 1.2391995369087994 1102 64-65 1.4705198656397158 1102 66-67 1.3919676594033241 1102 68-69 1.488251840383171 1102 70-71 1.6652345259987236 1102 72-73 1.9463733140995494 1102 74-75 1.9503100915708735 1102 76-77 2.26569565876132 1102 78-79 2.4659327567499822 1102 80-81 2.565738583974781 1102 82-83 2.698925658078249 1102 84-85 2.804553995149348 1102 86-87 3.221244977083959 1102 88-89 3.4431211847812513 1102 90-91 3.5236442502676617 1102 92-93 3.782312097222782 1102 94-95 4.080972050538595 1102 96-97 4.178542715320223 1102 98-99 4.229531973586695 1102 100-101 3.9497029814975484 1103 1 0.4114990315509388 1103 2 0.39872198359066147 1103 3 0.4882897965735822 1103 4 0.29359079212146355 1103 5 0.39490675911154227 1103 6 0.4565323091632365 1103 7 0.465457554262251 1103 8 0.472956096485845 1103 9 0.5397460716461069 1103 10-11 0.5362103808264465 1103 12-13 0.6352900783167215 1103 14-15 0.8337054909736494 1103 16-17 0.8391783911141246 1103 18-19 0.6905460430041117 1103 20-21 0.69604685423338 1103 22-23 0.7540820103650034 1103 24-25 0.7618755587633359 1103 26-27 0.7024301001452784 1103 28-29 0.8123421589456044 1103 30-31 0.7850974028664481 1103 32-33 0.9643258039536349 1103 34-35 1.203416159762675 1103 36-37 1.151425010876558 1103 38-39 1.3327961374436441 1103 40-41 1.4201981457123125 1103 42-43 1.612552391995095 1103 44-45 1.5947301307323158 1103 46-47 1.7154770345083463 1103 48-49 1.8207511540588612 1103 50-51 1.9219871964677537 1103 52-53 1.7865598768681323 1103 54-55 1.9497442090908308 1103 56-57 2.0206390287052756 1103 58-59 2.20588422574982 1103 60-61 2.1024868126848233 1103 62-63 2.3307574074961366 1103 64-65 2.3419293718505756 1103 66-67 2.3082261454975104 1103 68-69 2.5219359992276793 1103 70-71 2.9072782408213236 1103 72-73 3.0662944899452533 1103 74-75 3.242480790945546 1103 76-77 3.15985526205176 1103 78-79 3.268913880909185 1103 80-81 3.4911069284443705 1103 82-83 3.4326928367002125 1103 84-85 3.4717727879703695 1103 86-87 3.336730397293472 1103 88-89 3.3048736162341434 1103 90-91 3.863228615768925 1103 92-93 4.350769045219376 1103 94-95 4.737515214127729 1103 96-97 5.087097769483936 1103 98-99 5.144439826097152 1103 100-101 4.494116777063578 1104 1 0.27722851170099716 1104 2 0.2029516904793951 1104 3 0.23489077773366063 1104 4 0.08992587065549884 1104 5 0.2716103910528389 1104 6 0.27729332660150163 1104 7 0.3301976292921367 1104 8 0.37014865945735664 1104 9 0.41934371356390443 1104 10-11 0.3966795237542584 1104 12-13 0.3997601281992331 1104 14-15 0.5002429853051851 1104 16-17 0.6860246622935762 1104 18-19 0.5189749501237131 1104 20-21 0.5624627341855657 1104 22-23 0.29255544071501305 1104 24-25 0.4612138940892194 1104 26-27 0.45676699258869036 1104 28-29 0.5523306158858645 1104 30-31 0.5390488395651332 1104 32-33 0.5613122408584417 1104 34-35 0.49868556653186147 1104 36-37 0.44901127463547397 1104 38-39 0.34590163276457986 1104 40-41 0.5668300457824031 1104 42-43 0.5791805817959812 1104 44-45 0.671802728333013 1104 46-47 0.5849724378970578 1104 48-49 0.6604159930744018 1104 50-51 0.7337714237011284 1104 52-53 0.576799807609774 1104 54-55 0.7610914490628033 1104 56-57 0.9063338796761258 1104 58-59 0.7096954967231426 1104 60-61 0.8328533048314171 1104 62-63 0.9692328991068067 1104 64-65 1.2290114512503365 1104 66-67 1.0353935610888847 1104 68-69 1.0386280881092347 1104 70-71 1.3933399961909139 1104 72-73 1.5601622394608619 1104 74-75 1.4709406169751489 1104 76-77 1.6100593268577974 1104 78-79 1.7248482503695435 1104 80-81 1.7396640459774524 1104 82-83 1.989435220342049 1104 84-85 2.2408889193963475 1104 86-87 2.377083697371802 1104 88-89 2.5282998437148727 1104 90-91 2.6134553544423014 1104 92-93 2.8267532971878815 1104 94-95 3.162087502539322 1104 96-97 3.5606600401686848 1104 98-99 3.728155866827663 1104 100-101 3.6050332135561334 1105 1 0.42505564399647966 1105 2 0.28873037654058464 1105 3 0.25621673794283595 1105 4 0.2639716721822154 1105 5 0.25326573756964166 1105 6 0.319867107932275 1105 7 0.36055767016489426 1105 8 0.2861010547209233 1105 9 0.3901437376067065 1105 10-11 0.46845929174127576 1105 12-13 0.3546438211727221 1105 14-15 0.5073476315755485 1105 16-17 0.6583063236507911 1105 18-19 0.4312698350188171 1105 20-21 0.6015202565754194 1105 22-23 0.4514302378539199 1105 24-25 0.557099570593401 1105 26-27 0.4832801860730953 1105 28-29 0.6233049502009536 1105 30-31 0.5070809366980313 1105 32-33 0.7361574653580547 1105 34-35 0.6080622570409631 1105 36-37 0.6993027935391254 1105 38-39 0.7499077636776121 1105 40-41 0.8145129213008246 1105 42-43 0.9829372686986702 1105 44-45 1.000299452502027 1105 46-47 0.9831481907371185 1105 48-49 0.968752832648839 1105 50-51 1.1886851701698475 1105 52-53 1.2009477910202477 1105 54-55 1.2138503599241304 1105 56-57 1.4403664576649504 1105 58-59 1.2354693742250973 1105 60-61 1.296975442235997 1105 62-63 1.2731218213884716 1105 64-65 1.4067868915340398 1105 66-67 1.664692713580326 1105 68-69 1.8248455552006533 1105 70-71 1.9486654755441606 1105 72-73 2.036269710759772 1105 74-75 2.0870733332100464 1105 76-77 2.2084424488148784 1105 78-79 2.3002855287250163 1105 80-81 2.5458069804007266 1105 82-83 2.511936272214381 1105 84-85 2.6484503787940774 1105 86-87 3.073573449129931 1105 88-89 3.445129801038906 1105 90-91 3.5566121735803655 1105 92-93 3.646952251326244 1105 94-95 3.975091649921662 1105 96-97 4.164071111876325 1105 98-99 4.30435349861224 1105 100-101 4.3479315726352965 1106 1 0.07225407707191778 1106 2 0.27764760179747583 1106 3 0.09208196404981805 1106 4 0.23943678111448463 1106 5 0.2256010180709609 1106 6 0.18480889392759536 1106 7 0.2745830235557776 1106 8 0.2921005147982356 1106 9 0.2848277966765451 1106 10-11 0.23531493134862558 1106 12-13 0.38600844704650683 1106 14-15 0.622950248477423 1106 16-17 0.4053088911554852 1106 18-19 0.4212091798743387 1106 20-21 0.3660510695036905 1106 22-23 0.2703852576989476 1106 24-25 0.3145341336868128 1106 26-27 0.36256467363477185 1106 28-29 0.32888453423072406 1106 30-31 0.2811425338352933 1106 32-33 0.3176099722650392 1106 34-35 0.35509497980215343 1106 36-37 0.46462511015209174 1106 38-39 0.3806106970660821 1106 40-41 0.5624359421108167 1106 42-43 0.37612103195979785 1106 44-45 0.43590189708815075 1106 46-47 0.6707983208355088 1106 48-49 0.8205994831178316 1106 50-51 0.8396834527561907 1106 52-53 0.775086068666873 1106 54-55 0.4345345683342927 1106 56-57 0.7417811699837955 1106 58-59 0.865866734347783 1106 60-61 1.1102046968554973 1106 62-63 0.8513543212879569 1106 64-65 1.0886759316778694 1106 66-67 1.0154543492014376 1106 68-69 0.8497595409214256 1106 70-71 1.074559076311541 1106 72-73 1.56878425708112 1106 74-75 1.6720861707335253 1106 76-77 1.836470754548973 1106 78-79 2.075827318166656 1106 80-81 2.2403184365262625 1106 82-83 2.4982690483934498 1106 84-85 2.4438314550782607 1106 86-87 2.108737062935866 1106 88-89 2.5032205999521686 1106 90-91 2.8987373437421695 1106 92-93 3.0867359376196752 1106 94-95 3.4474447363839644 1106 96-97 3.4184881389701083 1106 98-99 3.6228194289162587 1106 100-101 3.805458611848618 1107 1 0.24549017130124184 1107 2 0.11129654722716253 1107 3 -0.0819235029195724 1107 4 0.08586200998786353 1107 5 0.14803634216308836 1107 6 0.09985026750639037 1107 7 0.12445268340457005 1107 8 0.12256057676304977 1107 9 0.07429268674196976 1107 10-11 0.03933920399379787 1107 12-13 0.00587306147009059 1107 14-15 0.10280404035472657 1107 16-17 0.17355023284544302 1107 18-19 0.1468682794952727 1107 20-21 0.21097249956503816 1107 22-23 0.15143799331320196 1107 24-25 0.008454560123531962 1107 26-27 0.045066822308818644 1107 28-29 0.2568561750095526 1107 30-31 0.3729236781420937 1107 32-33 0.22657171767638573 1107 34-35 0.18646884253382012 1107 36-37 0.25872179884607505 1107 38-39 0.20462419084830685 1107 40-41 0.16730488664499887 1107 42-43 0.2560945161753807 1107 44-45 0.39451166402664484 1107 46-47 0.38983284268318386 1107 48-49 0.1598988679472626 1107 50-51 0.14235627222060998 1107 52-53 0.049413318003708184 1107 54-55 0.3669392485668155 1107 56-57 0.454209952496484 1107 58-59 0.4579989834607474 1107 60-61 0.6653641901724896 1107 62-63 0.6471393708849149 1107 64-65 0.9206963077695463 1107 66-67 0.8835656237610259 1107 68-69 0.9412051426102224 1107 70-71 1.2920600400751177 1107 72-73 1.269880763397186 1107 74-75 1.1515367108233612 1107 76-77 1.3797117959159984 1107 78-79 1.7307057638262258 1107 80-81 1.821469241227902 1107 82-83 2.1615950320056463 1107 84-85 2.271327875774986 1107 86-87 2.2536325014128025 1107 88-89 2.5728046035516776 1107 90-91 2.573285303129772 1107 92-93 3.1923510481221307 1107 94-95 3.5480422698044443 1107 96-97 3.6097275007128964 1107 98-99 3.694637016045405 1107 100-101 3.942791918971949 1108 1 0.26027015114672025 1108 2 0.09710176669803161 1108 3 0.17712965726236973 1108 4 0.11554450238766378 1108 5 0.11099766196711869 1108 6 0.275260065475301 1108 7 0.15931429538573383 1108 8 0.15437999735235053 1108 9 0.12454377316344534 1108 10-11 0.08014523682833641 1108 12-13 0.20051257235331832 1108 14-15 0.3174179672534052 1108 16-17 0.46578546184429115 1108 18-19 0.2452314115158174 1108 20-21 0.2444350204008785 1108 22-23 0.23318685196327493 1108 24-25 0.09781259436085321 1108 26-27 0.1296228419394012 1108 28-29 0.25512434418075003 1108 30-31 0.18993480960490672 1108 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0.9773119022448142 2201 36-37 1.0476760453474938 2201 38-39 1.1217482023119416 2201 40-41 1.189439305005834 2201 42-43 1.2125519631988624 2201 44-45 1.471225236023237 2201 46-47 1.6357217914023252 2201 48-49 1.6478004784903888 2201 50-51 1.6594283977833015 2201 52-53 1.6605516697080915 2201 54-55 1.603554706880388 2201 56-57 1.5593528972620732 2201 58-59 1.7853624664846066 2201 60-61 1.7005027724808812 2201 62-63 1.7253215575576704 2201 64-65 1.7768020836866398 2201 66-67 1.7128871606297373 2201 68-69 1.9757636488668453 2201 70-71 2.0120164392768025 2201 72-73 2.2005934724117786 2201 74-75 2.370990595371584 2201 76-77 2.444882983728693 2201 78-79 2.2325300911222534 2201 80-81 2.6791743866688975 2201 82-83 2.7224441777604547 2201 84-85 2.583039409184636 2201 86-87 2.8987733541008254 2201 88-89 3.152658669254798 2201 90-91 3.303049207421978 2201 92-93 3.457976252426583 2201 94-95 3.6018042056418516 2201 96-97 4.112616291337535 2201 98-99 4.306941166085402 2201 100-101 3.7609986994428795 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0.9833009448510879 2203 20-21 1.0123282997703527 2203 22-23 1.0177671312714338 2203 24-25 1.0113090457207647 2203 26-27 0.9917592233270369 2203 28-29 1.0371613507358077 2203 30-31 1.2018838344147227 2203 32-33 1.1089456713802903 2203 34-35 1.2873686330332461 2203 36-37 1.1258257027654466 2203 38-39 1.3379394489909942 2203 40-41 1.3240650615828073 2203 42-43 1.3538196166616387 2203 44-45 1.4178753229172187 2203 46-47 1.4249788977325935 2203 48-49 1.6035694403569067 2203 50-51 1.6244360884007492 2203 52-53 1.4486052443470356 2203 54-55 1.251279482653949 2203 56-57 1.3655793010494577 2203 58-59 1.53383433081013 2203 60-61 1.6546936595985997 2203 62-63 1.5853423322125906 2203 64-65 1.814765767991787 2203 66-67 1.9655339315691869 2203 68-69 1.9101437051981165 2203 70-71 2.1308314849211136 2203 72-73 2.281285028713093 2203 74-75 2.2982892909629626 2203 76-77 2.1385367261629256 2203 78-79 2.157586522276244 2203 80-81 2.3831954809338924 2203 82-83 2.472713848761831 2203 84-85 2.486087690281696 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68-69 0.879807387261728 2205 70-71 1.0169860862311815 2205 72-73 0.9503918481643545 2205 74-75 1.1050122645077565 2205 76-77 1.028289777059154 2205 78-79 1.214407666129599 2205 80-81 1.4912268277289726 2205 82-83 1.4623179516036942 2205 84-85 1.4324198444429328 2205 86-87 1.3468558918780218 2205 88-89 1.4172672056190585 2205 90-91 1.4795129369391447 2205 92-93 1.4568759154483821 2205 94-95 1.345320657555252 2205 96-97 1.7980405775251818 2205 98-99 1.7860328879252165 2205 100-101 1.2819010382841505 2206 1 0.06318247899354645 2206 2 0.00720365898333597 2206 3 0.1802390515071295 2206 4 0.032233562963185136 2206 5 0.12466914332419066 2206 6 0.12043081903033226 2206 7 0.2141043524902031 2206 8 0.1541280658777069 2206 9 0.13304450096548237 2206 10-11 0.038236156098292895 2206 12-13 0.3102779961580424 2206 14-15 0.2559970737364381 2206 16-17 0.3192106102039389 2206 18-19 0.30797858428481817 2206 20-21 0.3177504386071277 2206 22-23 0.47378197009114587 2206 24-25 0.3320467226779513 2206 26-27 0.2681510022797937 2206 28-29 0.2963554493201528 2206 30-31 0.44575386682134166 2206 32-33 0.44595488168218367 2206 34-35 0.5050029499358573 2206 36-37 0.49452005719150094 2206 38-39 0.6411408817046151 2206 40-41 0.5561365267030496 2206 42-43 0.29490438555127696 2206 44-45 0.6450704448684448 2206 46-47 0.915036035716561 2206 48-49 0.8272936429835482 2206 50-51 0.7991196974746018 2206 52-53 0.8328385719949907 2206 54-55 0.8322150105406934 2206 56-57 1.0551315780407577 2206 58-59 0.622251523373663 2206 60-61 0.8109302279083366 2206 62-63 0.8046720269081362 2206 64-65 0.7886571205416786 2206 66-67 0.9078254205679919 2206 68-69 0.5526347507379441 2206 70-71 0.7027494450098075 2206 72-73 1.0626819245002324 2206 74-75 1.1677486121296 2206 76-77 1.0355681094138198 2206 78-79 1.187919116511278 2206 80-81 1.414306686801197 2206 82-83 1.4907677460840212 2206 84-85 1.5381420992873274 2206 86-87 1.664815375142851 2206 88-89 1.487173253769381 2206 90-91 1.63876425813703 2206 92-93 1.789230583680915 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68-69 -1.615832974566242 2316 70-71 -1.8716607426577525 2316 72-73 -1.9827068154461465 2316 74-75 -2.0350963043480235 2316 76-77 -1.934716485347984 2316 78-79 -2.8040782025235735 2316 80-81 -2.719007398577233 2316 82-83 -2.502978387139322 2316 84-85 -2.569248084255676 2316 86-87 -2.3185069593591123 2316 88-89 -2.7364037724215855 2316 90-91 -2.4580783898316234 2316 92-93 -3.0012416786412146 2316 94-95 -3.2044864562118143 2316 96-97 -3.4040683801728733 2316 98-99 -3.338905204291798 2316 100-101 -3.0136380198989308 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3704.0 3 1180.0 4 1251.0 5 1408.0 6 1617.0 7 2075.0 8 2499.0 9 3303.0 10 3991.0 11 4945.0 12 6314.0 13 7878.0 14 9235.0 15 11369.0 16 14407.0 17 18907.0 18 17929.0 19 13381.0 20 13397.0 21 14369.0 22 15389.0 23 17150.0 24 19460.0 25 21725.0 26 24597.0 27 28438.0 28 32532.0 29 37812.0 30 44466.0 31 52945.0 32 64339.0 33 81353.0 34 105065.0 35 139799.0 36 169989.0 37 181283.0 38 88425.0 39 4334.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.03581938007088 18.558622505100267 14.689079633094224 21.716478481734626 2 16.067505320908516 19.997019667076525 39.896983466174 24.038491545840955 3 19.387423884444804 25.306490928555153 29.343619401936948 25.962465785063095 4 12.895105637209767 16.926689118540274 36.303368717402904 33.874836526847055 5 13.879574907822809 37.586440508637025 32.85558854473989 15.678396038800276 6 31.55849913055966 35.56120266857309 17.373119464537137 15.507178736330118 7 28.922454418915294 33.688650450349236 19.55835814467352 17.830536986061958 8 27.468390773210917 33.76019897858078 18.958904494338192 19.81250575387011 9 27.7974350912043 16.53435365843192 19.813713687393843 35.85449756296994 10-11 25.844702471307485 26.635285232038868 25.641019639144712 21.878992657508935 12-13 26.819378564860813 24.26752240881115 26.81301979527883 22.100079231049207 14-15 23.67164061497365 25.23458616289788 25.93498018573412 25.158793036394357 16-17 22.295146043116638 28.747506395408255 26.337457549234305 22.619890012240802 18-19 22.024954785835444 26.284409968806514 29.848136583447637 21.8424986619104 20-21 23.235105818939537 25.298894077283897 30.007151220018425 21.458848883758147 22-23 22.849708389412292 25.25671484580724 30.043770846743517 21.849805918036942 24-25 22.384078777386694 25.7448685489368 30.131148679519733 21.739903994156776 26-27 22.038251400231278 26.08514997963442 30.059286471167486 21.817312148966817 28-29 21.700195088878374 26.076528986165894 30.356352208569167 21.86692371638657 30-31 21.97227669625104 25.728221430652926 30.272298191115482 22.027203681980556 32-33 21.837771582875543 26.00466256117856 30.04898185923528 22.108583996710614 34-35 22.435072927504752 26.06992005224517 29.77370178040096 21.721305239849116 36-37 22.460002777530715 25.807824522475375 29.678578840521364 22.05359385947255 38-39 22.034930910841112 26.00452988676063 29.627893243822502 22.33264595857576 40-41 22.2087705243484 25.863896726323038 29.88600206091799 22.041330688410575 42-43 22.354108511651543 25.824863518947044 29.465554354698238 22.355473614703175 44-45 22.141979665934784 26.14549737341908 29.42113822406471 22.291384736581428 46-47 22.317426155087674 26.40467296681986 28.8954943984698 22.382406479622663 48-49 22.528635567428292 26.440164174522995 28.64079392883456 22.390406329214148 50-51 22.505266868995555 26.662836925568083 28.31570950438782 22.51618670104854 52-53 22.669074759021765 26.652996023605645 28.287974254055566 22.38995496331702 54-55 22.35406438066014 26.514170897857447 28.47650221735327 22.65526250412914 56-57 22.422592161288787 26.892813435740397 28.282008902769185 22.402585500201628 58-59 22.56742690649171 26.38854168657257 28.324351138994764 22.719680267940955 60-61 22.536697115320862 26.50616318097046 28.59383689702968 22.363302806678995 62-63 22.293232100382237 26.546427837397562 28.618484555514 22.541855506706202 64-65 22.142597515241143 26.37926892429036 28.643717591119266 22.834415969349234 66-67 22.249788830765898 26.679078140026064 28.24290038505725 22.828232644150788 68-69 22.219137010939846 26.507017382392352 28.43042129253367 22.843424314134133 70-71 22.257582919535174 26.546458496161545 28.377037595181378 22.818920989121906 72-73 22.366184703056906 26.60144284881911 28.090272821511224 22.94209962661275 74-75 22.24196443126448 26.796512856831978 27.982709360797013 22.978813351106524 76-77 22.378897574539195 26.65511035368947 28.004820113444257 22.96117195832708 78-79 22.52551594446726 26.656776569204748 27.86589558225414 22.951811904073853 80-81 22.400257400257402 27.158652158652156 27.76939276939277 22.67169767169767 82-83 22.68593934208284 26.972060886449995 27.563204342279786 22.778795429187387 84-85 22.681786557762685 26.701065744851448 27.47802837202482 23.139119325361047 86-87 22.6925760081484 26.47770712310616 27.668771488701772 23.160945380043664 88-89 22.693558540883487 26.616956562100448 27.546202937984976 23.143281959031096 90-91 22.754589116088322 26.787854722554528 27.47575894332045 22.981797218036697 92-93 22.918476126441117 26.29572270034644 27.53215492082707 23.25364625238537 94-95 22.80200834417702 26.240548830910278 27.543390344595164 23.41405248031754 96-97 22.992455460381514 26.15271383833865 27.36643965050875 23.48839105077109 98-99 22.94322114019514 26.098945673642465 27.139191984748308 23.81864120141409 100-101 23.832166401179673 25.097209633448315 27.165352639490013 23.905271325881994 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1476.0 1 975.5 2 789.0 3 1038.5 4 766.5 5 684.5 6 1279.5 7 2541.0 8 4041.5 9 4740.0 10 3789.0 11 2592.0 12 2346.5 13 2444.5 14 2557.0 15 2572.5 16 2572.0 17 2653.5 18 2736.5 19 2813.5 20 3022.0 21 3334.5 22 3718.5 23 4326.5 24 4943.5 25 5639.5 26 6782.5 27 8148.5 28 9382.0 29 10848.0 30 12795.0 31 14922.5 32 17337.5 33 19536.5 34 21533.5 35 23462.5 36 25932.0 37 28480.5 38 29950.0 39 30764.5 40 31732.5 41 34329.0 42 37208.5 43 39385.5 44 41271.5 45 43220.0 46 44202.0 47 44872.0 48 47064.0 49 47857.5 50 47351.5 51 45951.0 52 44611.5 53 44923.5 54 45026.0 55 42959.5 56 39434.0 57 36693.0 58 33668.0 59 29537.0 60 25652.5 61 21885.5 62 18058.0 63 14461.5 64 11557.5 65 9577.5 66 7496.0 67 5915.5 68 4521.5 69 2980.5 70 2166.5 71 1676.5 72 1355.0 73 1129.0 74 910.0 75 757.0 76 522.0 77 309.5 78 194.5 79 122.5 80 72.0 81 38.0 82 23.0 83 12.5 84 9.5 85 8.5 86 3.0 87 3.0 88 2.5 89 2.0 90 2.5 91 1.5 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45669365027373543 2 0.04086534712148862 3 0.05115967120552774 4 0.054903061781541966 5 0.08048289738430583 6 0.07611560837895591 7 0.04585653455617426 8 0.04008547408481899 9 0.04499867421583766 10-11 0.04761124888868092 12-13 0.044101820223667584 14-15 0.037550886715642696 16-17 0.03751189306380921 18-19 0.045388610734172476 20-21 0.05151061407202907 22-23 0.045622572645173366 24-25 0.06047915399372982 26-27 0.053499290315536636 28-29 0.04184018841732566 30-31 0.04355590909799885 32-33 0.043750877357166255 34-35 0.04761124888868092 36-37 0.04258106780216181 38-39 0.025696816558264314 40-41 0.02394210222575765 42-43 0.023825121270257203 44-45 0.013647778141718527 46-47 0.02600876577293217 48-49 0.02585279116559824 50-51 0.014739600393056012 52-53 0.015129536911390826 54-55 0.016650289332896605 56-57 0.014583625785722084 58-59 0.014544632133888603 60-61 0.016455321073729195 62-63 0.015636454385226085 64-65 0.015909409948060456 66-67 0.00881256531436682 68-69 0.012321993979380158 70-71 0.018249029058069344 72-73 0.016611295681063124 74-75 0.013959727356386379 76-77 0.010333317735872599 78-79 0.013218847971550231 80-81 0.016377333770062234 82-83 0.01314086066788327 84-85 0.011854070157378379 86-87 0.0035484223168468177 88-89 0.028153416623773648 90-91 0.030843978600283876 92-93 0.017508149673233196 94-95 0.03181881989612091 96-97 0.026476689594933944 98-99 0.023708140314756758 100-101 0.14883876904839893 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1282260.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.76379914720926 #Duplication Level Percentage of deduplicated Percentage of total 1 80.39901408144613 45.63753486952857 2 11.452082562603373 13.001274288017505 3 3.424439526358451 5.831525923979266 4 1.4431117008412542 3.276660109341619 5 0.8065296505727028 2.289084354568889 6 0.4833398349049204 1.646172318503291 7 0.3243803522269187 1.2889142812776861 8 0.24149307515872448 1.0966451531001398 9 0.1720574819366605 0.8789972711784465 >10 1.0916099897246367 11.89440951631577 >50 0.10128480473090276 3.9123374852321353 >100 0.056649999635553665 5.6906302291169775 >500 0.0026252364598893245 0.9691685834400011 >1k 0.0011053627199533998 1.146971058042128 >5k 2.7634067998834994E-4 1.4396745583575836 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9805 0.7646655124545724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8551 0.6668694336562008 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4888 0.38120194032411525 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2193 0.1710261569416499 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1693 0.13203250510816839 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1508 0.11760485392978022 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.798730366696302E-5 0.0 12-13 0.0 0.0 0.0 1.5597460733392604E-4 0.0 14-15 0.0 0.0 0.0 1.5597460733392604E-4 0.0 16-17 0.0 0.0 0.0 2.3396191100088904E-4 0.0 18-19 0.0 0.0 0.0 2.7295556283437055E-4 0.0 20-21 0.0 0.0 0.0 3.509428665013336E-4 0.0 22-23 0.0 0.0 0.0 5.459111256687411E-4 0.0 24-25 0.0 0.0 0.0 0.00128679051050489 0.0 26-27 0.0 0.0 0.0 0.002144650850841483 0.0 28-29 0.0 0.0 0.0 0.005459111256687412 0.0 30-31 0.0 0.0 0.0 0.015090543259557346 0.0 32-33 0.0 0.0 0.0 0.03376850248779499 0.0 34-35 0.0 0.0 0.0 0.06071311590473071 0.0 36-37 0.0 0.0 0.0 0.10602373933523623 0.0 38-39 0.0 0.0 0.0 0.1735607443108262 0.0 40-41 0.0 0.0 0.0 0.24608893672110183 0.0 42-43 0.0 0.0 0.0 0.31768128148737385 0.0 44-45 0.0 0.0 0.0 0.4056899536755416 0.0 46-47 0.0 0.0 0.0 0.5093350802489355 0.0 48-49 0.0 0.0 0.0 0.6194921466785208 0.0 50-51 0.0 0.0 0.0 0.7379548609486375 0.0 52-53 0.0 0.0 0.0 0.8548968227972487 0.0 54-55 0.0 0.0 0.0 0.9740614228003681 0.0 56-57 0.0 0.0 0.0 1.111864988379892 0.0 58-59 0.0 0.0 0.0 1.2616785987241277 0.0 60-61 0.0 0.0 0.0 1.4223714379299048 0.0 62-63 0.0 0.0 0.0 1.5812315754995088 0.0 64-65 0.0 0.0 0.0 1.7382590114329388 0.0 66-67 0.0 0.0 0.0 1.9143933367647747 3.899365183348151E-5 68-69 0.0 0.0 0.0 2.1162634723067084 7.798730366696302E-5 70-71 0.0 0.0 0.0 2.333419119367367 7.798730366696302E-5 72-73 0.0 0.0 0.0 2.5478062171478486 7.798730366696302E-5 74-75 0.0 0.0 0.0 2.7609455180696583 7.798730366696302E-5 76-77 0.0 0.0 0.0 2.9927237845678727 7.798730366696302E-5 78-79 0.0 0.0 0.0 3.2463774897446696 7.798730366696302E-5 80-81 0.0 0.0 0.0 3.5235053733252224 7.798730366696302E-5 82-83 0.0 3.899365183348151E-5 0.0 3.8027779077566173 7.798730366696302E-5 84-85 0.0 7.798730366696302E-5 0.0 4.075967432501988 7.798730366696302E-5 86-87 0.0 7.798730366696302E-5 0.0 4.354226131985713 7.798730366696302E-5 88-89 0.0 7.798730366696302E-5 0.0 4.646483552477656 7.798730366696302E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4270 0.0 55.314053 1 GTATCAA 7760 0.0 43.5956 1 ATCAACG 10055 0.0 33.322117 3 TATCAAC 10095 0.0 33.190083 2 TCAACGC 10180 0.0 32.819588 4 CAACGCA 10365 0.0 32.279655 5 AACGCAG 10705 0.0 31.249548 6 ACGCAGA 12000 0.0 27.791494 7 CGCAGAG 12075 0.0 27.343473 8 GTGGTAT 1855 0.0 26.49424 1 TGGTATC 1835 0.0 25.64039 2 GCAGAGT 12655 0.0 25.639797 9 GTACATG 8630 0.0 25.599318 1 TACATGG 8680 0.0 25.07675 2 GAGTACT 7010 0.0 24.60054 12-13 ACATGGG 8625 0.0 24.299929 3 GTACTTT 7390 0.0 23.016378 14-15 AGTACTT 7195 0.0 22.54841 12-13 CAGAGTA 12345 0.0 22.088179 10-11 CATGGGG 3725 0.0 21.817017 4 >>END_MODULE