FastQCFastQC Report
Thu 26 May 2016
SRR938254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282260
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97340.759128413894218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85170.6642178653315239No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49960.38962456912014726No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22770.1775770904496748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17960.14006519738586556No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.1271972922808167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43750.054.5076641
GTATCAA76900.042.376951
TATCAAC99050.032.900432
ATCAACG99850.032.631743
TCAACGC101300.032.2584274
CAACGCA102850.031.8184575
TAGACCG600.00395243231.6645225
AACGCAG105850.031.0512756
ACGCAGA118200.027.6059977
CGCAGAG119550.027.1353448
GAGTACT67500.025.64826212-13
GCAGAGT125150.025.5036669
GTACATG86200.024.5236261
GTGGTAT19250.024.1839541
TGGTATC19450.024.179512
TACATGG86800.024.0804652
GTACTTT71250.023.79838814-15
ACATGGG86800.022.7634353
CAGAGTA123150.022.25387210-11
AGTACTT68900.021.92159312-13
Produced by FastQC (version 0.11.5)