##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938254_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1282260 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50091635081809 33.0 31.0 34.0 31.0 34.0 2 32.73068644424688 34.0 31.0 34.0 31.0 34.0 3 32.67523747133966 34.0 31.0 34.0 31.0 34.0 4 36.20323647310218 37.0 35.0 37.0 35.0 37.0 5 36.17356776316816 37.0 35.0 37.0 35.0 37.0 6 36.24339603512548 37.0 37.0 37.0 35.0 37.0 7 36.205228268837836 37.0 37.0 37.0 35.0 37.0 8 36.20548406719386 37.0 37.0 37.0 35.0 37.0 9 37.90064417512829 39.0 38.0 39.0 35.0 39.0 10-11 37.87590972189727 39.0 38.0 39.0 35.0 39.0 12-13 37.80805647840532 39.0 38.0 39.0 35.0 39.0 14-15 39.06173669926536 40.0 38.0 41.0 36.0 41.0 16-17 39.001588211439184 40.0 38.0 41.0 36.0 41.0 18-19 39.02258785269758 40.0 38.5 41.0 36.0 41.0 20-21 39.05059036388876 40.0 38.5 41.0 35.5 41.0 22-23 39.06122861198197 40.0 39.0 41.0 35.0 41.0 24-25 39.00062818773104 40.0 38.5 41.0 35.0 41.0 26-27 38.83142771356823 40.0 38.0 41.0 35.0 41.0 28-29 38.74466956779436 40.0 38.0 41.0 35.0 41.0 30-31 38.51165208304088 40.0 38.0 41.0 34.5 41.0 32-33 38.281922932946514 40.0 38.0 41.0 34.0 41.0 34-35 38.057698516681484 40.0 37.0 41.0 34.0 41.0 36-37 37.96199756679613 40.0 37.0 41.0 33.0 41.0 38-39 37.90181788404848 40.0 37.0 41.0 33.0 41.0 40-41 37.75783265484379 40.0 37.0 41.0 33.0 41.0 42-43 37.534331180883754 40.0 37.0 41.0 33.0 41.0 44-45 37.22622752015972 40.0 36.0 41.0 32.5 41.0 46-47 36.77243694726498 40.0 36.0 41.0 31.0 41.0 48-49 36.32924913824029 39.5 35.0 41.0 30.0 41.0 50-51 35.99848080732457 39.0 35.0 41.0 29.5 41.0 52-53 35.530829940885624 39.0 35.0 40.0 28.0 41.0 54-55 35.20883323194984 38.0 35.0 40.0 27.0 41.0 56-57 34.93814242041395 38.0 34.5 40.0 26.5 41.0 58-59 34.572289161324534 38.0 34.0 40.0 26.0 41.0 60-61 34.168097343752436 37.0 34.0 40.0 25.5 41.0 62-63 33.745493503657606 37.0 33.0 40.0 23.5 41.0 64-65 33.32073682404504 36.0 33.0 39.0 22.5 41.0 66-67 32.82510021368521 35.5 32.0 39.0 21.5 40.0 68-69 32.30664802770109 35.0 31.5 38.5 20.0 40.0 70-71 31.73116255673576 35.0 31.0 37.5 18.0 40.0 72-73 31.283711181819598 35.0 31.0 37.0 17.0 39.0 74-75 30.743302840297602 34.5 30.0 36.5 12.0 39.0 76-77 29.53380983575874 33.5 28.5 35.0 11.0 37.0 78-79 30.2348533058818 34.0 30.0 35.0 9.0 37.5 80-81 30.056615662970067 34.0 30.0 35.0 7.0 37.0 82-83 29.621170043516916 34.0 29.5 35.0 2.0 37.0 84-85 29.135763417715594 34.0 29.0 35.0 2.0 36.0 86-87 28.599925912061515 34.0 29.0 35.0 2.0 36.0 88-89 28.005853727013243 33.0 27.0 35.0 2.0 35.0 90-91 27.50453613151779 33.0 26.5 35.0 2.0 35.0 92-93 26.889808619156803 33.0 25.5 35.0 2.0 35.0 94-95 26.162965389234635 32.0 24.5 34.5 2.0 35.0 96-97 25.360088827538874 32.0 21.5 34.0 2.0 35.0 98-99 24.36800258917848 32.0 10.0 34.0 2.0 35.0 100-101 20.929541200692526 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08934944619657159 1101 2 0.05878885720264293 1101 3 0.17930914225230765 1101 4 0.03756399530685428 1101 5 0.02855843059522556 1101 6 0.05009425130923262 1101 7 0.06789714449192275 1101 8 0.19701650750840116 1101 9 0.11732579566083956 1101 10-11 0.21710261602249403 1101 12-13 0.07937947064836237 1101 14-15 0.26790993307461974 1101 16-17 0.33145319599790213 1101 18-19 0.23160167974840817 1101 20-21 0.2773705285412902 1101 22-23 0.1448206857814469 1101 24-25 0.25666252016804947 1101 26-27 0.24000246934309644 1101 28-29 0.2067314082421845 1101 30-31 0.2808086179635083 1101 32-33 0.20066332929912534 1101 34-35 0.24904342973794513 1101 36-37 0.2914074412679071 1101 38-39 0.32374762674277235 1101 40-41 0.22480620892194736 1101 42-43 0.41984313601245304 1101 44-45 0.4699029184733732 1101 46-47 0.47687071625429667 1101 48-49 0.5310643827904684 1101 50-51 0.6387589004897478 1101 52-53 0.42635511130946924 1101 54-55 0.3978795101224506 1101 56-57 0.4393824753976716 1101 58-59 0.7203254481388512 1101 60-61 0.6157128210254044 1101 62-63 0.6737434187470868 1101 64-65 0.47749663087770244 1101 66-67 0.548299145617456 1101 68-69 0.6263289748165093 1101 70-71 0.7777025170434122 1101 72-73 0.6250114422841442 1101 74-75 0.511572953153312 1101 76-77 0.42604019741897403 1101 78-79 0.6228128132930877 1101 80-81 0.5431781823257325 1101 82-83 0.8673503342224471 1101 84-85 0.8834723594601606 1101 86-87 0.8663802139554555 1101 88-89 1.1348473303349706 1101 90-91 1.1895518101313556 1101 92-93 1.4199935395205685 1101 94-95 1.4933279464716591 1101 96-97 1.6778593722353499 1101 98-99 1.898410699526405 1101 100-101 2.3170000597846148 1102 1 -0.017886772990458155 1102 2 0.12409440217734158 1102 3 0.2327825056957451 1102 4 0.067822833347158 1102 5 0.03403484132603296 1102 6 0.027587131902727435 1102 7 0.08116517881018837 1102 8 0.07325455869557373 1102 9 0.08584514049405101 1102 10-11 0.21643076171184106 1102 12-13 0.20027116497642083 1102 14-15 0.2446344619240861 1102 16-17 0.23913997838892698 1102 18-19 0.26404984865519054 1102 20-21 0.30992027369375563 1102 22-23 0.282033143382435 1102 24-25 0.1595291469297635 1102 26-27 0.2789594989634594 1102 28-29 0.12483846234966478 1102 30-31 0.187571056518955 1102 32-33 0.3413355463820693 1102 34-35 0.3242065865825481 1102 36-37 0.36092549825944076 1102 38-39 0.3817248083647229 1102 40-41 0.35739259407731083 1102 42-43 0.4564468616840358 1102 44-45 0.31935603922214284 1102 46-47 0.33496797550952095 1102 48-49 0.44560320849651447 1102 50-51 0.531150114144495 1102 52-53 0.4714825350819396 1102 54-55 0.4935512737400174 1102 56-57 0.6284260262130061 1102 58-59 0.7319261809389062 1102 60-61 0.8958260059848584 1102 62-63 0.7993863419663256 1102 64-65 0.9047324872924207 1102 66-67 0.9095586910637579 1102 68-69 0.9113704215524692 1102 70-71 1.0095902027374883 1102 72-73 1.1405950522313617 1102 74-75 1.2038270388764083 1102 76-77 1.375674341548411 1102 78-79 1.3804482634028261 1102 80-81 1.475997830073819 1102 82-83 1.4235901992711497 1102 84-85 1.363449867577188 1102 86-87 1.501411684452993 1102 88-89 1.8179526612740062 1102 90-91 2.064133080197198 1102 92-93 2.3182301033412855 1102 94-95 2.0835773524320054 1102 96-97 2.2214500925197633 1102 98-99 2.359281715833017 1102 100-101 2.962759432914069 1103 1 0.23208309886449996 1103 2 0.19949510650641855 1103 3 0.2763437863427569 1103 4 0.11850417576803807 1103 5 0.01873777781590036 1103 6 0.03952501882622528 1103 7 0.21237302730355623 1103 8 0.09020576895431986 1103 9 0.15193423946166007 1103 10-11 0.1477943838736664 1103 12-13 0.24969917656348173 1103 14-15 0.31530884139588977 1103 16-17 0.37263176344804805 1103 18-19 0.3659338619919197 1103 20-21 0.3798769337991743 1103 22-23 0.31133733945193853 1103 24-25 0.42931262599405073 1103 26-27 0.40557894255240967 1103 28-29 0.42856721084884697 1103 30-31 0.5457902376582879 1103 32-33 0.4571409944433782 1103 34-35 0.5153283801668209 1103 36-37 0.5646167539558888 1103 38-39 0.453900238974029 1103 40-41 0.5673415770850312 1103 42-43 0.6276742599959633 1103 44-45 0.5200978611045457 1103 46-47 0.6096344457853178 1103 48-49 0.8069226873465638 1103 50-51 0.8913644480000542 1103 52-53 0.7837283985992158 1103 54-55 1.0801074259072294 1103 56-57 1.0019891368658733 1103 58-59 1.2698988022660629 1103 60-61 1.2141465962286944 1103 62-63 1.2467343956881578 1103 64-65 1.143195639455179 1103 66-67 1.2920888303673763 1103 68-69 1.0461173228169613 1103 70-71 1.2263601977830838 1103 72-73 1.4792032658332133 1103 74-75 1.4775883257722313 1103 76-77 1.1127350843522485 1103 78-79 1.6434433134601818 1103 80-81 1.6648736262379522 1103 82-83 1.7036118915355836 1103 84-85 1.8447266760469923 1103 86-87 1.8130929242150025 1103 88-89 1.8448796351138483 1103 90-91 1.7976369165143353 1103 92-93 2.2037252984600784 1103 94-95 2.071213654391901 1103 96-97 2.04907470029595 1103 98-99 2.4347324105657258 1103 100-101 2.593263845013535 1104 1 0.13142349545105247 1104 2 0.15787413111787174 1104 3 0.12348069527626393 1104 4 -0.0037327043078221323 1104 5 -0.04162417955722475 1104 6 0.036346554877674464 1104 7 0.044462733367787166 1104 8 0.06765145473574563 1104 9 0.017737924995735455 1104 10-11 0.04421603535570995 1104 12-13 -0.033441772440930606 1104 14-15 0.042595810105950704 1104 16-17 0.04362776460235551 1104 18-19 0.1271285686745287 1104 20-21 0.11844105961852591 1104 22-23 0.16223398784209309 1104 24-25 0.19625206615565105 1104 26-27 0.23461798282944102 1104 28-29 0.11844023719175567 1104 30-31 0.17526832645739177 1104 32-33 0.09286470306357586 1104 34-35 0.07490705848647394 1104 36-37 -0.03250778741361415 1104 38-39 -0.09404262288028065 1104 40-41 -0.03789608626987473 1104 42-43 -0.03249393887446672 1104 44-45 -0.05915555028185793 1104 46-47 -0.1329986085907393 1104 48-49 -0.2235159489233851 1104 50-51 -0.13766428597364921 1104 52-53 -0.015288380062614237 1104 54-55 0.045051565743165156 1104 56-57 0.0964422844109123 1104 58-59 -0.09846496901645452 1104 60-61 -0.09311110257988986 1104 62-63 -0.09693341154274293 1104 64-65 -0.12507331383665843 1104 66-67 -0.12491244761423559 1104 68-69 0.09440235394199448 1104 70-71 0.1460472984138228 1104 72-73 0.04427932635182685 1104 74-75 -0.07221997698573901 1104 76-77 0.06994537416549562 1104 78-79 0.26844022156918257 1104 80-81 0.32319493555072754 1104 82-83 0.3984175146546818 1104 84-85 0.6522544000029633 1104 86-87 0.7153046569107211 1104 88-89 0.8026258527005261 1104 90-91 0.7177156566718175 1104 92-93 0.8845638192994265 1104 94-95 1.0703190672624139 1104 96-97 1.1217444451118759 1104 98-99 1.420000580578506 1104 100-101 2.138576538256718 1105 1 0.09102558717432174 1105 2 0.08991115702398389 1105 3 0.15382270405188336 1105 4 0.09762571367619444 1105 5 0.10900994919194318 1105 6 0.09555795116305887 1105 7 0.09459196339723519 1105 8 0.029964371727992045 1105 9 0.12118806624716427 1105 10-11 0.2004581463132169 1105 12-13 0.08708777014486913 1105 14-15 0.16439361019009624 1105 16-17 0.1475828045735028 1105 18-19 0.25113229530794 1105 20-21 0.22244117983250788 1105 22-23 0.16322575318440613 1105 24-25 0.15248516106459675 1105 26-27 0.2962847496744274 1105 28-29 0.18350905969581532 1105 30-31 0.16616512275480488 1105 32-33 0.20633167427232735 1105 34-35 0.2204320930479966 1105 36-37 0.32479220657568675 1105 38-39 0.17814587264174975 1105 40-41 0.2709065824805066 1105 42-43 0.2910710067287141 1105 44-45 0.4389300420725277 1105 46-47 0.5516831248948648 1105 48-49 0.6261805107748799 1105 50-51 0.5310830843455179 1105 52-53 0.5320218321150563 1105 54-55 0.43524511025238155 1105 56-57 0.5351872504504556 1105 58-59 0.6764193250269486 1105 60-61 0.653375643828717 1105 62-63 0.5624132254711469 1105 64-65 0.7429798207428959 1105 66-67 0.863850550522443 1105 68-69 0.8296731359339375 1105 70-71 1.0389336360948995 1105 72-73 0.942476116638538 1105 74-75 0.7068417528934461 1105 76-77 0.787370390694921 1105 78-79 0.8444304842367281 1105 80-81 1.1887295872307178 1105 82-83 1.1567152846853368 1105 84-85 1.1888775892798762 1105 86-87 1.2940868892843476 1105 88-89 1.1729600475673898 1105 90-91 1.4209193592624239 1105 92-93 1.634401530425226 1105 94-95 1.5490742514903353 1105 96-97 1.2729706277169157 1105 98-99 1.7761855898950891 1105 100-101 2.1497384063819815 1106 1 0.08534835755209258 1106 2 0.13142833977632762 1106 3 0.1704546203753523 1106 4 0.03906248241122512 1106 5 0.03560516226037436 1106 6 0.035696607904341704 1106 7 0.06911450516944484 1106 8 0.01816246591577908 1106 9 0.05063223307178788 1106 10-11 0.1036207147369268 1106 12-13 0.005971853338920141 1106 14-15 0.10558023389270232 1106 16-17 0.07962635450773092 1106 18-19 -0.0424773750520302 1106 20-21 0.02422866856018402 1106 22-23 0.10218232387119741 1106 24-25 0.1642497317973337 1106 26-27 0.07360828398536512 1106 28-29 -0.07908291383649413 1106 30-31 0.08578212227806858 1106 32-33 0.06548250224950891 1106 34-35 -0.20002465857033513 1106 36-37 -0.06407820623541483 1106 38-39 0.0682494918469132 1106 40-41 -0.05761282314503546 1106 42-43 -0.01964904335773099 1106 44-45 0.044052414975304544 1106 46-47 -0.24111465108317276 1106 48-49 -0.07507653263145642 1106 50-51 -0.14202876580869628 1106 52-53 -0.1849722344792326 1106 54-55 -0.26740970109058537 1106 56-57 -0.13481056688335258 1106 58-59 -0.07673233006584468 1106 60-61 -0.16314570322095534 1106 62-63 -0.05853232849527501 1106 64-65 -0.21804120752263856 1106 66-67 -0.18294495088767349 1106 68-69 -0.4050694530527892 1106 70-71 -0.20373289271917017 1106 72-73 -0.2501031055281615 1106 74-75 -0.06147541147854696 1106 76-77 0.07133325090663689 1106 78-79 0.19926767457096517 1106 80-81 0.3714675696430234 1106 82-83 0.16558897149571195 1106 84-85 0.2694403725238921 1106 86-87 0.37113759032225957 1106 88-89 0.22607637793114677 1106 90-91 0.3798792039484944 1106 92-93 0.4767093552835 1106 94-95 0.6792447292070385 1106 96-97 0.9967777719279702 1106 98-99 1.4624956778335623 1106 100-101 1.727359472556973 1107 1 -0.011528174777396316 1107 2 -0.047702820529501366 1107 3 0.03561399356318162 1107 4 -0.016936823023748104 1107 5 -0.05345805953848526 1107 6 -0.01435684957703387 1107 7 -0.02657711669449725 1107 8 0.004009065331949557 1107 9 -0.10867488370796963 1107 10-11 -0.1250667320922645 1107 12-13 -0.18390404230596147 1107 14-15 -0.0646982255271169 1107 16-17 -0.050792426705370985 1107 18-19 -0.05396636521345499 1107 20-21 -0.07212612093528037 1107 22-23 -0.01969063188523279 1107 24-25 0.025821338066329247 1107 26-27 0.036842477989608824 1107 28-29 -0.04970314074957827 1107 30-31 -0.17941094168198646 1107 32-33 -0.027104326569698856 1107 34-35 -0.15453993190241988 1107 36-37 -0.06402442112902662 1107 38-39 -2.612668124868378E-4 1107 40-41 -0.08246427250348631 1107 42-43 -0.04824197037052613 1107 44-45 -0.16105777077716255 1107 46-47 -0.1992037672710154 1107 48-49 -0.11192206071383737 1107 50-51 -0.005219112665301395 1107 52-53 -0.02672798784749375 1107 54-55 0.1100476230211953 1107 56-57 -0.0014235030478104704 1107 58-59 -0.01129824330418927 1107 60-61 0.18999747655453092 1107 62-63 0.277710314958064 1107 64-65 0.19430545031999458 1107 66-67 0.23343640899638984 1107 68-69 0.16904150917095961 1107 70-71 0.32455889230203994 1107 72-73 0.17100069590487266 1107 74-75 0.2944645938289767 1107 76-77 0.34914151601206456 1107 78-79 0.4376184211737666 1107 80-81 0.5148466503490745 1107 82-83 0.7019728213298819 1107 84-85 0.5718742987492895 1107 86-87 0.7411774841280732 1107 88-89 0.7751382237049462 1107 90-91 0.8249228381398801 1107 92-93 1.1662953027914185 1107 94-95 1.2129947469533953 1107 96-97 1.4464447411017325 1107 98-99 1.6189674731421881 1107 100-101 2.192698111231362 1108 1 -0.016608000611938678 1108 2 0.007450630561990579 1108 3 0.08501263260505709 1108 4 0.06004223867147118 1108 5 0.002960858666703814 1108 6 -0.02895619930862381 1108 7 -0.016570630911459716 1108 8 0.012657128153207964 1108 9 0.056712583731076904 1108 10-11 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quality scores pass #Quality Count 2 2.0 3 3.0 4 12.0 5 32.0 6 47.0 7 150.0 8 286.0 9 579.0 10 985.0 11 1628.0 12 2662.0 13 3769.0 14 5211.0 15 7293.0 16 10155.0 17 15088.0 18 15200.0 19 9949.0 20 8226.0 21 8648.0 22 8606.0 23 9225.0 24 10388.0 25 12011.0 26 14225.0 27 17034.0 28 21085.0 29 25606.0 30 30891.0 31 38310.0 32 48486.0 33 63767.0 34 89279.0 35 135703.0 36 198858.0 37 274279.0 38 182563.0 39 12019.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25085396097515 18.965030493035734 15.024488013351425 21.759627532637687 2 16.224310961670305 20.301408690185653 39.53660870765681 23.937671640487228 3 19.70158892083941 25.142353986262577 29.55745282879072 25.5986042641073 4 13.079328685290035 17.17374011510926 36.08519333052579 33.66173786907491 5 13.843502267489697 37.63876919957419 32.91934911542556 15.598379417510557 6 31.69518146893995 35.74655022624152 17.134617214320365 15.423651090498169 7 28.873551385834382 33.78152636750737 19.42562350849282 17.919298738165427 8 27.538451700047883 33.896454535670664 18.88247139031303 19.68262237396842 9 27.544101820223666 16.591798855146383 19.784131143449844 36.07996818118011 10-11 25.895255252444905 26.756235084928175 25.516080982016128 21.832428680610796 12-13 26.74574579258497 24.27846146647326 26.826345670924773 22.149447070017 14-15 23.51328903654485 25.269758083384026 25.96700357181851 25.249949308252617 16-17 22.196083477609847 28.835844524511412 26.35771996318999 22.610352034688752 18-19 21.957442328388936 26.328942648136884 29.83092352565002 21.882691497824155 20-21 23.14616380453262 25.337607037574283 29.98717108854678 21.52905806934631 22-23 22.820761779982217 25.344820863163477 29.97313337388673 21.86128398296757 24-25 22.305743789954587 25.798701800057245 30.185488330160492 21.710066079827676 26-27 21.949346461947446 26.133487677493513 30.021993262783802 21.895172597775243 28-29 21.659230559864366 26.076012842979218 30.35046796391051 21.91428863324591 30-31 21.940726391043295 25.803368591475813 30.26542261028795 21.99048240719294 32-33 21.78840312848883 25.990852558830746 30.0578724419784 22.162871870702023 34-35 22.407852365666336 26.068953898005514 29.80473995161691 21.71845378471124 36-37 22.446976444547744 25.8263360594494 29.67474570375443 22.05194179224843 38-39 21.973078604655335 25.94771494194774 29.7644719173255 22.314734536071416 40-41 22.16393498889011 25.869728943563615 29.936765068950766 22.029570998595517 42-43 22.273997808941065 25.833942069150567 29.54692504885812 22.345135073050248 44-45 22.040161012183546 26.173527207268478 29.495670906196597 22.29064087435138 46-47 22.281099566576266 26.398332595687602 28.944165742172345 22.37640209556379 48-49 22.436102902928194 26.44469868364922 28.737452788325584 22.381745625096997 50-51 22.414603902484675 26.63952708499056 28.430544507354206 22.515324505170558 52-53 22.669622385475645 26.660271707765975 28.23284669255845 22.43725921419993 54-55 22.32304680797966 26.567193860839456 28.454642584187294 22.65511674699359 56-57 22.328310950977183 26.90156442531156 28.2987069705052 22.471417653206057 58-59 22.570422535211268 26.374331258871052 28.29995476736387 22.755291438553805 60-61 22.433983747445915 26.567505810054122 28.573807184190414 22.42470325830955 62-63 22.236441907257497 26.62763402118135 28.492661394724937 22.643262676836212 64-65 22.0355466130114 26.410088437602358 28.538011011807278 23.016353937578963 66-67 22.096567149512815 26.750271786177198 28.23183789845725 22.92132316585274 68-69 22.101346139898414 26.51422216572471 28.447294969269077 22.9371367251078 70-71 22.123009374073902 26.652433983747446 28.281822719261307 22.94273392291735 72-73 22.26974248592329 26.725547080935225 27.952170386661052 23.052540046480434 74-75 22.082728931729914 27.03394787328623 27.709044967479297 23.174278227504562 76-77 22.092048414518118 26.805990984667694 28.04247968430739 23.059480916506793 78-79 22.196161464913512 26.86393555129225 27.96804080295728 22.97186218083696 80-81 22.071572179701747 27.353309552367776 27.815112091326395 22.760006176604083 82-83 22.313780173570166 27.17383844622881 27.68996325203861 22.822418128162415 84-85 22.300645877292283 26.88304836300152 27.60492727592488 23.211378483781317 86-87 22.21942765429037 26.78835125301277 27.797398244257455 23.194822848439404 88-89 22.10703921750598 27.033189082704823 27.576410070040453 23.283361629748747 90-91 22.10618960362469 27.095128592781272 27.710318111786208 23.088363691807835 92-93 22.237148259483245 26.751756777254954 27.63953321060621 23.37156175265559 94-95 22.023982674327105 26.791389259112236 27.66917604009798 23.515452026462672 96-97 22.171881299926614 26.7575454842516 27.442548568656672 23.628024647165116 98-99 22.01174724129339 26.734390920395533 27.387067428291566 23.86679441001951 100-101 22.157707479672005 26.522357282904206 27.42625691962858 23.893678317795203 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1677.0 1 1074.5 2 781.5 3 1013.5 4 741.5 5 665.0 6 1316.0 7 2738.5 8 4225.5 9 4783.5 10 3826.5 11 2662.5 12 2435.5 13 2465.5 14 2583.5 15 2642.5 16 2604.0 17 2632.0 18 2744.5 19 2795.0 20 2944.0 21 3343.0 22 3772.0 23 4309.5 24 4754.5 25 5453.0 26 6683.0 27 8077.0 28 9251.5 29 10649.0 30 12771.0 31 14879.5 32 17306.0 33 19629.5 34 21589.5 35 23372.0 36 25850.0 37 28558.5 38 29989.0 39 30968.5 40 32134.0 41 34911.0 42 37873.0 43 39783.0 44 41515.0 45 43929.5 46 44914.5 47 45325.5 48 47992.0 49 48842.0 50 48017.5 51 46231.0 52 44531.0 53 45043.5 54 45542.0 55 43387.0 56 39657.0 57 36625.0 58 33440.0 59 29289.5 60 25167.0 61 21086.0 62 17243.0 63 13651.5 64 10908.0 65 8991.0 66 6776.0 67 5502.5 68 4098.0 69 2448.5 70 1720.5 71 1380.0 72 1186.0 73 1022.0 74 801.0 75 643.5 76 433.0 77 217.5 78 112.0 79 74.0 80 38.5 81 24.5 82 18.5 83 7.0 84 3.0 85 1.5 86 0.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011464133639043564 3 0.005381123953020449 4 0.0 5 3.899365183348151E-4 6 1.5597460733392604E-4 7 0.0 8 1.5597460733392604E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017937079843401494 26-27 0.021563489463915276 28-29 2.3396191100088904E-4 30-31 2.3396191100088904E-4 32-33 0.0033924477095128914 34-35 0.015636454385226085 36-37 0.0073308065446945235 38-39 0.02093959103457957 40-41 0.02296726092992061 42-43 0.018054060798901938 44-45 0.009436463743702526 46-47 0.002144650850841483 48-49 2.3396191100088904E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.5597460733392604E-4 68-69 7.798730366696302E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5597460733392604E-4 82-83 9.358476440035562E-4 84-85 0.0039383588351816325 86-87 8.188666885031116E-4 88-89 7.798730366696302E-5 90-91 0.004172320746182521 92-93 8.968539921700747E-4 94-95 7.798730366696302E-5 96-97 2.3396191100088904E-4 98-99 1.1698095550044452E-4 100-101 0.0029635175393445947 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1282260.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.860567661549666 #Duplication Level Percentage of deduplicated Percentage of total 1 79.02977464681379 43.3561829928855 2 12.20761067479353 13.39432902820733 3 3.651712215459022 6.010050152300914 4 1.5947354416272708 3.4995236639065666 5 0.8460427073846397 2.3207191596517847 6 0.513508752649352 1.690282900171065 7 0.361641069734783 1.3887884062766187 8 0.2492074795390936 1.093733103441496 9 0.18819940014810482 0.9292253333119348 >10 1.1761497868295174 12.294455410841909 >50 0.11429622910215934 4.273541143858544 >100 0.06341637749927016 6.277623239388184 >500 0.002280134411934168 0.8550646959507583 >1k 0.001140067205967084 1.189600842423584 >5k 2.85016801491771E-4 1.4268799273838613 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9734 0.759128413894218 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8517 0.6642178653315239 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4996 0.38962456912014726 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2277 0.1775770904496748 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1796 0.14006519738586556 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1631 0.1271972922808167 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 7.798730366696302E-5 0.0 8 0.0 0.0 0.0 7.798730366696302E-5 0.0 9 0.0 0.0 0.0 7.798730366696302E-5 0.0 10-11 0.0 0.0 0.0 1.5597460733392604E-4 0.0 12-13 0.0 0.0 0.0 2.3396191100088904E-4 0.0 14-15 0.0 0.0 0.0 2.3396191100088904E-4 0.0 16-17 0.0 0.0 0.0 3.119492146678521E-4 0.0 18-19 0.0 0.0 0.0 3.509428665013336E-4 0.0 20-21 0.0 0.0 0.0 4.2893017016829656E-4 0.0 22-23 0.0 0.0 0.0 6.238984293357042E-4 0.0 24-25 7.798730366696302E-5 0.0 0.0 0.001364777814171853 0.0 26-27 7.798730366696302E-5 0.0 0.0 0.002222638154508446 0.0 28-29 7.798730366696302E-5 0.0 0.0 0.005498104908520892 0.0 30-31 7.798730366696302E-5 0.0 0.0 0.015090543259557346 0.0 32-33 7.798730366696302E-5 0.0 0.0 0.03376850248779499 0.0 34-35 7.798730366696302E-5 0.0 0.0 0.060947077815731596 0.0 36-37 7.798730366696302E-5 0.0 0.0 0.10660864411273845 0.0 38-39 7.798730366696302E-5 0.0 0.0 0.1744186046511628 0.0 40-41 7.798730366696302E-5 0.0 0.0 0.2473757272316067 0.0 42-43 7.798730366696302E-5 0.0 0.0 0.3192410275607131 0.0 44-45 7.798730366696302E-5 0.0 0.0 0.40724969974888087 0.0 46-47 7.798730366696302E-5 0.0 0.0 0.5110508009296086 0.0 48-49 7.798730366696302E-5 0.0 0.0 0.6215978038775287 0.0 50-51 7.798730366696302E-5 0.0 0.0 0.7404894483178139 0.0 52-53 7.798730366696302E-5 0.0 0.0 0.8573924165145914 0.0 54-55 7.798730366696302E-5 0.0 0.0 0.9777658197245489 0.0 56-57 7.798730366696302E-5 0.0 0.0 1.116817182162744 0.0 58-59 7.798730366696302E-5 0.0 0.0 1.2669817353734811 0.0 60-61 7.798730366696302E-5 0.0 0.0 1.4281424984012603 0.0 62-63 7.798730366696302E-5 0.0 0.0 1.5879384836148676 0.0 64-65 7.798730366696302E-5 0.0 0.0 1.7464476783179699 0.0 66-67 7.798730366696302E-5 0.0 0.0 1.924453698937813 0.0 68-69 7.798730366696302E-5 0.0 0.0 2.1273376694274173 0.0 70-71 7.798730366696302E-5 0.0 0.0 2.3461700435169153 0.0 72-73 7.798730366696302E-5 0.0 0.0 2.5640275763105764 0.0 74-75 7.798730366696302E-5 0.0 0.0 2.780832280504734 0.0 76-77 7.798730366696302E-5 0.0 0.0 3.0172897852229656 0.0 78-79 7.798730366696302E-5 0.0 0.0 3.274023988894608 0.0 80-81 7.798730366696302E-5 0.0 0.0 3.5578197869386865 0.0 82-83 7.798730366696302E-5 0.0 0.0 3.845514950166113 0.0 84-85 7.798730366696302E-5 0.0 0.0 4.127361065618517 0.0 86-87 7.798730366696302E-5 0.0 0.0 4.4153681780606115 0.0 88-89 7.798730366696302E-5 0.0 0.0 4.720103567139271 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4375 0.0 54.507664 1 GTATCAA 7690 0.0 42.37695 1 TATCAAC 9905 0.0 32.90043 2 ATCAACG 9985 0.0 32.63174 3 TCAACGC 10130 0.0 32.258427 4 CAACGCA 10285 0.0 31.818457 5 TAGACCG 60 0.003952432 31.664522 5 AACGCAG 10585 0.0 31.051275 6 ACGCAGA 11820 0.0 27.605997 7 CGCAGAG 11955 0.0 27.135344 8 GAGTACT 6750 0.0 25.648262 12-13 GCAGAGT 12515 0.0 25.503666 9 GTACATG 8620 0.0 24.523626 1 GTGGTAT 1925 0.0 24.183954 1 TGGTATC 1945 0.0 24.17951 2 TACATGG 8680 0.0 24.080465 2 GTACTTT 7125 0.0 23.798388 14-15 ACATGGG 8680 0.0 22.763435 3 CAGAGTA 12315 0.0 22.253872 10-11 AGTACTT 6890 0.0 21.921593 12-13 >>END_MODULE