FastQCFastQC Report
Thu 26 May 2016
SRR938253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287324
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100870.7835634230387998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85620.6651006273478938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49510.384596263256181No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22690.17625710388371538No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17600.13671771830557033No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16050.12467723743206838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43850.056.1695141
GTATCAA80400.043.1617431
TATCAAC101850.033.827332
ATCAACG102350.033.6620753
TCAACGC104400.032.910054
CAACGCA105250.032.6442685
AACGCAG107950.031.7837626
GTCGACC600.003934854631.6933698
ACGCAGA120900.028.2330537
CGCAGAG122550.027.8141338
GCAGAGT128300.026.1599989
GAGTACT68500.025.85106812-13
GTGGTAT19750.025.1354581
GTACATG87450.024.6717051
TGGTATC18950.024.074252
GTACTTT74100.023.91320814-15
TACATGG89750.023.7740212
AGTACTT71500.023.07098812-13
ACATGGG89100.022.6674143
CAGAGTA125800.022.57963810-11
Produced by FastQC (version 0.11.5)