FastQCFastQC Report
Thu 26 May 2016
SRR938253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287324
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97970.7610360717270866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87450.6793161628308025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49240.38249888916853875No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23550.18293762875546482No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17210.1336881779567537No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16340.1269299725632397No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG13100.10176148351153246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46800.054.0016631
TAAGACG405.389628E-447.4978455
GTATCAA80350.039.7305681
TATCACG550.00257834934.5492552
ATCAACG99400.032.0181353
TATCAAC100450.031.6859152
TCAACGC101550.031.4313664
CAACGCA103750.030.719095
AACGCAG106350.030.0574046
GTGGTAT21450.028.5695761
TGGTATC21100.028.1429062
ACGCAGA121550.026.1814547
CGCAGAG122700.025.8586488
GAGTACT69050.024.35081512-13
GCAGAGT129900.024.2425489
GTACATG89250.023.9522151
TACATGG89100.023.5660062
GAGTGCA8600.023.196629
CGCGGAA3950.022.84706582-83
GTAAGGT3750.022.7989654
Produced by FastQC (version 0.11.5)