##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938253_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1287324 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.515106531067545 34.0 31.0 34.0 31.0 34.0 2 32.73005785645261 34.0 31.0 34.0 31.0 34.0 3 32.77218400340551 34.0 31.0 34.0 31.0 34.0 4 36.23248226553688 37.0 35.0 37.0 35.0 37.0 5 36.20092455357004 37.0 35.0 37.0 35.0 37.0 6 36.24927757114759 37.0 37.0 37.0 35.0 37.0 7 36.21692130341701 37.0 37.0 37.0 35.0 37.0 8 36.21603030783237 37.0 37.0 37.0 35.0 37.0 9 37.91307316572984 39.0 38.0 39.0 35.0 39.0 10-11 37.90751512439759 39.0 38.0 39.0 35.0 39.0 12-13 37.85053995730678 39.0 38.0 39.0 35.0 39.0 14-15 39.15127970891555 40.5 38.5 41.0 36.0 41.0 16-17 39.104354070925424 40.0 38.5 41.0 36.0 41.0 18-19 39.15821269548303 40.0 39.0 41.0 36.0 41.0 20-21 39.11462265909748 40.0 39.0 41.0 36.0 41.0 22-23 38.966969465340505 40.0 38.0 41.0 35.0 41.0 24-25 38.914676880101666 40.0 38.0 41.0 35.0 41.0 26-27 38.866512626192005 40.0 38.0 41.0 35.0 41.0 28-29 38.803020063325164 40.0 38.0 41.0 35.0 41.0 30-31 38.5897944107311 40.0 38.0 41.0 35.0 41.0 32-33 38.388200251063445 40.0 38.0 41.0 34.0 41.0 34-35 38.17735045722755 40.0 38.0 41.0 34.0 41.0 36-37 38.03755659026011 40.0 37.0 41.0 34.0 41.0 38-39 38.01004214945111 40.0 37.0 41.0 33.5 41.0 40-41 37.92133759644037 40.0 37.0 41.0 33.0 41.0 42-43 37.73243837604208 40.0 37.0 41.0 33.0 41.0 44-45 37.47089543891049 40.0 37.0 41.0 33.0 41.0 46-47 37.04466552320939 40.0 36.5 41.0 31.5 41.0 48-49 36.637835541013764 40.0 36.0 41.0 31.0 41.0 50-51 36.31677534171662 39.5 35.5 41.0 30.0 41.0 52-53 35.90584965401096 39.0 35.0 41.0 29.0 41.0 54-55 35.67455745406751 39.0 35.0 41.0 28.0 41.0 56-57 35.4423730933316 39.0 35.0 40.0 28.0 41.0 58-59 35.11566202447868 38.0 35.0 40.0 27.5 41.0 60-61 34.76740587451178 38.0 34.0 40.0 26.5 41.0 62-63 34.41303238345591 37.0 34.0 40.0 26.0 41.0 64-65 34.03905077509624 36.5 34.0 40.0 26.0 41.0 66-67 33.63434147114479 36.0 33.5 39.0 25.0 41.0 68-69 33.19542516103172 35.5 33.0 39.0 23.5 40.0 70-71 32.70165008964332 35.0 32.5 38.5 22.5 40.0 72-73 32.307844023726744 35.0 32.0 37.5 22.0 39.5 74-75 31.867783867930683 35.0 32.0 37.0 20.5 39.0 76-77 30.5741794606486 34.0 30.0 35.5 19.5 38.0 78-79 31.28279127865246 35.0 31.5 36.0 20.0 38.5 80-81 31.126982018512823 35.0 32.0 36.0 19.5 37.0 82-83 30.775083428880375 35.0 31.0 35.0 17.5 37.0 84-85 30.422097311943226 34.0 31.0 35.0 13.5 36.5 86-87 29.993450366807423 34.0 30.5 35.0 7.0 36.0 88-89 29.556071354220073 34.0 30.0 35.0 4.5 36.0 90-91 29.23657369861822 34.0 30.0 35.0 2.0 35.0 92-93 28.850213310712764 34.0 29.0 35.0 2.0 35.0 94-95 28.461137988571643 34.0 29.0 35.0 2.0 35.0 96-97 27.90790003138293 34.0 29.0 35.0 2.0 35.0 98-99 27.250289359943572 33.5 27.0 35.0 2.0 35.0 100-101 24.874163380780594 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08184356058684727 1101 2 0.13630001398693992 1101 3 0.15849443006663932 1101 4 -0.005783695267197686 1101 5 0.01492670015504416 1101 6 0.06677127051269593 1101 7 0.11990341058271525 1101 8 0.07927250437710143 1101 9 0.018208185160112578 1101 10-11 0.13343811227220215 1101 12-13 0.16376998069555526 1101 14-15 0.17045541597678238 1101 16-17 0.13609474569919655 1101 18-19 0.16967383783922685 1101 20-21 0.20207001444033068 1101 22-23 0.2348481592343319 1101 24-25 0.2547930937422578 1101 26-27 0.2850995020115761 1101 28-29 0.12217004942930743 1101 30-31 0.22917606732631413 1101 32-33 0.3516673772141843 1101 34-35 0.3178036855081743 1101 36-37 0.1544729688471449 1101 38-39 0.21393691465623021 1101 40-41 0.3556675357350656 1101 42-43 0.2883052267989825 1101 44-45 0.264596234307632 1101 46-47 0.4566771369059808 1101 48-49 0.4618999204951919 1101 50-51 0.3116825118201447 1101 52-53 0.20324612419335608 1101 54-55 0.10456137170066171 1101 56-57 0.09496586510736904 1101 58-59 0.1931821391117694 1101 60-61 0.28728300281600383 1101 62-63 0.1264817991578795 1101 64-65 0.22021905318038648 1101 66-67 0.22006346133509425 1101 68-69 0.1834174675005329 1101 70-71 0.3078731732734994 1101 72-73 0.38582587623555753 1101 74-75 0.3076362831760804 1101 76-77 0.2740851004546876 1101 78-79 0.22446952207668502 1101 80-81 0.3750211242494217 1101 82-83 0.49041902619311273 1101 84-85 0.5455025339054025 1101 86-87 0.42694434515654933 1101 88-89 0.3920541522856489 1101 90-91 0.5231273125309102 1101 92-93 0.6798681294432036 1101 94-95 0.4704764497550684 1101 96-97 0.3045427620983574 1101 98-99 0.6763020038954366 1101 100-101 0.8146555439969738 1102 1 0.14210449163091 1102 2 0.12502244844182542 1102 3 0.13954268143765347 1102 4 0.09085500960232906 1102 5 0.14324694920955494 1102 6 0.08218431089144929 1102 7 0.05415399081327621 1102 8 0.13154396555861325 1102 9 0.13141598193022475 1102 10-11 0.19757876104273464 1102 12-13 0.18425296476365816 1102 14-15 0.23780001046213073 1102 16-17 0.21462540641975636 1102 18-19 0.35028980090436335 1102 20-21 0.40050188530356223 1102 22-23 0.24443206887857372 1102 24-25 0.15065071052697476 1102 26-27 0.30771783744437187 1102 28-29 0.2316054288684981 1102 30-31 0.274733904444318 1102 32-33 0.24913229512937818 1102 34-35 0.24452229242567114 1102 36-37 0.4706442408565934 1102 38-39 0.3579601356377182 1102 40-41 0.5409944271250211 1102 42-43 0.4505614307461627 1102 44-45 0.5645864596825518 1102 46-47 0.578860685256565 1102 48-49 0.4205650153825431 1102 50-51 0.371275020194723 1102 52-53 0.30429369942488904 1102 54-55 0.2135760013011705 1102 56-57 0.37933249145063996 1102 58-59 0.3913479637888031 1102 60-61 0.3763640116748945 1102 62-63 0.2854039740340042 1102 64-65 0.439600958806011 1102 66-67 0.2806903896187407 1102 68-69 0.3974394978885911 1102 70-71 0.4571155295901832 1102 72-73 0.23764145772285872 1102 74-75 0.4163598788270306 1102 76-77 0.4591172832240069 1102 78-79 0.3067788470690047 1102 80-81 0.3892479808034963 1102 82-83 0.47173699842142014 1102 84-85 0.28447888813193245 1102 86-87 0.24562064044135568 1102 88-89 0.15001091757558882 1102 90-91 0.14434662719005686 1102 92-93 0.3378929496415459 1102 94-95 0.09043645463503225 1102 96-97 0.04644305210339894 1102 98-99 0.061129727965852254 1102 100-101 -0.010215879261927796 1103 1 0.09765363230545887 1103 2 0.07751567252626046 1103 3 0.07247747053817477 1103 4 -0.048538300656396416 1103 5 0.049388075959704736 1103 6 -0.04404111308320324 1103 7 -0.059974326399114375 1103 8 0.010786200629553377 1103 9 -0.04827305118288905 1103 10-11 0.027163418037787324 1103 12-13 0.09517420487706829 1103 14-15 0.18853775337834122 1103 16-17 0.1962842188072358 1103 18-19 0.10173741518484292 1103 20-21 0.08136337222241963 1103 22-23 0.1526728051425934 1103 24-25 0.1765925841209537 1103 26-27 0.17837179506255296 1103 28-29 0.14140256378671268 1103 30-31 0.19867585198323212 1103 32-33 0.19091772399576712 1103 34-35 0.21088872370096823 1103 36-37 0.18550902116903245 1103 38-39 0.20693898454262438 1103 40-41 0.1473969207757122 1103 42-43 0.20247120086274606 1103 44-45 0.26926248133136 1103 46-47 0.2601438004997476 1103 48-49 0.2586689819594383 1103 50-51 0.24841053566201055 1103 52-53 0.20002209968313167 1103 54-55 0.34684539122702773 1103 56-57 0.2508929855165647 1103 58-59 0.5019311902337833 1103 60-61 0.3754389889937073 1103 62-63 0.40368884973541697 1103 64-65 0.29529528751399425 1103 66-67 0.243316913666483 1103 68-69 0.1798956357447068 1103 70-71 0.3094986894847267 1103 72-73 0.5043416366127147 1103 74-75 0.4080268535975904 1103 76-77 0.206465930742727 1103 78-79 0.181515418242423 1103 80-81 0.287981105005386 1103 82-83 0.4098472218773246 1103 84-85 0.500136443455542 1103 86-87 0.5967502915065914 1103 88-89 0.45398005409784403 1103 90-91 0.42121565649215853 1103 92-93 0.3420175127265317 1103 94-95 0.22879524682985775 1103 96-97 0.4023528165319057 1103 98-99 0.52258593123479 1103 100-101 0.47363923986051404 1104 1 0.09927265287763731 1104 2 0.046965285155685876 1104 3 0.05909400949685306 1104 4 -0.02438835281009233 1104 5 0.021187922550865324 1104 6 -0.003478012419932952 1104 7 0.02510785880091504 1104 8 0.006474563056599436 1104 9 0.042834013851312136 1104 10-11 0.0685632338410187 1104 12-13 0.0942233620574271 1104 14-15 0.1938360645540982 1104 16-17 0.1475311300610258 1104 18-19 0.16354087139563944 1104 20-21 0.14978822445362283 1104 22-23 0.21263827488416354 1104 24-25 0.11311038936604945 1104 26-27 0.1208355197122728 1104 28-29 0.13922020367937904 1104 30-31 0.23863580113005867 1104 32-33 0.21924859340981584 1104 34-35 0.2516561925348171 1104 36-37 0.2645059450299456 1104 38-39 0.31087612407831244 1104 40-41 0.35311339046153734 1104 42-43 0.36153731794712485 1104 44-45 0.4377633744468099 1104 46-47 0.5749815932173377 1104 48-49 0.6287684588300024 1104 50-51 0.6062346516369601 1104 52-53 0.5145792179326776 1104 54-55 0.5482727834626289 1104 56-57 0.5633175086353788 1104 58-59 0.577496984841126 1104 60-61 0.5873110545189277 1104 62-63 0.5147111948734491 1104 64-65 0.573286428021099 1104 66-67 0.4414956482293917 1104 68-69 0.6785115058620974 1104 70-71 0.7158934226250224 1104 72-73 0.7428273480926393 1104 74-75 0.7717331898071222 1104 76-77 1.0371243412891893 1104 78-79 0.7213419972519581 1104 80-81 0.7343497214859553 1104 82-83 0.7119962726269833 1104 84-85 0.6924694521000525 1104 86-87 0.8064605664481341 1104 88-89 0.7959112271446038 1104 90-91 0.790739186700165 1104 92-93 1.0199631348683624 1104 94-95 0.9659439392716855 1104 96-97 0.9616494449879163 1104 98-99 1.098002430912313 1104 100-101 1.0144697443314037 1105 1 0.11613119286979412 1105 2 0.09045616241208876 1105 3 0.0786071762735645 1105 4 0.0478288077106086 1105 5 0.1201511549247769 1105 6 0.07155177374674082 1105 7 0.058524925382990034 1105 8 0.0836105908545619 1105 9 0.02524080989260824 1105 10-11 0.041602603222685275 1105 12-13 0.11268986322490093 1105 14-15 0.1541307047654854 1105 16-17 0.11908672414288901 1105 18-19 0.05886078527704086 1105 20-21 0.16376159457548312 1105 22-23 -0.011880258935583754 1105 24-25 0.02533894793690905 1105 26-27 0.12550816697397238 1105 28-29 -0.021504291800127362 1105 30-31 0.15508360556066947 1105 32-33 0.15473987150379998 1105 34-35 0.20659792968618973 1105 36-37 0.14391869338633256 1105 38-39 0.24988210836203706 1105 40-41 0.27713622590787423 1105 42-43 0.0972248957938433 1105 44-45 0.15512277697749255 1105 46-47 0.21346636569283106 1105 48-49 0.22899752643998994 1105 50-51 -0.041921249589769616 1105 52-53 0.14508166660763777 1105 54-55 0.25664930520068907 1105 56-57 0.24164904065873571 1105 58-59 0.19832031260452965 1105 60-61 0.330431648840559 1105 62-63 0.5106530217829217 1105 64-65 0.4671529982900111 1105 66-67 0.4133909091242458 1105 68-69 0.31355872723387535 1105 70-71 0.19414337313509833 1105 72-73 0.2810421699842678 1105 74-75 0.3142081405323722 1105 76-77 0.31927833915220916 1105 78-79 0.34179737825053635 1105 80-81 0.3884324243607935 1105 82-83 0.27147177788520693 1105 84-85 0.3637703421807963 1105 86-87 0.2560693809402572 1105 88-89 0.26097534271370293 1105 90-91 0.26755823312477744 1105 92-93 0.3815094088617492 1105 94-95 0.33003448675925995 1105 96-97 0.4774886771503368 1105 98-99 0.3313707453165229 1105 100-101 0.3506971618676751 1106 1 -0.029338005144786905 1106 2 0.04890611062592143 1106 3 0.09700204740489227 1106 4 0.07484151112565485 1106 5 0.0764085365950109 1106 6 -0.047858909742011235 1106 7 0.0275442141784481 1106 8 -0.056199646676432735 1106 9 -0.01765160545783573 1106 10-11 0.05743445800635527 1106 12-13 -0.03258583828117878 1106 14-15 -0.14763049750069968 1106 16-17 0.046293337646758914 1106 18-19 0.061523651731363316 1106 20-21 0.10435432607854267 1106 22-23 0.19589865520336502 1106 24-25 0.07554819003914304 1106 26-27 0.10331131101159485 1106 28-29 0.15599079397937743 1106 30-31 0.16450762406071107 1106 32-33 0.08294173651472647 1106 34-35 0.05361456802260278 1106 36-37 0.11348804401205115 1106 38-39 0.11755235722512936 1106 40-41 0.2488972568336365 1106 42-43 0.20074818656976134 1106 44-45 0.2297492105502883 1106 46-47 0.07351048880506283 1106 48-49 0.121356157300049 1106 50-51 0.0749491644691247 1106 52-53 -0.10897806738931592 1106 54-55 -0.43529214127877225 1106 56-57 -0.12049311635171023 1106 58-59 -0.05359648448764176 1106 60-61 -0.16969862896134202 1106 62-63 -0.2881504903411809 1106 64-65 -0.441703351674569 1106 66-67 -0.45926416429512784 1106 68-69 -0.5250480506386381 1106 70-71 -0.5404039366135223 1106 72-73 -0.43364324014265776 1106 74-75 -0.4342162661423359 1106 76-77 -0.37696316103361127 1106 78-79 -0.21035746385927112 1106 80-81 -0.5814163883978054 1106 82-83 -0.7901974795667712 1106 84-85 -0.5243352058810764 1106 86-87 -0.24168842287732772 1106 88-89 -0.3650792132576015 1106 90-91 -0.4187254345291649 1106 92-93 -0.25197954883902796 1106 94-95 -0.35814810540859554 1106 96-97 -0.23792522517498682 1106 98-99 -0.18704598648904636 1106 100-101 -0.14943193427615853 1107 1 0.09871419412872484 1107 2 0.0854811156789026 1107 3 0.08746133752126184 1107 4 0.05152757894862958 1107 5 -7.69011962120203E-4 1107 6 -0.021819223586255987 1107 7 0.04147901761470507 1107 8 0.11044532358236125 1107 9 0.03974110295043687 1107 10-11 -0.0536619985134692 1107 12-13 0.060701578601594974 1107 14-15 0.2374059467549472 1107 16-17 -0.002475048631524146 1107 18-19 0.004014753349338207 1107 20-21 0.06256647355473177 1107 22-23 0.09825225869123955 1107 24-25 0.05301903977325395 1107 26-27 0.0035674999119379436 1107 28-29 0.033724969590103626 1107 30-31 0.019275580978494133 1107 32-33 0.0046899302887766225 1107 34-35 0.0891892544809636 1107 36-37 0.1525361540836343 1107 38-39 0.1834637605145346 1107 40-41 0.2709742082827873 1107 42-43 0.20340199982763352 1107 44-45 0.1988386808267819 1107 46-47 0.2786942745084957 1107 48-49 0.09140340979256933 1107 50-51 0.17093150359311693 1107 52-53 0.1032333561120069 1107 54-55 0.13714674051120568 1107 56-57 0.15677498616038577 1107 58-59 0.2137977540883398 1107 60-61 0.23200236941599428 1107 62-63 0.35328097013537985 1107 64-65 0.38273673826275 1107 66-67 0.37551968809621883 1107 68-69 0.5926538201626812 1107 70-71 0.41480811151595987 1107 72-73 0.36946499866855476 1107 74-75 0.4764867431494366 1107 76-77 0.4542930267248195 1107 78-79 0.43642644334405034 1107 80-81 0.6414245620875683 1107 82-83 0.3981941428791913 1107 84-85 0.4028978968993755 1107 86-87 0.4844455284610554 1107 88-89 0.6596466983055542 1107 90-91 0.6475545222150529 1107 92-93 0.6565389747546746 1107 94-95 0.4838603653258069 1107 96-97 0.7265097946543868 1107 98-99 0.7087139073584261 1107 100-101 0.8104736749304244 1108 1 0.04641391178360266 1108 2 0.05364255065647683 1108 3 0.06927831144585639 1108 4 0.05928708733593879 1108 5 -0.0011343997714519105 1108 6 0.018283749341826194 1108 7 0.0524340084800059 1108 8 -0.017021874557485717 1108 9 0.04527174570080916 1108 10-11 0.05906844493446073 1108 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50-51 -0.49400351362669426 2316 52-53 -0.40493614249281507 2316 54-55 -0.6347879692465526 2316 56-57 -0.7176876804057315 2316 58-59 -0.6151721320346795 2316 60-61 -0.42048381896348985 2316 62-63 -0.47466597622505446 2316 64-65 -0.5984727864860062 2316 66-67 -0.5194312630057141 2316 68-69 -0.3563263340324383 2316 70-71 -0.419079233616948 2316 72-73 -0.3195347248089675 2316 74-75 -0.22900619199154448 2316 76-77 -0.1883490584479688 2316 78-79 -0.2816859717413145 2316 80-81 -0.2651340481066349 2316 82-83 -0.2504371675859254 2316 84-85 -0.38884249297796813 2316 86-87 -0.3948214742867613 2316 88-89 -0.46971554032753104 2316 90-91 -0.6231624251315893 2316 92-93 -0.8468812987898602 2316 94-95 -0.668338181674315 2316 96-97 -0.50796288859976 2316 98-99 -0.5040188887865078 2316 100-101 -0.3731164568915055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 1.0 4 12.0 5 31.0 6 50.0 7 113.0 8 303.0 9 527.0 10 917.0 11 1587.0 12 2342.0 13 3536.0 14 4752.0 15 6587.0 16 9252.0 17 13928.0 18 13919.0 19 8724.0 20 6892.0 21 6828.0 22 6600.0 23 7183.0 24 8023.0 25 9287.0 26 11010.0 27 13354.0 28 16532.0 29 20433.0 30 24867.0 31 30904.0 32 38926.0 33 51132.0 34 73383.0 35 114694.0 36 182628.0 37 297884.0 38 269417.0 39 30760.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.144908352520424 19.083851462413502 15.065049668925617 21.70619051614046 2 16.23669330465981 20.323058826819896 39.45572100496141 23.984526863558884 3 19.479485342900162 25.171725866320287 29.64724143235143 25.701547358428122 4 13.069204023229583 17.22713163119774 36.1019448095429 33.601719536029776 5 13.761570513716826 37.656021327964055 33.01391102783759 15.568497130481527 6 31.763176946906917 35.7421286327296 17.132206033601484 15.362488386761996 7 28.900261317275216 33.787686705134064 19.458504618883822 17.853547358706898 8 27.55946064776334 33.944343373297436 18.900632475790825 19.595563503148398 9 27.569157295122654 16.537548191240866 19.800841904764866 36.09245260887162 10-11 25.881751602549162 26.72703220012988 25.568737940098995 21.82247825722196 12-13 26.719031106388137 24.261141717236686 26.881655278702176 22.138171897673004 14-15 23.534828838738346 25.230594628858004 25.941099521177264 25.29347701122639 16-17 22.259603975240005 28.827830874799048 26.404149400478595 22.508415749482356 18-19 22.01295089658858 26.27928167267914 29.867344972982714 21.840422457749565 20-21 23.146465070176582 25.335579854022765 30.024259626947064 21.493695448853593 22-23 22.79029987788622 25.315149876798692 30.055603717479052 21.83894652783604 24-25 22.34826047658463 25.740301033937495 30.153500040978404 21.757938448499473 26-27 21.994705771749924 26.16051130403974 30.009082701532634 21.835700222677694 28-29 21.642928137333 26.122532525818105 30.37771779976051 21.856821537088386 30-31 21.941640177608747 25.770357734338827 30.29750086225379 21.990501225798635 32-33 21.766219057336535 25.944859885319932 30.129326768275654 22.159594289067876 34-35 22.40327622013328 26.07329074122849 29.84085066114104 21.682582377497198 36-37 22.447360928717554 25.804243907990525 29.756995464469476 21.991399698822438 38-39 21.968951528474694 25.93343337607746 29.784327992065982 22.313287103381864 40-41 22.149342254514718 25.81860799736467 29.967120494995044 22.064929253125573 42-43 22.314129520278865 25.864047289250458 29.547546780793134 22.274276409677547 44-45 22.04871394607511 26.115006674872532 29.456870846168755 22.379408532883605 46-47 22.285376486416784 26.440119192992594 28.938557814505128 22.33594650608549 48-49 22.448156019774355 26.439070505948774 28.706331894690067 22.406441579586804 50-51 22.42011335141736 26.65878986175974 28.384074250149922 22.537022536672975 52-53 22.66542843914974 26.670170058198245 28.25869788802197 22.40570361463004 54-55 22.278152197892684 26.614589644875725 28.404310026069584 22.702948131162007 56-57 22.29232889311471 26.92263175393296 28.281846683507805 22.503192669444523 58-59 22.542654374500902 26.43165201612026 28.33668913187356 22.689004477505275 60-61 22.421239718982942 26.570272907209063 28.626981241707604 22.381506132100387 62-63 22.215774738915766 26.66974281532854 28.555010238292766 22.55947220746292 64-65 22.014698708328282 26.45410168691021 28.584451156041524 22.94674844871998 66-67 21.99900724293185 26.871440290090142 28.252211564454637 22.877340902523375 68-69 22.092616335546325 26.53661109520815 28.4479440450579 22.922828524187626 70-71 22.092534591136342 26.7300617404787 28.308607623255682 22.868796045129276 72-73 22.19196565899494 26.833648716251695 27.93772197209094 23.036663652662423 74-75 22.13129717149684 27.039230217101522 27.737772309069047 23.09170030233259 76-77 22.07070636452051 26.831706703207587 28.017849430291054 23.079737501980855 78-79 22.236554278487777 26.916999916105038 27.899619676165443 22.946826129241746 80-81 22.011436126414175 27.490592888814312 27.757697362901645 22.740273621869864 82-83 22.29656248155088 27.258444649521024 27.62501903172783 22.81997383720027 84-85 22.244391991383438 27.002338636599333 27.56204223062662 23.191227141390605 86-87 22.244633131091387 26.85432792713852 27.699650319908926 23.201388621861163 88-89 22.154316420322026 27.096784216853198 27.546127718578678 23.202771644246102 90-91 22.124456818107667 27.242795508815977 27.671631495461117 22.961116177615235 92-93 22.1980721173229 26.856513420527467 27.646954639836746 23.298459822312882 94-95 22.047208006686738 26.817025084594086 27.680832486615646 23.454934422103527 96-97 22.191887978473172 26.786962722671216 27.57441017179824 23.44673912705737 98-99 21.97383875388014 26.78672968110592 27.44499442253854 23.7944371424754 100-101 21.983781860666003 26.740937013525734 27.596782162066425 23.67849896374184 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1595.0 1 1021.5 2 812.0 3 1091.5 4 805.5 5 708.5 6 1372.0 7 2753.0 8 4226.0 9 4866.0 10 3866.5 11 2637.5 12 2460.5 13 2558.0 14 2645.5 15 2652.5 16 2647.0 17 2700.0 18 2801.0 19 2903.0 20 3020.0 21 3314.0 22 3734.5 23 4282.0 24 4852.5 25 5549.0 26 6671.5 27 8036.5 28 9317.0 29 10805.5 30 12743.0 31 14882.0 32 17349.5 33 19689.0 34 21726.0 35 23623.5 36 26195.5 37 28741.5 38 30201.5 39 31093.0 40 31966.0 41 34664.0 42 37699.5 43 40155.0 44 42159.5 45 44306.0 46 45235.0 47 45582.0 48 48365.0 49 49265.5 50 48214.5 51 46536.5 52 44626.0 53 44930.5 54 45860.5 55 43691.5 56 39693.5 57 36603.5 58 33647.5 59 29496.0 60 25096.0 61 20936.0 62 17001.5 63 13431.0 64 10811.0 65 8965.0 66 6752.0 67 5519.0 68 4014.0 69 2302.5 70 1694.0 71 1383.5 72 1198.5 73 996.5 74 756.5 75 661.5 76 453.5 77 227.0 78 120.0 79 70.0 80 34.5 81 20.5 82 13.0 83 7.0 84 3.5 85 2.5 86 1.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014759299135260433 3 0.007612691132923802 4 0.0 5 0.0 6 0.0 7 0.0 8 1.5536104352905717E-4 9 3.884026088226429E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.272428697049072E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00469967156675398 26-27 0.02035229670230649 28-29 3.884026088226429E-5 30-31 0.0 32-33 2.7188182617585003E-4 34-35 0.017944200527606105 36-37 0.003379102696756994 38-39 0.016118708266139682 40-41 0.015536104352905717 42-43 0.0075350106111592725 44-45 0.0026022974791117077 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.9420130441132145E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.002757658522640765 86-87 3.4956234794037864E-4 88-89 1.1652078264679289E-4 90-91 1.5536104352905717E-4 92-93 3.884026088226429E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1287324.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.62887861050185 #Duplication Level Percentage of deduplicated Percentage of total 1 79.05812655969824 43.18856799003447 2 12.154927976975989 13.280201699472283 3 3.6029502418109005 5.904753941986979 4 1.6483545769199293 3.601910483584958 5 0.8333303459836131 2.2761951156593154 6 0.5305489374936921 1.7389976101964184 7 0.3567388466360261 1.3641770203970935 8 0.2626162683789039 1.1477145797131292 9 0.19056572304465513 0.9369352576376081 >10 1.179408672797162 12.375813161378325 >50 0.11307812688554761 4.208674650000268 >100 0.06536624869993686 6.393123082779792 >500 0.002278556957364991 0.7845037595439187 >1k 0.0014240980983531194 1.3559218588943829 >5k 2.8481961967062385E-4 1.442509788721077 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9797 0.7610360717270866 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8745 0.6793161628308025 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4924 0.38249888916853875 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2355 0.18293762875546482 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1721 0.1336881779567537 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1634 0.1269299725632397 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 1310 0.10176148351153246 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.768052176452858E-5 0.0 12-13 0.0 0.0 0.0 7.768052176452858E-5 0.0 14-15 0.0 0.0 0.0 7.768052176452858E-5 0.0 16-17 0.0 0.0 0.0 7.768052176452858E-5 0.0 18-19 0.0 0.0 0.0 7.768052176452858E-5 0.0 20-21 0.0 0.0 0.0 2.7188182617585003E-4 0.0 22-23 0.0 0.0 0.0 5.437636523517001E-4 0.0 24-25 0.0 0.0 0.0 0.0012817286091147215 0.0 26-27 0.0 0.0 0.0 0.002679978000876236 0.0 28-29 0.0 0.0 0.0 0.005631837827928322 0.0 30-31 0.0 0.0 0.0 0.014759299135260431 0.0 32-33 0.0 0.0 0.0 0.0320820554887503 0.0 34-35 0.0 0.0 0.0 0.060513126454567766 0.0 36-37 0.0 0.0 0.0 0.10502406542564266 0.0 38-39 0.0 0.0 0.0 0.1791701234498852 0.0 40-41 0.0 0.0 0.0 0.25762745043205904 0.0 42-43 0.0 0.0 0.0 0.33262799419571143 0.0 44-45 0.0 0.0 0.0 0.41916409544139627 0.0 46-47 0.0 0.0 0.0 0.5215858633879272 0.0 48-49 0.0 0.0 0.0 0.6301055522929737 0.0 50-51 0.0 0.0 0.0 0.7479080635488813 0.0 52-53 0.0 0.0 0.0 0.8637297214997934 0.0 54-55 0.0 0.0 0.0 0.9853774185830452 0.0 56-57 0.0 0.0 0.0 1.1272220513250744 0.0 58-59 0.0 0.0 0.0 1.2799031168532553 0.0 60-61 0.0 0.0 0.0 1.44637247499464 0.0 62-63 0.0 0.0 0.0 1.604957260176925 0.0 64-65 0.0 0.0 0.0 1.7656394194468525 0.0 66-67 0.0 0.0 0.0 1.9448095428967376 0.0 68-69 0.0 0.0 0.0 2.144720365657752 0.0 70-71 0.0 0.0 0.0 2.365915651382247 0.0 72-73 0.0 0.0 0.0 2.5873439786720356 0.0 74-75 0.0 0.0 0.0 2.8024413434380158 0.0 76-77 0.0 0.0 0.0 3.028685863077205 0.0 78-79 0.0 0.0 0.0 3.282273926377509 0.0 80-81 0.0 0.0 0.0 3.559554548815994 0.0 82-83 0.0 0.0 0.0 3.8441759805612263 0.0 84-85 0.0 0.0 0.0 4.125767871957642 0.0 86-87 0.0 0.0 0.0 4.412331316746988 0.0 88-89 0.0 0.0 0.0 4.72351948693569 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4680 0.0 54.001663 1 TAAGACG 40 5.389628E-4 47.497845 5 GTATCAA 8035 0.0 39.730568 1 TATCACG 55 0.002578349 34.549255 2 ATCAACG 9940 0.0 32.018135 3 TATCAAC 10045 0.0 31.685915 2 TCAACGC 10155 0.0 31.431366 4 CAACGCA 10375 0.0 30.71909 5 AACGCAG 10635 0.0 30.057404 6 GTGGTAT 2145 0.0 28.569576 1 TGGTATC 2110 0.0 28.142906 2 ACGCAGA 12155 0.0 26.181454 7 CGCAGAG 12270 0.0 25.858648 8 GAGTACT 6905 0.0 24.350815 12-13 GCAGAGT 12990 0.0 24.242548 9 GTACATG 8925 0.0 23.952215 1 TACATGG 8910 0.0 23.566006 2 GAGTGCA 860 0.0 23.19662 9 CGCGGAA 395 0.0 22.847065 82-83 GTAAGGT 375 0.0 22.798965 4 >>END_MODULE