FastQCFastQC Report
Thu 26 May 2016
SRR938252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321781
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100320.758975957439243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86150.6517721165609128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48030.36337335761370454No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.17597468869653898No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18010.13625555216787047No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15320.11590422316556223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43600.056.032641
GTATCAA77700.042.3513451
TGGTATC18100.034.1636282
GTGGTAT18750.033.27211
TATCAAC102350.032.0206832
ATCAACG102400.031.9585973
TCAACGC103550.031.6036724
CAACGCA105100.031.1828465
AACGCAG108100.030.3105566
ACGCAGA123150.026.6023067
AACCGAT908.895369E-426.4156937
CGCAGAG124750.026.1809168
GTACATG91000.025.1194461
GCAGAGT128950.024.9954269
TACATGG92250.024.3892
GAGTACT69400.024.38057512-13
ACATGGG92800.023.321833
GTACTTT73950.022.68853414-15
GTACACG1703.0800584E-622.4104841
CAGAGTA126500.021.99958410-11
Produced by FastQC (version 0.11.5)