##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938252_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1321781 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.924437558112878 33.0 31.0 34.0 30.0 34.0 2 32.02229643185974 33.0 31.0 34.0 30.0 34.0 3 32.02648925956721 34.0 31.0 34.0 30.0 34.0 4 35.569541399066864 37.0 35.0 37.0 33.0 37.0 5 35.579695123473556 37.0 35.0 37.0 33.0 37.0 6 35.622211243768824 37.0 35.0 37.0 33.0 37.0 7 35.558129523725945 37.0 35.0 37.0 33.0 37.0 8 35.55425142289078 37.0 35.0 37.0 33.0 37.0 9 37.16353919446565 39.0 37.0 39.0 34.0 39.0 10-11 37.06812739780644 39.0 37.0 39.0 33.0 39.0 12-13 36.90165806589745 39.0 37.0 39.0 33.0 39.0 14-15 38.03042864135587 40.0 38.0 41.0 33.0 41.0 16-17 37.850376499586545 40.0 38.0 41.0 33.0 41.0 18-19 38.06563000981252 40.0 38.0 41.0 33.0 41.0 20-21 38.086141728470906 40.0 38.0 41.0 33.0 41.0 22-23 37.96087627224178 40.0 38.0 41.0 33.0 41.0 24-25 37.8244039670717 40.0 38.0 41.0 32.5 41.0 26-27 37.6352894314565 40.0 37.5 41.0 32.0 41.0 28-29 37.44597365221621 40.0 37.0 41.0 32.0 41.0 30-31 37.18933923244471 40.0 37.0 41.0 31.0 41.0 32-33 36.86250142799753 39.0 36.0 41.0 30.5 41.0 34-35 36.594977155822335 39.0 35.0 41.0 30.0 41.0 36-37 36.318488085393874 39.0 35.0 41.0 30.0 41.0 38-39 36.01063489337493 39.0 35.0 40.0 29.5 41.0 40-41 35.847519369698915 39.0 35.0 40.0 29.0 41.0 42-43 35.578851186391695 38.5 35.0 40.0 27.5 41.0 44-45 35.171180399778784 38.0 35.0 40.0 25.5 41.0 46-47 34.67173608941269 38.0 34.0 40.0 24.0 41.0 48-49 34.182008214673985 38.0 33.5 40.0 22.5 41.0 50-51 33.419716655028324 37.5 32.5 39.5 20.0 40.5 52-53 33.473218710209935 38.0 33.0 39.5 20.0 40.5 54-55 33.527317687271946 38.0 33.0 40.0 18.5 41.0 56-57 33.201519011091854 37.5 33.0 40.0 15.5 41.0 58-59 32.79151690030345 37.0 32.5 40.0 11.0 41.0 60-61 32.27588042194584 36.0 31.5 39.5 7.5 41.0 62-63 31.715020113014184 35.5 31.0 39.0 4.5 40.5 64-65 31.162560968874573 35.0 30.5 39.0 2.0 40.0 66-67 30.58434718005479 35.0 29.5 38.0 2.0 40.0 68-69 29.899306693014957 35.0 28.5 37.5 2.0 40.0 70-71 29.308893833396 34.0 28.0 37.0 2.0 39.0 72-73 28.60537032988067 34.0 26.5 36.0 2.0 39.0 74-75 27.9167082141444 34.0 26.0 36.0 2.0 39.0 76-77 27.150618748491617 33.0 24.5 35.0 2.0 37.5 78-79 26.36651154767696 32.5 22.5 35.0 2.0 37.0 80-81 25.610800881537862 32.0 19.5 35.0 2.0 36.0 82-83 24.85653863991085 31.0 17.0 35.0 2.0 36.0 84-85 24.11702392453818 31.0 9.0 34.0 2.0 35.0 86-87 23.33372812894118 30.5 4.0 34.0 2.0 35.0 88-89 22.434453589512938 29.5 2.0 34.0 2.0 35.0 90-91 21.51819325591758 29.0 2.0 34.0 2.0 35.0 92-93 20.537551984784166 28.0 2.0 34.0 2.0 35.0 94-95 19.42146429703559 26.5 2.0 33.0 2.0 35.0 96-97 18.12427399092588 24.5 2.0 33.0 2.0 34.5 98-99 16.573554166688734 10.5 2.0 32.0 2.0 34.0 100-101 12.79638646644187 2.0 2.0 28.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12876167124018068 1101 2 0.2046398905646285 1101 3 0.14183289291916168 1101 4 0.04323124163848746 1101 5 0.14261091233056078 1101 6 0.08162673413208665 1101 7 0.09423089765247994 1101 8 0.1593986348194889 1101 9 0.2091081584219694 1101 10-11 0.21940687459511565 1101 12-13 0.2526628812798819 1101 14-15 0.23999231347610106 1101 16-17 0.25508371989074163 1101 18-19 0.26209974606702957 1101 20-21 0.2540250651321898 1101 22-23 0.29449227316275994 1101 24-25 0.4797630163455082 1101 26-27 0.36763480712284746 1101 28-29 0.1533555955653938 1101 30-31 0.0907040183169272 1101 32-33 0.16883028137105072 1101 34-35 0.23999258317779493 1101 36-37 0.15073174700936676 1101 38-39 0.2086239643420882 1101 40-41 0.2219384938436164 1101 42-43 0.48257932892983746 1101 44-45 0.42509932398029804 1101 46-47 0.5582470785794698 1101 48-49 0.5483212467565863 1101 50-51 0.5894888969514582 1101 52-53 0.8449543912122763 1101 54-55 0.5135873350718825 1101 56-57 0.6006729269130631 1101 58-59 0.7393782020945423 1101 60-61 0.7949944658649457 1101 62-63 0.6560217008393785 1101 64-65 0.612337452918883 1101 66-67 0.3895953944050419 1101 68-69 0.43697266781753186 1101 70-71 0.3201016615705612 1101 72-73 0.2565597321498494 1101 74-75 0.17500207225507936 1101 76-77 -0.04368856688363465 1101 78-79 -0.2771916831057908 1101 80-81 -0.12923699443936343 1101 82-83 -0.31967189848343125 1101 84-85 -0.2773791562600749 1101 86-87 0.004024373675601822 1101 88-89 -0.022366257760882746 1101 90-91 -0.3434356048290006 1101 92-93 -0.4328775448346782 1101 94-95 -0.9932170787890513 1101 96-97 -0.9002317176529537 1101 98-99 -0.9071164550639104 1101 100-101 -0.724166140532537 1102 1 0.17545539264606802 1102 2 0.2790983417593438 1102 3 0.23623709720207842 1102 4 0.24556162064534703 1102 5 0.2202337667135268 1102 6 0.31509460491134433 1102 7 0.393319272704197 1102 8 0.29339837271442804 1102 9 0.3409116620892334 1102 10-11 0.38124975292094376 1102 12-13 0.41122318826673876 1102 14-15 0.4983689996730263 1102 16-17 0.43308360798071277 1102 18-19 0.42938093620068685 1102 20-21 0.5460756249031888 1102 22-23 0.5081167296260745 1102 24-25 0.4398376426617432 1102 26-27 0.43060260206834045 1102 28-29 0.38934733189359605 1102 30-31 0.5395946365756359 1102 32-33 0.46151990628104755 1102 34-35 0.3451765941803231 1102 36-37 0.31566945288331993 1102 38-39 0.11561164098691279 1102 40-41 0.4137058918030263 1102 42-43 0.48146206931183144 1102 44-45 0.45396364338328254 1102 46-47 0.4509356727392344 1102 48-49 0.5266195370364599 1102 50-51 0.6533939383507956 1102 52-53 0.6575014191912558 1102 54-55 0.2144706143889934 1102 56-57 0.44670424404986875 1102 58-59 0.5541700568117705 1102 60-61 0.5428096435147509 1102 62-63 0.5674051590652844 1102 64-65 0.5429259965718956 1102 66-67 0.6805515183130026 1102 68-69 0.35863160140334926 1102 70-71 0.04114199777183458 1102 72-73 -0.08941158059890952 1102 74-75 -0.13598668225997557 1102 76-77 -0.35707565629532567 1102 78-79 -0.35975367168802563 1102 80-81 -0.3104973663634958 1102 82-83 -0.31623876398672834 1102 84-85 -0.2707823548252719 1102 86-87 -0.11430464768595883 1102 88-89 -0.33445202501453863 1102 90-91 -0.8397543540283294 1102 92-93 -1.0160642416777819 1102 94-95 -1.0149688535200667 1102 96-97 -0.8378709609644908 1102 98-99 -1.0336395725826861 1102 100-101 -1.1662081598016751 1103 1 0.10044041212075072 1103 2 0.04625936576147183 1103 3 0.08823089415492547 1103 4 0.10004523638932028 1103 5 -0.04587862051640457 1103 6 -0.04994889409550041 1103 7 0.09149081636410727 1103 8 -0.005259205766876107 1103 9 0.05906833619067697 1103 10-11 0.11990408562176924 1103 12-13 -0.16391662996954892 1103 14-15 0.018852093872787634 1103 16-17 0.08609857740935212 1103 18-19 0.036350209478762 1103 20-21 0.1352513631101857 1103 22-23 0.22229038720455208 1103 24-25 0.07107744758201306 1103 26-27 -0.1028226950454112 1103 28-29 0.027992093703240073 1103 30-31 0.13947243432745182 1103 32-33 -0.0364646582295407 1103 34-35 -0.14290348505002015 1103 36-37 -0.2793479966545007 1103 38-39 -0.3090023330690457 1103 40-41 -0.3006418450279824 1103 42-43 -0.2554417612639739 1103 44-45 -0.07138257320182362 1103 46-47 -0.07034152801089988 1103 48-49 -0.16436135447124656 1103 50-51 -0.08914215361853195 1103 52-53 -0.230803506627403 1103 54-55 -0.3703677069044389 1103 56-57 -0.3441516710382899 1103 58-59 -0.18867535526364776 1103 60-61 -0.2915327946489725 1103 62-63 -0.29096229657571726 1103 64-65 -0.3804382132886204 1103 66-67 -0.6935912627382983 1103 68-69 -0.4736087084161049 1103 70-71 -0.5690855397627033 1103 72-73 -0.8068830343756694 1103 74-75 -0.6560266025857722 1103 76-77 -0.5470405075442599 1103 78-79 -0.6590812125515697 1103 80-81 -0.5504708175436264 1103 82-83 -0.6510888755084139 1103 84-85 -0.5925182648089589 1103 86-87 -1.097031269743951 1103 88-89 -1.2202005519295476 1103 90-91 -1.0968541520686514 1103 92-93 -1.118288294853098 1103 94-95 -1.1590267171861868 1103 96-97 -1.5550608363487122 1103 98-99 -1.1263530728101685 1103 100-101 -0.8318788989518922 1104 1 0.2915875413635085 1104 2 0.28911572618273595 1104 3 0.2566234839655266 1104 4 0.3428168329236527 1104 5 0.258938837192936 1104 6 0.26127606143605675 1104 7 0.21099420431146854 1104 8 0.27065702664787494 1104 9 0.3615886925641405 1104 10-11 0.40624138590500536 1104 12-13 0.28629110675642977 1104 14-15 0.31618620442576173 1104 16-17 0.2720314235459327 1104 18-19 0.44185151614667717 1104 20-21 0.33354800020077846 1104 22-23 0.48212071598410944 1104 24-25 0.3575536302338378 1104 26-27 0.4312455411876286 1104 28-29 0.2551058442313874 1104 30-31 0.25405006239916617 1104 32-33 0.23120339595675432 1104 34-35 0.1714587123429041 1104 36-37 0.1448949314292065 1104 38-39 0.09228392631366944 1104 40-41 0.30063600316902495 1104 42-43 0.32594363274464655 1104 44-45 0.35726905504724016 1104 46-47 0.17903610945003834 1104 48-49 -0.032890609748768895 1104 50-51 0.09437361768068087 1104 52-53 0.14161967120984542 1104 54-55 0.08785869352537645 1104 56-57 0.13858159917855062 1104 58-59 0.1989146900654717 1104 60-61 -0.05320575992447374 1104 62-63 -0.015615286263212624 1104 64-65 0.06200482537936125 1104 66-67 -0.005468248169343326 1104 68-69 0.03502286339325167 1104 70-71 0.12580163298349234 1104 72-73 0.021350773113805843 1104 74-75 -0.08547598941685663 1104 76-77 -0.2972240539609601 1104 78-79 -0.6539132235682672 1104 80-81 -0.6708163021061644 1104 82-83 -0.5960670064255886 1104 84-85 -0.5534492852618307 1104 86-87 -0.5315433427295666 1104 88-89 -0.6652618166589015 1104 90-91 -0.7813078683782706 1104 92-93 -1.0194503425399581 1104 94-95 -1.277330696133447 1104 96-97 -1.688065807424664 1104 98-99 -1.6766112114473746 1104 100-101 -1.2767615074887892 1105 1 0.10123191436906964 1105 2 0.21599262568010147 1105 3 0.3643555665928062 1105 4 0.14792793601102971 1105 5 0.22157266577033852 1105 6 0.25498849947867086 1105 7 0.2915497010724337 1105 8 0.31958172904698756 1105 9 0.4179731825806101 1105 10-11 0.28453846511459346 1105 12-13 0.3569262625696794 1105 14-15 0.3227672896963085 1105 16-17 0.2592247181412475 1105 18-19 0.3325173502820391 1105 20-21 0.41600906400645954 1105 22-23 0.47200552286879827 1105 24-25 0.41149032880313996 1105 26-27 0.4013699726612927 1105 28-29 0.4887152285109835 1105 30-31 0.413365988108815 1105 32-33 0.3284153291268197 1105 34-35 0.3803349444871671 1105 36-37 0.3444061201768349 1105 38-39 0.33305957489747584 1105 40-41 0.43984355870188807 1105 42-43 0.4161275034674006 1105 44-45 0.6467929185237224 1105 46-47 0.4300019661210257 1105 48-49 0.539200023277985 1105 50-51 0.4993059921504397 1105 52-53 0.4800330640903283 1105 54-55 0.6255881117204609 1105 56-57 0.4883206964838607 1105 58-59 0.6134312388040399 1105 60-61 0.8112344042742805 1105 62-63 0.7359640745967759 1105 64-65 0.4395791462292884 1105 66-67 0.4682155036500042 1105 68-69 0.4488801519226193 1105 70-71 0.32981247498141286 1105 72-73 0.2641418654438539 1105 74-75 0.03024472081618157 1105 76-77 0.2575929032823723 1105 78-79 0.09127056622546448 1105 80-81 0.4046104739433254 1105 82-83 0.2696481010294711 1105 84-85 -0.025118215865823856 1105 86-87 0.07232344562113369 1105 88-89 0.06854864509715597 1105 90-91 -0.029959466477308183 1105 92-93 -0.16260349883380698 1105 94-95 -0.3961326229910469 1105 96-97 -0.5880643133505359 1105 98-99 -0.6364520552008379 1105 100-101 -0.8657133895864195 1106 1 0.1075874966763557 1106 2 0.12547556667753668 1106 3 0.15400851754024103 1106 4 0.13619880542848506 1106 5 0.12023480648169027 1106 6 0.05729597235501416 1106 7 0.21380181425407585 1106 8 0.053763747246350135 1106 9 0.17052039733009394 1106 10-11 0.1810338524891435 1106 12-13 0.36061247301022803 1106 14-15 0.3843955553518654 1106 16-17 -0.30569803624923964 1106 18-19 0.2907761268273248 1106 20-21 0.30370640917060854 1106 22-23 0.3355597763250273 1106 24-25 0.265775996292831 1106 26-27 0.3053684685591378 1106 28-29 0.23375865229517956 1106 30-31 0.1902514729770317 1106 32-33 0.3017127387134906 1106 34-35 0.37957026823082884 1106 36-37 0.20977055102704156 1106 38-39 0.28721312161677304 1106 40-41 0.3406079619814335 1106 42-43 0.17376607672686362 1106 44-45 0.46665587052212487 1106 46-47 0.7527922530605835 1106 48-49 0.7179851536858237 1106 50-51 0.7585947101558546 1106 52-53 0.6776996584181418 1106 54-55 0.73059853260974 1106 56-57 0.5264384941616456 1106 58-59 0.6229915687237977 1106 60-61 1.144101629724453 1106 62-63 0.6850296750160716 1106 64-65 0.46938616528170485 1106 66-67 0.16221260736617182 1106 68-69 -0.044204663333506033 1106 70-71 -0.30330534316962954 1106 72-73 -0.1716802656041203 1106 74-75 -0.07072154880573223 1106 76-77 -0.7368049303737578 1106 78-79 -0.7565414564916679 1106 80-81 -0.3814593153049337 1106 82-83 -0.44278449076238857 1106 84-85 -0.6886726058104493 1106 86-87 -0.39321391603081324 1106 88-89 -0.8927469983296312 1106 90-91 -0.932442258702384 1106 92-93 -1.3584264761098517 1106 94-95 -1.4909866008771964 1106 96-97 -1.4015303953008988 1106 98-99 -1.7476959012801387 1106 100-101 -1.684717147112048 1107 1 -0.11097355898786532 1107 2 -0.2174369832664631 1107 3 -0.1404284039592234 1107 4 -0.035898313004956606 1107 5 -0.05627577300797526 1107 6 -0.16886047146530103 1107 7 -0.1084644311276719 1107 8 -0.07396471344765843 1107 9 -0.14350961643410898 1107 10-11 -0.04006271426167274 1107 12-13 -0.2049068052328593 1107 14-15 -0.10245322230888831 1107 16-17 -0.057504936182930066 1107 18-19 -0.21079811906709978 1107 20-21 -0.11853698874860186 1107 22-23 0.045062648975502384 1107 24-25 -0.0925892398997803 1107 26-27 -0.024487592426872595 1107 28-29 0.09927347249679741 1107 30-31 -0.22129262541194095 1107 32-33 -0.20095094106539335 1107 34-35 -0.17647567188652857 1107 36-37 -0.14368805327162448 1107 38-39 -0.24962980486455422 1107 40-41 -0.12315651968766161 1107 42-43 -0.06249340357695132 1107 44-45 -0.2804756584189292 1107 46-47 -0.23757280481036958 1107 48-49 -0.029578248022716025 1107 50-51 -0.25055820623915537 1107 52-53 -0.5209506695265347 1107 54-55 -0.49464958526088054 1107 56-57 -0.4583512255947255 1107 58-59 -0.5590804175008515 1107 60-61 -0.6457462953358721 1107 62-63 -0.5138714177613473 1107 64-65 -0.33053136095108115 1107 66-67 -0.2775230307745993 1107 68-69 -0.43873297468312344 1107 70-71 -0.5058648162291313 1107 72-73 -0.5007394516771946 1107 74-75 -0.5479883235799043 1107 76-77 -0.7156403784905123 1107 78-79 -0.785814071300166 1107 80-81 -0.5556043465390879 1107 82-83 -0.7728495224423462 1107 84-85 -0.6819660396726164 1107 86-87 -0.6583057795478702 1107 88-89 -0.5252149143624187 1107 90-91 -0.5776183792032441 1107 92-93 -0.7726063915185186 1107 94-95 -0.8235676397123086 1107 96-97 -0.8994574060169178 1107 98-99 -1.0385106640844697 1107 100-101 -1.3946156709430664 1108 1 0.020851583077956093 1108 2 0.01007957300803497 1108 3 -0.1601510228993277 1108 4 -0.12802258290373913 1108 5 -0.08141518553895111 1108 6 -0.20405789410021669 1108 7 -0.08534202924710144 1108 8 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-0.553700579548547 2316 60-61 -0.46720658127389214 2316 62-63 -0.36307517214419605 2316 64-65 -0.46246218257214977 2316 66-67 -0.5453302473055075 2316 68-69 -0.6335965311751188 2316 70-71 -0.6982419551805812 2316 72-73 -0.5238087780571696 2316 74-75 -0.45862327496828925 2316 76-77 -0.40705349245939004 2316 78-79 -0.38231613389653063 2316 80-81 -0.4755108906070724 2316 82-83 -0.36799132089721454 2316 84-85 -0.4366899639068471 2316 86-87 -0.30596103937043395 2316 88-89 -0.41864608984052865 2316 90-91 -0.23588127749423649 2316 92-93 -0.020581468142690795 2316 94-95 0.04917769892753299 2316 96-97 -0.42346586629478367 2316 98-99 -0.7979605307489841 2316 100-101 -0.6099921721445654 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4372.0 3 1305.0 4 1365.0 5 1463.0 6 1729.0 7 2211.0 8 2765.0 9 3437.0 10 4293.0 11 5393.0 12 6962.0 13 8576.0 14 10008.0 15 12754.0 16 15298.0 17 20349.0 18 19961.0 19 15215.0 20 15654.0 21 16446.0 22 17810.0 23 19774.0 24 21911.0 25 25082.0 26 28135.0 27 32136.0 28 36642.0 29 42411.0 30 49263.0 31 58320.0 32 70615.0 33 86916.0 34 109886.0 35 142161.0 36 165559.0 37 165239.0 38 76518.0 39 3847.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.101888359209504 18.59841379038637 14.595613527331356 21.704084323072774 2 15.990704750947124 20.060933839475286 39.83506231525882 24.113299094318773 3 19.39455198316693 25.24665039631515 29.3481579926397 26.01063962787822 4 12.883568503330963 16.939512347371817 36.321833159348444 33.855085989948776 5 13.949784445018887 37.613453481135245 32.750939428557565 15.685822645288303 6 31.58472869956517 35.53529301174716 17.38785015555731 15.49212813313037 7 28.894742346007014 33.78919067198732 19.529370933543895 17.786696048461774 8 27.541514078028978 33.681577387329206 18.9545561572875 19.822352377354317 9 27.87286341376581 16.53116490109116 19.7918426245403 35.80412906060273 10-11 25.83490450471465 26.697896971801466 25.60739863625227 21.859799887231613 12-13 26.87949380343362 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29.579420746597652 22.066837746501484 38-39 22.076692297881216 25.969213021923604 29.63673364188688 22.317361038308302 40-41 22.211611360863788 25.877203441249957 29.913544821300352 21.997640376585903 42-43 22.414310626038787 25.83893316052821 29.465721033030434 22.28103518040257 44-45 22.092394440817795 26.13353011854417 29.43059063945733 22.34348480118071 46-47 22.366192001647146 26.464606016931704 28.863226228396332 22.305975753024814 48-49 22.59248550756997 26.461334219391812 28.59461083690898 22.35156943612924 50-51 22.518513359973294 26.703634091378753 28.245638204701784 22.532214343946173 52-53 22.669858682540475 26.689202994955437 28.190426676716445 22.450511645787643 54-55 22.337050296134564 26.625958335651468 28.365940494532797 22.67105087368117 56-57 22.387836060462504 26.950486372930815 28.185072959723446 22.47660460688323 58-59 22.58973707805378 26.443584131502984 28.253218635594475 22.71346015484876 60-61 22.597617853797907 26.534459759893288 28.536408616595555 22.331513769713254 62-63 22.316832792365084 26.63675697606821 28.4812215386106 22.56518869295611 64-65 22.178968153955722 26.390203918523415 28.55093951771903 22.879888409801833 66-67 22.183786688464043 26.727025717875364 28.1865416345004 22.902645959160196 68-69 22.283842199695663 26.512972117706735 28.38334935763981 22.819836324957794 70-71 22.235267267621882 26.65731056834134 28.23429759432457 22.873124569712207 72-73 22.465339070036425 26.645958924640674 27.972029030705087 22.916672974617814 74-75 22.238284963457684 26.902577035561396 27.874452773522414 22.984685227458506 76-77 22.382856972054725 26.673835323442756 27.899153495647163 23.04415420885536 78-79 22.50771861851863 26.776417350243666 27.74930381087871 22.966560220358993 80-81 22.44897418647399 27.250557914515916 27.588749840136394 22.711718058873696 82-83 22.734525251355766 27.0611399990995 27.395082874663213 22.809251874881525 84-85 22.66419274493698 26.691453808629994 27.422733771793585 23.22161967463944 86-87 22.776265127345155 26.499567141499327 27.560146142716174 23.164021588439347 88-89 22.779231938285555 26.619464805845915 27.423372680834795 23.177930575033738 90-91 22.750782565360208 26.816411264699845 27.414894833667464 23.01791133627248 92-93 22.91237451002216 26.332319494701007 27.46753104170973 23.2877749535671 94-95 22.807232728687875 26.331226992657335 27.412600490318557 23.448939788336233 96-97 22.996676063226342 26.191380173801655 27.26366302299198 23.548280739980022 98-99 22.89443823299423 26.12898049032692 27.03296728252123 23.94361399415762 100-101 23.83662199092092 25.070233150956295 27.029905667702597 24.063239190420187 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1578.0 1 1047.5 2 844.5 3 1049.5 4 773.5 5 741.0 6 1332.5 7 2576.0 8 4015.0 9 4677.5 10 3798.0 11 2635.0 12 2415.0 13 2540.0 14 2670.0 15 2709.0 16 2765.0 17 2829.5 18 2867.0 19 3007.0 20 3177.5 21 3449.0 22 3898.5 23 4529.0 24 5167.0 25 5999.5 26 7239.0 27 8559.0 28 9764.0 29 11311.5 30 13251.5 31 15470.0 32 17812.5 33 20089.5 34 22332.0 35 24124.5 36 26576.0 37 29300.0 38 30870.5 39 31707.5 40 32804.0 41 35406.0 42 38105.5 43 40263.5 44 42270.5 45 44322.5 46 45269.0 47 45874.5 48 48275.5 49 49015.0 50 48348.0 51 47296.0 52 45897.0 53 46145.5 54 46589.5 55 44211.5 56 40467.0 57 37763.5 58 34875.5 59 30935.0 60 26644.5 61 22556.5 62 18569.0 63 14942.0 64 12280.0 65 10080.0 66 7739.5 67 6209.5 68 4727.0 69 3184.0 70 2373.5 71 1797.5 72 1484.0 73 1198.5 74 920.5 75 804.5 76 572.0 77 339.5 78 198.0 79 109.5 80 70.0 81 44.0 82 21.0 83 15.0 84 12.5 85 11.0 86 8.0 87 6.5 88 3.5 89 1.5 90 2.5 91 1.0 92 1.0 93 2.0 94 1.5 95 1.5 96 1.5 97 1.5 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1441237239754543 2 0.05114311674929509 3 0.15115968530338988 4 0.13186753327517947 5 0.1988226491377921 6 0.20071403659153825 7 0.1621297325351174 8 0.07293190021645038 9 0.12301583999164763 10-11 0.10406413770511151 12-13 0.14960874759131806 14-15 0.13659600190954477 16-17 0.13425068146689959 18-19 0.13281322700205253 20-21 0.13349412648540115 22-23 0.19095447733020826 24-25 0.21436985400758524 26-27 0.1485117428681453 28-29 0.12664730390284018 30-31 0.10107574552819265 32-33 0.12611771541579125 34-35 0.09305626272430909 36-37 0.08700382287232152 38-39 0.05072701150947093 40-41 0.06109181475599967 42-43 0.06298320220974579 44-45 0.041118763244440644 46-47 0.053639748188239966 48-49 0.04342625593801091 50-51 0.05356409269009012 52-53 0.02716032383579428 54-55 0.05110528900022016 56-57 0.027765567820993042 58-59 0.0518618439817186 60-61 0.0373359883369484 62-63 0.052429260217842444 64-65 0.0391895480416196 66-67 0.03941651453606913 68-69 0.06589593888851482 70-71 0.05428281992251364 72-73 0.052731882210441824 74-75 0.04335060043986107 76-77 0.02175095571808038 78-79 0.022772304943103283 80-81 0.025874180367246917 82-83 0.022507510699578827 84-85 0.019859568264334257 86-87 0.025193280883898318 88-89 0.06495024516164176 90-91 0.10924653932837589 92-93 0.08004351704253579 94-95 0.0761850866368937 96-97 0.06865736457098415 98-99 0.05628769062348452 100-101 0.12963569607975906 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1321781.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.82796409154154 #Duplication Level Percentage of deduplicated Percentage of total 1 80.64592286033093 45.82943608436115 2 11.29277219619172 12.834905057182835 3 3.353687425031336 5.717496856918055 4 1.4744927717897622 3.351696891540247 5 0.7733740275636887 2.197463573386008 6 0.49154325525156845 1.6760041475325342 7 0.3131550407389079 1.245717438713712 8 0.2333010055069592 1.060641693077601 9 0.17384724916359404 0.8891446709593798 >10 1.0826286016560738 11.793776677083653 >50 0.10304772198139088 4.0081341922965485 >100 0.058496854223555134 5.942837858778638 >500 0.002398493938250094 0.9106456491512165 >1k 0.0010659973058889307 1.1300911082598333 >5k 1.3324966323611633E-4 0.6523542547345527 >10k+ 1.3324966323611633E-4 0.7596538460240316 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10032 0.758975957439243 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8615 0.6517721165609128 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4803 0.36337335761370454 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2326 0.17597468869653898 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1801 0.13625555216787047 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1532 0.11590422316556223 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.56554981498448E-5 0.0 14-15 0.0 0.0 0.0 1.134832472247672E-4 0.0 16-17 0.0 0.0 0.0 1.513109962996896E-4 0.0 18-19 0.0 0.0 0.0 1.513109962996896E-4 0.0 20-21 0.0 0.0 0.0 2.269664944495344E-4 0.0 22-23 0.0 0.0 0.0 4.917607379739912E-4 0.0 24-25 0.0 0.0 0.0 0.0010970047231727495 0.0 26-27 0.0 0.0 0.0 0.0017400764574464303 0.0 28-29 0.0 0.0 0.0 0.00457715763806561 0.0 30-31 0.0 0.0 0.0 0.01403409490679621 0.0 32-33 0.0 0.0 0.0 0.0312457207358859 0.0 34-35 0.0 0.0 0.0 0.05840604457168018 0.0 36-37 0.0 0.0 0.0 0.10546376442088365 0.0 38-39 0.0 0.0 0.0 0.174991167220591 0.0 40-41 0.0 0.0 0.0 0.24954966064726303 0.0 42-43 0.0 0.0 0.0 0.3235785655868862 0.0 44-45 0.0 0.0 0.0 0.4107336994555074 0.0 46-47 0.0 0.0 0.0 0.5101071962753285 0.0 48-49 0.0 0.0 0.0 0.6181810753823818 0.0 50-51 0.0 0.0 0.0 0.7365441022378141 0.0 52-53 0.0 0.0 0.0 0.8506704211968549 0.0 54-55 0.0 0.0 0.0 0.9697143475356357 0.0 56-57 0.0 0.0 0.0 1.1071803876739037 0.0 58-59 0.0 0.0 0.0 1.260571910172714 0.0 60-61 7.56554981498448E-5 0.0 0.0 1.4229664369513557 0.0 62-63 7.56554981498448E-5 0.0 0.0 1.584263959006825 0.0 64-65 7.56554981498448E-5 0.0 0.0 1.7487390119845876 0.0 66-67 7.56554981498448E-5 0.0 0.0 1.9337923604591079 0.0 68-69 7.56554981498448E-5 0.0 0.0 2.1341281195598967 0.0 70-71 7.56554981498448E-5 0.0 0.0 2.3483844903202575 0.0 72-73 7.56554981498448E-5 0.0 0.0 2.5625652055824677 0.0 74-75 7.56554981498448E-5 0.0 0.0 2.775497605125206 0.0 76-77 7.56554981498448E-5 0.0 0.0 3.0008753341135934 0.0 78-79 7.56554981498448E-5 0.0 0.0 3.252808142952577 0.0 80-81 7.56554981498448E-5 0.0 0.0 3.5244492090595947 3.78277490749224E-5 82-83 7.56554981498448E-5 0.0 0.0 3.7954472034323388 7.56554981498448E-5 84-85 7.56554981498448E-5 0.0 0.0 4.0717410826755716 7.56554981498448E-5 86-87 7.56554981498448E-5 0.0 0.0 4.347391890184531 7.56554981498448E-5 88-89 7.56554981498448E-5 0.0 0.0 4.6415404669911275 7.56554981498448E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4360 0.0 56.03264 1 GTATCAA 7770 0.0 42.351345 1 TGGTATC 1810 0.0 34.163628 2 GTGGTAT 1875 0.0 33.2721 1 TATCAAC 10235 0.0 32.020683 2 ATCAACG 10240 0.0 31.958597 3 TCAACGC 10355 0.0 31.603672 4 CAACGCA 10510 0.0 31.182846 5 AACGCAG 10810 0.0 30.310556 6 ACGCAGA 12315 0.0 26.602306 7 AACCGAT 90 8.895369E-4 26.415693 7 CGCAGAG 12475 0.0 26.180916 8 GTACATG 9100 0.0 25.119446 1 GCAGAGT 12895 0.0 24.995426 9 TACATGG 9225 0.0 24.389 2 GAGTACT 6940 0.0 24.380575 12-13 ACATGGG 9280 0.0 23.32183 3 GTACTTT 7395 0.0 22.688534 14-15 GTACACG 170 3.0800584E-6 22.410484 1 CAGAGTA 12650 0.0 21.999584 10-11 >>END_MODULE