FastQCFastQC Report
Thu 26 May 2016
SRR938252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321781
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96840.732647844083097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87410.6613047093277934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50990.3857673850660586No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24440.18490203747822068No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17740.13421285371782468No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15860.11998962006565385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46000.056.3908351
GTATCAA79800.040.126451
ATCAACG101150.031.411313
TATCAAC102150.031.389892
TCAACGC103200.030.786184
CAACGCA104950.030.2275835
GTGGTAT20550.029.8230171
AACGCAG109050.029.09116
TGGTATC20950.028.5700422
GTACTAG3050.028.0379581
ACGCAGA125000.025.2660567
CGCAGAG125800.025.1053818
GTACATG86900.024.5471061
TACATGG87600.024.077052
GCAGAGT131800.023.8543939
GAGTACT74300.023.62023712-13
ACATGGG88700.022.8092923
GTACTTT76450.022.5823514-15
ACCGTCG1503.0373154E-522.1632068
CATGGGG42900.021.9188254
Produced by FastQC (version 0.11.5)