##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938252_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1321781 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.530186165484295 34.0 31.0 34.0 31.0 34.0 2 32.74071877262572 34.0 31.0 34.0 31.0 34.0 3 32.780739018037025 34.0 31.0 34.0 31.0 34.0 4 36.2366836866319 37.0 35.0 37.0 35.0 37.0 5 36.21808302585678 37.0 37.0 37.0 35.0 37.0 6 36.25934629110269 37.0 37.0 37.0 35.0 37.0 7 36.21893415021096 37.0 37.0 37.0 35.0 37.0 8 36.21158648823065 37.0 37.0 37.0 35.0 37.0 9 37.894366767263264 39.0 38.0 39.0 35.0 39.0 10-11 37.87936882131004 39.0 38.0 39.0 35.0 39.0 12-13 37.79230447403919 39.0 38.0 39.0 35.0 39.0 14-15 39.05155089988432 40.0 38.5 41.0 35.5 41.0 16-17 38.942141701234924 40.0 38.0 41.0 35.5 41.0 18-19 39.21173250334208 40.5 39.0 41.0 36.0 41.0 20-21 39.21575094512631 41.0 39.0 41.0 36.0 41.0 22-23 39.11133765729724 40.5 39.0 41.0 35.5 41.0 24-25 39.00623514788002 40.0 38.5 41.0 35.0 41.0 26-27 38.83698434158155 40.0 38.0 41.0 35.0 41.0 28-29 38.71717742954392 40.0 38.0 41.0 35.0 41.0 30-31 38.45522026720009 40.0 38.0 41.0 34.0 41.0 32-33 38.214845348813455 40.0 37.5 41.0 34.0 41.0 34-35 38.03876814691692 40.0 37.0 41.0 34.0 41.0 36-37 38.01288526616739 40.0 37.0 41.0 34.0 41.0 38-39 37.86684783636623 40.0 37.0 41.0 33.0 41.0 40-41 37.680061976984085 40.0 37.0 41.0 33.0 41.0 42-43 37.41751697141962 40.0 36.5 41.0 33.0 41.0 44-45 37.08499970872633 40.0 36.0 41.0 32.0 41.0 46-47 36.59233602238192 39.5 35.5 41.0 30.5 41.0 48-49 36.123047237023385 39.0 35.0 41.0 30.0 41.0 50-51 35.71960861897697 39.0 35.0 40.5 28.5 41.0 52-53 35.21197384438118 38.5 35.0 40.0 27.0 41.0 54-55 34.89694661975017 38.0 34.0 40.0 26.0 41.0 56-57 34.56535500207674 38.0 34.0 40.0 26.0 41.0 58-59 34.13337307768836 37.5 33.5 40.0 24.5 41.0 60-61 33.669314735194405 37.0 33.0 40.0 23.0 41.0 62-63 33.22193805176501 36.0 33.0 39.0 22.0 41.0 64-65 32.751052178840524 36.0 32.0 39.0 20.5 40.0 66-67 32.227194974053944 35.0 31.5 38.5 18.5 40.0 68-69 31.671596883296097 35.0 31.0 38.0 15.0 40.0 70-71 31.090511590044038 35.0 30.5 37.0 10.0 39.5 72-73 30.530476304319702 34.5 30.0 37.0 7.5 39.0 74-75 29.948817920669157 34.0 29.0 36.0 6.0 39.0 76-77 28.600576419240404 32.5 27.0 35.0 2.0 37.0 78-79 29.287822264051307 34.0 29.0 35.0 2.0 37.0 80-81 28.973354133551624 34.0 29.0 35.0 2.0 37.0 82-83 28.4347660467203 34.0 27.5 35.0 2.0 36.0 84-85 27.86604286186592 33.0 26.5 35.0 2.0 36.0 86-87 27.243929591967202 33.0 26.0 35.0 2.0 35.5 88-89 26.533133325414724 32.0 25.0 35.0 2.0 35.0 90-91 26.012532333268524 32.0 24.0 34.5 2.0 35.0 92-93 25.212622212000326 31.5 19.5 34.0 2.0 35.0 94-95 24.414805100088444 31.0 15.0 34.0 2.0 35.0 96-97 23.39909183140021 31.0 2.0 34.0 2.0 35.0 98-99 21.994242994868287 30.0 2.0 34.0 2.0 35.0 100-101 17.657015420860187 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15617800238354818 1101 2 0.1631258382621752 1101 3 0.19667462824568815 1101 4 0.14542073353447194 1101 5 0.19930550047755702 1101 6 0.15531874420891967 1101 7 0.11783052106522973 1101 8 0.07962906322943297 1101 9 -0.02352744661763495 1101 10-11 0.14854857080263884 1101 12-13 0.23955971304987145 1101 14-15 0.228404383985378 1101 16-17 0.22259410102666521 1101 18-19 0.2883977872660921 1101 20-21 0.2876266233847389 1101 22-23 0.3358336050883466 1101 24-25 0.29911334533629486 1101 26-27 0.3074778666424294 1101 28-29 0.2333563435781869 1101 30-31 0.2409128255308488 1101 32-33 0.2932291348499376 1101 34-35 0.23729513973352567 1101 36-37 0.221560516315634 1101 38-39 0.3814973658601275 1101 40-41 0.3197556624325273 1101 42-43 0.4515949472790268 1101 44-45 0.5696345476925515 1101 46-47 0.6418963783761313 1101 48-49 0.5331889076595999 1101 50-51 0.4430038781632675 1101 52-53 0.21108245092755595 1101 54-55 0.18930680807837774 1101 56-57 0.553796754512689 1101 58-59 0.5493444295007066 1101 60-61 0.23824856868985478 1101 62-63 0.2679539579847088 1101 64-65 0.48154539135868646 1101 66-67 0.19979842301923156 1101 68-69 0.43315732388305506 1101 70-71 0.35310397167566876 1101 72-73 0.30865103334076593 1101 74-75 0.22660073811015025 1101 76-77 0.22563561448723846 1101 78-79 0.23515713440184882 1101 80-81 0.3080951644364731 1101 82-83 0.17858691520320846 1101 84-85 0.15026446591725318 1101 86-87 -0.2997326052949738 1101 88-89 -0.178202636383876 1101 90-91 0.2358651588568499 1101 92-93 0.013711795367136403 1101 94-95 -0.09164616511036883 1101 96-97 -8.629701721147853E-4 1101 98-99 -0.203861376510325 1101 100-101 -0.9591806957256139 1102 1 0.149665869717154 1102 2 0.1256595401132543 1102 3 0.10239190523920172 1102 4 -0.011658238130202392 1102 5 0.03903562061885424 1102 6 0.06820120776085759 1102 7 0.1290701160098422 1102 8 0.1190272817985445 1102 9 0.14434495239461143 1102 10-11 0.1366662294682257 1102 12-13 0.25666059551931397 1102 14-15 0.21334438695982527 1102 16-17 0.2782170643746582 1102 18-19 0.25546789447884066 1102 20-21 0.10441445228609325 1102 22-23 0.2374801549464749 1102 24-25 0.0858279320717159 1102 26-27 0.19613401867514568 1102 28-29 0.03972402396315999 1102 30-31 0.18991866693961867 1102 32-33 0.18580103335932563 1102 34-35 0.21127421844897043 1102 36-37 0.19431005726364958 1102 38-39 0.1002244040582525 1102 40-41 -0.03373276445120865 1102 42-43 0.09286736425577402 1102 44-45 0.027477967898498434 1102 46-47 0.11639893095543385 1102 48-49 0.07085620210224164 1102 50-51 -0.0957099958820038 1102 52-53 -0.06601781309174726 1102 54-55 0.08301842183565356 1102 56-57 0.17992303319515202 1102 58-59 0.01735840941874045 1102 60-61 -0.10869348989503891 1102 62-63 0.07644829695723132 1102 64-65 0.014688428567296796 1102 66-67 -0.07394306776954096 1102 68-69 -0.20616218954393517 1102 70-71 -0.13752025066009566 1102 72-73 -0.23091909964073665 1102 74-75 -0.21828946490346723 1102 76-77 -0.2997344616557527 1102 78-79 -0.39341754418814645 1102 80-81 -0.15776233876253443 1102 82-83 -0.03601528147276767 1102 84-85 -0.2849171743597516 1102 86-87 -0.6649594366068072 1102 88-89 -0.4573180170914739 1102 90-91 -0.6318825930032865 1102 92-93 -0.5671652847236288 1102 94-95 -0.5995494424543324 1102 96-97 -0.8679473606379702 1102 98-99 -0.8590227846329341 1102 100-101 -1.5449221806243862 1103 1 -2.0094085456889843E-5 1103 2 0.024478575367425037 1103 3 0.014334158088274762 1103 4 -0.002055443984815497 1103 5 0.023107715784028926 1103 6 7.652633774455353E-4 1103 7 0.01090428714292102 1103 8 0.04976593266353291 1103 9 0.019626530787142826 1103 10-11 0.07188794124292741 1103 12-13 0.032575517232196205 1103 14-15 -0.006059277165697097 1103 16-17 0.07093375489920106 1103 18-19 -0.013683597470517839 1103 20-21 0.011613565660923086 1103 22-23 -0.013172468864802056 1103 24-25 -0.057039118540267 1103 26-27 0.03352110990099533 1103 28-29 0.16198314266440406 1103 30-31 0.3469242818624423 1103 32-33 0.11275294032471805 1103 34-35 0.17385272749832126 1103 36-37 0.19487182696304473 1103 38-39 0.20801344497173346 1103 40-41 0.2878907915926021 1103 42-43 0.18439698685750727 1103 44-45 0.2145236973391107 1103 46-47 -0.06928190617851016 1103 48-49 -0.10820268800728883 1103 50-51 -0.11575269037916058 1103 52-53 0.032336964586157535 1103 54-55 0.15333464962841248 1103 56-57 0.05946504524615648 1103 58-59 0.14856839199695315 1103 60-61 0.15759367013026093 1103 62-63 0.3071483868403888 1103 64-65 0.18938909547809146 1103 66-67 -0.019897354973956283 1103 68-69 0.10544799499394131 1103 70-71 -0.18471722792298806 1103 72-73 -0.37978252163658865 1103 74-75 -0.4974099416170752 1103 76-77 -0.33849518382804433 1103 78-79 -0.2823993678174297 1103 80-81 -0.4172770529673997 1103 82-83 -0.36149146798585363 1103 84-85 -0.32353363962157644 1103 86-87 -0.9096947529178259 1103 88-89 -0.9887958459140052 1103 90-91 -1.180235706178383 1103 92-93 -1.2261377871371586 1103 94-95 -1.143345979178811 1103 96-97 -1.093075527354575 1103 98-99 -1.1633022213651785 1103 100-101 -1.4186495295152746 1104 1 0.12530396477458794 1104 2 0.13555904107325745 1104 3 0.11107192209517791 1104 4 0.08464171159995004 1104 5 0.012400732804614734 1104 6 -0.03821504010335275 1104 7 -0.012288416381686318 1104 8 0.07615293066571382 1104 9 0.16962079292402876 1104 10-11 0.07433500945568028 1104 12-13 0.17378106835394647 1104 14-15 0.09355785087732471 1104 16-17 0.09018819783093335 1104 18-19 0.051633497710199094 1104 20-21 0.04317670302015131 1104 22-23 0.10204154038881086 1104 24-25 6.290138896147823E-4 1104 26-27 0.03002199144542317 1104 28-29 -0.130486824838421 1104 30-31 -0.1886837229782259 1104 32-33 -0.06886281584740317 1104 34-35 -0.07165185235968607 1104 36-37 -0.10039013256937324 1104 38-39 -0.03738387596781223 1104 40-41 0.01078721273088945 1104 42-43 -0.07739497387464667 1104 44-45 8.801617651243987E-5 1104 46-47 -0.1431347247733612 1104 48-49 -0.056205911643111506 1104 50-51 0.09470397832183863 1104 52-53 0.011005480409622237 1104 54-55 -0.23114879924462173 1104 56-57 -0.11834448638772699 1104 58-59 -0.14636038025683007 1104 60-61 -0.44115365002685536 1104 62-63 -0.16849829641392233 1104 64-65 -0.16636363391906883 1104 66-67 -0.30807859581293684 1104 68-69 -0.3684975422526122 1104 70-71 -0.5354206377785502 1104 72-73 -0.5620229224524067 1104 74-75 -0.2764500492802533 1104 76-77 -0.5046467468741156 1104 78-79 -0.5679904198807115 1104 80-81 -0.5710912322704083 1104 82-83 -0.5454398611233735 1104 84-85 -0.6933715189073695 1104 86-87 -0.8846877761979002 1104 88-89 -0.8926413226521852 1104 90-91 -0.9579316542037724 1104 92-93 -1.0226751171529607 1104 94-95 -1.0216064546911774 1104 96-97 -1.020976017159434 1104 98-99 -1.0423454587718766 1104 100-101 -1.6060393364622598 1105 1 0.09363208337418882 1105 2 0.13786987572532183 1105 3 0.12230984966495839 1105 4 0.03403119555920142 1105 5 0.013878621130047009 1105 6 -0.027849900836969255 1105 7 0.02875068727343688 1105 8 0.0412837306303544 1105 9 0.01563715552585876 1105 10-11 0.04898026022980417 1105 12-13 0.07965906724086835 1105 14-15 0.1325058732834563 1105 16-17 0.06020960044282475 1105 18-19 0.1670376509872682 1105 20-21 0.1797861258966691 1105 22-23 0.1995625601442086 1105 24-25 0.17386286263701578 1105 26-27 0.22480910455803382 1105 28-29 0.1682781983882009 1105 30-31 0.16031456257171328 1105 32-33 0.23316707061541564 1105 34-35 0.24777279214404757 1105 36-37 0.2651319903107563 1105 38-39 0.2883834244767982 1105 40-41 0.1635369939112863 1105 42-43 0.20268820870245463 1105 44-45 0.15572820826464806 1105 46-47 -0.10717204923521706 1105 48-49 0.05701097936293564 1105 50-51 0.05538959648878716 1105 52-53 -0.09282426735331484 1105 54-55 0.1743833837346358 1105 56-57 0.21774064097586177 1105 58-59 0.1718979526303528 1105 60-61 -0.02178291669792287 1105 62-63 0.01621969086901487 1105 64-65 0.06808149807615393 1105 66-67 0.15351747377605562 1105 68-69 0.1988898699238426 1105 70-71 0.06226971037807161 1105 72-73 -0.2621494829817088 1105 74-75 -0.24021377141732714 1105 76-77 0.17525222881356228 1105 78-79 0.16518941808694976 1105 80-81 -0.016308514225851667 1105 82-83 -0.21014565925154827 1105 84-85 0.03152585687593046 1105 86-87 0.060014672714100215 1105 88-89 -0.4623034626892668 1105 90-91 -0.345529033597753 1105 92-93 -0.18021153832249936 1105 94-95 -0.5641109960539978 1105 96-97 -0.465008934102606 1105 98-99 -0.4994039007264739 1105 100-101 -1.2163085437189771 1106 1 0.15361327527278945 1106 2 0.14760013370204206 1106 3 0.20457140784381522 1106 4 0.13493570418049217 1106 5 0.09284807912096227 1106 6 0.15291260525383166 1106 7 0.14364320948351406 1106 8 0.08840908021126381 1106 9 0.07962086973007132 1106 10-11 0.10996498308328029 1106 12-13 0.1389840911604523 1106 14-15 0.14887356211174563 1106 16-17 0.28645277090454613 1106 18-19 0.1714394018604466 1106 20-21 0.23323430056905892 1106 22-23 0.35485735461315215 1106 24-25 0.24469522349050266 1106 26-27 0.3299321283766261 1106 28-29 0.0892964564413532 1106 30-31 0.17173386448814654 1106 32-33 0.22421786750293649 1106 34-35 0.24233543554217363 1106 36-37 0.1824882646142214 1106 38-39 0.2313974704979529 1106 40-41 0.24544572957830013 1106 42-43 0.25538535595842404 1106 44-45 0.03075806510196344 1106 46-47 0.0809290968578722 1106 48-49 0.235770859416931 1106 50-51 0.02326807540747211 1106 52-53 0.11306814157138234 1106 54-55 -0.05436503129382686 1106 56-57 -0.1646902993063719 1106 58-59 -0.11822178864223787 1106 60-61 0.014870701677786258 1106 62-63 0.07603573180461609 1106 64-65 -0.07341261313186465 1106 66-67 -0.2389706141989052 1106 68-69 -0.6002220683069659 1106 70-71 -0.8357130593264159 1106 72-73 -0.9890344901405257 1106 74-75 -0.6333010434646376 1106 76-77 -0.7921645780949333 1106 78-79 -0.5391347391313062 1106 80-81 -0.6212906137723024 1106 82-83 -0.575025897505693 1106 84-85 -0.8009537415206047 1106 86-87 -0.6804894881347465 1106 88-89 -0.9635751933485182 1106 90-91 -1.155838480096346 1106 92-93 -0.9647577758150447 1106 94-95 -1.0127974999445968 1106 96-97 -1.2110378041288001 1106 98-99 -0.7898525790410602 1106 100-101 -1.6181471106999759 1107 1 0.06716070604775837 1107 2 -0.0022012048249067107 1107 3 -0.04513464004669032 1107 4 -0.06788846509134316 1107 5 -0.05426728941974801 1107 6 -0.03186764116441054 1107 7 -0.03510753584529169 1107 8 -0.049121499166950855 1107 9 0.04415714982881269 1107 10-11 0.08140565491013518 1107 12-13 -0.014222076539923023 1107 14-15 -1.8941329658161976E-4 1107 16-17 -0.047223274366857027 1107 18-19 -0.006882256976780354 1107 20-21 0.005199906779111529 1107 22-23 0.08081040029136233 1107 24-25 0.034922082075858896 1107 26-27 0.1682027426390178 1107 28-29 0.13333633362231723 1107 30-31 0.11201004015727278 1107 32-33 0.06773503932142688 1107 34-35 0.07113271167883539 1107 36-37 0.029167859601152202 1107 38-39 0.016436283321510814 1107 40-41 -0.0607520625766611 1107 42-43 0.09841492949103525 1107 44-45 0.14311742782494008 1107 46-47 0.29676238839706315 1107 48-49 0.08178732073506723 1107 50-51 0.11760301920828908 1107 52-53 0.11716118089688621 1107 54-55 0.13123159396878492 1107 56-57 0.02761010581815526 1107 58-59 0.16274348878726386 1107 60-61 0.04740883186374134 1107 62-63 0.022678547949411154 1107 64-65 -0.17999602587406116 1107 66-67 -0.1085255344835474 1107 68-69 0.05061576486197694 1107 70-71 -0.04585956171425565 1107 72-73 -0.27265230224521986 1107 74-75 -0.31829583312866916 1107 76-77 -0.39751645688078696 1107 78-79 -0.33815787079398874 1107 80-81 -0.18129863824695036 1107 82-83 -0.05045373017310695 1107 84-85 -0.052635259037920434 1107 86-87 -0.3016200474517774 1107 88-89 -0.22264591460390193 1107 90-91 -0.46289723151037876 1107 92-93 -0.38037262332216315 1107 94-95 -0.7605949488303665 1107 96-97 -0.7039940986047277 1107 98-99 -0.5824979251243931 1107 100-101 -1.014974099683041 1108 1 -0.08419928366564022 1108 2 -0.06726089619625952 1108 3 -0.08062086309347904 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quality scores pass #Quality Count 2 1.0 3 3.0 4 10.0 5 52.0 6 62.0 7 150.0 8 318.0 9 594.0 10 1077.0 11 1741.0 12 2833.0 13 4420.0 14 5683.0 15 8082.0 16 11368.0 17 16616.0 18 17146.0 19 11510.0 20 10292.0 21 10777.0 22 11103.0 23 12072.0 24 13628.0 25 15623.0 26 18273.0 27 21156.0 28 25433.0 29 31030.0 30 37507.0 31 45858.0 32 57125.0 33 74176.0 34 101567.0 35 148173.0 36 203097.0 37 253884.0 38 142259.0 39 7082.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.27351656042522 18.98923714226266 14.980006431233095 21.75723986607903 2 16.238859004693616 20.272355198273587 39.530245168105225 23.958540628927583 3 19.498429992812017 25.155373964362727 29.61918813604207 25.72700790678319 4 13.075312778743225 17.233792890047596 36.10378723858188 33.58710709262729 5 13.815588886156874 37.68538518280343 32.91361994287984 15.585405988159861 6 31.626189040513434 35.7543243511109 17.195267821138348 15.42421878723731 7 28.84275080365053 33.75506229851995 19.49301737579826 17.90916952203126 8 27.521957116950542 33.972496200202606 18.875517199899228 19.630029482947627 9 27.490106152229455 16.619243278576405 19.80426409518672 36.08638647400742 10-11 25.864534291232815 26.760484528072354 25.508764311183167 21.866216869511668 12-13 26.70960410601558 24.20965706538515 26.908315226272688 22.172423602326578 14-15 23.530335206815653 25.286866735109676 25.886966146434244 25.29583191164043 16-17 22.288758967801975 28.829026833895405 26.321011846104668 22.561202352197952 18-19 21.95813829976373 26.335830216957273 29.858312383064973 21.84771910021403 20-21 23.13893148713743 25.23205432670011 30.097572895963854 21.531441290198604 22-23 22.755509422514017 25.276010171125172 30.09439536504156 21.87408504131925 24-25 22.293046398325842 25.744218130728484 30.153483345243142 21.809252125702532 26-27 21.926358363569637 26.160941632268536 30.10340248271896 21.809297521442865 28-29 21.66573478107246 26.060136506737948 30.367415125902912 21.90671358628668 30-31 21.94331692812856 25.759891354923642 30.308609517036324 21.98818219991148 32-33 21.780045257118992 25.956985309971927 30.0994642834176 22.16350514949148 34-35 22.4049894961361 26.06827114306932 29.843306486972978 21.6834328738216 36-37 22.46322454372787 25.779607140871168 29.751511513147317 22.005656802253643 38-39 21.937611311099108 26.012259541545802 29.766649568071323 22.283479579283767 40-41 22.127869482520953 25.863852123768872 29.958449151657568 22.049829242052606 42-43 22.32317882798876 25.83535402063182 29.543974064588856 22.297493086790567 44-45 22.06024373941059 26.140157159225758 29.444071840975113 22.355527260388545 46-47 22.26279884912682 26.445305909686866 28.91795829711321 22.3739369440731 48-49 22.460387442156225 26.39888967192892 28.71407803845067 22.426644847464182 50-51 22.41149593824406 26.662172519272247 28.36382014633973 22.56251139614396 52-53 22.68117367552926 26.69899974274776 28.188074088646097 22.431752493076885 54-55 22.366172609065245 26.59883352519853 28.366023555724198 22.668970310012035 56-57 22.37254944245879 26.918724554248897 28.204665465814493 22.504060537477823 58-59 22.571020045396224 26.43635844932426 28.246645511021583 22.745975994257932 60-61 22.51858930127833 26.629268654367618 28.473067075828006 22.37907496852605 62-63 22.317508554582094 26.66203309234711 28.40730404672217 22.613154306348626 64-65 22.081855529213414 26.43380736572635 28.48280626773081 23.001530837329426 66-67 22.125954849715328 26.778807793684738 28.151793817912036 22.943443538687895 68-69 22.152551819858097 26.54443591380378 28.328138639854306 22.97487362648382 70-71 22.171268285153044 26.676611953715444 28.192164738143592 22.959955022987916 72-73 22.286738714636776 26.72685123536746 27.88538652898665 23.101023521009115 74-75 22.126960693994587 27.02887066220277 27.678175861751942 23.165992782050697 76-77 22.092845516520153 26.83723960599528 27.973631619053098 23.09628325843147 78-79 22.276392678928993 26.889483187356884 27.845897600053277 22.988226533660853 80-81 22.049404994977785 27.401631700308897 27.73761895844328 22.811344346270033 82-83 22.34168554921344 27.21599363064073 27.549181655397685 22.89313916474814 84-85 22.272539984207228 26.956531280398917 27.482112054532365 23.288816680861498 86-87 22.288710077173253 26.798473562626878 27.684480778581445 23.228335581618424 88-89 22.180863340383432 27.055720982674924 27.416789318158603 23.346626358783045 90-91 22.160415885468794 27.120377563101556 27.59592605387456 23.123280497555097 92-93 22.26019350471616 26.77907461142602 27.504442980582557 23.45628890327526 94-95 22.12112762699764 26.78910508898565 27.545845362724204 23.543921921292505 96-97 22.16622054915017 26.713933053997557 27.403444720837626 23.71640167601464 98-99 22.04547612679261 26.661753573310705 27.194841857285486 24.0979284426112 100-101 22.45490836398964 26.26357969858706 27.10026618403471 24.181245753388588 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1668.0 1 1076.0 2 835.5 3 1069.5 4 765.5 5 680.5 6 1347.0 7 2766.5 8 4162.0 9 4746.0 10 3852.5 11 2590.0 12 2318.0 13 2462.0 14 2598.5 15 2697.0 16 2665.0 17 2690.5 18 2834.0 19 2964.5 20 3092.0 21 3394.0 22 3879.5 23 4370.5 24 4933.5 25 5729.5 26 7020.5 27 8499.0 28 9814.5 29 11386.0 30 13294.0 31 15464.5 32 17861.0 33 20075.0 34 22378.5 35 24313.0 36 26809.5 37 29683.0 38 31046.0 39 31950.5 40 33138.0 41 35493.5 42 38284.0 43 40808.0 44 42912.0 45 44975.5 46 45738.0 47 46464.0 48 49474.5 49 50499.0 50 49594.0 51 47810.0 52 45921.0 53 46287.5 54 46964.5 55 44641.5 56 40676.0 57 37618.5 58 34599.0 59 30391.5 60 25974.5 61 21703.0 62 17797.0 63 14148.0 64 11443.5 65 9440.0 66 7028.5 67 5738.0 68 4342.0 69 2671.0 70 1873.5 71 1445.5 72 1231.5 73 1037.0 74 847.0 75 716.5 76 484.0 77 256.5 78 146.5 79 96.0 80 55.5 81 31.0 82 16.5 83 3.5 84 3.5 85 5.5 86 4.5 87 2.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008019482803883548 2 0.014979788633669269 3 0.009910870257629668 4 0.0 5 4.539329888990688E-4 6 0.0016644209592965855 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.835214759479822E-4 14-15 0.0 16-17 0.0029127366787690245 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003139703173218559 26-27 0.018611252544861817 28-29 0.006657683837186342 30-31 0.0034801529148928605 32-33 0.0 34-35 0.009419109519655676 36-37 7.187272324235256E-4 38-39 0.0026479424352445677 40-41 0.002042698450045809 42-43 0.0027235979333944125 44-45 3.782774907492239E-5 46-47 1.5131099629968957E-4 48-49 7.187272324235256E-4 50-51 0.004690640885290377 52-53 0.00915431527613122 54-55 0.00805731055295847 56-57 0.016606381843890933 58-59 0.023112754684777585 60-61 0.004009741401941774 62-63 0.020200018006008562 64-65 0.021334850478256232 66-67 0.013050573430848226 68-69 0.030300027009012836 70-71 0.016417243098516318 72-73 0.00522022937233929 74-75 0.01622810435314171 76-77 0.020729606493057474 78-79 0.025344591880198008 80-81 0.011613118966001175 82-83 0.03442325165817938 84-85 0.021977922212529914 86-87 0.03052699350346237 88-89 0.02163747247085561 90-91 0.027198151584869202 92-93 0.023793654168126187 94-95 0.014488027895695279 96-97 0.014639338891994966 98-99 0.010780908486352882 100-101 0.010516114242828426 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1321781.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.36678769303612 #Duplication Level Percentage of deduplicated Percentage of total 1 78.65387146150186 42.76158330982825 2 12.478145773995646 13.567934041951544 3 3.708539517268677 6.048641417597424 4 1.5799983978049528 3.4359774979519644 5 0.8521497800544657 2.316432308744428 6 0.5368941763571452 1.7513527019781843 7 0.3556363913910275 1.353436573067144 8 0.2599417880396669 1.1305760002320615 9 0.19943356488199954 0.9758306052724514 >10 1.1900371073921538 12.360850484554895 >50 0.11700456974880521 4.391537193547253 >100 0.06390212104646101 6.24327166199986 >500 0.002778344073206211 0.9348462366044568 >1k 0.0012502548329427947 0.9510840731588242 >5k 4.1675161098093163E-4 1.7766458935113298 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9684 0.732647844083097 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8741 0.6613047093277934 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5099 0.3857673850660586 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2444 0.18490203747822068 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1774 0.13421285371782468 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1586 0.11998962006565385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.56554981498448E-5 0.0 14-15 0.0 0.0 0.0 7.56554981498448E-5 0.0 16-17 0.0 0.0 0.0 7.56554981498448E-5 0.0 18-19 0.0 0.0 0.0 7.56554981498448E-5 0.0 20-21 0.0 0.0 0.0 1.513109962996896E-4 0.0 22-23 0.0 0.0 0.0 4.539329888990688E-4 0.0 24-25 0.0 0.0 0.0 0.0010970047231727495 0.0 26-27 0.0 0.0 0.0 0.001815731955596275 0.0 28-29 0.0 0.0 0.0 0.004728468634365299 0.0 30-31 0.0 0.0 0.0 0.014336716899395589 0.0 32-33 0.0 0.0 0.0 0.031661825975710045 0.0 34-35 0.0 0.0 0.0 0.05931391054947832 0.0 36-37 0.0 0.0 0.0 0.10697687438388054 0.0 38-39 0.0 0.0 0.0 0.17676907142711235 0.0 40-41 0.0 0.0 0.0 0.2513275648537844 0.0 42-43 0.0 0.0 0.0 0.3253942975424825 0.0 44-45 0.0 0.0 0.0 0.41232246491665414 0.0 46-47 0.0 0.0 0.0 0.5111285455003514 0.0 48-49 0.0 0.0 0.0 0.6199589795889031 0.0 50-51 0.0 0.0 0.0 0.7390029059276839 0.0 52-53 0.0 0.0 0.0 0.8536966411228486 0.0 54-55 0.0 0.0 0.0 0.973951055432027 0.0 56-57 0.0 0.0 0.0 1.1132706552749663 0.0 58-59 0.0 0.0 0.0 1.2676456992497245 0.0 60-61 0.0 0.0 0.0 1.431666819238588 0.0 62-63 0.0 0.0 0.0 1.5946665900024284 0.0 64-65 0.0 0.0 0.0 1.760654752943188 0.0 66-67 0.0 0.0 0.0 1.9471077281334805 0.0 68-69 0.0 0.0 0.0 2.1503183961639634 0.0 70-71 0.0 0.0 0.0 2.3672227093595684 0.0 72-73 0.0 0.0 0.0 2.5846566110422224 0.0 74-75 0.0 0.0 0.0 2.800615230510955 0.0 76-77 0.0 0.0 0.0 3.030456633890183 0.0 78-79 0.0 0.0 0.0 3.2862857008838833 0.0 80-81 0.0 0.0 0.0 3.5638657235956637 0.0 82-83 0.0 0.0 0.0 3.8408405023222456 0.0 84-85 0.0 0.0 0.0 4.1254943141110365 0.0 86-87 0.0 0.0 0.0 4.41147209711745 0.0 88-89 0.0 0.0 0.0 4.717309448388198 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4600 0.0 56.390835 1 GTATCAA 7980 0.0 40.12645 1 ATCAACG 10115 0.0 31.41131 3 TATCAAC 10215 0.0 31.38989 2 TCAACGC 10320 0.0 30.78618 4 CAACGCA 10495 0.0 30.227583 5 GTGGTAT 2055 0.0 29.823017 1 AACGCAG 10905 0.0 29.0911 6 TGGTATC 2095 0.0 28.570042 2 GTACTAG 305 0.0 28.037958 1 ACGCAGA 12500 0.0 25.266056 7 CGCAGAG 12580 0.0 25.105381 8 GTACATG 8690 0.0 24.547106 1 TACATGG 8760 0.0 24.07705 2 GCAGAGT 13180 0.0 23.854393 9 GAGTACT 7430 0.0 23.620237 12-13 ACATGGG 8870 0.0 22.809292 3 GTACTTT 7645 0.0 22.58235 14-15 ACCGTCG 150 3.0373154E-5 22.163206 8 CATGGGG 4290 0.0 21.918825 4 >>END_MODULE