SRR938251_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR938251_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	435616
Sequences flagged as poor quality	0
Sequence length	101
%GC	35

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8834	2.027932858297216	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8516	1.9549327848380225	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7471	1.7150426063321824	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5363	1.2311301696907369	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2625	0.6025949460074929	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	2576	0.591346507015353	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2316	0.5316609123631822	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	2172	0.4986042753250569	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	1717	0.3941544846837582	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1596	0.3663777271725557	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1415	0.32482737089546754	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	1287	0.29544369352824507	TruSeq Adapter, Index 11 (95% over 21bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	1243	0.28534305443326236	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1177	0.27019209579078823	No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	959	0.2201480202747374	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	936	0.21486814074781457	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	800	0.18364798354514067	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	794	0.18227062366855212	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	728	0.167119665026078	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	712	0.1634467053551752	No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT	709	0.16275802541688092	RNA PCR Primer, Index 11 (95% over 23bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	697	0.16000330566370383	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	695	0.15954418570484097	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	681	0.156330345992801	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	661	0.1517391464041725	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	660	0.15150958642474105	No Hit
GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	619	0.14209762726805258	No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	582	0.13360390802908984	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	544	0.12488062881069566	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	494	0.11340262983912436	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT	472	0.108352310291633	No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA	449	0.1030724307647102	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	446	0.10238375082641592	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	446	0.10238375082641592	No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	441	0.10123595092925879	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAAGCG	30	1.4933576E-6	47.493114	94-95
GACTGGT	40	5.3883245E-4	47.49311	7
GCAGATC	80	2.2608583E-7	41.556473	8
GTAGATA	105	1.02591E-9	40.70838	6
TGTAGAT	110	1.5952537E-9	38.9116	5
TCCTAAG	80	1.0653326E-5	35.677166	2
GGACTGG	80	1.0771433E-5	35.619835	6
GGACTGA	55	0.002579708	34.540447	6
ATGGGGG	555	0.0	32.56266	5
TGGGGGA	460	0.0	32.006226	6
CCCTATA	150	9.367795E-10	31.71304	2
TGGACTG	90	2.3893153E-5	31.705746	5
ACAATCA	90	2.4119388E-5	31.662075	8
AGCAGAT	125	2.1514461E-7	30.395594	7
TACAATC	95	3.489372E-5	29.995651	7
ATGGGCG	65	0.0058050393	29.266846	5
CAATCAA	100	4.9510352E-5	28.495869	9
GGATATG	135	4.1613384E-7	28.189367	1
CATGGGG	1590	0.0	28.116417	4
CATATGC	85	6.350463E-4	27.978874	3

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content