##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938251_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 435616 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42923584074047 33.0 31.0 34.0 31.0 34.0 2 32.6261707558951 34.0 31.0 34.0 31.0 34.0 3 32.654305626974214 34.0 31.0 34.0 31.0 34.0 4 36.15431710497319 37.0 35.0 37.0 35.0 37.0 5 36.10410545067215 37.0 35.0 37.0 35.0 37.0 6 36.19376698743848 37.0 37.0 37.0 35.0 37.0 7 36.162521578638064 37.0 37.0 37.0 35.0 37.0 8 36.19017437376038 37.0 37.0 37.0 35.0 37.0 9 37.827198725482994 39.0 38.0 39.0 35.0 39.0 10-11 37.83416357525894 39.0 38.0 39.0 35.0 39.0 12-13 37.77546279291853 39.0 38.0 39.0 35.0 39.0 14-15 38.89959390839638 40.0 38.0 41.0 35.5 41.0 16-17 38.83904402042165 40.0 38.0 41.0 35.5 41.0 18-19 39.10045544699919 40.0 38.5 41.0 35.5 41.0 20-21 39.105193564974655 40.0 39.0 41.0 35.0 41.0 22-23 38.963984334827 40.0 38.5 41.0 35.0 41.0 24-25 38.81741947035921 40.0 38.0 41.0 35.0 41.0 26-27 38.58035747079997 40.0 38.0 41.0 35.0 41.0 28-29 38.411924722691545 40.0 37.5 41.0 34.5 41.0 30-31 38.037938230000734 40.0 37.0 41.0 34.0 41.0 32-33 37.634240936972006 40.0 35.0 41.0 33.5 41.0 34-35 37.34340106883126 40.0 35.0 41.0 33.0 41.0 36-37 37.16620945970763 40.0 35.0 41.0 33.0 41.0 38-39 36.89075355358848 39.0 35.0 41.0 33.0 41.0 40-41 36.658582329391024 39.0 35.0 41.0 33.0 41.0 42-43 36.3050714390656 39.0 35.0 41.0 31.0 41.0 44-45 35.89248902703298 39.0 35.0 41.0 30.5 41.0 46-47 35.34928469110409 39.0 35.0 41.0 27.5 41.0 48-49 34.82429823514288 39.0 35.0 40.5 24.0 41.0 50-51 34.44063808491883 38.0 35.0 40.0 23.0 41.0 52-53 34.02182885844414 38.0 34.5 40.0 20.0 41.0 54-55 33.69469785315508 38.0 34.0 40.0 16.0 41.0 56-57 33.32710919709102 38.0 33.5 40.0 8.5 41.0 58-59 32.874403144053474 37.5 33.0 40.0 2.0 41.0 60-61 32.411389847939475 37.0 33.0 40.0 2.0 41.0 62-63 31.901115891060016 36.0 32.0 39.5 2.0 41.0 64-65 31.387623503268934 36.0 31.0 39.0 2.0 40.0 66-67 30.844263066554028 35.0 31.0 39.0 2.0 40.0 68-69 30.284044892749577 35.0 29.5 38.0 2.0 40.0 70-71 29.744353972305884 35.0 29.0 38.0 2.0 40.0 72-73 29.13818936861823 35.0 28.5 37.0 2.0 39.0 74-75 28.541960120840372 34.0 27.5 36.5 2.0 39.0 76-77 27.16552881436862 32.5 26.0 35.0 2.0 37.0 78-79 27.81660797583193 34.0 26.0 35.0 2.0 37.5 80-81 27.527311898552853 34.0 26.0 35.0 2.0 37.0 82-83 27.02884421141556 33.5 25.5 35.0 2.0 37.0 84-85 26.439048382061266 33.0 24.5 35.0 2.0 36.0 86-87 25.878265490707413 33.0 22.0 35.0 2.0 36.0 88-89 25.17863668919415 32.0 19.0 35.0 2.0 35.0 90-91 24.63946686990377 32.0 11.5 35.0 2.0 35.0 92-93 23.86715823110262 31.0 2.0 34.0 2.0 35.0 94-95 23.11166026959524 31.0 2.0 34.0 2.0 35.0 96-97 22.226312853522366 30.5 2.0 34.0 2.0 35.0 98-99 21.01883769191214 29.5 2.0 34.0 2.0 35.0 100-101 17.282886762653348 22.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19397899553147369 1101 2 0.2386963973286811 1101 3 0.33348444243546993 1101 4 0.17205412052256008 1101 5 0.006314830773412439 1101 6 0.16939003474363545 1101 7 0.19190185719072161 1101 8 0.18174435510280063 1101 9 0.031042739928608398 1101 10-11 0.025810735028670706 1101 12-13 0.37042350629904774 1101 14-15 0.5078587190970367 1101 16-17 0.4938054699581258 1101 18-19 0.3028069246186931 1101 20-21 0.2364815174763848 1101 22-23 0.5036653737389969 1101 24-25 0.438922016504236 1101 26-27 0.308714653412423 1101 28-29 0.4515190888231544 1101 30-31 0.7701299167311362 1101 32-33 0.6347104742690135 1101 34-35 0.7017822484930534 1101 36-37 0.5896823702324383 1101 38-39 0.7381178854443604 1101 40-41 0.5121069165160321 1101 42-43 0.6997586460271421 1101 44-45 0.6340089485044089 1101 46-47 0.9150105275953848 1101 48-49 0.8779501915537011 1101 50-51 0.8474560598919751 1101 52-53 0.8790245489664841 1101 54-55 0.7697894013058715 1101 56-57 0.8363515296708997 1101 58-59 0.4101099423579342 1101 60-61 0.3486435442289846 1101 62-63 0.08603109921988761 1101 64-65 0.47455317003484154 1101 66-67 0.8434569258025313 1101 68-69 1.386207621517368 1101 70-71 1.1001186800100697 1101 72-73 1.2708300868268019 1101 74-75 1.7570592629538773 1101 76-77 1.4791029098580992 1101 78-79 1.6773537800880405 1101 80-81 1.6254870443180174 1101 82-83 2.0144392815253873 1101 84-85 1.8805941195664282 1101 86-87 1.888175899209731 1101 88-89 1.852705730281901 1101 90-91 2.0321829796829647 1101 92-93 2.2472503925910843 1101 94-95 2.8684422634674256 1101 96-97 3.2055877920794735 1101 98-99 3.762487059748345 1101 100-101 3.372637027830084 1102 1 0.2739036229027505 1102 2 0.058776004374756496 1102 3 0.042241217503217854 1102 4 0.12246909342228918 1102 5 -0.1616645052699539 1102 6 0.04115070547533861 1102 7 0.14667827995494775 1102 8 0.013489781932065625 1102 9 0.35446753667658015 1102 10-11 0.25902127025902644 1102 12-13 0.414186209551076 1102 14-15 0.44906383427319696 1102 16-17 0.396202150174922 1102 18-19 0.4561986929113786 1102 20-21 0.07553131422434944 1102 22-23 0.20487429989076134 1102 24-25 0.2844398857454351 1102 26-27 0.13977198742326635 1102 28-29 0.24999258137057012 1102 30-31 0.32592895128398425 1102 32-33 0.03924553524461771 1102 34-35 0.08454181827625007 1102 36-37 -0.2322709664884286 1102 38-39 0.0485853651191519 1102 40-41 0.2700993792531605 1102 42-43 0.3718136087642634 1102 44-45 0.3121508861737894 1102 46-47 -0.3040570537054208 1102 48-49 -0.37197570614277 1102 50-51 -0.09311389268254544 1102 52-53 0.3752029873133722 1102 54-55 0.01921775496440148 1102 56-57 0.15913057777388673 1102 58-59 -0.10380011861767002 1102 60-61 -0.07375017189567501 1102 62-63 0.04648164122530929 1102 64-65 -0.14330125069686517 1102 66-67 0.06294794883776333 1102 68-69 -0.05730525111135876 1102 70-71 -0.03296905711729892 1102 72-73 0.22446321690810578 1102 74-75 0.4146007602438466 1102 76-77 0.5396550778797824 1102 78-79 0.714256727242514 1102 80-81 0.9218221561066322 1102 82-83 0.9376481229888043 1102 84-85 0.9268233709214435 1102 86-87 1.0025580977192199 1102 88-89 1.2486449240488362 1102 90-91 1.2188255101707703 1102 92-93 1.156062215084308 1102 94-95 1.3363242079118756 1102 96-97 1.493517585439907 1102 98-99 1.556853167471921 1102 100-101 1.6738099614344186 1103 1 0.25612390632068127 1103 2 0.2036311228180736 1103 3 0.17173571834924672 1103 4 0.013675734708243681 1103 5 -0.06857546127672265 1103 6 0.08121785860562625 1103 7 0.1285945236097703 1103 8 0.03872508227047433 1103 9 0.3271303962366474 1103 10-11 0.2921087279578387 1103 12-13 0.18347290582857312 1103 14-15 0.47961322090602465 1103 16-17 0.36614187098710715 1103 18-19 0.044780016938446465 1103 20-21 0.23688389459660186 1103 22-23 0.28270186968771327 1103 24-25 0.30219977364729544 1103 26-27 0.30797868158570196 1103 28-29 0.32213592950932224 1103 30-31 0.5375868933313583 1103 32-33 0.4466455986963922 1103 34-35 0.2676861181916408 1103 36-37 0.5802488621917732 1103 38-39 0.7834626916842993 1103 40-41 0.7793197155475795 1103 42-43 0.9635873503040315 1103 44-45 0.772784346410667 1103 46-47 0.6475741857192787 1103 48-49 0.6411839279519782 1103 50-51 0.3991529220382617 1103 52-53 0.3190944318988187 1103 54-55 0.18383545801008694 1103 56-57 0.6538098819193934 1103 58-59 0.6146722798594908 1103 60-61 0.859645291302293 1103 62-63 0.8229727579765829 1103 64-65 0.5369216236686185 1103 66-67 0.5878924285331948 1103 68-69 0.4940378114943975 1103 70-71 0.5129541662329551 1103 72-73 0.8118416010028575 1103 74-75 0.7604261621051016 1103 76-77 0.8245006785565998 1103 78-79 0.9238458769887075 1103 80-81 1.0573511852944506 1103 82-83 1.4603453522612249 1103 84-85 1.7636399424104425 1103 86-87 1.714414326568626 1103 88-89 1.720897990884712 1103 90-91 1.9609931814905686 1103 92-93 1.9891443851350665 1103 94-95 2.3996041359998586 1103 96-97 2.505355307521125 1103 98-99 3.2304929200099934 1103 100-101 3.157960236392121 1104 1 0.1856759337774534 1104 2 0.13202511784993476 1104 3 0.15519925532827727 1104 4 -0.05524219499378802 1104 5 -0.049038914253401344 1104 6 -0.031267290978561846 1104 7 0.07294896553414532 1104 8 0.15764241195570605 1104 9 0.30504377781132774 1104 10-11 0.2666860161219091 1104 12-13 0.19098511617046654 1104 14-15 0.45850162978146614 1104 16-17 0.3474063463924111 1104 18-19 0.4707709446844319 1104 20-21 0.3024447303000031 1104 22-23 0.31678882412244036 1104 24-25 0.4986002728612746 1104 26-27 0.38992897619394995 1104 28-29 0.31820881955024305 1104 30-31 0.4085142792981671 1104 32-33 0.29151725510277515 1104 34-35 0.31981257773251315 1104 36-37 0.33963912511913463 1104 38-39 0.6586621972704592 1104 40-41 0.5358628987567116 1104 42-43 0.6124035171567002 1104 44-45 0.5263574464574745 1104 46-47 0.7945873020865406 1104 48-49 0.8008873996491914 1104 50-51 0.7499527444332941 1104 52-53 0.9010507266750736 1104 54-55 1.2133112833331978 1104 56-57 1.1652162012963387 1104 58-59 1.0287168731080953 1104 60-61 1.1812272955984113 1104 62-63 1.16505433626077 1104 64-65 0.934075743983982 1104 66-67 1.5350039476557278 1104 68-69 1.5599376566900638 1104 70-71 1.640826746665205 1104 72-73 1.7859658764825745 1104 74-75 1.5515873146173718 1104 76-77 1.4299476310712684 1104 78-79 1.374551496746058 1104 80-81 1.6558739439316916 1104 82-83 1.850713282563273 1104 84-85 1.4732828124108046 1104 86-87 1.7324358311707542 1104 88-89 1.9393401072639698 1104 90-91 1.9007754953953544 1104 92-93 2.058932339197785 1104 94-95 2.457094745736935 1104 96-97 2.7945574051798623 1104 98-99 3.212207408015658 1104 100-101 3.0862102273918666 1105 1 0.01299631613946417 1105 2 0.09795172901243632 1105 3 0.1510878358607073 1105 4 0.10654216105513825 1105 5 -0.12777259705739397 1105 6 -0.0884160215777996 1105 7 -0.1295898359870833 1105 8 -0.10286740405827288 1105 9 0.15564507290846308 1105 10-11 -0.04989328288107231 1105 12-13 -5.556382750100397E-4 1105 14-15 0.08821691398333797 1105 16-17 0.26810734341163567 1105 18-19 0.3008922315587199 1105 20-21 0.3224365074610702 1105 22-23 0.3606525305670871 1105 24-25 0.2626818845376988 1105 26-27 0.15020752486287847 1105 28-29 0.13712648837540797 1105 30-31 0.03288471819219296 1105 32-33 0.1504698709934047 1105 34-35 0.24149003687528392 1105 36-37 0.19743540428451922 1105 38-39 0.21072304627857363 1105 40-41 0.04521637804543133 1105 42-43 0.19905168243576554 1105 44-45 0.42801743206749876 1105 46-47 0.37910266851679353 1105 48-49 0.4361274037606009 1105 50-51 0.24954160248653778 1105 52-53 0.5688737300669899 1105 54-55 0.8261697477180263 1105 56-57 0.582345402653111 1105 58-59 0.7192297726866812 1105 60-61 0.7083112713410387 1105 62-63 1.0387823658629962 1105 64-65 0.8519244437475777 1105 66-67 0.9530068256493571 1105 68-69 1.369476149854826 1105 70-71 1.4109470056846334 1105 72-73 1.0826304874395056 1105 74-75 0.8838414003404687 1105 76-77 0.8396248846430652 1105 78-79 0.9442914494647354 1105 80-81 0.7435499640776477 1105 82-83 0.7258803089791392 1105 84-85 1.235077447008397 1105 86-87 1.2668432728399885 1105 88-89 1.4653191390246825 1105 90-91 1.6783477021997548 1105 92-93 1.7676639662920373 1105 94-95 1.9324519253031802 1105 96-97 2.3050929175655135 1105 98-99 2.0332835720612934 1105 100-101 2.598364611192874 1106 1 0.2976818549899747 1106 2 0.3520489794526327 1106 3 0.2718946442944983 1106 4 0.15844066663100875 1106 5 0.04155784675496932 1106 6 0.11185261357503151 1106 7 0.08885117715121282 1106 8 0.2540222975083921 1106 9 0.3806552313806293 1106 10-11 0.1544895334988965 1106 12-13 0.21239979989375257 1106 14-15 0.38191527508315914 1106 16-17 0.40248135578239896 1106 18-19 0.10307856051262121 1106 20-21 0.018336999582615476 1106 22-23 -0.055822253847551906 1106 24-25 0.23674956253670842 1106 26-27 0.06431199910550589 1106 28-29 0.1380012457007922 1106 30-31 2.9859692261169357E-4 1106 32-33 0.11043420346891253 1106 34-35 0.28453938447562876 1106 36-37 0.17423458257698599 1106 38-39 0.2216772654306851 1106 40-41 0.40908156383260064 1106 42-43 0.3089607076739256 1106 44-45 0.5431185653015049 1106 46-47 0.9668492936466038 1106 48-49 1.1098316341998995 1106 50-51 0.9187873923641874 1106 52-53 0.9058810441669536 1106 54-55 1.0572580587027147 1106 56-57 1.1399157218548837 1106 58-59 1.0325170542795234 1106 60-61 0.597358180908067 1106 62-63 0.4860260337486757 1106 64-65 1.1422930834152822 1106 66-67 1.0812744675293509 1106 68-69 1.0553310417235338 1106 70-71 1.2364526718546642 1106 72-73 1.7349285595872317 1106 74-75 1.6922243973154991 1106 76-77 1.6441138006212128 1106 78-79 1.747770161821954 1106 80-81 1.344609831340275 1106 82-83 1.617432975013724 1106 84-85 1.7679570352516585 1106 86-87 1.7898804302737368 1106 88-89 1.7923316452326397 1106 90-91 2.154390638675441 1106 92-93 2.089546444056271 1106 94-95 2.2026842157000353 1106 96-97 2.850811685907196 1106 98-99 2.6653154922382782 1106 100-101 2.2318074302817728 1107 1 0.11840648625187811 1107 2 0.2238875762546897 1107 3 0.237689479746507 1107 4 0.14880696861975906 1107 5 0.05059359109655759 1107 6 0.11363540136791528 1107 7 0.06640576810660548 1107 8 0.15610184749289147 1107 9 0.2555832554759476 1107 10-11 0.2586386675734005 1107 12-13 0.015551237197207968 1107 14-15 0.3242736486491751 1107 16-17 0.4888413681844028 1107 18-19 0.24582892592874828 1107 20-21 0.24301255435074154 1107 22-23 0.29734690060166713 1107 24-25 0.1758856302162073 1107 26-27 0.3756465431104701 1107 28-29 0.33533149329790746 1107 30-31 0.19747935610230627 1107 32-33 0.2592914475668948 1107 34-35 0.25729927285760823 1107 36-37 0.29570222663953416 1107 38-39 0.47739658154885944 1107 40-41 0.5646602501062361 1107 42-43 0.7045236699806949 1107 44-45 1.0248532953365057 1107 46-47 1.1383180450307506 1107 48-49 1.0782674316929288 1107 50-51 1.0536204082653242 1107 52-53 1.0488860935534987 1107 54-55 1.1217429326895143 1107 56-57 1.0039975307754574 1107 58-59 1.3459555093444209 1107 60-61 1.2963438743128606 1107 62-63 1.412743489566541 1107 64-65 1.8920771557012444 1107 66-67 1.9967083161363384 1107 68-69 2.0061327425695303 1107 70-71 1.7730884073376778 1107 72-73 2.1730957208575497 1107 74-75 2.039884626752535 1107 76-77 1.904040938859243 1107 78-79 1.8322588007022311 1107 80-81 2.318537944021326 1107 82-83 2.0980327003979653 1107 84-85 2.1056901264506642 1107 86-87 2.449456547561237 1107 88-89 2.4904868474295654 1107 90-91 2.848620385763187 1107 92-93 3.1107793359373908 1107 94-95 3.203487023867641 1107 96-97 3.123904878093935 1107 98-99 2.905842355860546 1107 100-101 3.105965865857801 1108 1 0.0856980605053721 1108 2 0.0039987055241823555 1108 3 0.014275289752809783 1108 4 0.06261174531062608 1108 5 -0.11366060543415557 1108 6 -0.1112379439499449 1108 7 -0.04020595649825509 1108 8 0.1254946054131878 1108 9 0.06900981910678894 1108 10-11 -0.019598906259858495 1108 12-13 0.06767810199770707 1108 14-15 0.10270966760652556 1108 16-17 0.1864576920468437 1108 18-19 0.07117970686868347 1108 20-21 0.10080327645752618 1108 22-23 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1.1613998695464467 2316 78-79 1.283592664742514 2316 80-81 0.9872518436066322 2316 82-83 1.1860205188221364 2316 84-85 1.1032556625881078 2316 86-87 1.2102403893858842 2316 88-89 1.393501694882172 2316 90-91 1.5684348851707703 2316 92-93 1.688288777584308 2316 94-95 1.9502564995785399 2316 96-97 2.075548835439907 2316 98-99 2.754118792471921 2316 100-101 3.3606589197677543 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 6.0 5 20.0 6 58.0 7 91.0 8 260.0 9 425.0 10 745.0 11 1261.0 12 1890.0 13 2852.0 14 3513.0 15 4760.0 16 6071.0 17 8100.0 18 7442.0 19 5249.0 20 4556.0 21 4696.0 22 4944.0 23 5156.0 24 5928.0 25 6318.0 26 6460.0 27 6620.0 28 7868.0 29 9361.0 30 11454.0 31 13550.0 32 16787.0 33 21564.0 34 31484.0 35 50407.0 36 54280.0 37 79189.0 38 50073.0 39 2176.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.883025416880926 24.784672739293324 16.168138911334754 18.164162932491003 2 14.193712805818823 25.388242965248697 40.210583351700826 20.20746087723165 3 17.204387471189428 31.984554779111303 32.60300640030854 18.20805134939073 4 10.138702686819343 22.440818350443518 40.122082239077336 27.298396723659806 5 8.689546434498007 43.03103294521083 35.73201619909627 12.54740442119489 6 23.021652097259974 43.273892602659224 18.143961654301037 15.560493645779768 7 21.629600381987807 39.22537280540659 23.42866194079189 15.716364871813706 8 23.561793522676144 35.726058969909005 21.588018695536395 19.12412881187846 9 24.765848820979947 19.110638727686773 23.648580401087198 32.47493205024609 10-11 21.776059648865058 30.831512157496512 27.97429387350327 19.418134320135163 12-13 23.792399728200984 30.93033772864174 28.230023690589878 17.047238852567396 14-15 21.078312091383236 31.93064533901418 28.521105744508922 18.46993682509366 16-17 16.156660912363183 35.05690791890105 30.00291541173878 18.783515756996987 18-19 15.531683868361126 32.75224050539925 34.85833853669287 16.857737089546756 20-21 16.989848857709543 31.234389921398666 35.14207467127011 16.633686549621686 22-23 17.013513063699524 31.570731528148105 35.176549043824195 16.239206364328172 24-25 16.818502683003818 32.90653964245746 34.89041292433068 15.38454475020804 26-27 15.844789112647732 32.70119929841961 36.148263955848186 15.305747633084474 28-29 15.016666054506722 32.66925457283479 36.29687614779989 16.01720322485859 30-31 15.876827297436275 32.17214243737604 35.9294654007199 16.021564864467788 32-33 15.381781201792405 33.27380077866745 35.57571347241607 15.768704547124074 34-35 16.08718810553673 32.673269599875105 35.780964450689225 15.458577843898944 36-37 16.242262296248597 31.865278729507207 35.486747908969136 16.405711065275057 38-39 15.380756749807444 32.94098875883436 35.34475930727881 16.333495184079396 40-41 15.274088495331414 32.31746374299483 36.31853360301765 16.08991415865611 42-43 15.477425812583508 32.01296644061104 35.2518216838925 17.25778606291295 44-45 15.472770160526633 31.817905291008326 35.295197975194995 17.414126573270046 46-47 16.630243149930212 31.820112208917944 34.53867626533461 17.010968375817235 48-49 16.18834018952472 31.480592999338867 34.50160232865643 17.82946448247998 50-51 15.064988430177037 31.720712737824137 35.21163134503783 18.002667486960995 52-53 15.746092889150077 32.43441471387644 34.48691048997282 17.33258190700066 54-55 15.869596158084184 31.667454822596046 34.09298556526849 18.369963454051273 56-57 16.32137019760523 31.910903180783073 33.40315782707706 18.364568794534637 58-59 16.052555461691032 31.471066260192465 34.26354862998604 18.212829648130462 60-61 16.311039998530816 32.778066003085286 33.29537941673401 17.615514581649894 62-63 15.491740431940057 32.73341658708587 33.79995225152428 17.97489072944979 64-65 15.521009329317565 32.7365156468082 33.12527547197532 18.61719955189892 66-67 15.594353742745904 32.595336259457866 32.25007805039301 19.560231947403217 68-69 15.482205535647466 31.80919761347802 33.027939937513345 19.680656913361172 70-71 16.397584110776464 31.566792771615365 32.763144604422244 19.272478513185927 72-73 16.230923565709247 30.835529457136563 33.08097039594505 19.85257658120914 74-75 15.637166678909868 32.0243058106222 31.93351483875707 20.405012671710864 76-77 15.724399471093808 31.042362998604272 33.06019521780651 20.173042312495408 78-79 15.763424667597151 30.71535480790421 32.794709101594066 20.726511422904576 80-81 16.297381179754648 30.957655366194082 31.923758539631237 20.82120491442004 82-83 16.13727432482533 30.57187687596345 32.58078623170523 20.71006256750599 84-85 15.659700779845684 30.56905548451672 32.26350381887929 21.507739916758304 86-87 15.630154486821157 30.956018489291292 32.40136959413778 21.012457429749762 88-89 16.031895063542205 30.49933886725924 31.68134320135165 21.78742286784691 90-91 15.929670793843552 29.66620256491781 32.502350160752144 21.901776480486493 92-93 16.53830437816793 30.29078362594579 32.04714702857563 21.12376496731066 94-95 15.929740872695216 29.384251267171084 32.592581539704696 22.093426320429003 96-97 15.887042716520972 29.55469955189892 32.50213490780871 22.056122823771396 98-99 15.695130573716302 29.749481194446485 31.91021450084478 22.645173730992436 100-101 15.674584955557188 28.764209762726807 32.75717604495703 22.80402923675898 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7474.0 1 4357.0 2 1502.5 3 1653.0 4 1234.0 5 1241.5 6 2476.5 7 4465.0 8 5810.5 9 5778.5 10 4246.5 11 2701.5 12 2319.0 13 2103.5 14 1840.0 15 1641.5 16 1497.5 17 1458.0 18 1415.5 19 1437.5 20 1583.0 21 1697.5 22 1788.5 23 1974.5 24 2182.0 25 2636.5 26 3728.5 27 4743.5 28 4976.0 29 5557.5 30 7134.0 31 8581.0 32 10694.0 33 13691.5 34 16297.5 35 18597.5 36 21699.5 37 23637.5 38 22722.5 39 21304.0 40 19847.0 41 18122.0 42 16256.0 43 15724.0 44 15583.0 45 14650.5 46 13044.5 47 12597.5 48 14626.0 49 13579.0 50 9563.5 51 6279.5 52 3971.0 53 2762.5 54 2193.5 55 1753.5 56 1403.0 57 1119.0 58 900.5 59 758.5 60 590.5 61 470.5 62 360.0 63 225.5 64 158.0 65 127.0 66 109.5 67 106.0 68 84.5 69 70.0 70 58.0 71 55.0 72 55.0 73 54.5 74 48.5 75 48.0 76 32.5 77 14.0 78 17.5 79 26.5 80 17.5 81 6.0 82 3.5 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014691838683611255 3 0.00275471975317711 4 0.0018364798354514069 5 0.12166678909865569 6 0.0 7 0.0 8 9.182399177257034E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.1477998971571293E-4 24-25 8.034599280099904E-4 26-27 0.0064276794240799235 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011937118930434145 36-37 0.0012625798868728423 38-39 0.006772019393227063 40-41 0.010330199074414162 42-43 0.006886799382942775 44-45 0.004246859619481378 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 6.886799382942775E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.001492139866304268 84-85 0.0039025196503342393 86-87 0.002869499742892823 88-89 0.0 90-91 0.0017216998457356936 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 435616.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.601761449919863 #Duplication Level Percentage of deduplicated Percentage of total 1 70.2808110569204 16.587509370723254 2 12.036507814994355 5.681655722791858 3 4.678590729877683 3.312689469851386 4 2.5759282653292517 2.4318577772162753 5 1.6472936635193263 1.9439516042173848 6 1.1264231296199148 1.5951341998176831 7 0.9218863771876756 1.5230699650820054 8 0.6574921118526657 1.2414377583320513 9 0.5118261346827585 1.0871998501155316 >10 4.377959157632868 21.652631410384615 >50 0.7642472001022264 12.672930628227252 >100 0.39210071754592035 16.3567413745944 >500 0.0149656762422107 2.537741524776192 >1k 0.009977117494807132 4.267795063101392 >5k 0.003990846997922853 7.1076542807687275 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8834 2.027932858297216 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8516 1.9549327848380225 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7471 1.7150426063321824 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5363 1.2311301696907369 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2625 0.6025949460074929 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2576 0.591346507015353 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2316 0.5316609123631822 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2172 0.4986042753250569 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1717 0.3941544846837582 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1596 0.3663777271725557 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1415 0.32482737089546754 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1287 0.29544369352824507 TruSeq Adapter, Index 11 (95% over 21bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1243 0.28534305443326236 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1177 0.27019209579078823 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 959 0.2201480202747374 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 936 0.21486814074781457 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 800 0.18364798354514067 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 794 0.18227062366855212 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 728 0.167119665026078 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 712 0.1634467053551752 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 709 0.16275802541688092 RNA PCR Primer, Index 11 (95% over 23bp) GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 697 0.16000330566370383 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 695 0.15954418570484097 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 681 0.156330345992801 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 661 0.1517391464041725 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 660 0.15150958642474105 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 619 0.14209762726805258 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 582 0.13360390802908984 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 544 0.12488062881069566 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 494 0.11340262983912436 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 472 0.108352310291633 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 449 0.1030724307647102 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 446 0.10238375082641592 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 446 0.10238375082641592 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 441 0.10123595092925879 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.2955997943142583E-4 0.0 10-11 0.0 0.0 0.0 2.2955997943142583E-4 0.0 12-13 0.0 0.0 0.0 4.5911995886285166E-4 0.0 14-15 0.0 0.0 0.0 6.886799382942775E-4 0.0 16-17 0.0 0.0 0.0 0.0010330199074414163 0.0 18-19 0.0 0.0 0.0 0.001377359876588555 0.0 20-21 0.0 0.0 0.0 0.002295599794314258 0.0 22-23 0.0 0.0 0.0 0.005279879526922794 0.0 24-25 0.0 0.0 0.0 0.014347498714464115 0.0 26-27 0.0 0.0 0.0 0.03294185704840961 0.0 28-29 0.0 0.0 0.0 0.06898277381914347 0.0 30-31 0.0 0.0 0.0 0.15082090648644678 0.0 32-33 0.0 0.0 0.0 0.2675521560273268 0.0 34-35 0.0 0.0 0.0 0.4292771615367663 0.0 36-37 0.0 0.0 0.0 0.6540163814001323 0.0 38-39 0.0 0.0 0.0 0.9463610152060531 0.0 40-41 0.0 0.0 0.0 1.250987107911555 0.0 42-43 0.0 0.0 0.0 1.5334606626019247 0.0 44-45 0.0 0.0 0.0 1.845547454638948 0.0 46-47 0.0 0.0 0.0 2.218467641225299 0.0 48-49 0.0 0.0 0.0 2.566824910012488 0.0 50-51 0.0 0.0 0.0 2.923905458018071 0.0 52-53 0.0 0.0 0.0 3.256423088224491 0.0 54-55 0.0 0.0 0.0 3.611896716374054 0.0 56-57 0.0 0.0 0.0 4.034631418497025 0.0 58-59 0.0 0.0 0.0 4.446921141555865 0.0 60-61 0.0 0.0 0.0 4.854619665026078 0.0 62-63 0.0 0.0 0.0 5.251184529493866 0.0 64-65 0.0 0.0 0.0 5.650963233673695 0.0 66-67 0.0 0.0 0.0 6.103540733122751 0.0 68-69 0.0 0.0 0.0 6.567137111584515 0.0 70-71 0.0 0.0 0.0 7.081121905531477 0.0 72-73 0.0 0.0 0.0 7.549079923602439 0.0 74-75 0.0 0.0 0.0 8.055489238228164 0.0 76-77 0.0 0.0 0.0 8.573032211856315 0.0 78-79 0.0 0.0 0.0 9.170002938367737 0.0 80-81 0.0 0.0 0.0 9.78384632336737 0.0 82-83 0.0 0.0 0.0 10.348563872768677 0.0 84-85 0.0 0.0 0.0 10.915347461984867 0.0 86-87 0.0 0.0 0.0 11.496248989936092 0.0 88-89 0.0 0.0 0.0 12.103320355542497 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 30 1.4933576E-6 47.493114 94-95 GACTGGT 40 5.3883245E-4 47.49311 7 GCAGATC 80 2.2608583E-7 41.556473 8 GTAGATA 105 1.02591E-9 40.70838 6 TGTAGAT 110 1.5952537E-9 38.9116 5 TCCTAAG 80 1.0653326E-5 35.677166 2 GGACTGG 80 1.0771433E-5 35.619835 6 GGACTGA 55 0.002579708 34.540447 6 ATGGGGG 555 0.0 32.56266 5 TGGGGGA 460 0.0 32.006226 6 CCCTATA 150 9.367795E-10 31.71304 2 TGGACTG 90 2.3893153E-5 31.705746 5 ACAATCA 90 2.4119388E-5 31.662075 8 AGCAGAT 125 2.1514461E-7 30.395594 7 TACAATC 95 3.489372E-5 29.995651 7 ATGGGCG 65 0.0058050393 29.266846 5 CAATCAA 100 4.9510352E-5 28.495869 9 GGATATG 135 4.1613384E-7 28.189367 1 CATGGGG 1590 0.0 28.116417 4 CATATGC 85 6.350463E-4 27.978874 3 >>END_MODULE