##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938250_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 431156 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8257799961035 33.0 31.0 34.0 30.0 34.0 2 31.976662739240552 34.0 31.0 34.0 30.0 34.0 3 32.014609561272486 34.0 31.0 34.0 30.0 34.0 4 35.51257085602427 37.0 35.0 37.0 35.0 37.0 5 35.56067409475921 37.0 35.0 37.0 35.0 37.0 6 35.638611082763546 37.0 36.0 37.0 35.0 37.0 7 35.5991914759391 37.0 36.0 37.0 35.0 37.0 8 35.593068402156064 37.0 36.0 37.0 35.0 37.0 9 37.22308862685432 39.0 38.0 39.0 35.0 39.0 10-11 37.181189639016964 39.0 37.5 39.0 35.0 39.0 12-13 37.10941167466068 39.0 37.0 39.0 34.5 39.0 14-15 38.23968934677936 40.0 38.0 41.0 34.0 41.0 16-17 38.14292390689217 40.0 38.0 41.0 34.0 41.0 18-19 38.31301663435045 40.0 38.0 41.0 34.0 41.0 20-21 38.32743021087495 40.0 38.0 41.0 34.5 41.0 22-23 38.22186285242465 40.0 38.0 41.0 34.0 41.0 24-25 38.08102867639555 40.0 38.0 41.0 34.0 41.0 26-27 37.86009472209595 40.0 37.5 41.0 34.0 41.0 28-29 37.63612474371225 40.0 37.0 41.0 33.5 41.0 30-31 37.2961909378508 40.0 36.5 41.0 33.0 41.0 32-33 36.87566913135849 39.0 35.0 41.0 33.0 41.0 34-35 36.59036056554936 39.0 35.0 41.0 32.5 41.0 36-37 36.289000964847986 39.0 35.0 41.0 31.5 41.0 38-39 35.970949030049454 39.0 35.0 41.0 31.0 41.0 40-41 35.69798286467079 38.5 35.0 40.5 30.0 41.0 42-43 35.34478007959996 39.0 35.0 41.0 28.0 41.0 44-45 34.952965979830964 38.5 35.0 41.0 25.0 41.0 46-47 34.43228784940949 38.0 35.0 40.5 21.5 41.0 48-49 33.96154292181948 38.0 35.0 40.0 14.5 41.0 50-51 33.222850893876 37.5 33.5 39.5 7.5 40.5 52-53 33.33346514950505 38.0 34.0 40.0 2.0 40.5 54-55 33.45289060108174 38.0 35.0 40.0 2.0 41.0 56-57 33.184952778112795 38.0 34.0 40.0 2.0 41.0 58-59 32.942019825770714 38.0 34.0 40.0 2.0 41.0 60-61 32.57439418679086 38.0 33.0 40.0 2.0 41.0 62-63 32.12927571459054 37.0 33.0 40.0 2.0 41.0 64-65 31.705771228975127 36.5 32.5 39.5 2.0 41.0 66-67 31.23892512222954 36.0 31.5 39.0 2.0 41.0 68-69 30.712594745289408 35.5 31.0 39.0 2.0 40.0 70-71 30.27722100585403 35.0 30.5 38.5 2.0 40.0 72-73 29.708758546790488 35.0 29.5 37.5 2.0 40.0 74-75 29.10733701954745 35.0 29.0 37.0 2.0 39.0 76-77 28.538191513048645 34.5 28.0 36.5 2.0 39.0 78-79 27.88692955681934 34.0 26.5 36.0 2.0 38.5 80-81 27.27341959754706 34.0 26.0 35.0 2.0 37.0 82-83 26.622033556299808 33.5 24.5 35.0 2.0 37.0 84-85 26.02650432789988 33.0 21.0 35.0 2.0 36.0 86-87 25.47948770282682 33.0 19.0 35.0 2.0 36.0 88-89 24.76222759279704 32.0 11.0 35.0 2.0 35.0 90-91 24.061408167809333 31.5 2.0 35.0 2.0 35.0 92-93 23.27660058076427 31.0 2.0 34.0 2.0 35.0 94-95 22.4674050691629 31.0 2.0 34.0 2.0 35.0 96-97 21.440473981575117 29.5 2.0 34.0 2.0 35.0 98-99 20.21986357606064 29.0 2.0 34.0 2.0 35.0 100-101 16.61214618374788 20.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3360446925424121 1101 2 0.3307975617495629 1101 3 0.3985121738067292 1101 4 0.1992351856678738 1101 5 0.4845701865226175 1101 6 0.4119143855966385 1101 7 0.41469977040700456 1101 8 0.10514489964552354 1101 9 0.17777187055000354 1101 10-11 0.19301325619233012 1101 12-13 0.3613042579339236 1101 14-15 0.31029474161254456 1101 16-17 0.47508055298992957 1101 18-19 0.41809418463548553 1101 20-21 0.3817861971986858 1101 22-23 0.4740802204718051 1101 24-25 0.6035479820011886 1101 26-27 0.5099415088795567 1101 28-29 0.8298224466278938 1101 30-31 0.4713155547317598 1101 32-33 0.37258892866918103 1101 34-35 0.8458502429716148 1101 36-37 0.7122360715557079 1101 38-39 0.7737562028534555 1101 40-41 0.6859602303948407 1101 42-43 0.9763999985001774 1101 44-45 0.8188530558190479 1101 46-47 0.9288830837922077 1101 48-49 1.3896880724206966 1101 50-51 1.1664578735007751 1101 52-53 1.209337450256875 1101 54-55 1.3103407680689685 1101 56-57 1.2249024569145135 1101 58-59 1.1316720198271923 1101 60-61 1.3734494185436077 1101 62-63 1.4439252589458107 1101 64-65 1.1800966435334033 1101 66-67 1.1014363174055042 1101 68-69 1.4550618348463082 1101 70-71 1.5943518052809509 1101 72-73 1.623953710010344 1101 74-75 1.591729398513646 1101 76-77 1.13310961232294 1101 78-79 1.0493627577985265 1101 80-81 1.3076578899970777 1101 82-83 1.2178885464926417 1101 84-85 1.3485106460599212 1101 86-87 1.6811245150084808 1101 88-89 2.1010743468424806 1101 90-91 2.36274625881536 1101 92-93 2.6233449505352233 1101 94-95 2.8185890487004457 1101 96-97 2.7969296448557124 1101 98-99 3.283756649060397 1101 100-101 3.612830291462533 1102 1 0.4230441758800474 1102 2 0.31040231504329086 1102 3 0.23313501028309247 1102 4 0.2847557229967279 1102 5 0.3448646840684688 1102 6 0.35820733315541275 1102 7 0.19670700368006067 1102 8 0.3477243364835516 1102 9 0.569201733634479 1102 10-11 0.46195861914763725 1102 12-13 0.4181597216383963 1102 14-15 0.7032100089853159 1102 16-17 0.5762850221193503 1102 18-19 0.4513478658548067 1102 20-21 0.5481740814663212 1102 22-23 0.6062908895495696 1102 24-25 0.7347059515181158 1102 26-27 0.6839695378126436 1102 28-29 0.5967754303530342 1102 30-31 0.6979494994488888 1102 32-33 0.9473532014669104 1102 34-35 1.0559962000886358 1102 36-37 1.110408378453151 1102 38-39 0.9093477812569688 1102 40-41 0.9076439038642263 1102 42-43 0.81871464587811 1102 44-45 0.9338136603140086 1102 46-47 1.311752497380425 1102 48-49 1.809227597091322 1102 50-51 1.675648005249684 1102 52-53 1.6957007930623504 1102 54-55 1.7848919176427245 1102 56-57 1.8477260167181768 1102 58-59 1.9189718131622442 1102 60-61 1.9728778608065909 1102 62-63 1.8919167340168457 1102 64-65 1.986445132295998 1102 66-67 2.117246185656601 1102 68-69 2.0166383008757585 1102 70-71 2.3012427895227496 1102 72-73 2.5653222194394303 1102 74-75 2.460871738994147 1102 76-77 2.677274556781736 1102 78-79 3.0622341088706015 1102 80-81 2.922999532983386 1102 82-83 2.7706591484042953 1102 84-85 2.8809796463182558 1102 86-87 3.0000408157059724 1102 88-89 3.4867660285784687 1102 90-91 3.5692691211248437 1102 92-93 3.8475312073164147 1102 94-95 3.9183016552620558 1102 96-97 4.284390895178628 1102 98-99 4.7223480982983155 1102 100-101 4.591207971648533 1103 1 0.48879597997327195 1103 2 0.2933984899713238 1103 3 0.44628221640320476 1103 4 0.30679129222264123 1103 5 0.45449834470177564 1103 6 0.4245979637007764 1103 7 0.319795453540074 1103 8 0.4372941144716407 1103 9 0.5073144352394294 1103 10-11 0.37829601415532466 1103 12-13 0.5778032407046041 1103 14-15 0.8136086840119319 1103 16-17 0.9046970257472537 1103 18-19 0.6812307476719113 1103 20-21 0.6508950089476926 1103 22-23 0.7727840477971313 1103 24-25 0.8991603858412347 1103 26-27 0.7382737296183137 1103 28-29 0.8431179517458318 1103 30-31 0.750703945602126 1103 32-33 1.0498848470365303 1103 34-35 0.7985570266196547 1103 36-37 0.8812471339245818 1103 38-39 1.0845912845496812 1103 40-41 1.086148576761417 1103 42-43 1.2954921213306179 1103 44-45 1.464678442048303 1103 46-47 1.2901783028143896 1103 48-49 1.1243859235237466 1103 50-51 1.3359934447386195 1103 52-53 1.3125981470589636 1103 54-55 1.2415069261209695 1103 56-57 1.010378329506544 1103 58-59 1.1572974569179166 1103 60-61 1.2031787401788065 1103 62-63 1.1335193208696381 1103 64-65 1.177974766745308 1103 66-67 1.2952185031770007 1103 68-69 1.4286734757643593 1103 70-71 1.5982379391737602 1103 72-73 1.776414928142458 1103 74-75 1.8154085898163395 1103 76-77 1.6631139037591396 1103 78-79 1.8437791224752402 1103 80-81 2.0457094190199 1103 82-83 1.76882153651108 1103 84-85 1.439795057020568 1103 86-87 1.6951352594931883 1103 88-89 1.9710398564107976 1103 90-91 2.3726801389094554 1103 92-93 2.5383983550746088 1103 94-95 2.942742682116428 1103 96-97 3.2254335230425006 1103 98-99 3.8306962192810055 1103 100-101 3.945019241809028 1104 1 0.28184424524020457 1104 2 0.22304898286595432 1104 3 0.30650071168763304 1104 4 0.30647123077084615 1104 5 0.19503519444359085 1104 6 0.27655887352879915 1104 7 0.09131425181600861 1104 8 0.12467942578474833 1104 9 0.4032517885445941 1104 10-11 0.3712759996458743 1104 12-13 0.3324334630406298 1104 14-15 0.5247318863334414 1104 16-17 0.5514772075421561 1104 18-19 0.5955823031705663 1104 20-21 0.5509398175509332 1104 22-23 0.574740690139123 1104 24-25 0.5747354295829652 1104 26-27 0.41335801246729176 1104 28-29 0.5151616508027175 1104 30-31 0.4278899653179167 1104 32-33 0.41175699315525094 1104 34-35 0.34269350082269767 1104 36-37 0.5846035116824453 1104 38-39 0.5638619711881816 1104 40-41 0.8115251824030381 1104 42-43 0.255591127006376 1104 44-45 0.03980753350052879 1104 46-47 0.15623258523661576 1104 48-49 0.15988073852822993 1104 50-51 0.17900330263133668 1104 52-53 0.12845612512408167 1104 54-55 0.315739845508169 1104 56-57 0.12726477170344452 1104 58-59 -0.019973912518352677 1104 60-61 0.11326280965347735 1104 62-63 0.057323357911421624 1104 64-65 0.3036522301516129 1104 66-67 -0.038392216747887176 1104 68-69 0.21217324105262847 1104 70-71 -0.029157187357199632 1104 72-73 -0.0011103567122354718 1104 74-75 0.22515126040909195 1104 76-77 0.2883432811329314 1104 78-79 0.2612139366262234 1104 80-81 0.07992398821718538 1104 82-83 0.10894017262254962 1104 84-85 0.3564723924022921 1104 86-87 0.6557890383520615 1104 88-89 0.6566434923088877 1104 90-91 1.0377998418924257 1104 92-93 1.4676861116572049 1104 94-95 1.2099726569382625 1104 96-97 1.6608344533544575 1104 98-99 1.9631376480353282 1104 100-101 2.317261378966027 1105 1 0.35063794323760433 1105 2 0.21663203410663812 1105 3 0.20891774481111014 1105 4 0.10609053750837916 1105 5 0.21549350273132006 1105 6 0.25734493656991475 1105 7 0.36974760940403684 1105 8 0.3896514274298468 1105 9 0.29802331985951724 1105 10-11 0.35280552182837255 1105 12-13 0.2525854239800225 1105 14-15 0.2927910888812377 1105 16-17 0.24375366888755678 1105 18-19 0.07786583001418279 1105 20-21 0.09283453108189832 1105 22-23 0.19436642964369355 1105 24-25 0.047045531136319596 1105 26-27 0.1607374508129098 1105 28-29 0.2680853961495089 1105 30-31 0.3838149655815499 1105 32-33 0.34318738745223953 1105 34-35 0.28258140194790826 1105 36-37 0.18444585616239806 1105 38-39 0.18977284045538312 1105 40-41 0.42819817638154234 1105 42-43 0.4531403482197405 1105 44-45 0.6885889986950389 1105 46-47 0.4720471739086207 1105 48-49 0.391953354977133 1105 50-51 0.5389245275989083 1105 52-53 0.44194279031437844 1105 54-55 0.30599578527215243 1105 56-57 0.4563324423035837 1105 58-59 0.7400786041699305 1105 60-61 0.721981845371154 1105 62-63 0.5780453041925426 1105 64-65 0.7761811512394843 1105 66-67 0.8274107718524029 1105 68-69 1.0803357896938373 1105 70-71 0.9004330137458645 1105 72-73 1.0111461736008636 1105 74-75 0.8575186241346131 1105 76-77 0.7460821107911499 1105 78-79 0.7795025042282759 1105 80-81 0.9196347246108267 1105 82-83 1.0093588297560636 1105 84-85 1.3483839787648293 1105 86-87 1.5605313585773146 1105 88-89 1.8245360756448576 1105 90-91 1.8247460702872935 1105 92-93 1.9264214929304728 1105 94-95 2.0755910989431747 1105 96-97 2.3326763338315324 1105 98-99 2.6451796824769964 1105 100-101 2.874436550594364 1106 1 0.11990471232124733 1106 2 -0.09172783607476376 1106 3 0.010049079317830945 1106 4 -0.1374770322589498 1106 5 0.0621122165504886 1106 6 -0.37757585984073927 1106 7 -0.2840101100209367 1106 8 0.06973403926613031 1106 9 0.04423100927157009 1106 10-11 -0.006713661006273242 1106 12-13 -0.006250583385863706 1106 14-15 0.2614657386457111 1106 16-17 0.183595566925014 1106 18-19 0.1089037683808769 1106 20-21 -0.1447635196043322 1106 22-23 -0.02934174302724557 1106 24-25 0.21078636193465883 1106 26-27 0.20814006142833108 1106 28-29 0.07628248438749807 1106 30-31 -0.14155866984793875 1106 32-33 0.32962052985590873 1106 34-35 0.18870963342383362 1106 36-37 0.306758682362144 1106 38-39 0.03376923890488115 1106 40-41 0.24178487302721408 1106 42-43 -0.06450623183337001 1106 44-45 0.0048921190214201715 1106 46-47 0.2434733472103474 1106 48-49 0.11877981925158565 1106 50-51 0.19000143189330032 1106 52-53 0.1785285407900048 1106 54-55 -0.24527035303145084 1106 56-57 0.19696485015374776 1106 58-59 0.56520501452286 1106 60-61 0.5140533473398037 1106 62-63 0.34319928573714265 1106 64-65 0.5361718930164585 1106 66-67 0.7755799836825794 1106 68-69 0.22819520713795782 1106 70-71 0.5383754499811211 1106 72-73 0.9202935014335196 1106 74-75 0.6462785309419523 1106 76-77 1.100143011585729 1106 78-79 1.1082609866936082 1106 80-81 0.7678024103602858 1106 82-83 0.7423760948159384 1106 84-85 0.7807175596623672 1106 86-87 0.5062054284311159 1106 88-89 1.1147228679249253 1106 90-91 1.2538952294168162 1106 92-93 1.54979240176241 1106 94-95 2.0046843017796476 1106 96-97 2.0443055776076697 1106 98-99 2.752262223096178 1106 100-101 3.4707373309303016 1107 1 0.033198887553748335 1107 2 0.05653452320080987 1107 3 0.19274119875004203 1107 4 0.29277260063386024 1107 5 0.16556791894892342 1107 6 0.10493025861251226 1107 7 -0.05657007086283272 1107 8 -0.024118138903226338 1107 9 0.14937051000578094 1107 10-11 -0.1625139546920309 1107 12-13 6.069790223648397E-4 1107 14-15 0.11858272347195253 1107 16-17 0.010335655030743851 1107 18-19 0.17205110073525276 1107 20-21 0.1906354035478941 1107 22-23 0.27737387126546054 1107 24-25 0.29490285728463306 1107 26-27 0.20140976284780976 1107 28-29 0.3264097482151982 1107 30-31 0.3658960535979787 1107 32-33 0.24655151370319572 1107 34-35 0.16173459671311008 1107 36-37 0.11714536860786495 1107 38-39 0.2410918037604901 1107 40-41 -0.07346720724689249 1107 42-43 0.18005079215097197 1107 44-45 0.1908882032113297 1107 46-47 0.20891142846340216 1107 48-49 0.47905882072001305 1107 50-51 0.20315855377289438 1107 52-53 -0.05393352479980962 1107 54-55 0.007353239724302796 1107 56-57 0.07667116439750288 1107 58-59 0.21836703116505873 1107 60-61 0.279305427613906 1107 62-63 0.371691698855102 1107 64-65 0.40153655283045 1107 66-67 0.490846748244504 1107 68-69 0.570407639834972 1107 70-71 0.5438869526732439 1107 72-73 0.757474118173608 1107 74-75 0.9143597840011779 1107 76-77 1.2759384105088785 1107 78-79 1.3629795378438772 1107 80-81 1.1509742165276897 1107 82-83 1.1549066870822138 1107 84-85 0.8102623467401919 1107 86-87 0.9939929957341 1107 88-89 1.2504791087472427 1107 90-91 1.2482564628969932 1107 92-93 1.0000909822812538 1107 94-95 1.3818600237571346 1107 96-97 1.4804387151504983 1107 98-99 1.231630798720257 1107 100-101 1.5447804048412195 1108 1 0.21792701994145247 1108 2 0.11492695818898824 1108 3 0.12314309000299772 1108 4 0.17646054389626897 1108 5 0.11696271800329328 1108 6 0.18303900565742737 1108 7 0.15770888894803647 1108 8 -0.02189591668100377 1108 9 0.1245478149703203 1108 10-11 0.11124491055620211 1108 12-13 0.055784283986902494 1108 14-15 0.18101771165162006 1108 16-17 0.3056548039669096 1108 18-19 0.3042969636194144 1108 20-21 0.3058363491743705 1108 22-23 -0.03910367010570326 1108 24-25 -0.017744896852477154 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-3.0094117299242598 2316 96-97 -3.070873199173466 2316 98-99 -3.5416623739705066 2316 100-101 -3.3345100904733727 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4612.0 3 660.0 4 490.0 5 453.0 6 447.0 7 493.0 8 561.0 9 782.0 10 934.0 11 1429.0 12 2066.0 13 3012.0 14 3758.0 15 5146.0 16 6304.0 17 7885.0 18 6877.0 19 4598.0 20 4238.0 21 4136.0 22 4287.0 23 4516.0 24 4759.0 25 5405.0 26 5878.0 27 6515.0 28 7685.0 29 8903.0 30 10689.0 31 12800.0 32 15865.0 33 20663.0 34 30312.0 35 49477.0 36 53068.0 37 75686.0 38 52279.0 39 3488.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.17188603061955 24.17737061483727 15.930457149647136 17.720286204896052 2 14.35336646666017 25.428204164404413 40.21940387854826 19.999025490387158 3 17.53639734718219 31.964458924485655 32.09301570063443 18.40612802769772 4 10.473233265569448 21.86104690560398 40.74883733882871 26.916882489997867 5 9.123891052751304 42.52438407294099 35.42363990028179 12.928084974025914 6 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36.53162691667295 15.3426660246332 28-29 15.416751744876745 32.52327275764776 36.37371918621919 15.686256311256312 30-31 16.04683890743248 32.20958009497258 35.836660158040125 15.906920839554815 32-33 15.704860099984105 32.846020068752125 35.79298719047912 15.656132640784651 34-35 16.4161337330722 32.28510168280164 35.729716810380836 15.569047773745323 36-37 16.422353734188302 31.55262367145779 35.758363971547936 16.26665862280597 38-39 15.576591383868038 32.91719201463357 35.5178529381402 15.988363663358182 40-41 15.606259902890773 32.18013227826224 36.310986148132166 15.902621670714817 42-43 15.559896210909455 32.17148630199749 35.0869297428587 17.18168774423436 44-45 15.838418187870346 31.639258131224622 35.32262198721644 17.199701693688596 46-47 16.561245428298673 32.023194605711424 34.87264194168406 16.54291802430585 48-49 16.209607635255992 31.740992406966196 34.70559304772267 17.343806910055143 50-51 15.293541824693746 31.781130464837382 35.162967963126626 17.762359747342245 52-53 15.837777401478547 32.558646621773654 34.402538628018156 17.201037348729642 54-55 16.048031009298384 31.65474551521527 34.433009778553064 17.864213696933284 56-57 16.342981077585957 31.63632778747514 34.04677541883891 17.9739157161 58-59 16.509802147739123 30.8787080626623 34.52387994094239 18.087609848656182 60-61 16.55826080501791 31.747337020060495 34.286446638335775 17.407955536585817 62-63 16.013078338972083 31.35136263626653 34.77416204567676 17.86139697908463 64-65 16.15681626015883 31.479584913144688 34.13308806387521 18.230510762821265 66-67 16.259898590190247 31.40387175787828 33.2397789952728 19.09645065665867 68-69 16.21447650285139 30.71023218214567 33.96396009793426 19.111331217068674 70-71 17.023611106278395 30.78351479217193 33.90050094006001 18.292373161489667 72-73 17.071716705443528 29.944861853077832 34.20606403633504 18.7773574051436 74-75 16.47346183242849 31.270071942946682 33.13458074647621 19.12188547814862 76-77 16.864658488751985 30.33281878268373 33.64175445148167 19.160768277082628 78-79 17.059903526199747 30.03666189980782 33.49767978074769 19.405754793244746 80-81 17.81398353690857 30.07883586898655 32.60420496630019 19.50297562780469 82-83 17.847657310134142 29.71037352675786 33.077750999171904 19.3642181639361 84-85 17.4962915368173 29.772203517212027 32.90876890620343 19.822736039767253 86-87 17.830436375950384 29.787053917270878 32.87559551145562 19.50691419532312 88-89 18.479075367651323 29.25922231218405 32.60143325437829 19.66026906578633 90-91 18.770430759879268 28.6137359667215 32.783989012340236 19.831844261059 92-93 19.66380492195347 29.015006182018098 32.588050263402685 18.733138632625746 94-95 19.35122393724755 28.016342363703433 33.014521132086855 19.61791256696216 96-97 19.4401397504721 28.012035615027354 32.70715502002069 19.840669614479857 98-99 19.573364449666585 28.298387386749962 32.003929751629954 20.1243184119535 100-101 20.35620336227171 26.96298016898464 32.60869312737725 20.072123341366392 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7414.0 1 4385.0 2 1629.5 3 1733.5 4 1245.5 5 1287.0 6 2473.0 7 4448.0 8 6041.5 9 6098.5 10 4350.5 11 2691.0 12 2318.0 13 2100.0 14 1840.5 15 1599.0 16 1460.5 17 1509.0 18 1561.0 19 1530.0 20 1614.0 21 1803.5 22 1913.0 23 2026.5 24 2250.5 25 2684.0 26 3647.0 27 4671.5 28 4894.5 29 5350.0 30 6924.5 31 8504.0 32 10553.5 33 13438.0 34 16184.0 35 18425.0 36 21150.5 37 23021.5 38 22238.5 39 20765.0 40 19085.5 41 17271.5 42 15632.0 43 14932.5 44 14366.0 45 13543.5 46 12360.0 47 11757.5 48 13608.5 49 12937.5 50 9140.0 51 6207.0 52 4514.5 53 3608.0 54 2891.0 55 2247.0 56 1765.0 57 1388.0 58 1198.5 59 1098.5 60 909.5 61 733.5 62 595.5 63 452.5 64 350.0 65 338.0 66 318.5 67 291.5 68 259.0 69 208.5 70 184.5 71 160.0 72 137.5 73 125.5 74 109.0 75 94.0 76 78.5 77 52.5 78 33.5 79 32.0 80 29.0 81 20.0 82 10.0 83 5.0 84 1.5 85 1.0 86 1.5 87 1.5 88 1.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45296829917709597 2 0.03942888420896381 3 0.05056174563267124 4 0.05751978402248838 5 0.07932163764391542 6 0.07653842228798857 7 0.04058855727393333 8 0.03571793040106133 9 0.04035662266093943 10-11 0.04290790340387238 12-13 0.038385178450491234 14-15 0.03281874773863752 16-17 0.034674224642588757 18-19 0.046618857211774854 20-21 0.05322899368210114 22-23 0.04696675913126572 24-25 0.06250637820185732 26-27 0.05415673213407676 28-29 0.04174823033890286 30-31 0.04395160916234495 32-33 0.04348773993635714 34-35 0.04824239950273219 36-37 0.04302387071036933 38-39 0.02145395170193619 40-41 0.022033788234420953 42-43 0.020758147862954476 44-45 0.012872371021161714 46-47 0.02493297089684476 48-49 0.023889265138372192 50-51 0.013684142166640382 52-53 0.01322027294065257 54-55 0.013800109473137333 56-57 0.012408501795173905 58-59 0.011944632569186096 60-61 0.013916076779634285 62-63 0.014263978699125143 64-65 0.015191717151100763 66-67 0.007885776841792761 68-69 0.01171269795619219 70-71 0.015423651764094666 72-73 0.01322027294065257 74-75 0.01171269795619219 76-77 0.008233678761283619 78-79 0.01264043640816781 80-81 0.016351390216070287 82-83 0.011364796036701333 84-85 0.011016894117210477 86-87 0.003247084581914667 88-89 0.02528087281633562 90-91 0.029455695850225904 92-93 0.015539619070591617 94-95 0.029919565076213715 96-97 0.023657330525378283 98-99 0.019830409410978855 100-101 0.1492499234615777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 431156.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.144155445851116 #Duplication Level Percentage of deduplicated Percentage of total 1 75.96220053760067 21.378919799391483 2 9.814253358261798 5.524277441997729 3 3.7709192488516727 3.1838801254030082 4 2.01549409074547 2.268975159605395 5 1.3141010460157234 1.8492132055311035 6 0.9323451283847835 1.5744039733466018 7 0.6773288477002131 1.3343993864343813 8 0.5552162075672428 1.2500873001462738 9 0.42486623030464044 1.0761751106447923 >10 3.626620271482243 21.500718830483613 >50 0.5725373912296482 11.240964364055626 >100 0.31266811423060037 15.04089047596818 >500 0.011549745644402126 2.2721542790649063 >1k 0.006599854653944071 3.4172866011932648 >5k 0.0032999273269720356 7.087653946733632 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9254 2.146322908645595 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8392 1.9463952722448485 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7405 1.7174758092198645 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5475 1.2698420061416285 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2807 0.6510404586738907 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2566 0.5951442169423596 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2212 0.5130393639425173 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1712 0.39707205744556495 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1574 0.3650650808524061 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1404 0.3256361966434423 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1223 0.2836560316915455 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1220 0.2829602278525638 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 927 0.2150033862453497 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 906 0.21013275937247772 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 778 0.1804451289092579 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 759 0.1760383712623737 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 744 0.17255935206746514 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 717 0.1662971175166297 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 710 0.1646735752256724 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 688 0.15957101373980648 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 620 0.14379946005622096 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 617 0.14310365621723922 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 609 0.141248179313288 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 577 0.13382627169748304 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 567 0.13150692556754398 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 567 0.13150692556754398 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 498 0.11550343727096458 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 467 0.10831346426815353 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 458 0.10622605275120837 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 450 0.10437057584725715 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1596730649695238E-4 0.0 12-13 0.0 0.0 0.0 3.479019194908571E-4 0.0 14-15 0.0 0.0 0.0 4.638692259878095E-4 0.0 16-17 0.0 0.0 0.0 5.798365324847618E-4 0.0 18-19 1.1596730649695238E-4 0.0 0.0 6.958038389817142E-4 0.0 20-21 2.3193461299390476E-4 0.0 0.0 0.0011596730649695236 0.0 22-23 2.3193461299390476E-4 0.0 0.0 0.004290790340387238 0.0 24-25 2.3193461299390476E-4 0.0 0.0 0.014032044086131238 0.0 26-27 2.3193461299390476E-4 0.0 0.0 0.03513809386857657 0.0 28-29 2.3193461299390476E-4 0.0 0.0 0.0703921550436501 0.0 30-31 2.3193461299390476E-4 0.0 0.0 0.1463507407991539 0.0 32-33 2.3193461299390476E-4 0.0 0.0 0.26788447800796 0.0 34-35 2.3193461299390476E-4 0.0 0.0 0.43603707242854095 0.0 36-37 2.3193461299390476E-4 0.0 0.0 0.6682036200354395 0.0 38-39 2.3193461299390476E-4 0.0 0.0 0.9733136034289214 0.0 40-41 2.3193461299390476E-4 0.0 0.0 1.28538162521222 0.0 42-43 2.3193461299390476E-4 0.0 0.0 1.563355258885415 0.0 44-45 2.3193461299390476E-4 0.0 0.0 1.8746115095232352 0.0 46-47 2.3193461299390476E-4 0.0 0.0 2.2661171362569466 0.0 48-49 2.3193461299390476E-4 0.0 0.0 2.608684559648944 0.0 50-51 2.3193461299390476E-4 0.0 0.0 2.9672554713375208 0.0 52-53 2.3193461299390476E-4 0.0 0.0 3.320491886927237 0.0 54-55 2.3193461299390476E-4 0.0 0.0 3.688688085055061 0.0 56-57 2.3193461299390476E-4 0.0 0.0 4.097820742376309 0.0 58-59 2.3193461299390476E-4 0.0 0.0 4.498835688242771 0.0 60-61 2.3193461299390476E-4 0.0 0.0 4.9050691629015954 0.0 62-63 2.3193461299390476E-4 0.0 0.0 5.292515933907913 0.0 64-65 2.3193461299390476E-4 0.0 0.0 5.69863344126024 0.0 66-67 2.3193461299390476E-4 0.0 0.0 6.1195947638441766 0.0 68-69 2.3193461299390476E-4 0.0 0.0 6.599119576209075 0.0 70-71 2.3193461299390476E-4 0.0 0.0 7.114478286281532 0.0 72-73 2.3193461299390476E-4 0.0 0.0 7.579159283414819 0.0 74-75 2.3193461299390476E-4 0.0 0.0 8.068309382218965 0.0 76-77 2.3193461299390476E-4 0.0 0.0 8.571259590496247 0.0 78-79 2.3193461299390476E-4 0.0 0.0 9.15677852099936 0.0 80-81 2.3193461299390476E-4 0.0 0.0 9.75760513596007 0.0 82-83 2.3193461299390476E-4 0.0 0.0 10.32039447438978 0.0 84-85 2.3193461299390476E-4 0.0 0.0 10.884227518577962 0.0 86-87 2.3193461299390476E-4 0.0 0.0 11.459193424189852 0.0 88-89 2.3193461299390476E-4 0.0 0.0 12.058396496859604 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGTGG 15 6.592957E-4 95.08014 2 GTAAGCG 70 0.0 40.76994 94-95 ATATCTA 100 1.2881774E-6 33.27805 5 GGATATG 130 7.8125595E-9 33.073692 1 CCTTTAG 135 1.1286829E-8 31.848742 1 CGACGGC 30 0.003924078 31.709951 94-95 CCCTATA 75 3.060103E-4 31.693378 2 GGGGAGC 60 0.003947645 31.667635 7 GCTCTTA 60 0.003947645 31.667635 8 GATTGGC 155 1.3515091E-9 30.6461 9 TAGGGTC 110 2.7103433E-6 30.25277 4 GGTACTC 80 4.466911E-4 29.712543 3 GTATAGG 220 1.8189894E-12 28.229563 1 CTTTAGG 135 4.183803E-7 28.171892 2 CGTATCT 35 0.0083347 27.17996 94-95 GCGCCGT 70 5.7344187E-7 27.17996 94-95 AGGGTCT 105 6.856503E-5 27.165752 5 ACTCCCG 70 0.0083608925 27.159445 6 CATTCCG 140 5.790389E-7 27.143686 9 GACGACG 35 0.008389176 27.143686 92-93 >>END_MODULE