##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938250_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 431156 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44534228910186 33.0 31.0 34.0 31.0 34.0 2 32.651717707743835 34.0 31.0 34.0 31.0 34.0 3 32.57538570726141 34.0 31.0 34.0 31.0 34.0 4 36.13499986083923 37.0 35.0 37.0 35.0 37.0 5 36.12839900175342 37.0 35.0 37.0 35.0 37.0 6 36.220940912337994 37.0 37.0 37.0 35.0 37.0 7 36.18341157260945 37.0 37.0 37.0 35.0 37.0 8 36.2054708736513 37.0 37.0 37.0 35.0 37.0 9 37.86149792650456 39.0 38.0 39.0 35.0 39.0 10-11 37.85644291161436 39.0 38.0 39.0 35.0 39.0 12-13 37.812947053966546 39.0 38.0 39.0 35.0 39.0 14-15 38.959519292321104 40.0 38.0 41.0 35.5 41.0 16-17 38.910788670458025 40.0 38.0 41.0 35.5 41.0 18-19 38.98207493343476 40.0 38.5 41.0 35.5 41.0 20-21 39.02684991047324 40.0 38.5 41.0 35.0 41.0 22-23 38.96793735909972 40.0 38.0 41.0 35.0 41.0 24-25 38.86427186447597 40.0 38.0 41.0 35.0 41.0 26-27 38.61059917987921 40.0 38.0 41.0 35.0 41.0 28-29 38.48533361474733 40.0 38.0 41.0 35.0 41.0 30-31 38.12295317704033 40.0 37.0 41.0 34.0 41.0 32-33 37.72944479492341 40.0 35.0 41.0 33.5 41.0 34-35 37.42231814006995 40.0 35.0 41.0 33.0 41.0 36-37 37.187630463219804 40.0 35.0 41.0 33.0 41.0 38-39 36.9822164135487 40.0 35.0 41.0 33.0 41.0 40-41 36.81496024640734 40.0 35.0 41.0 33.0 41.0 42-43 36.514355592871254 40.0 35.0 41.0 32.0 41.0 44-45 36.133327612279544 40.0 35.0 41.0 31.0 41.0 46-47 35.600272291235655 39.0 35.0 41.0 28.5 41.0 48-49 35.09597338318382 39.0 35.0 41.0 24.5 41.0 50-51 34.75855838721948 39.0 35.0 41.0 23.5 41.0 52-53 34.3751159673065 38.5 35.0 40.0 21.0 41.0 54-55 34.06911651467219 38.0 35.0 40.0 19.0 41.0 56-57 33.784139615359635 38.0 34.0 40.0 13.0 41.0 58-59 33.41544359814081 38.0 34.0 40.0 5.0 41.0 60-61 32.973988533152735 38.0 33.0 40.0 2.0 41.0 62-63 32.55459856757183 37.0 33.0 40.0 2.0 41.0 64-65 32.12216691870228 36.5 33.0 39.5 2.0 41.0 66-67 31.56779216803199 36.0 32.0 39.0 2.0 40.5 68-69 30.99219655994582 35.5 31.0 39.0 2.0 40.0 70-71 30.421920372208668 35.0 30.5 38.0 2.0 40.0 72-73 29.961557997569322 35.0 30.0 37.5 2.0 39.5 74-75 29.418359016226145 35.0 29.0 37.0 2.0 39.0 76-77 28.134867658109826 33.5 27.5 35.5 2.0 38.0 78-79 28.776730464147548 34.0 29.0 36.0 2.0 38.0 80-81 28.5762566217332 34.0 29.0 36.0 2.0 37.5 82-83 28.16256528959356 34.0 28.5 35.0 2.0 37.0 84-85 27.683680616760522 34.0 27.5 35.0 2.0 36.5 86-87 27.26609742181484 34.0 26.0 35.0 2.0 36.0 88-89 26.73682727365501 33.5 25.5 35.0 2.0 35.5 90-91 26.288762072196604 33.0 24.5 35.0 2.0 35.0 92-93 25.795925372718926 33.0 22.5 35.0 2.0 35.0 94-95 25.194731141396616 32.5 19.0 35.0 2.0 35.0 96-97 24.549363803356556 32.0 7.5 35.0 2.0 35.0 98-99 23.79838619896279 32.0 2.0 34.0 2.0 35.0 100-101 20.678995537578047 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04496394961324057 1101 2 0.06468859120194992 1101 3 0.2535298383443134 1101 4 0.06353779495296408 1101 5 -0.045848325331760975 1101 6 0.006946522243481468 1101 7 0.10687797839145219 1101 8 0.04869350982458087 1101 9 -0.10182584656698879 1101 10-11 0.0013711993175391513 1101 12-13 -0.021295569397786096 1101 14-15 0.07845028895960127 1101 16-17 0.2345112222234249 1101 18-19 0.26178773848064196 1101 20-21 0.13178698817923618 1101 22-23 0.1957914350641019 1101 24-25 0.15955185037942243 1101 26-27 0.10992170937741719 1101 28-29 0.1595913143555734 1101 30-31 0.17091533604934028 1101 32-33 0.10576449439887625 1101 34-35 -0.049985719027226594 1101 36-37 0.2186041855182168 1101 38-39 0.3960535575750015 1101 40-41 0.14484546097310158 1101 42-43 0.2516028930061509 1101 44-45 0.6524300443368247 1101 46-47 0.8995222464291643 1101 48-49 0.8509782006722375 1101 50-51 0.7914597255350699 1101 52-53 0.6236184133965423 1101 54-55 0.5730070123261299 1101 56-57 0.638481919531948 1101 58-59 0.5591698298644232 1101 60-61 0.5712632805289743 1101 62-63 0.9242406667942333 1101 64-65 0.8412029476836551 1101 66-67 1.0932529453579924 1101 68-69 1.041106416109045 1101 70-71 0.9782602803947533 1101 72-73 0.8586057339366739 1101 74-75 1.0169100603958583 1101 76-77 1.0481755959173036 1101 78-79 0.9623343966406424 1101 80-81 1.0064046617436766 1101 82-83 0.9925943360379357 1101 84-85 1.3184974477093903 1101 86-87 1.166765350321814 1101 88-89 1.7120390073679523 1101 90-91 1.7012806517361945 1101 92-93 2.1593118212133326 1101 94-95 1.9577963869193304 1101 96-97 2.0245366208409905 1101 98-99 2.223477691931091 1101 100-101 2.7572802814400887 1102 1 0.056356354676530884 1102 2 0.10728740287851934 1102 3 0.333270215507838 1102 4 0.22399374948667372 1102 5 0.11290910168968082 1102 6 0.0963614021194843 1102 7 0.13754188952552937 1102 8 0.008761967587432196 1102 9 0.07713307877529729 1102 10-11 0.23405332279984492 1102 12-13 0.19924175945779865 1102 14-15 0.2895908211218341 1102 16-17 0.4055200054835666 1102 18-19 0.3889835535155157 1102 20-21 0.3215796774068238 1102 22-23 0.5653793968310836 1102 24-25 0.4656387788216847 1102 26-27 0.46014452002065553 1102 28-29 0.36464168893578375 1102 30-31 0.5241799963438396 1102 32-33 0.6181353288085845 1102 34-35 0.45947566046127264 1102 36-37 0.6558167920798326 1102 38-39 0.9168201553533493 1102 40-41 0.7307857864703919 1102 42-43 0.5680036938328143 1102 44-45 0.6764064070338023 1102 46-47 0.928180861774031 1102 48-49 1.0559349301994914 1102 50-51 1.4105697746180041 1102 52-53 1.4077407332105452 1102 54-55 1.3485656535041173 1102 56-57 1.5815489564733056 1102 58-59 1.370165050737576 1102 60-61 1.656370487968914 1102 62-63 1.4427242627642656 1102 64-65 1.4764231750150927 1102 66-67 1.6040318138674259 1102 68-69 1.9882098777468684 1102 70-71 2.112689368485686 1102 72-73 1.8708506318958555 1102 74-75 1.9914676682491255 1102 76-77 1.8981077839824074 1102 78-79 1.8738172176171375 1102 80-81 2.0204320448488176 1102 82-83 1.869399425162193 1102 84-85 1.9976824128346848 1102 86-87 2.3388106874440027 1102 88-89 2.1710095576133703 1102 90-91 2.0004087840017597 1102 92-93 2.186791800546839 1102 94-95 2.475301553542945 1102 96-97 2.30573011089524 1102 98-99 2.5721375989318638 1102 100-101 3.143084983067574 1103 1 0.3642785913577882 1103 2 0.3931582968412144 1103 3 0.3227729561521855 1103 4 0.18400794563005718 1103 5 0.11889514214868768 1103 6 0.17242907040293431 1103 7 0.24086615310059045 1103 8 0.17733328349105903 1103 9 0.2274544636879483 1103 10-11 0.2795953394408812 1103 12-13 0.26534058038922836 1103 14-15 0.4019848429681261 1103 16-17 0.42979541066515736 1103 18-19 0.42668220093852227 1103 20-21 0.32364994831656446 1103 22-23 0.37066746024051866 1103 24-25 0.40492266225557216 1103 26-27 0.4017195032613259 1103 28-29 0.43514920106165533 1103 30-31 0.44422021875008255 1103 32-33 0.25484024619492374 1103 34-35 0.13189965194359132 1103 36-37 0.1509743298376236 1103 38-39 0.33904500649101976 1103 40-41 0.1939665112623743 1103 42-43 0.04188279001167672 1103 44-45 -0.05984107906581215 1103 46-47 -0.16354199796018065 1103 48-49 -0.1459184551863686 1103 50-51 -0.09892586795465519 1103 52-53 -0.5884381381960608 1103 54-55 -0.09370336341294916 1103 56-57 -0.1474111760240291 1103 58-59 -0.17069618196087077 1103 60-61 0.10726366198996828 1103 62-63 -0.22152511615446002 1103 64-65 -0.38439546925321366 1103 66-67 0.06698776638920734 1103 68-69 0.10443291493287177 1103 70-71 0.07061357685352121 1103 72-73 0.111037547783706 1103 74-75 0.07197951808547387 1103 76-77 -0.1422171499660969 1103 78-79 0.0443326164893314 1103 80-81 0.08698829706696287 1103 82-83 0.4090027218221586 1103 84-85 0.5451409088321597 1103 86-87 0.5125478221890631 1103 88-89 0.41418949373072067 1103 90-91 0.6643663533065443 1103 92-93 1.0796897854831542 1103 94-95 1.072612173046867 1103 96-97 1.2975673284511409 1103 98-99 1.5275617087153748 1103 100-101 2.3664258088099146 1104 1 -0.04864335632283456 1104 2 0.059376877475365575 1104 3 0.21158360779730145 1104 4 0.2016540003392251 1104 5 0.10150513660095584 1104 6 0.19999703011786352 1104 7 0.16255778456049796 1104 8 0.001086110700548204 1104 9 0.18027740569282003 1104 10-11 0.16638960393965618 1104 12-13 -0.005570101128292038 1104 14-15 -0.12594585305885175 1104 16-17 0.11368138343859613 1104 18-19 0.22270703770718114 1104 20-21 0.2958152707251287 1104 22-23 0.2600328850687603 1104 24-25 0.2737828167385743 1104 26-27 0.13811689155988205 1104 28-29 0.12250250622957282 1104 30-31 0.12359332505315734 1104 32-33 -0.07362815307106985 1104 34-35 0.08731509170802809 1104 36-37 -0.03356243455446872 1104 38-39 -0.03406129658584689 1104 40-41 -8.655631625771321E-4 1104 42-43 -0.31955193878958 1104 44-45 -0.4064356252186698 1104 46-47 -0.10106600256906262 1104 48-49 -0.34227615586490145 1104 50-51 -0.13987239635477522 1104 52-53 -0.2774450941982778 1104 54-55 -0.13368970745766973 1104 56-57 -0.1876002256548972 1104 58-59 -0.28764663644813027 1104 60-61 -0.4398493837148081 1104 62-63 -0.46057901450294025 1104 64-65 -0.660736526736251 1104 66-67 -0.42131932190507726 1104 68-69 -0.37109305271957993 1104 70-71 -0.4737362716507221 1104 72-73 -0.3556333863689858 1104 74-75 0.00489463778845689 1104 76-77 -0.06235114902724703 1104 78-79 -0.230098740997974 1104 80-81 -0.15236432530319988 1104 82-83 -0.20181322150562764 1104 84-85 0.12807949970827437 1104 86-87 -0.040187636352307265 1104 88-89 -0.08857370346980531 1104 90-91 0.09447560094875485 1104 92-93 -0.1882443245326364 1104 94-95 0.05944697866287996 1104 96-97 0.32574340492448783 1104 98-99 0.718264990412127 1104 100-101 1.4407440779175857 1105 1 0.152354249844187 1105 2 0.08846669366695892 1105 3 0.22073184616822772 1105 4 0.17561183935131908 1105 5 0.1274207513520338 1105 6 0.24641840799547765 1105 7 0.17816515376969022 1105 8 0.1582459913252663 1105 9 0.28250040125315223 1105 10-11 0.10667296205496513 1105 12-13 0.12966828034534217 1105 14-15 0.14459418300682358 1105 16-17 0.35420887033578197 1105 18-19 0.2009226303126681 1105 20-21 0.19236635849549089 1105 22-23 0.2315705273014501 1105 24-25 0.05722529620116745 1105 26-27 0.2726302685516586 1105 28-29 0.360738978964136 1105 30-31 0.16023109699575144 1105 32-33 0.0948681612697797 1105 34-35 0.5060012254041268 1105 36-37 0.5274249424584099 1105 38-39 0.5976047900772414 1105 40-41 0.7609375098018774 1105 42-43 0.7563072574308976 1105 44-45 0.9236365061363259 1105 46-47 0.6340305416641172 1105 48-49 0.7930676808060966 1105 50-51 0.9781319694903914 1105 52-53 0.8032650411007367 1105 54-55 1.0789132431787465 1105 56-57 0.8905763485275671 1105 58-59 0.9930170985640459 1105 60-61 1.1365529067720885 1105 62-63 1.2043344595076206 1105 64-65 1.1236445039828524 1105 66-67 1.2476515408238917 1105 68-69 0.9813223985759336 1105 70-71 0.9028785110588409 1105 72-73 0.9419822716187198 1105 74-75 1.0003284277427973 1105 76-77 1.3192701191769558 1105 78-79 1.5600744164360911 1105 80-81 1.7182395111568844 1105 82-83 1.5721620176140298 1105 84-85 1.5124162392444802 1105 86-87 1.6434968627882327 1105 88-89 1.8267642277101324 1105 90-91 1.8266694908746217 1105 92-93 2.2378632186776315 1105 94-95 2.4254608776578905 1105 96-97 2.580293796690544 1105 98-99 2.960619488603573 1105 100-101 2.713482854848209 1106 1 0.06038245181588309 1106 2 0.080286901010453 1106 3 0.17485277280444222 1106 4 0.09699324761860595 1106 5 0.15016444100825765 1106 6 0.06404667507995043 1106 7 0.06646621897403548 1106 8 0.013507408952513345 1106 9 -0.12553797459001004 1106 10-11 -0.027347446731191383 1106 12-13 -0.018755786964995025 1106 14-15 -0.04281870321876369 1106 16-17 0.05867062166601755 1106 18-19 -0.11631394272047402 1106 20-21 -0.15674241036432335 1106 22-23 -0.03129433316389907 1106 24-25 0.019734579858877055 1106 26-27 -0.040256974430910475 1106 28-29 -0.17837955681228124 1106 30-31 -0.04791613927206839 1106 32-33 -0.06276580318271385 1106 34-35 0.03239987838353642 1106 36-37 0.20123027560183004 1106 38-39 -0.07158669235757031 1106 40-41 0.09394867053886458 1106 42-43 0.17315620596129122 1106 44-45 0.14632220472520885 1106 46-47 0.05322965450252326 1106 48-49 0.10897402014540347 1106 50-51 0.0726675831274477 1106 52-53 0.12997348846622003 1106 54-55 0.4716348555338996 1106 56-57 0.4264494193071826 1106 58-59 0.5751558498230693 1106 60-61 0.3985580750987836 1106 62-63 0.2819229467546762 1106 64-65 0.35261511166819304 1106 66-67 0.43390969263839807 1106 68-69 0.25334119989040005 1106 70-71 0.3451239862295097 1106 72-73 0.5093968874024668 1106 74-75 0.6501081634256174 1106 76-77 0.7143571566473241 1106 78-79 0.5770291732296968 1106 80-81 0.7861628205137912 1106 82-83 0.7928561808639749 1106 84-85 0.6762359175466663 1106 86-87 0.7364351786055501 1106 88-89 1.0198134387263984 1106 90-91 0.8199649095803032 1106 92-93 0.6152477198018431 1106 94-95 0.9058151318622443 1106 96-97 1.202164059481646 1106 98-99 1.4563566364604448 1106 100-101 1.3614338371354933 1107 1 0.024963949613237446 1107 2 0.07353212861691816 1107 3 0.12379060931937147 1107 4 0.02801625300284627 1107 5 -0.029453767508627493 1107 6 -0.05342762741638296 1107 7 -0.1490403889554841 1107 8 -0.06229288473323891 1107 9 -0.0589006764989648 1107 10-11 0.043406346709822685 1107 12-13 0.07278606325527193 1107 14-15 0.2008425792090307 1107 16-17 0.15511212925289897 1107 18-19 -0.06520772637195904 1107 20-21 -0.07439781907699938 1107 22-23 -0.018460265616177196 1107 24-25 -0.08011368250039652 1107 26-27 0.12481399962685202 1107 28-29 0.11122396741679808 1107 30-31 0.13409560815818367 1107 32-33 0.047924358344452855 1107 34-35 0.31465146918138487 1107 36-37 0.27270849390824026 1107 38-39 0.3336555983913243 1107 40-41 0.1847660958937425 1107 42-43 0.09659722407190685 1107 44-45 0.3193177994388705 1107 46-47 0.5995732668373321 1107 48-49 0.6347253662051173 1107 50-51 0.7387272992312219 1107 52-53 0.6352794111289697 1107 54-55 0.663783656317058 1107 56-57 0.7256417834775206 1107 58-59 1.0379850226081828 1107 60-61 1.1392678156763694 1107 62-63 1.1212474695153247 1107 64-65 0.9129773241008863 1107 66-67 0.8802484102106014 1107 68-69 0.6052844206441961 1107 70-71 0.6025346568119865 1107 72-73 0.37862840967363454 1107 74-75 0.33255064996501815 1107 76-77 0.29146924671095675 1107 78-79 0.8275724918787404 1107 80-81 0.6255656594761021 1107 82-83 0.98970884850959 1107 84-85 1.0089623003171013 1107 86-87 0.8738458491880259 1107 88-89 1.1431614563475456 1107 90-91 1.105373622257737 1107 92-93 1.0452868779026758 1107 94-95 0.7093099923615078 1107 96-97 0.7916794779838483 1107 98-99 0.7048098914775771 1107 100-101 0.9415659957258029 1108 1 -0.18056796528037466 1108 2 -0.05341822599301338 1108 3 0.002324888279176207 1108 4 -0.03245656023592147 1108 5 -0.10661688807600456 1108 6 0.0373525144275888 1108 7 -0.06989145278527076 1108 8 -0.18768295565522664 1108 9 -0.18074464813017244 1108 10-11 0.03253164221810323 1108 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24.0 6 55.0 7 118.0 8 197.0 9 390.0 10 696.0 11 1272.0 12 1815.0 13 2590.0 14 3448.0 15 4451.0 16 5725.0 17 7698.0 18 7320.0 19 4931.0 20 4108.0 21 4053.0 22 4402.0 23 4576.0 24 4868.0 25 5380.0 26 5243.0 27 5277.0 28 6274.0 29 7299.0 30 8654.0 31 10614.0 32 13033.0 33 16944.0 34 24760.0 35 45614.0 36 52199.0 37 88844.0 38 73133.0 39 5145.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.87244524023787 24.74394418725473 16.244932228706084 18.138678343801313 2 14.120725288846725 25.401346752924418 40.30493306828172 20.172994889947137 3 17.442796339893537 32.0428636042075 32.43392440882786 18.080415647071103 4 10.195845587212053 22.55517724443125 39.93774874987244 27.311228418484262 5 8.7866720166623 42.933251073284886 35.668544576983116 12.6115323330697 6 22.963667358606536 43.213693451311016 18.270459579501573 15.552179610580882 7 21.60842015419013 39.21132954197552 23.33563721715574 15.844613086678603 8 23.639935429403742 35.727903589420066 21.54069524719591 19.091465733980275 9 24.768065387006093 19.01979793856516 23.594244310643944 32.6178923637848 10-11 21.900541799255954 30.790247613392836 27.93327705053391 19.3759335368173 12-13 23.840095000417485 30.989827347874087 28.132160981176185 17.037916670532244 14-15 21.131330655261667 31.859930048520724 28.52633385595933 18.482405440258283 16-17 16.069473693976192 35.12580133408789 29.956442679679746 18.848282292256165 18-19 15.628797929287774 32.624154598335636 34.9734202933509 16.773627179025688 20-21 16.942707511898245 31.19891640148809 35.177754687398526 16.680621399215134 22-23 16.943171381124234 31.639476198870014 35.21753147352698 16.19982094647877 24-25 16.811550504464805 32.94305926011829 34.865476052417954 15.379914182998956 26-27 15.88166926876118 32.73616617524585 36.15167620510227 15.230488350890706 28-29 15.017418289435842 32.6333160155489 36.27608104723116 16.073184647784096 30-31 15.831392813737951 32.16040133965433 36.00993607882066 15.998269767787065 32-33 15.385043587980224 33.30001078537225 35.581406946011676 15.73353868063585 34-35 16.02857855332672 32.660507895637245 35.76320627243573 15.547707278600301 36-37 16.260832049878456 31.92358185967972 35.53271882132453 16.282867269117293 38-39 15.30392097729814 32.88266761934368 35.473686164067686 16.3397252392905 40-41 15.174836855279672 32.23340656383465 36.48966846765756 16.10208811322812 42-43 15.474862149516449 32.041393420756556 35.23903749393968 17.244706935787306 44-45 15.372241474491902 31.851604433125036 35.32710302051875 17.449051071864314 46-47 16.590320283707026 31.86728949852603 34.63779343104124 16.904596786725705 48-49 16.026449823265825 31.560618430452088 34.59443913571886 17.81849261056323 50-51 14.880692835075934 31.729234894098656 35.27076046720909 18.119311803616327 52-53 15.652919129039141 32.56605497778066 34.51082670773455 17.27019918544564 54-55 15.727254172503688 31.62034159329802 34.35380697473768 18.298597259460614 56-57 16.204807540658138 31.846013971741087 33.59549675755411 18.353681730046663 58-59 16.03769865199603 31.419834120364786 34.4216478490384 18.120819378600785 60-61 16.234031301895367 32.82999656736772 33.42769206505302 17.50828006568388 62-63 15.380279991464807 32.75647329504866 33.94850123853083 17.9147454749557 64-65 15.397907022052342 32.757285066194136 33.26603363979627 18.57877427195725 66-67 15.601502015521138 32.6334673921615 32.3173390482287 19.447691544088656 68-69 15.47521303293023 31.837927979329894 33.19382401647671 19.493034971263167 70-71 16.419578992290493 31.643766989210402 32.81074599448923 19.12590802400987 72-73 16.30059653582462 30.82724118416536 33.20236758852944 19.669794691480575 74-75 15.637147575355556 32.06020558684096 31.982971360713986 20.3196754770895 76-77 15.658485559750995 31.130147788735403 33.022154394233176 20.189212257280428 78-79 15.743025726187273 30.81749993041962 32.82269062705842 20.61678371633469 80-81 16.28801276572551 30.841032622876487 32.0356160030801 20.835338608317898 82-83 16.08056110272271 30.57455508626908 32.648538447086985 20.696345363921225 84-85 15.59007414125225 30.517017232361443 32.31262024903946 21.58028837734685 86-87 15.559647965153701 30.85546694251775 32.519734988130594 21.065150104197954 88-89 15.893551290020316 30.38389817142751 31.873498223380864 21.849052315171306 90-91 15.962577977044393 29.626232063395236 32.582488376651014 21.828701582909357 92-93 16.47114488094741 30.122995495808922 32.32939970405006 21.076459919193606 94-95 15.946682809334451 29.273893061783973 32.73888423086334 22.04053989801823 96-97 15.824666709961127 29.39574075276698 32.74267318557552 22.03691935169637 98-99 15.699537986250917 29.682875803653435 32.08281921160786 22.534766998487786 100-101 15.630649906702246 28.929029923703425 32.880934902637506 22.559385266956824 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7491.0 1 4376.0 2 1524.5 3 1637.5 4 1224.0 5 1302.5 6 2579.0 7 4670.0 8 6048.5 9 5875.0 10 4256.0 11 2669.5 12 2274.0 13 2117.5 14 1915.5 15 1662.5 16 1444.5 17 1395.5 18 1374.5 19 1419.5 20 1574.5 21 1702.0 22 1803.5 23 1966.0 24 2149.0 25 2624.5 26 3658.0 27 4624.0 28 4844.5 29 5364.5 30 6943.0 31 8524.5 32 10605.0 33 13512.5 34 16068.5 35 18235.5 36 21389.5 37 23364.5 38 22325.0 39 20735.5 40 19247.5 41 17670.5 42 16084.0 43 15629.5 44 15379.0 45 14521.0 46 12982.5 47 12592.5 48 14855.0 49 13636.0 50 9416.0 51 6300.0 52 3951.0 53 2703.5 54 2134.5 55 1719.0 56 1349.5 57 1071.5 58 908.0 59 784.5 60 615.0 61 467.5 62 349.0 63 219.5 64 134.5 65 102.5 66 97.0 67 102.5 68 85.0 69 70.0 70 66.5 71 56.5 72 51.0 73 48.0 74 52.0 75 57.0 76 42.0 77 21.0 78 14.0 79 17.0 80 14.5 81 8.0 82 2.5 83 1.5 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010437057584725715 3 0.004870626872872 4 0.0 5 6.958038389817142E-4 6 2.3193461299390476E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013916076779634285 26-27 0.021569919008433143 28-29 0.0 30-31 0.0 32-33 0.003826921114399429 34-35 0.014727847925112952 36-37 0.006494169163829334 38-39 0.02087411516945143 40-41 0.02249765746040876 42-43 0.01693122674855505 44-45 0.00881351529376838 46-47 0.0016235422909573335 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.638692259878095E-4 68-69 4.638692259878095E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.479019194908571E-4 82-83 0.0011596730649695239 84-85 0.004522724953381143 86-87 0.0012756403714664763 88-89 0.0 90-91 0.004522724953381143 92-93 4.638692259878095E-4 94-95 1.1596730649695238E-4 96-97 0.0 98-99 0.0 100-101 0.002203378823442095 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 431156.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.876775821802568 #Duplication Level Percentage of deduplicated Percentage of total 1 70.47110123268877 16.826226860484645 2 12.03570070943973 5.747474553952052 3 4.724171359949789 3.383939415159039 4 2.5435980185997544 2.4293167868355017 5 1.61603089991828 1.9292803759227317 6 1.104236489395181 1.5819364266925804 7 0.8219376202383335 1.3737654208555607 8 0.6354050808954049 1.2137139738059128 9 0.533660400073078 1.146788076376652 >10 4.384992655518308 22.119076478055273 >50 0.7062272063291763 11.758924138770352 >100 0.39401094138421555 16.425863264169465 >500 0.014962440812058818 2.536985739410633 >1k 0.009974960541372547 4.344457085297536 >5k 0.0039899842165490185 7.182251404212078 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8845 2.0514616519310875 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8433 1.955904591377599 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7339 1.7021681247622669 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5539 1.2846858213732386 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2626 0.6090602937219939 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2589 0.6004787130412195 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2350 0.5450463405356762 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2298 0.5329857406599932 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1682 0.39011401905574783 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1596 0.370167642338272 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1422 0.32981101967733256 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1301 0.3017469315050701 TruSeq Adapter, Index 11 (95% over 21bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1190 0.2760021894627467 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1187 0.2753063856237649 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 904 0.2096688901464899 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 902 0.20920502092050208 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 775 0.1797493250702762 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 764 0.17719804432734323 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 725 0.16815259442058095 RNA PCR Primer, Index 11 (95% over 23bp) TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 703 0.16305003293471504 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 702 0.16281809832172114 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 699 0.16212229448273943 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 677 0.15701973299687352 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 673 0.15609199454489792 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 670 0.1553961907059162 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 648 0.15029362922005027 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 627 0.14542300234717828 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 608 0.14101624470029409 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 575 0.13336240247149522 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 471 0.10924120272012915 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 453 0.10506637968623886 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 452 0.10483444507324495 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 444 0.10297896816929371 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 440 0.10205122971731809 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 437 0.10135542587833637 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1596730649695238E-4 0.0 12-13 0.0 0.0 0.0 3.479019194908571E-4 0.0 14-15 0.0 0.0 0.0 4.638692259878095E-4 0.0 16-17 0.0 0.0 0.0 6.958038389817142E-4 0.0 18-19 0.0 0.0 0.0 0.0011596730649695236 0.0 20-21 0.0 0.0 0.0 0.001855476903951238 0.0 22-23 0.0 0.0 0.0 0.004754659566375047 0.0 24-25 0.0 0.0 0.0 0.014843815231609905 0.0 26-27 0.0 0.0 0.0 0.03571793040106133 0.0 28-29 0.0 0.0 0.0 0.07143586080212266 0.0 30-31 0.0 0.0 0.0 0.14785831578361427 0.0 32-33 0.0 0.0 0.0 0.2697399549119112 0.0 34-35 0.0 0.0 0.0 0.4403278627689282 0.0 36-37 0.0 0.0 0.0 0.6743498872797781 0.0 38-39 0.0 0.0 0.0 0.9815472821902049 0.0 40-41 0.0 0.0 0.0 1.2958186827969458 0.0 42-43 0.0 0.0 0.0 1.575299891454601 0.0 44-45 0.0 0.0 0.0 1.8882956516898757 0.0 46-47 0.0 0.0 0.0 2.282004657247029 0.0 48-49 0.0 0.0 0.0 2.627123361381959 0.0 50-51 0.0 0.0 0.0 2.987897651893978 0.0 52-53 0.0 0.0 0.0 3.3426416424681555 0.0 54-55 0.0 0.0 0.0 3.714200892484391 0.0 56-57 0.0 0.0 0.0 4.128436111291505 0.0 58-59 0.0 0.0 0.0 4.53420571672434 0.0 60-61 0.0 0.0 0.0 4.944266112497564 0.0 62-63 0.0 0.0 0.0 5.334380131553313 0.0 64-65 0.0 0.0 0.0 5.743860690794051 0.0 66-67 0.0 0.0 0.0 6.167953130653406 0.0 68-69 0.0 0.0 0.0 6.64991325645474 0.0 70-71 0.0 0.0 0.0 7.167939214576626 0.0 72-73 0.0 0.0 0.0 7.6372589039697925 0.0 74-75 0.0 0.0 0.0 8.130235923888337 0.0 76-77 0.0 0.0 0.0 8.639448366716454 0.0 78-79 0.0 0.0 0.0 9.232621139448366 0.0 80-81 0.0 0.0 0.0 9.840405792798894 0.0 82-83 0.0 0.0 0.0 10.41363218881333 0.0 84-85 0.0 0.0 0.0 10.985698911762796 0.0 86-87 0.0 0.0 0.0 11.572725417250368 0.0 88-89 0.0 0.0 0.0 12.183293285956823 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCAACG 15 6.616157E-4 94.99617 3 TATCACG 20 0.0020725722 71.255394 2 TCACGCA 20 0.0020735261 71.24713 4 ATCACGC 25 0.0050199535 56.997704 3 GTAAGCG 30 1.4922625E-6 47.498085 94-95 AACGCGA 30 8.290997E-5 39.581738 76-77 CGGCTGC 30 8.290997E-5 39.581738 90-91 GTCGTAT 25 0.0016159118 37.99847 10-11 GGTACTC 65 1.3271232E-4 36.536987 3 TAAGGTG 80 1.0763448E-5 35.623566 5 GTAAGGT 80 1.0763448E-5 35.623566 4 ACGCGAA 35 2.0533937E-4 33.927208 78-79 GCCGGCT 35 2.0533937E-4 33.927208 88-89 CGCGAAT 35 2.0533937E-4 33.927208 78-79 GTATAGG 240 0.0 33.648384 1 GTGGCTC 75 3.0760077E-4 31.665392 9 TCATAGG 105 0.0 31.665392 94-95 CATATAG 90 2.4101555E-5 31.66539 3 TAAGAGG 60 0.0039490163 31.66539 4 ATGGGGG 655 0.0 29.731628 5 >>END_MODULE